8 results about "Cryptococcus neoformans" patented technology

The invention relates to application of azole drugs in preparation of antifungal drugs, and belongs to the technical field of research and development of antifungal drugs. According to the application of the glycyrrhetinic acid combined with the azoles in preparation of the antifungal drugs, the glycyrrhetinic acid combined with the itraconazole, the voriconazole and the posaconazole shows a good synergistic effect or antagonistic effect on aspergillus, ectobottle dermatitis, candida and cryptococcus neoformans, and the application of the glycyrrhetinic acid combined with the itraconazole, the voriconazole and the posaconazole in preparation of the antifungal drugs is disclosed. And a research and development direction is provided for preparing medicines for resisting aspergillus, ectobottle dermatitis, candida and cryptococcus neoformans.

This invention provides two dual-target antimicrobial peptides, their preparation methods, and applications, belonging to the field of bioengineering. Their sequences are shown as NLR-2 and NIR-3, with the N-terminus linked to the carboxyl group of 3-(1-naphthyl)acrylic acid. The two antifungal peptides of this invention exhibit good bactericidal activity against Cryptococcus neoformans under physiological conditions (1 μM), and also demonstrate good antifungal activity in corn feed, effectively reducing the toxin production in corn feed caused by Fusarium graminearum. Therefore, the antifungal peptides of this invention possess excellent bactericidal function, can target fungal membrane structures, achieving highly efficient membrane-breaking and bactericidal effects, and can be used as novel antifungal agents.

The present invention relates to antifungal oligopeptide compounds according to Formula I, Asp–R2–Gly–Leu–R5–R6–R7–Leu–Gly–R10–R11–R12 (Formula I), wherein R2 represents Arg, Lys, Cit, D-Arg or Orn; R5 represents Phe, Arg, Lys, His, Trp, Tyr, D-Tyr, D-Phe, Orn, 4-aminophenylalanine or 3-phenylpropionate; R6 represents Asp, Pro, Leu, Glu or Lys; R7 represents Phe or Trp; each of R10, R11, and R12 independently represents Lys, Arg or Orn; that are effective in therapeutic and research applications related to human pathogenic fungi, particularly yeasts Candida albicans and Cryptococcus neoformans. Additionally, the invention covers the use of these antifungal oligopeptides in the treatment of fungal infections, particularly those affecting the central nervous system (CNS) – by the administration of a therapeutically effective amount of any of these oligopeptide compounds.

The application relates to the application of azole drugs in the preparation of antifungal drugs and belongs to the technical field of antifungal drug research and development. The application of glycyrrhetic acid combined with azole drugs in the preparation of antifungal drugs discloses that glycyrrhetic acid combined with itraconazole, voriconazole and posaconazole shows good synergistic effect or antagonistic effect on aspergillus, exophiala dermatiditis, candida and new cryptococcus, and provides a research and development direction for preparing drugs for resisting aspergillus, exophiala dermatiditis, candida and new cryptococcus.

The present application belongs to the field of molecular biology detection, and particularly relates to a composition, method and use for simultaneous detection and differentiation of Pneumocystis jirovecii, Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger, Lichtheimia ramosa, Cryptococcus neoformans, Candida albicans and Histoplasma capsulatum. The present application uses a multiplex PCR technique and a Qsep400 high-throughput analysis system, and the lengths of eight fungal amplification fragments are at least 20 bases apart, so that the target fragments can be accurately differentiated. Pneumocystis jirovecii, Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger, Lichtheimia ramosa, Cryptococcus neoformans, Candida albicans and Histoplasma capsulatum can be simultaneously detected in one tube of reaction solution. One sample only needs to be subjected to nucleic acid extraction and simultaneously subjected to single-tube PCR amplification, and the sample detection result can be obtained through band analysis on the Qsep400, and the PCR process is not interfered, and the detection result is accurate.

The invention discloses an application of natamycin acetate in preparation of a medicine for treating invasive fungal diseases. The invention provides application of natamycin acetate in treating diseases caused by invasive cryptococcus neoformans, such as cryptococcus neoformans meningitis, and a new treatment method is provided for the diseases caused by the invasive cryptococcus neoformans.

The application belongs to the field of biological medicine and particularly relates to a kind of detection of a variety of respiratory fungal convection amplification primer pair and probe and application. The convection amplification primer pair includes aspergillus fumigatus primer pair, cryptococcus neoformans primer pair and pneumocystis jirovecii primer pair, and the primer sequence in aspergillus fumigatus primer pair, cryptococcus neoformans primer pair and pneumocystis jirovecii primer pair includes target nucleic acid complementary sequence and interface sequence, and the interface sequence is not complementary with the target nucleic acid; folding sequence is provided in the target nucleic acid complementary sequence, so that the primer sequence has a curved hairpin structure. The primer probe is designed at the same time, and a respiratory fungal multiplex detection kit is prepared. The multiplex detection can avoid non-specific amplification and improve the detection sensitivity.

The invention belongs to the field of natural medicines and chemical medicines, and particularly relates to an imidazolyl-containing alkyne compound and application thereof in preparation of antifungal medicines. The imidazolyl-containing alkyne compound is a compound as shown in a formula (I) or a medicinal salt or a medicinal esterified derivative thereof. The alkyne compound containing the imidazolyl group is prepared by the following steps: crushing branches and leaves of Chieniodon hainanense (Merr.) Tsiamamp, performing percolation extraction by using ethanol, concentrating an extract, then extracting by using dichloromethane, and then performing silica gel column chromatography, octadecyl bonded silica gel column chromatography and preparative HPLC (High Performance Liquid Chromatography) separation and purification, thereby obtaining the alkyne compound containing the imidazolyl group. The imidazolyl-containing alkyne compound has remarkable antifungal activity on cryptococcus neoformans, candida albicans and fusarium solani at lower concentration, and can be used for preparing antifungal drugs or pesticides.