92 results about "Gold Colloid" patented technology

The invention relates to the fields of analysis and test, in particular to a multi-channel microfluidic chip capable of detecting various subtype swine influenza viruses (SIV) simultaneously. One of the aims of advancing the related diagnosis and treatment technology is to diagnose the SIV quickly and cheaply. The invention provides a diagnosis device, which can fulfill the aims. The key point of the scheme is that: the device, namely the microfluidic chip, comprises pipelines with a parallel structure, wherein the parallel structure comprises three branched pipelines arranged in parallel; three beads-shaped working electrodes are arranged in the three branched pipelines respectively; each beads-shaped working electrode consists of a conductive electrode and a gold colloid sensitive film; the gold colloid sensitive film is attached to the conductive electrode and embeds different subtypes of SIV specific antibodies; the gold colloid sensitive films on the surface layers of the three beads-shaped working electrodes embed three different subtypes of SIV specific antibody substances respectively; and the three different subtypes of SIV specific antibody substances are the subtype H1N1, the subtype H3N2 and the subtype H1N2 SIV specific antibody substances respectively.

Recombinant B. burgdorferi proteins, representative of the life cycle, are membrane-immobilized to capture antibodies in biological samples. Lateral flow technology incorporating gold colloid deposition results in band visualization indicative of a positive test.

The present invention discloses a microwave producing method for nano-silver or gold; the reactants are added into the reactor according to the proportion of 1 to 1; the reactants are silver nitrate or auric chloride acid and starch or sodium carboxymethyl cellulose; the reactor is put into a microwave oven; with a mixer of a rotation speed of 100/min and the microwave power of 0.3 Kw, the reaction time of the reactant starch is one hour while the time of the reactant sodium carboxymethyl cellulose is seven hours; the solution is dried by air or vacuum freeze or spraying after the reaction is over to produce nano-silver or gold powder or concentrated to nano-silver or gold colloid of the needed consistency through vacuum concentration. The present invention discards the ordinary reducing agent in thermochemical synthesis and takes the microwave heating to replace the traditional local heating method and realize homogeneous growing of nano-silver or gold; the grain size distribution of the nano-silver and gold is narrow and the grain shapes are regular; the method is widely used in biochemical and antiseptic material field.

An apparatus and method for imaging metallic nanoparticles is provided. Preferably, the invention provides for an apparatus and method for detection of gold colloid particles and for accurate reporting to the operator. The apparatus includes a substrate holder for holding the substrate, a processor and memory device, an imaging module, an illumination module, a power module, an input module, and an output module. The apparatus may have a stationary substrate holder and imaging module which are proximate to one another. The apparatus provided for a compact sized system without the need for complex motorized devices to move the camera across the substrate. Further, the apparatus and method provide for automatic detection of the spots/wells on the substrate, automatic quantification of the spots on the substrate, and automatic interpretation of the spots based on decision statistics.

There is disclosed an approach for the gold-coating of cores, such as magnetic nanoparticles. In some instances, the core and gold colloids can be fabricated first through irradiation, such as laser irradiation, and then mixed together for further laser irradiation. Alternatively, the cores may be fabricated using wet chemistry and subsequently coated using an irradiation method. Also disclosed is a two phase aqueous:oil system and its use in coating a material present in one phase with a second material present in the second phase.

There is provided a lateral flow assay device for detecting the presence or quantity of an analyte residing in a test sample where the lateral flow assay device has a porous membrane in liquid communication with a conjugate pad and a wicking pad. The conjugate pad has a gold colloid-containing detection probe. The porous membrane has a detection zone where an immobilized first capture reagent configured to bind to at least a portion of the analyte and analyte-conjugate complexes to generate a detection signal. The control zone is located downstream from the detection zone on the porous membrane and has a second capture reagent immobilized within the control zone. The conjugate pad is located upstream from the detection zone, and has detection probes with specific binding members for the analyte. A sample containing an analyte is deposited on the conjugate pad, interacts with the detection probes, and moves toward the control zone for detection.

It is an object of the present invention to provide a method for producing gold colloid having a targeted particle size, a sharp particle size distribution and a uniform and perfect spherical shape. The present invention relates to a method for producing gold colloid including a nucleation step of forming nuclear colloidal particles by adding a first reducing agent to a first gold salt solution; and a growth step of growing nuclear colloid by adding a second gold salt and a second reducing agent to the solution of the nuclear colloidal particles, characterized in that the growth step is performed at least once; a citrate is used as the first reducing agent and an ascorbate is used as the second reducing agent; and the addition of the ascorbate in the growth step is performed simultaneously with addition of the second gold salt. According to the method for producing gold colloid of the present invention, gold colloid having a sharp particle size distribution and a uniform and perfect spherical shape can be obtained.

The invention discloses a sulfur-indium-zinc, gold and carbon nitride two-dimensional lamellar composite photocatalyst preparation method. The method includes: dissolving C6H5Na3O7 2H2O into dissolvedwater, diluting HAuCl4, heating to boil, adding sodium citrate solution, boiling, and cooling to the room temperature to obtain gold colloid mixed solution; dissolving g-C3N4 into ethyl alcohol, performing ultrasonic treatment, dissolving Zn(NO3)2 6H2O, In(NO3)3 4.5H2O and L-Cysteine into glycerin and the gold colloid mixed solution, mixing with g-C3N4 solution, and adsorbing to obtain ZnIn2S4/Au/g-C3N4 precursor solution; performing heating reaction in a hydrothermal reaction kettle, and performing vacuum freeze drying to obtain powdered sulfur-indium-zinc/gold/carbon nitride two-dimensionallamellar composite photocatalyst. By adoption of the method, easiness in raw material acquisition, one-kettle synthesis, high reliability and simplicity and convenience in operation are realized, andthe catalyst has high photocatalytic activity in a visible region and has a promising application prospect.

A total reflection cell has a total reflection prism on at least one surface thereof. A mixed solution containing a gold colloid labelled antibody which is adsorbed on a gold colloid is stored in the total reflection cell, and a sample solution containing an antigen causing antigen-antibody reaction with the antibody is added thereto for forming a gold colloid labelled immune complex. A measuring beam is introduced into the total reflection prism from an incident optical system at an angle θ of incidence causing total reflection and an outgoing beam from the total reflection prism is received by a measuring optical system, thereby measuring absorption by the gold colloid labelled immune complex and carrying out qualification and determination of the antigen.

The invention discloses a xanthan gum-silver nanoparticle composite material and a preparation method of the xanthan gum-silver nanoparticle composite material, and belongs to the technical field of nanometer materials. The composite material contains xanthan gum and nano-silver, the xanthan gum with high biocompatibility serves as a carrier, the dispersibility of the nano-silver is improved, and the antibacterial property and the biocompatibility of the composite material are improved. According to the preparation method of the xanthan gum-silver nanoparticle composite material, a silver colloid is prepared in a gold colloid system, silver covers gold particles for growth, a nano-silver colloid is prepared, the size and the height of the nano-silver particles are uniform, the nano-silver particles can be uniformly dispersed in a water solution for a long time, the nano-silver particles prepared through the method and generally-prepared nano-silver represent the same property, the nano-silver particles are mixed with the xanthan gum and then uniformly distributed on the xanthan gum of a reticular structure, the dispersibility is good, and the antibacterial property can be improved.

There are disclosed metallic colloid particles containing platinum fine particles wherein platinum colloid particles are supported on the surfaces of gold colloid particles, and the platinum fine particles have an average particle diameter of at most 5 nm; a process for producing the metallic colloid particles; a metallic corpuscular carrying body wherein the above metallic colloid particles are supported on a carrier and a process for producing the carrying body. The resultant metallic colloid particles, which have higher sensitivity, are well suited as a label for immunological measurement and protein-staining agent for various proteins.

A gold colloidal solution is applied by dripping to a radio-transparent substrate having grooves with a high aspect ratio. The applied gold colloidal solution flows into the groove by capillarity, and is retained in the bottom of the groove. The radio-transparent substrate is heated from beneath by a laser beam at a part of the groove to which the gold colloidal solution has been applied, so the gold colloidal solution is vaporized and dried. Thus, gold colloidal particles remaining in the groove form a seed layer for electrolytic plating.

The invention belongs to the laboratory medicine technology field, which relates to a device and a method of utilizing saliva to execute clinical or non-clinical detecting HIV/AIDS virus. The device of utilizing saliva to execute clinical or non-clinical detecting HIV/AIDS virus includes a strip shape test kit, a columned liquid collecting filter, a piston, a sampling cup, a measuring pipette, phosphate buffer reagent and antihuman IgG antibody-gold colloid conjugate reagent. The invention also provides a method of using the device to execute clinical or non-clinical detecting for saliva. Through detecting the saliva sample, the invention can achieve the requirements of accurate, quick, convenient and secure clinical or non-clinical detecting of HIV/AIDS virus.

An apparatus and method for imaging metallic nanoparticles. The invention teaches an apparatus and method for detection of gold colloid particles and for accurate reporting to the operator. The apparatus includes a substrate holder for holding the substrate, a processor and memory device, an imaging module, and illumination module, an input module, and an output module. The apparatus may have a stationary substrate holder and imaging module which are proximate to one another. The apparatus provides for a compact system without the need for complex motorized devices to move a camera across the substrate. Further, the apparatus and method provide for automatic detection of the spots/wells on the substrate, automatic quantification of the spots on the substrate, and automatic interpretation of the spots based on decision statistics.

The invention discloses a method for preparing a carbon nitride tube-polyaniline-gold composite material and an application method thereof. The method comprises the following steps: the acidified carbon nitride tube solution is alternatively treated by aqueous solutions, in which polydiene propyl dimethyl ammonium chloride and polystyrene sodium sulfonate sodium chloride serve as solutes respectively, and is then dissolved in hydrochloric acid solution; aniline monomer is added into the mixed solution, and hydrochloric acid solution serving as an oxidant and containing ammonium persulphate is added after the carbon nitride tube solution and the hydrochloric acid solution are uniformly mixed; carbon nitride tube-polyaniline is gained by washing, centrifuging and drying a reaction product obtained after the carbon nitride tube solution is reacted with the ammonium persulphate for 24 hours, and is dispersed in gold colloid; and carbon nitride tube-polyaniline-gold is obtained by centrifuging and drying a reaction product obtained after the carbon nitride tube-polyaniline is reacted with the gold colloid for 30 minutes. The pipe diameter of the two prepared composite materials is 60 nm, the carbon nitride tube-polyaniline and a modified electrode compose a dopamine biosensor, the detectability of the dopamine biosensor is 0.01 MuM, and the linearity range of the dopamine biosensor is 1-80 MuM and 1.5-3.5 mM. The carbon nitride tube-polyaniline-gold and a modified electrode compose a peroxide biosensor, the detectability of the peroxide biosensor is 1.4 MuM, and the linearity range of the peroxide biosensor is 0.02-2.05 mM.

The invention discloses an immunity chromatographic test paper for detecting ricin and a preparation method thereof. The immunity chromatographic test paper for detecting the ricin provided by the invention comprises an absorbent paper washer, a fiber membrane which is closely connected to the absorbent paper washer and coated with anti-ricin A-chain protein antibodies, a gold conjugate pad which is closely connected on the fiber membrane, consists of anti-ricin B-chain protein antibodies and is immunized from gold colloid probes, and a sample washer which is closely connected to the gold conjugate pad. Moreover, a backboard is also closely connected under the absorbent paper washer for convenience in use. By adoption of the immunity chromatographic test paper for detecting the ricin, laypeople can operate according to specifications; treatment processes of specimen is simple, and results can be observed within 5 minutes; at least 20 nanogram per milliliter ricin can be detected, and detection sensibility is not affected by specimen sources. The ricin immunity detection test paper has the advantages of simplicity, convenience, sensitivity, specificity and quickness and is suitable for clinical and field use.

The invention discloses a preparation method of gold nanoparticles. The preparation method comprises the following steps that a chloroauric acid solution with the concentration of 0.05-10 mmol/L and a HEPES buffer solution with the concentration of 5-50 mmol/L are prepared respectively, and the PH value of the HEPES buffer solution is adjusted to be 7.0-8.0 through sodium hydroxide; surfactants are added into the HEPES buffer solution, and a surfactant solution with the concentration of 1-2 mmol/L is prepared; the prepared chloroauric acid solution is slowly added into a reaction tank according to the mole ratio of 1:1-1: 10 of the chloroauric acid solution and the surfactant solution, and is stirred for 5-30 min at a constant speed of 200-300 r/min, and a mixed solution containing nano gold colloid is obtained; dry purification of the nano gold colloid is carried out on the mixed solution obtained in the step S400, and the gold nanoparticles are obtained. The gold nanoparticles can be prepared at normal temperature, and are good in dispersity and homogeneous degree.

The present invention relates to a kind of colloidal gold immunochromatography test paper strip and its preparation method and application, comprise rubber strip, described rubber strip comprises successively overlapped sample pad, colloidal gold pad, nitrocellulose membrane and water-absorbing filter paper, wherein The colloidal gold pad is embedded with a gold-labeled cryptococcal antibody, the nitrocellulose membrane is coated with a cryptococcal antibody as a detection line, and the antibody coated with an anti-virus antibody on the colloidal gold is used as a quality control line. Colloidal gold immunochromatographic test strips have good sensitivity, specificity, repeatability and stability, and have a high recovery rate of target compounds, which can provide more accurate and reliable test results.

The invention relates to a nano-antibacterial fabric. The nano-antibacterial fabric is manufactured by the following steps: mixing a 0.01-1g/L silk fibroin solution and a 0.01-100g/L chloroauric acid solution in a volume ratio of 10:1 to 1:1, adjusting the pH value of the mixture to be equal to 10, performing ultrasonic treatment at the temperature of 20-80 DEG C for 1-10 hours to obtain a nano gold colloid solution, immersing a fabric into a nano gold solution with concentration of 0.01-10g/L, and dipping twice and rolling twice. According to the invention, fibroin is taken as a reducing agent and a stabilizer of nano gold, thereby achieving the advantages of simple process, mild reaction, environmental friendliness and the like. The antibacterial fabric manufactured by dipping twice and rolling twice can have the antibacterial rate of 99%, and is excellent in washability.

The invention relates to a colloidal gold test paper bar for antibody detection of sheep Brucella. In the invention, lipopolysaccharide (LPS) extracted from a Brucella vaccine strain (S2) is taken as an envelope antigen of the colloidal gold test paper bar and improves the sensitivity of detection. Monoclonal-antibody-labeled colloidal gold of a mouse anti-sheep IgGFc terminal is prepared into a gold colloid pad of the colloidal gold test paper bar for antibody detection, and nonspecific adsorption of antibody protein by SPA in the tradition is changed. The monoclonal-antibody-labeled colloidal gold is taken as a colloidal gold labeled protein, so that specificity of detection is further improved. A set of serum for controlling quality of the test paper bar is prepared, and specificity and sensitivity of the test paper bar is ensured. The test paper bar can be used for on-site sampling, is convenient and rapid to use, and is quite suitable for application to a basic farm or a basic veterinarian.

The invention relates to a nano-gold antibacterial blended fabric. The nano-gold antibacterial blended fabric is manufactured by the following steps: mixing a 0.01-10g/L dialdehyde chitosan solution and a 0.01-100g/L chloroauric acid solution in a volume ratio of 10:1 to 1:1, performing ultrasonic treatment at the temperature of 20-80 DEG C for 1-10 hours to obtain a nano gold colloid solution, preparing a 0.01-10g/L nano gold solution, immersing a blended fabric into the nano gold solution, and dipping twice and rolling twice. According to the invention, dialdehyde chitosan is taken as a reducing agent and a stabilizer of nano gold, thereby achieving the advantages of simple process, mild reaction, environmental friendliness and the like. The antibacterial cotton/cashmere/spun silk fabric manufactured by dipping twice and rolling twice can have the antibacterial rate of 99%, and is excellent in washability.