Colloidal gold immunochromatographic test strip and preparation method and application thereof

A technology of immunochromatographic test paper and colloidal gold, which is applied in the direction of analyzing materials, measuring devices, instruments, etc., can solve the problems such as no detection method for cryptococcus

Pending Publication Date: 2018-03-06
DYNAMIKER BIOTECH TIANJIN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Specifically, the present invention relates to a kind of mouse IgG type monoclonal antibody BE-A6 and anti-Cryptococcus neoformans capsular polysaccharide GXM antigen for Cryptococcus neoformans capsular polysaccharide (Glucuronoxylomannan, GXM) a

Method used

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  • Colloidal gold immunochromatographic test strip and preparation method and application thereof
  • Colloidal gold immunochromatographic test strip and preparation method and application thereof
  • Colloidal gold immunochromatographic test strip and preparation method and application thereof

Examples

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Embodiment 1

[0080] The preparation of embodiment 1 colloidal gold immunochromatography test strip

[0081] Colloidal gold immunochromatography test strip of the present invention can be prepared according to the following method, specifically refers to the structure such as figure 1 Shown:

[0082] Paste the sample pad, colloidal gold pad, nitrocellulose membrane, and water-absorbing filter paper on one end of the polyvinyl chloride (PVC) rubber sheet, and then cut the PVC rubber sheet and the attached material into a width of 4± 0.2mm test strip, and put it into the card case, which has a sample adding area and a color developing area (observation area).

[0083] Wherein, the sample pad is a glass fiber membrane; wherein the gold-labeled cryptococcal antibody with a concentration of 2-8 μg / mL is embedded on the colloidal gold pad; the nitrocellulose membrane includes: the blocking line is HAMA blocking The mixture of HAMA inhibitor and HBR blocking agent, the concentration of the HAMA ...

Embodiment 2

[0103] Embodiment 2: the preparation of colloidal gold immunochromatography test strip

[0104] Compared with Example 1, in the preparation of the colloidal gold pad: the separation agent shown is 0.1% casein.

Embodiment 3

[0105] Embodiment 3: the preparation of colloidal gold immunochromatography test strip

[0106] Compared with Example 1, in the preparation of the colloidal gold pad: the separation agent shown is 0.25% casein.

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Abstract

The present invention relates to a kind of colloidal gold immunochromatography test paper strip and its preparation method and application, comprise rubber strip, described rubber strip comprises successively overlapped sample pad, colloidal gold pad, nitrocellulose membrane and water-absorbing filter paper, wherein The colloidal gold pad is embedded with a gold-labeled cryptococcal antibody, the nitrocellulose membrane is coated with a cryptococcal antibody as a detection line, and the antibody coated with an anti-virus antibody on the colloidal gold is used as a quality control line. Colloidal gold immunochromatographic test strips have good sensitivity, specificity, repeatability and stability, and have a high recovery rate of target compounds, which can provide more accurate and reliable test results.

Description

technical field [0001] The invention belongs to the technical field of immunological detection and analysis, and relates to a colloidal gold immunochromatographic test strip and its preparation method and application, in particular to a colloidal gold immunochromatographic test strip for detecting cryptococcus. Background technique [0002] Cryptococcus belongs to the subphylum Deuteromycota, Bacillus, Cryptococcus, and Cryptococcus family in fungal taxonomy. The cryptococci that cause human infection are mainly Cryptococcus neoformans and Cryptococcus gattii. The asexual propagules of the two cryptococci are all hyphae-free monospore yeast-like bacteria, which have no capsule or only a small capsule in vitro, and soon form a thick capsule after entering the human body. Capsulated cryptococcus The diameter of the bacteria increased significantly, and the pathogenicity was significantly enhanced. [0003] Cryptococcus (Crytococcus) is an important opportunistic pathogen that...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558
CPCG01N33/558G01N33/56911
Inventor 彭洁李昕彤李宁周泽奇粟艳
Owner DYNAMIKER BIOTECH TIANJIN
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