13 results about "Candida nivariensis" patented technology

The invention relates to Penicillium decumbens C01 and application of the Penicillium decumbens C01, and belongs to the technical field of microorganisms. The penicillium decumbens C01 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the penicillium decumbens C01 is GDMCC NO.66456. The strain is obtained by separating, purifying and screening soil samples collected from botanical gardens in South China of Guangzhou; tests show that the bacteriostatic active substance generated by fermentation of the strain has a good bacteriostatic effect on staphylococcus epidermidis, staphylococcus aureus, escherichia coli, propionibacterium acnes, candida albicans and pseudomonas aeruginosa, and the bacteriostatic active substance has the characteristic of high temperature resistance and excellent preservative effect; the antibacterial preservative can be used as an antibacterial preservative raw material, is applied to the fields of food, health care products or daily chemical products and the like, and has wide application prospects and important conversion research values.

The invention relates to a Paenibacillus polymyxa strain B01 and application of the Paenibacillus polymyxa strain B01, and belongs to the technical field of microorganisms. The paenibacillus polymyxa B01 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the paenibacillus polymyxa B01 is GDMCC NO.66594. The strain is obtained by separation, purification and screening from a soil sample, and tests show that the strain has excellent pH and high temperature tolerance; antibacterial active substances generated by fermentation of the bacillus subtilis have a relatively good antibacterial effect on escherichia coli, pseudomonas aeruginosa, pseudomonas putida, staphylococcus aureus, candida albicans, aspergillus brazilis, propionibacterium acnes and malassezia; and the antibacterial active substance has excellent heat resistance, cold resistance, illumination stability, pH stability and preservative effect, belongs to a mild low-irritation raw material, can be used for preparing daily chemical products and medicines, and has wide application prospects and important conversion research values.

The present application relates to a strain of Penicillium decumbens (P. Penicillium decumbens ) C01 and its application, and belongs to the field of microbial technology. The Penicillium decumbens C01 disclosed by the present application has been preserved in the Guangdong Microbial Digital Culture Collection Center, and its preservation number is GDMCC NO.66456. The strain is isolated, purified and screened from a soil sample collected in the garden area of Guangzhou South China Botanical Garden. Tests show that the bacteriostatic active substance produced by fermentation of the strain has good bacteriostatic effect on Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Propionibacterium acnes, Candida albicans and Pseudomonas aeruginosa. Moreover, the bacteriostatic active substance has high-temperature resistance and excellent preservative effect, and can be used as a bacteriostatic and preservative raw material, applied in the fields of food, health care products or daily chemical products, and has broad application prospect and important transformation research value.

This invention relates to a polymyxin Bacillus strain ( Paenibacillus polymyxa This invention relates to B01 and its applications, belonging to the field of microbial technology. The *Bacillus polymyxa* B01 strain disclosed in this invention has been deposited at the Guangdong Provincial Microbial Culture Collection Center, with accession number GDMCC NO.66594. This strain was isolated, purified, and screened from soil samples. Experiments show that this strain has excellent pH and high-temperature tolerance, and the antibacterial active substances produced by its fermentation have good antibacterial effects against *Escherichia coli*, *Pseudomonas aeruginosa*, *Pseudomonas putida*, *Staphylococcus aureus*, *Candida albicans*, *Aspergillus brasiliensis*, *Propionibacterium acnes*, and *Malassezia*. Furthermore, the antibacterial active substances exhibit excellent heat resistance, cold resistance, light stability, pH stability, and preservative effects. It is a mild and low-irritant raw material that can be used to prepare daily chemical products and pharmaceuticals, possessing broad application prospects and significant value for transformational research.

The invention discloses a microbial agent for in-situ treatment of enteromorpha in an open sea area as well as a preparation method and application of the microbial agent. The microbial agent is naturally formed by fermentation of a composite flora under a proper condition, and the pH value of the microbial agent is less than 4.0; the composite flora comprises bacillus subtilis, nitrifying bacteria, actinomycetes, filamentous bacteria, photosynthetic bacteria, lactobacillus plantarum, saccharomyces cerevisiae, candida, lactobacillus paracasei and bacillus megatherium, and can be applied to differential in-situ treatment of enteromorpha in different scenes (beaches, sea floats and cabins) in open sea areas. The microbial agent is an ecological treatment microbial agent, an acidity chemical regulator is not added, the enteromorpha prolifera in the open sea area can be treated in situ in a strong mode within a short time, and no negative influence is caused to seawater and the marine environment.

The application provides a water-retaining soil conditioner containing high-efficiency degrading bacteria and a preparation method and application thereof, and belongs to the technical field of soil improvement.The water-retaining soil conditioner comprises a carboxymethylated modified humic acid-chitosan compound, modified attapulgite powder, a composite microbial inoculum, konjac glucosan, polyacrylic acid, bentonite, polyglutamic acid, sodium alginate, mushroom residue, seaweed residue and straw compost; the composite microbial inoculum is obtained by combination of Candida tropicalis, Bacillus smithii and Bacillus pasteurii.The application realizes precise adaptation of the carrier and the high-efficiency degrading bacterial group by optimizing the design of the composite water-retaining carrier and introducing specific bacterial combinations, strengthens the synergistic effect of water retention, bacteria fixation, slow release and soil improvement, realizes water retention, degradation and fertilization synergistic effect, and has important practical significance and application value for improving soil quality, guaranteeing crop production and promoting the development of ecological agriculture.

The invention provides a method for pretreating livestock and poultry manure by adopting a composite functional microbial agent and application, and belongs to the technical field of ecological agriculture. According to the invention, pretreatment with a composite functional microbial agent (bacillus subtilis, bacillus licheniformis and candida tropicalis are compounded according to a specific ratio) is combined with a hermetia illucens larva synergistic treatment process, so that the livestock and poultry manure recycling efficiency can be remarkably improved. According to the technology, the excrement reduction effect is greatly enhanced, emission of ammonia gas and greenhouse gas is effectively inhibited, and meanwhile high-nutritive-value insect powder and high-safety insect excrement products are produced. The comprehensive efficiency of the strain is clearly superior to that of single microbial inoculum fermentation, hermetia illucens independent treatment and a commercially available microbial inoculum control group, and the strain-insect synergistic system is proved to have outstanding advantages in low-carbon conversion and high-value utilization of feces.

The invention relates to Aspergillus oryzae C02 and an application of the Aspergillus oryzae C02, and belongs to the technical field of microorganisms. The aspergillus oryzae C02 disclosed by the invention is preserved in Guangdong Microbial Culture Collection Center, and the preservation number of the aspergillus oryzae C02 is GDMCC NO.66457. The strain is obtained by separating, purifying and screening soil samples collected from botanical gardens in South China of Guangzhou; tests show that bacteriostatic active substances generated by fermentation of the strain have good bacteriostatic effects on pseudomonas putida, escherichia coli, staphylococcus aureus, burkholderia cepacia, candida albicans and propionibacterium acnes, and the bacteriostatic active substances have the characteristic of high temperature resistance and excellent preservative effect; the antibacterial preservative can be used as an antibacterial preservative raw material, is applied to the fields of food, health care products or daily chemical products and the like, and has wide application prospects and important conversion research values.

This invention discloses a bacterial residue compound fermentation agent and its preparation method, belonging to the field of biotechnology. The fermentation agent is composed of *Bacillus subtilis*, *Lactobacillus plantarum*, and *Candida utilis* in a specific live bacteria ratio, and is synergistically formulated with cellulase, xylanase, laccase, and peroxidase. The total bacterial dry matter accounts for 35%–45%, and the compound enzyme system accounts for 55%–65%. The preparation method includes: step S1, bacterial culture and powder preparation; step S2, bacterial powder mixing and compounding; step S3, enzyme powder preparation; and step S4, mixing and sieving. This invention achieves efficient degradation of lignocellulose, enrichment of crude protein, and efficient chemical degradation of aflatoxin B1, zearalenone, and vomitoxin through dynamic pH control and metabolic flux distribution. When used for corn cob treatment, this fermentation agent significantly improves the production performance, nutrient digestibility, and antioxidant and immune functions of beef cattle.

The invention provides a rational design method for improving the hydrolytic activity of lipase, and belongs to the field of enzyme engineering. It is found through molecular simulation that whether the initial conformation of a substrate entering Tunnel is reasonable or not is likely to be an important factor affecting the activity of the substrate. On the basis, key residues at a Tunnel inlet of the lipase are recognized through CaverWeb, NNK degenerate codons are adopted for site-directed saturation mutation to establish a mutant library, and excellent mutants with improved hydrolytic activity are screened by adopting a microwell plate screening method based on a spectrophotometric method. The hydrolytic activity of a plurality of single-region mutants of the candida antarctica lipase B obtained by the invention on 4-p-nitrophenol palmitate at 40 DEG C is improved by more than 10 times compared with that of a wild type; the hydrolysis activity of a plurality of multi-region mutants to pNPP at 40 DEG C is improved by nearly 20 times compared with that of a wild type. A lipase Tunnel inlet is modified, so that the hydrolytic activity of the lipase is greatly improved.

The invention discloses an alkaline liquid compound fertilizer as well as a preparation method and application thereof, and belongs to the technical field of fertilizer preparation. The alkaline liquid compound fertilizer comprises the following raw materials: slaughter house leftover slurry, plant ash extract, seaweed extract, potassium humate, a chelated trace element composition, urea formaldehyde, potassium polyphosphate, calcium nitrate, calcium hydroxide, potassium carbonate, a preservative and a synergist. The slaughter house leftover slurry obtained through treatment of specific compound microorganisms and compound enzymatic hydrolysate is adopted as a main active component and is compounded with the synergist ginkgo biloba exocarp extract and other components, various nutrient substances needed by plant growth and development can be generated, and the pH of acid soil is improved. The added compound microorganism bacterial liquid is a strain combination of candida valeri and lactobacillus delbrueckii subspecies bulgaricus, so that the decomposition and liquefaction processes of complex animal organic matters are accelerated, pathogenic bacteria and worm eggs are effectively inhibited, and the safety and stability of the product are remarkably improved.

The invention discloses a method for preparing a zinc-containing polypeptide biocontrol bacterial fertilizer through combination of microorganisms and an enzyme method, and belongs to the technical field of microbial fertilizers. The method specifically comprises the following steps: (1) inoculating a candida utilis strain into a zinc-rich culture medium to obtain a candida utilis culture solution; (2) adding a protease compound into the solution obtained in the step (1) to obtain a zinc-containing polypeptide solution; (3) adding a bacillus thuringiensis mixture into the mixture obtained in the step (2) to obtain zinc-containing polypeptide biocontrol bacterium fermentation liquor, and concentrating the zinc-containing polypeptide biocontrol bacterium fermentation liquor to obtain the zinc-containing polypeptide biocontrol bacterium fertilizer. According to the invention, crops, microbial nutrition, biological control and soil improvement are organically combined, three-in-one functional combination is realized, and compared with a single-function fertilizer, the compound fertilizer has a more remarkable comprehensive effect.

The invention belongs to the technical field of wastewater and sewage treatment, and relates to a preparation method and application of a multi-strain-ceramsite carrier compound for static denitrification purification of aquaculture sewage. The multi-strain-ceramsite carrier compound consists of rhodopseudomonas palustris, pediococcus pentosaceus, bacillus licheniformis, candida albicans, leuconostoc mesenteroides, cellulomonas flavigena and staphylococcus saprophyticus, and is prepared by loading the multi-strain-ceramsite carrier compound on a ceramsite carrier through a special process. When the compound is used for treating aquaculture sewage, strain layered feeding is realized through ceramsite carrier density difference, the aquaculture sewage can be efficiently treated, and odor generation can be remarkably inhibited. Application results show that the total nitrogen degradation rate, the ammonia nitrogen degradation rate and the COD degradation rate of the culture sewage treated by the method are 86.2%, 88.2% and 79.2% respectively. Through the synergistic effect of multiple strains, process innovation and precise spatial distribution control, efficient static purification of the breeding sewage is achieved, and an economical and feasible technical scheme is provided for rural pollution treatment.