Fungus rapid culture, identification and susceptibility detection kit and detection method thereof

Abstract

The invention discloses a fungus rapid culture, identification and susceptibility detection kit for rapidly and effectively identifying multiple fungi and testing the susceptibility. The invention also discloses a method for directly detecting a clinical sample by using the kit. The kit has the advantages that the multiple fungi are simultaneously identified and different pores are favorable for growing different fungi by coating various sugars or inhibitors on a susceptibility test board, so that the most common fungi in clinic use, such as candida albicans, candida tropicalis, candida glabrata, candida krusei, cryptococcus neoformans, candida guilliermondii and the like, can be identified and effective guidance is provided for the clinical use. The detection method is simple and suitable for detecting pure fungi, can be further directly applied to fungus identification of the clinical sample and the susceptibility detection of antibiotics and has a short detection period, namely thefungi to be detected can be simultaneously identified and the susceptibility can be detected at one time and the result can be given in 24 hours; and thus the detection method greatly shortens the clinical detection period and is favorable for early diagnosis and early therapy of patients.

Description

technical field [0001] The invention belongs to the technical field of in vitro diagnosis, and in particular relates to a rapid culture and identification drug susceptibility kit for fungi, and also relates to a detection method using the kit. Background technique [0002] Fungi (fungus) originally belonged to the plant kingdom, but the modern classification system separates them into a kingdom. Fungi, unlike bacteria, have a true nuclear envelope, produce spores, lack chlorophyll, produce branched hyphae, and have a cell wall of chitin or cellulose. Clinically, pathogenic fungi are divided into two types: superficial fungi and deep fungi. The former invades the skin, hair, and nails and is obstinate to treatment, but has less impact on the body. The latter can invade the internal organs of the whole body, and severe cases can cause death. Deep fungi are fungi that invade the subcutaneous tissue and internal organs and cause systemic infections. These fungi are mainly Cand...

AI Technical Summary

Problems solved by technology

The diagnosis of clinical fungi is usually: first use plate culture for 1-3 days for purification and culture, then identify it by color-changing plate or automatic instrument, and then analyze its antibiotic sensitivity by MH plate method. More than 4 days, high requirements for operators, and troublesome, time-consuming and labor-intensive, high cost, not suitable for rapid clinical diagnosis and treatment, easy to delay the condition

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