24 results about "Cryptococcus neoformans" patented technology

The present invention belongs to the field of biomedicine, and specifically relates to an antimicrobial peptide derived from lactoferrin and its application. The antimicrobial peptide derived from lactoferrin, whose amino acid sequence is shown in any one of SEQ ID NOs: 1 to 98, has a broad-spectrum antimicrobial effect, especially significant antibacterial effect against Pseudomonas putida, Rhodococcus erythropolis, Bacillus subtilis, Malassezia furfur, Cryptococcus neoformans, and Candida albicans, and has great application value.

The invention discloses a primer probe group, a kit and a detection method for simultaneously detecting five fungi. Specifically, in the primer and probe group, the nucleotide sequences of the primers comprise SEQ ID NO.7 to 8, SEQ ID NO.16 to 17, SEQ ID NO.25 to 26, SEQ ID NO.34 to 35 and SEQ ID NO.43 to 44, and the nucleotide sequences of the probes comprise SEQ ID NO.9, SEQ ID NO.18, SEQ ID NO.27, SEQ ID NO.36 and SEQ ID NO.45. The invention also discloses a kit for detecting the detection of the tumor cells. According to the invention, multiple detection in one tube is realized, and five invasive fungi, namely mucor, aspergillus, cryptococcus neoformans, pneumocystis yarrowii and talaromyces marneffei, are simultaneously detected in one tube of reagent by utilizing a PCR method.

The invention provides a tetrazole alcohol compound as well as a preparation method and application thereof, and belongs to the field of medicines. The tetrazole alcohol compound provided by the invention has the characteristics that the structural formula is as shown in the formula I: # imgabs0 #, in the formula I, R1 represents H or F, R2 represents H or F, R3 is independently selected from any one of C1-C4 alkyl, C1-C4 alkoxy, halogen, halogenated alkane, halogenated alkoxy, cyano, a benzene ring and a substituted benzene ring, R4 represents H or CH3, X represents CH or N, and Y represents CH or N. The azaconazole compound has remarkable in-vitro and in-vivo antifungal activity on human pathogenic fungi such as candida albicans, cryptococcus neoformans and aspergillus fumigatus, and the in-vitro and in-vivo antifungal activity is higher than that of a traditional antifungal drug fluconazole, and meanwhile, the azaconazole compound has a low inhibition effect on human CYP450 enzyme, especially on human liver drug enzyme CYP3A4, and has a good application prospect. Therefore, the compound can be used for preparing antifungal drugs.

The invention relates to application of azole drugs in preparation of antifungal drugs, and belongs to the technical field of research and development of antifungal drugs. According to the application of the glycyrrhetinic acid combined with the azoles in preparation of the antifungal drugs, the glycyrrhetinic acid combined with the itraconazole, the voriconazole and the posaconazole shows a good synergistic effect or antagonistic effect on aspergillus, ectobottle dermatitis, candida and cryptococcus neoformans, and the application of the glycyrrhetinic acid combined with the itraconazole, the voriconazole and the posaconazole in preparation of the antifungal drugs is disclosed. And a research and development direction is provided for preparing medicines for resisting aspergillus, ectobottle dermatitis, candida and cryptococcus neoformans.

The invention belongs to the technical field of biological detection, and particularly relates to a nitrogen-containing heterocyclic compound, a preparation method thereof, a fluorescent probe and application. The nitrogen-containing heterocyclic compound provided by the invention has a structure as shown in the following general formula (I): # imgabs0 #, is simple in structure, has good chemical and optical stability, can be used for imaging fungi such as cryptococcus neoformans, has application potential for judging fungal infection conditions, and in addition, has good application prospects. The compound can be combined with intein of fungi through sulfydryl reaction, shows good specificity as a targeted fluorescent probe, and realizes rapid evaluation and screening of Prp8 intein inhibitor candidate compounds.

This invention discloses the application of larvicin or its pharmaceutical salt in the preparation of antifungal drugs. The structure of larvicin is shown below. This invention discovers an effective antifungal compound with pharmaceutical potential: larvicin. The compound larvicin of this invention exhibits clear antifungal activity against pathogenic fungi such as *Candida albicans*, *Candida auris*, *Candida tropicalis*, *Candida parapsilosis*, *Candida krusei*, *Cryptococcus neoformans*, *Trichophyton mentagrophytes*, and *Trichophyton rubrum*, with a minimum inhibitory concentration (MIC). 90 The range is 2–8 μg / mL.

The invention provides two double-target antibacterial peptides as well as a preparation method and application thereof, and belongs to the field of bioengineering, the sequences of the double-target antibacterial peptides are shown as NLR-2 and NIR-3, and the N end of the double-target antibacterial peptides is connected with carboxyl of 3-(1-naphthyl) acrylic acid. The two antifungal peptides disclosed by the invention have good bactericidal activity (1 mu M) on cryptococcus neoformans under physiological conditions, and meanwhile, have a relatively good mildew-proof effect on corn feed, and can effectively reduce the toxin yield of the corn feed caused by fusarium graminearum. Therefore, the antifungal peptide provided by the invention has a good bactericidal function, can target a fungal membrane structure, achieves the effects of efficient membrane rupture and sterilization, and can be used as a novel mildew preventive.

This invention provides two dual-target antimicrobial peptides, their preparation methods, and applications, belonging to the field of bioengineering. Their sequences are shown as NLR-2 and NIR-3, with the N-terminus linked to the carboxyl group of 3-(1-naphthyl)acrylic acid. The two antifungal peptides of this invention exhibit good bactericidal activity against Cryptococcus neoformans under physiological conditions (1 μM), and also demonstrate good antifungal activity in corn feed, effectively reducing the toxin production in corn feed caused by Fusarium graminearum. Therefore, the antifungal peptides of this invention possess excellent bactericidal function, can target fungal membrane structures, achieving highly efficient membrane-breaking and bactericidal effects, and can be used as novel antifungal agents.

The invention relates to the technical field of fungal etiology detection, and discloses a method for accurately detecting cryptococcus neoformans, which comprises the following steps: S1, selecting a sample to be detected; s2, direct smear microscopic examination; s3, culturing; and S4, detecting. One of cerebrospinal fluid, blood and sputum of a patient is adopted as a sample to be detected, and timely detection or proper preservation is needed after collection. In the step S2, the sample is mixed with a proper amount of Indian ink, the sample is observed under a microscope, the capsule of the cryptococcus neoformans is found, and the cryptococcus neoformans can present obvious capsule and a transparent halo in a black background and can be easily recognized. And S3, inoculating the sample on a proper culture medium, and observing whether cryptococcus neoformans grow or not. The method adopted by the invention is rapid, simple and convenient, not only has higher sensitivity and specificity, but also can improve the accuracy and reliability of cryptococcus neoformans detection.

The invention relates to the technical field of biological detection, and discloses a composition for detecting respiratory tract infection pathogens, a kit and application. The composition disclosed by the invention can be used for rapidly and simultaneously detecting legionella pneumophila, bordetella pertussis, chlamydia pneumoniae, mycoplasma pneumoniae, mycobacterium tuberculosis, aspergillus and cryptococcus neoformans in a sample with high specificity, and has relatively high sensitivity and stability. Compared with traditional detection methods such as pathogen isolated culture and genome sequencing, the efficiency of identifying and jointly detecting multiple respiratory tract pathogens by adopting the composition disclosed by the invention is effectively improved, the detection process is simple and convenient to operate, and the composition is suitable for large-scale popularization and application.

The present invention discloses a sesterterpenoid compound, a preparation method thereof and an application thereof. In the present invention, two novel-structured sesterterpenoid compounds are obtained by co-culturing and fermenting two edible and medicinal fungi, Pleurotus ostreatus and Trametes robiniophila. The structural formulas are shown as Formula I or Formula II. The two sesterterpenoid compounds have significant activities of inhibiting Candida albicans and Cryptococcus neoformans, and can be applied to the research and development of antibacterial drugs; moreover, the preparation method described in the present invention opens up a new way for preparing new antibacterial preparations in the antibacterial field.

The invention provides an antibacterial peptide QS18 as well as a preparation method and application thereof, and belongs to the technical field of antibacterial peptides. Aiming at the public health problem of cryptococcus neoformans, the invention provides an antibacterial peptide QS18, and the amino acid sequence of the antibacterial peptide QS18 is shown as SEQ ID NO: 1. The antibacterial peptide QS18 has good inhibiting and killing effects on cryptococcus neoformans, is high in stability, has the advantages of low hemolytic activity and low cytotoxicity, is expected to become a novel candidate drug for resisting cryptococcus neoformans, and has a wide market application prospect.

The present invention relates to a screening method for blood-brain barrier crossing inhibitors against Cryptococcus neoformans, a screening method for antifungal agents, and an antifungal composition. Since the screening method of the present invention utilizes a novel gene that regulates the blood-brain barrier crossing of Cryptococcus neoformans, it is possible to effectively screen for blood-brain barrier crossing inhibitors and antifungal agents.

The present invention relates to antifungal oligopeptide compounds according to Formula I, Asp–R2–Gly–Leu–R5–R6–R7–Leu–Gly–R10–R11–R12 (Formula I), wherein R2 represents Arg, Lys, Cit, D-Arg or Orn; R5 represents Phe, Arg, Lys, His, Trp, Tyr, D-Tyr, D-Phe, Orn, 4-aminophenylalanine or 3-phenylpropionate; R6 represents Asp, Pro, Leu, Glu or Lys; R7 represents Phe or Trp; each of R10, R11, and R12 independently represents Lys, Arg or Orn; that are effective in therapeutic and research applications related to human pathogenic fungi, particularly yeasts Candida albicans and Cryptococcus neoformans. Additionally, the invention covers the use of these antifungal oligopeptides in the treatment of fungal infections, particularly those affecting the central nervous system (CNS) – by the administration of a therapeutically effective amount of any of these oligopeptide compounds.

Provided are: a novel therapeutic agent for fungal infections caused by Candida auris or Cryptococcus neoformans, which have acquired resistance to an azole-based antifungal agent; and a method for identifying resistant fungal strains, the method being performed prior to treatment with said therapeutic agent.　The therapeutic agent is characterized by combining an azole-based antifungal agent and milbemycin as active ingredients. The identifying method is characterized by using an azole-based antifungal agent-added and milbemycin-added fungal isolation medium and an azole-based antifungal agent-added and milbemycin-free fungal isolation medium.

The invention discloses application of laricilic acid or pharmaceutical salt thereof in preparation of antifungal drugs. The structure of the laricilic acid is shown as follows: # imgabs0. The invention finds an effective antifungal compound with patent medicine potential, namely the laricilic acid. The compound laricilic acid has a definite antifungal effect on pathogenic fungi such as candida albicans, candida auricula, candida tropicalis, candida parapsilosis, candida krusei, cryptococcus neoformans, trichophyton mentagrophytes and trichophyton rubrum, and the range of the minimum inhibitory concentration MIC90 is 2-8 micrograms per milliliter.

The present invention relates to a method for screening an antifungal agent against Cryptococcus neoformans and an antifungal composition. Utilizing a newly identified gene essential for the growth and survival of Cryptococcus neoformans, the screening method of the present invention can effectively screen an antifungal agent.

The application relates to the application of azole drugs in the preparation of antifungal drugs and belongs to the technical field of antifungal drug research and development. The application of glycyrrhetic acid combined with azole drugs in the preparation of antifungal drugs discloses that glycyrrhetic acid combined with itraconazole, voriconazole and posaconazole shows good synergistic effect or antagonistic effect on aspergillus, exophiala dermatiditis, candida and new cryptococcus, and provides a research and development direction for preparing drugs for resisting aspergillus, exophiala dermatiditis, candida and new cryptococcus.

The invention belongs to the technical field of microbial fermentation. The invention provides a mutant strain delta wbpO, which is obtained by knocking out a wbpO gene in stenotrophomonas maltophilia CGMCC (China General Microbiological Culture Collection Center) NO. 1.1788. The base sequence of the wbpO gene is as shown in SEQ ID NO. 1 in a sequence table. The invention also provides an outer membrane vesicle of the mutant strain delta wbpO. The outer membrane vesicle of the mutant strain [delta] wbpO provided by the invention has an effect of inhibiting or killing cryptococcus neoformans (C. neoformans).

The present application belongs to the field of molecular biology detection, and particularly relates to a composition, method and use for simultaneous detection and differentiation of Pneumocystis jirovecii, Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger, Lichtheimia ramosa, Cryptococcus neoformans, Candida albicans and Histoplasma capsulatum. The present application uses a multiplex PCR technique and a Qsep400 high-throughput analysis system, and the lengths of eight fungal amplification fragments are at least 20 bases apart, so that the target fragments can be accurately differentiated. Pneumocystis jirovecii, Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger, Lichtheimia ramosa, Cryptococcus neoformans, Candida albicans and Histoplasma capsulatum can be simultaneously detected in one tube of reaction solution. One sample only needs to be subjected to nucleic acid extraction and simultaneously subjected to single-tube PCR amplification, and the sample detection result can be obtained through band analysis on the Qsep400, and the PCR process is not interfered, and the detection result is accurate.

The invention discloses an application of natamycin acetate in preparation of a medicine for treating invasive fungal diseases. The invention provides application of natamycin acetate in treating diseases caused by invasive cryptococcus neoformans, such as cryptococcus neoformans meningitis, and a new treatment method is provided for the diseases caused by the invasive cryptococcus neoformans.

The application belongs to the field of biological medicine and particularly relates to a kind of detection of a variety of respiratory fungal convection amplification primer pair and probe and application. The convection amplification primer pair includes aspergillus fumigatus primer pair, cryptococcus neoformans primer pair and pneumocystis jirovecii primer pair, and the primer sequence in aspergillus fumigatus primer pair, cryptococcus neoformans primer pair and pneumocystis jirovecii primer pair includes target nucleic acid complementary sequence and interface sequence, and the interface sequence is not complementary with the target nucleic acid; folding sequence is provided in the target nucleic acid complementary sequence, so that the primer sequence has a curved hairpin structure. The primer probe is designed at the same time, and a respiratory fungal multiplex detection kit is prepared. The multiplex detection can avoid non-specific amplification and improve the detection sensitivity.

The present invention relates to a method for screening for an inhibitor against the blood-brain barrier passage of Cryptococcus neoformans, a method for screening for an antifungal agent, and an antifungal composition. The screening method of the present invention uses a novel gene that regulates the blood-brain barrier passage of Cryptococcus neoformans, and thus can effectively screen for an inhibitor against blood-brain barrier passage and an antifungal agent.

The invention belongs to the field of natural medicines and chemical medicines, and particularly relates to an imidazolyl-containing alkyne compound and application thereof in preparation of antifungal medicines. The imidazolyl-containing alkyne compound is a compound as shown in a formula (I) or a medicinal salt or a medicinal esterified derivative thereof. The alkyne compound containing the imidazolyl group is prepared by the following steps: crushing branches and leaves of Chieniodon hainanense (Merr.) Tsiamamp, performing percolation extraction by using ethanol, concentrating an extract, then extracting by using dichloromethane, and then performing silica gel column chromatography, octadecyl bonded silica gel column chromatography and preparative HPLC (High Performance Liquid Chromatography) separation and purification, thereby obtaining the alkyne compound containing the imidazolyl group. The imidazolyl-containing alkyne compound has remarkable antifungal activity on cryptococcus neoformans, candida albicans and fusarium solani at lower concentration, and can be used for preparing antifungal drugs or pesticides.