39 results about "Candida nivariensis" patented technology

The subject invention relates to a set of DNA primers which, when utilized in conjunction with the polymerase chain reaction (PCR) assay, can amplify and speciate DNA from five medically important Candida species. Furthermore, the PCR amplified products, generated by the primers, can also be used to create species specific probes which can also detect and confirm the five species of Candida. Thus, the present invention allows for early diagnosis and treatment of an infection. The assay is useful in the context of monitoring antifungal treatment regimens, screening potential antifungal agents, and similar applications requiring quantitative determinations.

A method for treating or preventing infections from yeast of the Candida species is provided wherein an immunoglobulin composition containing high titers of antibodies to staphylococcal adhesins ClfA and SdrG is administered in an amount effective to inhibit the growth and progression of Candidial infections. The compositions and methods of the present invention are advantageous in that they can be used to treat both staphylococcal and Candidial infections at the same time, and they are particularly effective in treating or preventing late-onset sepsis in neonates.

The invention discloses a composite micro-ecological preparation for treating black stink water in rivers, pits and ponds and a preparation method thereof. The composite microbial preparation comprises indigenous microorganism, functional strain and carrier matrix which are separated from a black stink water body to be treated and have a repairing function. The indigenous microorganism is at leastone of nitrifying bacteria, denitrifying bacteria and bacillus subtilis, the functional strain is at least one of photosynthetic bacteria, candida utilis, bacillus megaterium, lactic acid bacteria and actinomycetes, and the carrier matrix is at least one of crocalite, green zeolite, ceramsite, quartz sand, vesuvianite and vermiculite. The black stink water body includes black stink surface waterof black stink rivers, black stink lakes or polluted ground. According to the invention, the composite micro-ecological preparation is formed by immobilizing the indigenous microorganism, the functional strain and the carrier matrix, has remarkable effects of degrading the pollutants such as COD, NH3-N, TP and the like in the black stink water. The preparation method disclosed by the invention haswide applicability to black stink surface water of various black stink rivers, black stink lakes or polluted ground.

The invention relates to a compound microorganism bacterium agent used for sludge reduction, a preparation method and application of the compound microorganism bacterium agent. The compound microorganism bacterium agent is a liquid bacterium agent prepared by fermenting denitrified pseudomonas, nocardia coralline, candida utilis, rhodobacter sphaeroides and aspergillus sojae. According to the invention, the metabiosis action between microbe consortia is utilized to form a biodegradation system in which the activities of various required enzymes are all high, so dead or decrepit thalli in activated sludge can be effectively damaged and decomposed, and meanwhile, organic matters can be decomposed; and the generation quantity of excess sludge is reduced through reducing the quantity of the sludge in the process without influencing the quality of yielding water. On the premise of not greatly changing the traditional sewage treatment process, the sludge reduction ratio can reach 30%-70% in the sewage treatment process by adopting the compound microorganism bacterium agent, and the comprehensive running cost of the sludge treatment can be reduced, so that the compound microorganism bacterium agent is of great significance on the aspects of economic, environmental and social benefits.

The present invention provides a novel dominant selectable marker system in yeast that is based on an aminoglycoside, nourseothricin (NST). This compound possesses a powerful antifungal activity against Candida albicans and S. cerevisiae. The invention provides a cognate drug resistance marker for use in gene transformation and disruption experimentation in Candida albicans and Saccharomyces cerevisiae. In particular, the invention presents: 1) direct utility for gene manipulations in both clinically and experimentally relevant strains regardless of genotype and without affecting growth rate, or hyphal formation; and 2) applicability to antifungal drug discovery, including target validation and various forms of drug screening assays.

The invention discloses a fungus rapid culture, identification and susceptibility detection kit for rapidly and effectively identifying multiple fungi and testing the susceptibility. The invention also discloses a method for directly detecting a clinical sample by using the kit. The kit has the advantages that the multiple fungi are simultaneously identified and different pores are favorable for growing different fungi by coating various sugars or inhibitors on a susceptibility test board, so that the most common fungi in clinic use, such as candida albicans, candida tropicalis, candida glabrata, candida krusei, cryptococcus neoformans, candida guilliermondii and the like, can be identified and effective guidance is provided for the clinical use. The detection method is simple and suitable for detecting pure fungi, can be further directly applied to fungus identification of the clinical sample and the susceptibility detection of antibiotics and has a short detection period, namely thefungi to be detected can be simultaneously identified and the susceptibility can be detected at one time and the result can be given in 24 hours; and thus the detection method greatly shortens the clinical detection period and is favorable for early diagnosis and early therapy of patients.

The invention belongs to the technical field of black and odorous water body treatment, and provides a composite microbial remediation agent for a black and odorous water body and an application thereof. The compound microbial remediation agent for the black and odorous water body is composed of bacillus subtilis, pichia pastoris, candida tropicalis and a nitrifying bacterial agent. The weight ratio of the four bacterial agents is bacillus subtilis:pichia pastoris:candida tropicalis:nitrifying bacterial agent=(3-5):(1-3):(1-2):(1-2). The bacillus subtilis has a specific and efficient degradation effect on organic matters of black and odorous water and is compounded with specific bacterial agents, and the compound microbial remediation agent for the black and odorous water body is obtained.The compound microbial remediation agent is applied to treatment of the black and odorous water body, and has the advantages of all the bacterial agents, and degrades the black and odorous water bodyto the maximum extent. With adopting of the compound microbial remediation agent, after treatment, the odor of the water body is eliminated, the water body is clarified and transparent, and the waterquality COD and the ammonia nitrogen contents meet the V-class standard of 'environmental quality standards for surface water'.

The invention provides a method for identifying candida glabrata by a high-resolution-ratio melting curve, wherein the method includes the following steps: step 1, preparing DNA templates of strains to be identified and a control strain, wherein the strains to be identified includes candida glabrata, candida nivariensis and candida bracarensis; step 2, designing upstream and downstream primers; step 3, obtaining a high-resolution-ratio melting curve chart of each strain by a high-resolution-ratio melting curve technology HRM, and specifically using a Real-time PCR technology, to obtain a high-resolution-ratio dissolution curve chart of each strain through real-time detection of the change of the fluorescence signal value in the DNA melting process; step 4, analyzing and identifying the specific types of the strains to be identified by analysis software; step 5, testing the nucleoside acid sequence of the strain having the result being positive in the step 3; and step 6, verifying the sensitivity of the identification method. The method for identifying candida glabrata by the high-resolution-ratio melting curve can clearly identify different species in the candida glabrata complex,and is simple to operate, accurate and efficient.

The invention relates to an antibody, and a preparation method and application thereof, in particular to a mouse IgG type monoclonal antibody for candida mannan (Mn) antigen, a preparation method of the monoclonal antibody for resisting candida mannan (Mn) antigen, and application of the monoclonal antibody in detection of candida antigen. The invention provides a method which comprises the following steps: breaking candida, then immunizing animals by using the broken candida to prepare hybridoma cells; inoculating the animals by using the hybridoma cells screened by mannan; and separating and purifying to obtain the antibody. The monoclonal antibody prepared by the method has the features of good specificity, high valence and high purity. The monoclonal antibody is used for detecting candida in the field of medical health and scientific research, has the advantages of high sensitivity and good specificity, and is wide in application prospect.

The present invention relates to novel oxodihydropyridinecarbohydrazide derivatives with excellent antifungal activities, an antifungal composition containing the same, and its use for the prevention and treatment of fungal infectious diseases. The oxodihydropyridinecarbohydrazide derivatives of the present invention have excellent antifungal and fungicidal activities, and thus will be useful for the prevention and treatment of various fungal infections by Candida spp., Aspergillus spp., Cryptococcus neoformans and Trichophyton spp., etc. Additionally, the oxodihydropyridinecarbohydrazide derivatives of the present invention, unlike other fungicidal preparations, can be orally administered.

The present invention discloses a fungal DNA extraction method and a two-step PCR amplification technology for detecting clinical fungal infections. The method comprises simple DNA extraction method,design of specific primers for candida albicans, cryptococcus neoformans, and aspergillus fumigatus, and establishment of a detection method for simultaneously detecting the above three fungi. The method is sensitive and specific, and has important significance for clinical diagnosis of three fungal infections.

The invention discloses a candida-killing polypeptide sequence and a preparation method. An amino acid sequence of the candida-killing polypeptide is NH2-RILSILRHQ-COOH. A solid-phase polypeptide synthesis method is adopted for preparation. Defects of proneness to hemolysis and poor biological safety of common antibacterial peptides are overcome. The polypeptide is a novel antifungal polypeptide with a specific candida killing effect and without hemolysis and provides a novel primer for clinical antifungal drug research.

The invention discloses a bactericide for rapidly recovering balanced nutrition of river bottom soil, a preparation method and an application, and belongs to the field of biological agents. The fungicide comprises a microbial agent and a carrier, wherein the microbial agent comprises denitrifying bacteria, nocardia cellulans, lactobacillus plantarum, candida utilis, bacillus subtilis and bacillusmucilaginosus. By simulating the natural environment, screening and optimizing bacterial colony composition and cooperating with a magnetic microbial composite adsorption material, harmful substancesin polluted water are degraded and converted, a microbial ecology structure in a water body is regulated and controlled, growth of indigenous flora is promoted, the effect of purifying water is achieved, growth of aquatic plants is promoted, diseases of aquatic animals are prevented and treated, excessive propagation of algae is inhibited, and finally the effect of stabilizing and repairing the soil ecological environment of the water body is achieved.

The invention provides use of CXCL13 as a biomarker in preparation or screening of diagnostic reagent for monilial infection. The use shows that CXCL13 can be taken as a novel biomarker for diagnosinginvasive monilial infection; and an in-vivo animal experiment shows that the expression level of CXCL13 can be increased by candidas of different strains, and the high expression of CXCL13 is in positive correlation with the seriousness of diseases, so that CXCL13 provided by the invention can be taken as a diagnostic and prognostic indicator for the invasive monilial infection.

A D-arabinitol dehydrogenase enzyme is disclosed. The enzyme is capable of catalyzing the oxidation of D-arabinitol and substantially incapable of catalyzing the oxidation of D-mannitol and is substantially free of other enzymes capable of oxidizing D-mannitol. Also disclosed are methods for determining D-arabinitol. In one embodiment the method comprises the steps of providing in combination (1) a medium suspected of containing D-arabinitol and (2) a D-arabinitol dehydrogenase enzyme and examining the medium for the product of the oxidation of the D-arabinitol. The enzyme utilized is capable of catalyzing the oxidation of D-arabinitol and substantially incapable of catalyzing the oxidation of D-mannitol. Kits for conducting the present method are also disclosed.

The invention relates to the field of biological feeds, and particularly provides a tableted g corn fermented feed for crabs as well as a preparation method and application of the tableted corn fermented feed. The tableted corn fermented feed for crabs comprises a fermentation base material and a composite beneficial bacterium solution, wherein the fermentation base material comprises 100 parts byweight of tableted corn; the composite beneficial bacterium solution is formed by expanding propagation of a composite fermentation strain comprising bacillus subtilis, bacillus coagulans, streptococcus thermophilus, lactobacillus acidophilus, bifidobacterium, pediococcus pentosaceus, candida utilis and saccharomyces cerevisiae; the weight ratio of the composite fermentation strain to the fermentation base material is (0.25-1.0): 1000. The finished product of the tableted corn fermented feed is flaky and is more convenient for the crabs to eat. The crab feed conversion rate can be obviously improved, and the feed coefficient is further reduced; microbial structures of body surfaces and intestinal tracts of the crabs can be improved, food residues are reduced, breeding of harmful microorganisms is reduced, and the survival rate of the crabs is further increased; and the water quality can be improved, water eutrophication is prevented, and a water environment suitable for river crabs iscreated.

The invention relates to the field of biological feeds, and particularly provides a biological fermentation feed for abalones as well as a preparation method and application thereof. The biological fermentation feed for the abalones comprises a fermentation base material and fermentation bacterium fluid; the fermentation base material consists of the following components in parts by weight: 35-40parts of corn, 25-35 parts of soybean meal, 20-40 parts of kelp powder, 10-25 parts of fish meal, 8-12 parts of stone powder, 2-4 parts of calcium dihydrogen phosphate and 0.15-0.25 part of compound enzymes; the fermentation bacterium fluid is produced by expanding propagation of composite bacterial strains including bacillus licheniformis, lactobacillus acidophilus, bifidobacteria, pediococcus pentosaceus, candida utilis and saccharomyces cerevisiae; and the fermentation bacterium fluid and the fermentation base material are mixed in a weight ratio of (0.5-1.0) to 1000. The biological fermentation feed for the abalones provided by the invention can improve survival rate and growth efficiency of abalones, effectively replace antibiotics and reduce drug use, thereby improving quality of theabalones while purifying water and inhibiting vibrio so as to inhibit abalone infection; and thus, the feed is beneficial to ecological healthy culture.

A class of modified salicylaldehyde derivatives has also been synthesized and a series of modified pyridine-based hydrazones identified that have potent antimicrobial activity against multiple Candida spp. These compounds have been characterized using fungal growth inhibition assays, mammalian cell toxicity assays, time-kill assays and synergy studies of these novel pyridine-based hydrazones on both azole-susceptible and azole-resistant fungal species. Effectiveness of these compounds in inhibiting the growth of protozoal parasites was also found.

The invention discloses a composite microorganism water purification agent for purifying a sea-farming water body. The composite microorganism water purification agent comprises composite bacterial liquid, wherein the composite bacterial liquid is prepared by mixing bacillus subtilis with bacillus licheniformis, bacillus coagulans, pseudomonas fluorescens, rhodotorula benthica, candida utilis, aspergillus niger, rhizopus oryzae, plant bacterium lacticum, lactobacillus acidophilus, marine photosynthetic bacteria and the like. The composite microorganism water purification agent can solve the problems that in the prior art, the quantity of bacterial strains contained in the microorganism water purification agent is low, the viability is poor, the microorganism water purification agent is difficult for field planting in the water body, so that the kind and the quantity of the bacterial strains in the water quality purification agent can be increased, the activity of the bacterial strainscan be increased, the field planting capacity of the bacterial strains in the water body can be improved, and deep-level comprehensive purification of nitrification and denitrification for water quality can be realized.