39 results about "Candida nivariensis" patented technology

A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 2 and mutated sequences thereof.

The invention relates to a composite microbial preparation for treating a black and odorous river and a preparation method thereof. The composite microbial preparation is made from: Thiobacillus denitrificans powder 40-60 parts, composite Bacillus powder 10-40 parts and composite Saccharomyces cerevisiae powder 10-40 parts. Strains for degrading sulfides and organics in black and odorous sediment and suitable for growing in the black and odorous sediment are specifically screened, the combination of Thiobacillus denitrificans efficient in degrading hydrogen sulfide and removing nitrogen, Bacillus amyloliquefaciens capable of generating rich enzymes such as protease, amylase and cellulase, Saccharomyces cerevisiae, Candida utilis and facultative anaerobic Bacillus licheniformis enables the preparation to quickly improve the water quality of the black and odorous river and remove sediment blackness and odor.

The invention discloses a recombinant pectin methylesterase pmeA, its coding gene and application. The present invention is based on the amino acid sequence of Aspergillus niger pmeA published on GenBank, and then optimizes the codons according to the codon preference of the host bacteria, thereby obtaining a new nucleotide sequence of the pmeA gene such as SEQ ID NO.1 As shown, the amino acid sequence of the expressed recombinant pectin methylesterase is shown in SEQ ID NO.2. After the pmeA gene was connected with the pCuGAPGαAL expression vector, the recombinant plasmid pCuGAPGαAL‑pmeA was integrated into the genome of Candida utilis to realize the heterologous expression of the pmeA gene. The recombinant pectin methylesterase can efficiently catalyze the hydrolysis of methoxy group in pectin, reduce the degree of esterification of pectin, and can be used in the production of food, medicine and chemical industries.

The invention provides a preparation method of a fertilizer making agent. The preparation method includes the following steps that firstly, Cytophaga fermentans ATCC 19072, Cellulomonas flavigena ATCC 482, Butyrivibrio fibrisolvens ATCC 19171 and Clostridium papyrosolvens ATCC 3541 are inoculated to a culture medium with cellulose to be cultured according to the thallus number ratio of 3-17:4-25:5-10:15-28; secondly, bacillus megatherium ACCC 01742, candida tropicalis ACCC 20199 and phanerochaete chrysosporium ACCC 30530 are inoculated to a PDA culture medium to be cultured in a mixed mode according to the thallus number ratio of 5-14:15-20:8-20; thirdly, culture obtained from the first step and the second step is evenly mixed according to the volume ratio of 1:1 and centrifuged, sediment is removed, supernate is taken, and then the fertilizer making agent is obtained. The invention further discloses the fertilizer making agent prepared through the method and application of the fertilizer making agent in preparing edible fungus compost. The fertilizer efficiency of the compost can be remarkably improved, the fertilizer making agent is economical and environmentally friendly, and no influence is generated on growth of crops and soil.

The invention relates to a composite fungicide for treating sludge and a sludge treatment method. The composite fungicide is prepared from the following components in parts by weight: 3 to 8 parts of purple sulfur bacteria, 3 to 8 parts of green sulfur bacteria, 3 to 8 parts of rhodopseudomonas, 3 to 8 parts of rhodopseudomonas capsulata, 3 to 8 parts of rhodococcus rhodochrous, 3 to 8 parts of white-rot fungi, 3 to 8 parts of phosphorus-accumulating bacteria, 3 to 8 parts of anaerobic ammonium oxidation bacteria, 3 to 8 parts of lactobacillus plantarum, 3 to 8 parts of streptomycete, 3 to 8 parts of candida utilis, as well as saccharomyces cerevisiae, lactobacillus lactis, lactobacillus acidophilus, aspergillus niger, aspergillus oryzae, bacillus subtilis and bacillus licheniformis.

The invention relates to a method for producing a high-efficiency biological peptide through enzymolysis and microbe fermentation, which comprises the following steps of: smashing a fermentation substrate, uniformly mixing, then adding water for material blending, and then cooking and sterilizing; when temperature is reduced to 50-55 DEG C, adding a proteolytic enzyme for hydrolyzation; after the hydrolyzation is finished, when the temperature is reduced to 28-35 DEG C, adding a mixed bacterium solution to the hydrolyzed fermentation substrate, uniformity stirring, and carrying out aerobic fermentation under the condition of constant temperature; and carrying out low-temperature drying on the fermented materials, and then smashing to obtain a high-efficiency biological peptide finished product. The fermentation substrate comprises the following materials in parts by weight: 50-60 parts of high-protein bean pulp, 10-20 parts of glutamic acid bacterial protein and 30-40 parts of dephenolized cottonseed protein, and the components are smashed and screened through a 40-mesh screen and then uniformly mixed. The mixed bacterium solution is obtained by mixing the bacterium solutions of aspergillus niger, candida tropicalis, aspergillus oryzae and bacillus subtilis according to the proportion of 1:1:1:1, and the mixed bacterium solution is inoculated into the hydrolyzed fermentation substrate in 5%-10% by weight.