33 results about "Malassezia" patented technology

The invention relates to a sodium hyaluronate fermentation filtrate as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. Through a two-stage fermentation mode, the fermentation filtrate containing sodium hyaluronate is prepared by recombining bacillus subtilis, and then residual nutrient substances in a culture medium are consumed by inoculating bacillus velezensis, so that the nutrient substances are metabolized into active substances with repairing and strengthening effects on hair; the prepared sodium hyaluronate fermentation filtrate has the effects of repairing scalp and strengthening hair. The sodium hyaluronate filtrate disclosed by the invention can be used for well reducing the content of NO in macrophages (the NO content of cells is increased due to malassezia lysate), can be used for removing active oxygen ROS of HaCaT cells and increasing the total tensile strength of hair, and has the potential effects of relieving scalp, resisting oxidative stress and enhancing the toughness of the hair.

The present application relates to a kind of plant anti-dandruff composition with oil control and bacteriostasis efficacy and application thereof, belong to the field of cosmetics.The composition includes soapberry fruit extract, camellia seed extract, artemisia annua extract, valerian root extract, phyllanthus emblica fruit extract;The valerian root extract is extracted after being inoculated with mixed inoculant consisting of bacillus licheniformis and lactobacillus bulgaricus and then fermented with valerian root as raw material.The valerian root extract obtained by the fermentation of specific strains and strain ratio mixture is significantly improved in the bacteriostatic effect on malassezia and propionibacterium acnes.Further, the present application selects specific soapberry fruit extract, camellia seed extract, artemisia annua extract, phyllanthus emblica fruit extract and the obtained valerian root extract within a specific proportion range, and the obtained composition has synergistic effect of each component, which can significantly improve its oil control, bacteriostasis and anti-dandruff effect.

This invention provides a plant composition, its preparation method, and its application, relating to the field of plant essential oil technology. The plant essential oils extracted from Alpinia oxyphylla, Agrimonia pilosa, coriander seeds, and Hibiscus syriacus bark have excellent inhibitory effects against Malassezia; they are also non-irritating to human skin and can be used in daily chemical hair care product formulations to effectively inhibit dandruff.

This invention discloses a targeted antibacterial and repairing scalp shampoo containing a four-herb compound and its segmented preparation process, belonging to the technical field of functional herbal hair care products. Based on the traditional Chinese medicine theory of principal, assistant, and adjuvant herbs, this invention uses a precise compound of four herbs—Platycladus orientalis leaf, Clematis chinensis, Dictamnus dasycarpus bark, and Paeonia suffruticosa—combined with an amino acid cleansing system and multiple antibacterial and repairing components to create a synergistic formula. It employs a unique segmented salt-addition and low-temperature step-by-step feeding process, locking in the activity of the herbs at 35℃~40℃. Stability is regulated through two salt additions, and properties are optimized through constant-temperature maturation, solving the problems of traditional products such as activity loss, poor stability, single efficacy, and disordered formulation. This product can target and inhibit Malassezia, eradicate stubborn dandruff, reduce inflammation and itching, balance oil and water, repair the scalp barrier, and nourish hair roots. Its pH is suitable for the slightly acidic environment of the scalp, making it gentle and non-irritating, suitable for long-term treatment of sensitive, seborrheic, and permed / dyed scalps. The formula and process are novel, creative, and practical, and can be industrially mass-produced.

The invention belongs to the technical field of traditional Chinese medicine preparations, and particularly relates to Chinese herbal medicine emulsifiable paste for dermatitis and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following raw materials: radix sophorae flavescentis, cortex phellodendri, cortex dictamni, fructus cnidii, fructus kochiae, radix scutellariae, radix paeoniae rubra, radix salviae miltiorrhizae, herba schizonepetae, radix saposhnikoviae Systematic design is carried out aiming at complex pathogenesis of seborrheic dermatitis, medicinal materials such as radix sophorae flavescentis and cortex phellodendri are used for synergistically clearing heat and drying dampness, malassezia is inhibited, and lipid control and bacteriostasis are carried out from the source; radix scutellariae, radix salviae miltiorrhizae and the like are combined to cool and activate blood and control inflammation for a long time; the herba schizonepetae, the radix saposhnikoviae and the like are added for targeted itching relieving and secondary injury reduction; in the dosage form, a low-oil-burden matrix composed of light grease such as jojoba oil and squalane is adopted, the texture is light and thin, hair follicles are not blocked, and phytosterol is introduced to synchronously repair the skin barrier; according to the system, the use comfort and compliance are remarkably improved while permeation of active ingredients is guaranteed.

The invention provides a traditional Chinese medicine composition for regulating scalp micro-ecology, an external preparation containing the traditional Chinese medicine composition and application of the external preparation, and relates to the technical field of traditional Chinese medicine preparations. The traditional Chinese medicine composition for regulating and controlling scalp micro-ecology is prepared from cacumen biotae, rhizoma cyperi, fructus cnidii, cortex dictamni, radix sanguisorbae, herba ecliptae and herba menthae. Based on the compatibility principle of monarch, minister, assistant and guide, the composition takes fructus cnidii for eliminating dampness and killing parasites as monarch, cortex dictamni and cacumen biotae for removing heat to cool blood as minister, garden burnet, eclipta and rhizoma cyperi for astringing, detoxifying, nourishing yin and regulating qi as assistant, and mint for guiding the medicine to move upwards and transdermal permeation as guide, so that multiple effects of inhibiting bacteria, resisting inflammation, repairing a barrier, regulating sebum secretion and the like are synergistically realized. Modern pharmacological studies show that the active ingredients can inhibit malassezia proliferation, regulate and control inflammatory pathways of NF-kB, TLR4 and the like, reduce expression of proinflammatory factors of IL-6, TNF-alpha and the like, and promote skin barrier repair. Clinical observation shows that the composition can significantly improve the scalp seborrheic dermatitis symptom.

The invention discloses a composition of antibacterial peptide and a plant extract. The composition is prepared from epsilon-polylysine, ginseng root water and raspberry fruit water. Compared with a single component, the composition of the antibacterial peptide and the plant extract has the advantages that the antibacterial and bactericidal effects are obviously improved, and the composition has an obvious antibacterial effect on propionibacterium and malassezia, and has an easy-to-comb function when being used in hair washing products. The composition has good effects of resisting bacteria, removing acnes and removing dandruff, is soft and easy to comb, and has storage stability.

The invention relates to the technical field of medicines, and particularly discloses a polypeptide Loni-P extracted from traditional Chinese medicine honeysuckle, and the amino acid sequence of the polypeptide Loni-P is SEQ ID No.1. The polypeptide can be prepared from honeysuckle through the steps of ultrasonication, enzymolysis, purification and the like. Experiments show that the inhibition rate of Loni-P to malassezia under the concentration of 2 mu g / mL reaches 86 + / -5.29%, which is similar to that of positive control ketoconazole (92 + / -9.17%). The invention further provides a medicated bath component containing Loni-P. The medicated bath component is composed of polypeptide, beta-cyclodextrin, chitosan, fumaric acid, sodium hydroxide and other auxiliary materials and is matched with 200 L of medicated bath water. Clinical experiments show that the medicated bath can remarkably relieve itching, skin lesion area and shape of a chronic eczema patient, and after 30 days of treatment, the score of an experimental group is reduced from 7.93 to 4.0 (a control group has no remarkable change). The polypeptide is natural in source and high in safety, and a new strategy is provided for eczema treatment.

The invention discloses oil-controlling and dandruff-removing shampoo and a preparation method thereof. The shampoo comprises an anti-malassezia single-domain antibody with an amino acid sequence as shown in SEQ ID NO: 1 and an asparagus cochinchinensis root extract obtained through supercritical carbon dioxide extraction. The activity retention rate of the antibody is not lower than 80% after the antibody is stored in a shampoo matrix at 40 DEG C for 28 days. The total polyphenol content of the extract is not less than 5%. Chessboard microdilution determination shows that the antibody and the extract have a synergistic effect in the aspect of inhibiting malassezia, and the synergistic index is less than 0.9. Cell experiments show that the extract can down-regulate gene expression of 5 alpha-reductase in human sebaceous gland cells. A human body test result shows that after the shampoo is used for 4 weeks, the dandruff score of a subject is reduced by 82.5%, the scalp grease content is reduced by 64.1%, the malassezia quantity is reduced by 47.8%, and the effect is superior to that of a single component and a commercially available chemical dandruff removing product. The shampoo has excellent stability, synergistic effect and clinical application effect, and has a relatively high industrial application prospect.

The invention discloses long-acting anti-dandruff shampoo based on microbial balance regulation and a preparation method of the long-acting anti-dandruff shampoo. The shampoo comprises a cleaning system composed of sodium lauroyl sarcosinate, cocamidopropyl betaine and decyl glucoside, a micro-ecological regulation system composed of piroctone olamine, inulin and a lactobacillus fermentation product, and skin barrier repairing lipids such as ceramide and squalane. According to the preparation method, all the components are effectively integrated through a step-by-step temperature control and homogeneous emulsification process. Through the synergistic effect of the components, malassezia is efficiently inhibited, effective microbial communities on scalp are selectively maintained, and a skin barrier is repaired, so that scalp micro-ecological balance is regulated and controlled, long-acting dandruff removal is realized, the scalp health level is improved, and the scalp care effect is improved. The problems that a traditional anti-dandruff product is likely to cause dysbacteriosis, the effect is not lasting, and relapse is caused after the traditional anti-dandruff product is stopped are solved.

The present application relates to the technical field of scalp care, and specifically provides a dandruff-removing composition containing sophora flavescens polypeptide, a preparation method and application thereof.The dandruff-removing composition contains various active ingredients such as sophora flavescens polypeptide SoFla-I, salicylic acid, selenium disulfide, glutathione and nicotinamide, and is prepared through a specific process.The amino acid sequence of the sophora flavescens polypeptide SoFla-I is shown as SEQ ID No.1, and the preparation includes steps such as freeze-drying of sophora flavescens roots, ultrasonic extraction, ammonium sulfate precipitation and dialysis purification.Experiments show that the composition can effectively inhibit malassezia, and significantly improve dandruff and pruritus symptoms, and the dandruff score and pruritus score significantly decrease after 14 days of use, and the satisfaction of the subjects is high.The composition of the present application is high in safety and significant in dandruff-removing effect, and is suitable for shampoo products, scalp care agents and the like.

The invention discloses a preparation method of a monoclonal antibody for detecting trichophyton rubrum and application of the monoclonal antibody in fungal skin disease detection, and belongs to the field of biological medicine. The monoclonal antibody provided by the invention only has specific reaction with trichophyton rubrum, and has no cross reaction with common fungi such as trichophyton mentagrophytes, epidermophyton flocculent, candida albicans, malassezia and the like and normal flora of a human body, so that a false positive result is effectively avoided. Based on the double-antibody sandwich principle, the detection sensitivity can reach 200 pg / mL level, trace trichophyton rubrum in a clinical sample can be detected, and the positive rate is obviously higher than that of direct microscopic examination. The whole detection process can be completed within 15-20 min and is far faster than fungus culture for several weeks, a rapid diagnosis basis can be provided for doctors, and early treatment is facilitated. The kit provided by the invention has standardized operation steps, does not need complex instruments and special training, and is suitable for popularization and use in clinical laboratories of hospitals at all levels, outpatient clinics of dermatology and even basic medical institutions.

This invention discloses a composition with hair-care effects on damaged hair and its application, comprising *Ulva prolifera* extract, *Angelica sinensis* extract, *Lichen davidii* extract, and *Camellia chrysantha* extract, wherein the weight ratio of the *Ulva prolifera* extract, *Angelica sinensis* extract, *Lichen davidii* extract, and *Camellia chrysantha* extract is (1-3):(1-2):(3-7):(5-8). It has the effects of repairing hair cuticles, nourishing hair, improving hair shine, improving combability, scavenging free radicals, and inhibiting Malassezia.

The invention discloses a plant composition for regulating scalp micro-ecology and application thereof, and relates to the technical field of cosmetics. The invention provides a plant composition which is prepared from magnolia bark and uncaria rhynchophylla, and the feeding ratio of the magnolia bark to the uncaria rhynchophylla is 7: 3-3: 7. The plant composition has the effect of synergistically promoting expression of scalp keratinocyte human beta-defensin, has an inhibiting effect on malassezia and does not have an inhibiting effect on staphylococcus epidermidis under the concentration of 0.5%-2%, has the effect of adjusting scalp flora balance, and can relieve the degree of scalp itching and reduce the number of scurf.

This application provides the use of an antimicrobial peptide in the preparation of skin care products. The antimicrobial peptide of this application can effectively inhibit *Propionibacterium acnes* and *Malassezia*, thereby preventing skin problems such as acne, skin inflammation, and dandruff caused by the overgrowth of these two bacteria. Therefore, the antimicrobial peptide of this application has skin care uses in inhibiting acne, relieving skin inflammation such as folliculitis, pityriasis versicolor, and seborrheic dermatitis, and in inhibiting dandruff, and has significant practical value.

This invention discloses the application of an artemisia annua compound as the sole active ingredient in the preparation of topical formulations for the prevention or improvement of blackheads and acne. Based on 100% of the total mass of the topical formulation, the artemisia annua compound accounts for 0.1% to 0.22%, and is composed of the following raw materials in parts by mass: camphene 4 to 10 parts, artemisinin 1 part. This invention also discloses a topical formulation for the prevention or improvement of blackheads and acne, the sole active ingredient of which is an artemisia annua compound. Based on 100% of the total mass of the topical formulation, the artemisia annua compound accounts for 0.1% to 0.22%, and is composed of the following raw materials in parts by mass: camphene 4 to 10 parts, artemisinin 1 part. The artemisia annua compound of this invention can significantly reduce the formation of squalene peroxide in a UVA-induced squalene photo-oxidation model, and shows stronger inhibition against Malassezia and less impact on Staphylococcus epidermidis in a Malassezia vs. symbiotic bacteria comparison model.

This invention addresses the prevalent hair loss problem in modern people by providing a pharmaceutical composition comprising Sanguisorba officinalis extract and Sophora flavescens extract. Topical application improves hair growth in a hair loss model mouse, resulting in thicker, shinier hair. Both hair weight and length show significant improvement compared to the model group, the Sanguisorba officinalis-only group, and the Sophora flavescens-only group, indicating synergistic effects. This may be related to its inhibition of Malassezia scalp, thereby improving the scalp environment. In particular, the co-decoction extract of Sanguisorba officinalis and Sophora flavescens shows superior efficacy compared to physically mixed Sanguisorba officinalis and Sophora flavescens extracts in the same proportion. Optimal efficacy was achieved at a Sanguisorba officinalis:Sophora flavescens weight ratio of 2:8, demonstrating the therapeutic advantage of the co-decoction extract. In volunteer use cases, the co-decoction extract showed similar excellent effects in improving scalp sebum secretion and narrowing the hair loss area, as well as a significant clearing effect on folliculitis.

The present application provides a kind of emulsion for improving pet skin problems and its preparation method, it is related to the technical field of traditional Chinese veterinary medicine pharmacy.The emulsion is prepared by emulsification homogenization from traditional Chinese medicine extract, traditional Chinese medicine essential oil, plant fatty acid, emulsifier and water, wherein the traditional Chinese medicine extract is Astragalus and ginseng extract, and the traditional Chinese medicine essential oil is a compound of clove basil oil, angelica oil, rose oil and kempas seed oil.The present application solves the problems of large irritability, easy drug resistance of chemical drugs and poor absorption of traditional Chinese medicine by synergistic compounding of active ingredients.The product has the functions of antibacterial, barrier repair, sebum regulation and moisturizing, and the inhibition zone diameters of staphylococcus aureus and malassezia are 12.5±0.5 mm and 10.2±0.3 mm respectively, and it can significantly improve skin moisturizing function, and is mild and non-irritating, suitable for the treatment and care of various skin problems of pets.

The present application relates to the technical field of antibacterial peptides, in particular to an antibacterial peptide for inhibiting Malassezia, a preparation method and application thereof. The antibacterial peptide for inhibiting Malassezia comprises any one of the following (a)-(d) polypeptides: (a) a polypeptide comprising an amino acid sequence shown in SEQ ID NO. 1, or a polypeptide consisting of amino acid residues shown in SEQ ID NO. 1; (b) a polypeptide formed by substitution, deletion and / or addition of one or more amino acid residues in the amino acid sequence defined in (a); (c) a polypeptide obtained by modifying the N-terminal and / or C-terminal of the polypeptide defined in (a); and (d) a polypeptide having 99% or more, 95% or more, 90% or more, 85% or more, or 80% or more homology with the amino acid sequence defined in (a). The antibacterial peptide for inhibiting Malassezia has good safety to the skin, and can be used for treating dandruff, seborrheic dermatitis, tinea versicolor and Malassezia folliculitis caused by Malassezia fungal infection, and can be applied to cosmetics or external-use drugs for treating dandruff, seborrheic dermatitis, tinea versicolor and Malassezia folliculitis. The antibacterial peptide has significant antibacterial effect on Malassezia restricta and Malassezia furfur, and has great application value.

The application discloses an effect of 4-HIL in promoting hair follicle health, inhibiting androgenetic alopecia and dandruff generation and application thereof, and belongs to the technical field of application of non-protein amino acids. It is found for the first time that 4-HIL has the effects of promoting hair follicle proliferation, reducing hair follicle apoptosis, increasing the number of hair follicles in a growth period and the total number of hair follicles; promoting the growth speed of androgenetic alopecia scalp hair, increasing hair length and hair weight; and inhibiting the effect of keratinocyte apoptosis caused by Malassezia. 4-HIL can be used for preparing a medicament for inhibiting androgenetic alopecia, inhibiting dandruff generation and maintaining the protection function of the stratum corneum and / or a hair washing and caring product. The 4-HIL has the advantages of good effect, high safety, good biological activity, wide application range and low price, and has a great application prospect in the preparation of products for promoting hair follicle health, inhibiting androgenetic alopecia and dandruff generation.

The invention belongs to the technical field of biological medicines, and particularly discloses a polypeptide with malassezia resisting activity and application of the polypeptide in improvement of seborrheic dermatitis. The polypeptide provided by the invention comprises an amino acid sequence as shown in SEQ ID NO: 1. The polypeptide has a remarkable inhibiting effect on the key pathogenic bacterium-malassezia causing seborrheic dermatitis, experimental data shows that the polypeptide can remarkably inhibit the growth and constant value of the malassezia at a low concentration, and the effect of the polypeptide can be close to that of a positive control ketoconazole. The polypeptide shows an excellent dermatophyte removing effect in a malassezia induced mouse seborrheic dermatitis model, experimental data proves that the fungal load of the skin tissue of the affected part of a mouse is remarkably reduced after administration, and the polypeptide still can keep high biological activity and stability in a complex skin physiological microenvironment, and has a good application prospect. The malassezia can effectively penetrate through a sebum layer to act on thalli, so that the decomposition effect of the malassezia on sebum is effectively restrained.

The present invention primarily relates to compositions comprising piroctone olamine and at least one 1,2-alkanediol for use in the treatment of an excess of Malassezia and / or Staphylococcus on the skin surface of mammals, preferably of humans. The present invention further relates to products, and to the cosmetic, non-therapeutic use of a composition comprising a particular amount of piroctone olamine and 1,2-alkanediol or of products comprising such compositions to reduce the amount of Malassezia and / or Staphylococcus on the skin surface of mammals, preferably of humans.

The present application provides a hair lotion for nourishing hair roots and scalp care and a preparation method thereof, which comprises a hair root nourishing system composed of keratin and hexamidine diisethionate, a scalp soothing system composed of extracts of Centella asiatica and giant knotweed in a specific ratio, and a mild cleaning system composed of an amino acid, a zwitterionic surfactant and a non-ionic surfactant. The product is prepared by low-temperature emulsification, step-by-step addition of active substances and aging process. Through the synergistic effect of multiple components, the product can effectively promote hair follicle cell proliferation, inhibit inflammatory factors and Malassezia, and has multiple functions such as cleaning, nourishing, soothing and strengthening hair, etc., solving the problems of single function, strong irritation and easy inactivation of active substances of traditional products.

The application discloses a kind of nanometer composition for scalp deep anti-inflammatory and improving hair follicle environment and its preparation method and application.The composition is made of nanometer ascorbic acid palmitate 5-10 parts, liposome VC 3-8 parts, menthol 15-25 parts, collagen 5-10 parts, vitamin B6 2-4 parts, zinc 0.5-1 part.The application significantly enhances the transdermal penetration of active ingredients by nanotechnology;Synergistic effect of each component can effectively inhibit the growth of malassezia, reduce the expression of inflammatory factors IL-6 and TNF-α, promote the production of type I collagen, realize scalp deep anti-inflammatory and hair follicle repair.Experiments show that the bacteriostatic effect of the composition is significantly better than that of commercially available products, and the anti-inflammatory and repair effect is equivalent to that of positive control, and the total effective rate reaches 88.3%.The application is safe and non-irritating, and is suitable for hair care products such as shampoo and hair conditioner.

The invention discloses menthol citrate as well as a preparation method and application thereof. In particular discloses menthol citrate and application thereof in inhibition of malassezia, staphylococcus aureus, escherichia coli and / or candida albicans. The menthol citrate has good malassezia resisting activity, has a short-time (1-3 minutes) efficient inhibition effect on malassezia, can efficiently inhibit staphylococcus aureus, escherichia coli and candida albicans at the same time, can achieve broad-spectrum bacteriostasis, and has potential application value in scalp micro-ecological management. The menthol citrate provided by the invention is an ester compound with lipophilic and hydrophilic balance characteristics, has a cool skin feeling and better formula adaptability, and can be widely applied to cosmetics, wash supplies, skin care products, scalp care products, hair care products and scalp micro-ecological care products; comprising products with anti-dandruff activity and a scalp micro-ecological restoration function.

The scalp micro-ecological balance fluffy shampoo comprises the following components in percentage by weight: 3%-8% of a lactobacillus material, 1%-3% of prebiotics, 0.05%-0.2% of natural antibacterial peptide, 1.0%-1.5% of a wild kudzuvine root extract, 1.1%-1.3% of a fructus forsythiae fruit extract, 0.6%-0.9% of a radix gentianae extract, 0.8%-1.2% of a rhizoma anemarrhenae root extract, 12%-18% of an amino acid surfactant, 0.3%-0.7% of a pH regulator and the balance of deionized water. The natural antibacterial peptide and four plant extracts (pueraria mirifica, fructus forsythiae fruit, radix gentianae and rhizoma anemarrhenae root) form a composite antibacterial system, active ingredients reserved by supercritical CO2 extraction and nisin are in synergistic effect, the maximum malassezia inhibition rate can reach 94.1%, and the problems of scurf and itching are effectively solved.

The application discloses a bacteriostatic, itching-relieving and antioxidant traditional Chinese medicine composition taking a prepared radix polygoni multiflori extract as a main active ingredient, which is composed of the following ingredients in parts by weight: the prepared radix polygoni multiflori extract 15-35 parts, the cypress leaf extract 5-20 parts, the black sesame seed extract 3-12 parts and the ligustrum lucidum fruit extract 3-12 parts. The ultrasonic-assisted cellulase extraction is adopted to greatly improve the dissolution rate of stilbene glucoside in the prepared radix polygoni multiflori and the active ingredient content of the extract is high; the multi-flavor traditional Chinese medicine is compounded to realize the bacteriostasis (staphylococcus aureus and malassezia), itching relief and antioxidant effects in a synergistic manner, the bacteriostasis circle diameter is greater than or equal to 15 mm, and the DPPH free radical scavenging rate is greater than or equal to 75%; the natural traditional Chinese medicine ingredient is mild and non-irritating, is suitable for a scalp care scene, and can be widely used in hair washing and hair care daily chemical products.

The invention relates to a soothing and bacteriostatic composition and application thereof in shower gel, and belongs to the technical field of cosmetics. The soothing and bacteriostatic composition disclosed by the invention comprises a fermentation substrate and a fermentation strain, wherein the fermentation substrate comprises a sargassum extract, an aesculus chinensis extract and a hemerocallis fulva flower extract; the mass ratio of the sargassum extract to the aesculus chinensis extract to the hemerocallis fulva flower extract in the fermentation substrate is 1: (0.1-0.7): (3-6); the fermentation strain comprises bailey sporospora. The soothing and bacteriostatic composition prepared from the fermentation substrate and the fermentation strain limited by the invention has the advantages of remarkably inhibiting the activity of hyaluronidase and inhibiting the activity of malassezia and propionibacterium acnes, and the soothing and bacteriostatic effects of the shower gel can be remarkably improved when the soothing and bacteriostatic composition is applied to the shower gel.

The invention relates to a graded preparation method of bacteriostatic active components based on multi-color mango peel residues, which comprises the following steps: crushing dried mango peel residues, and carrying out enzymolysis pretreatment by using a compound enzyme system containing cellulase and pectinase; carrying out ultrasonic-assisted extraction by adopting a deep eutectic solvent in which the molar ratio of choline chloride to glycerol is 1: 2, and carrying out centrifugal separation to obtain a crude extract; loading the crude extract on a macroporous adsorption resin column, carrying out gradient elution by using an ethanol-water solution containing 1-3% of hydroxypropyl-beta-cyclodextrin as a protective eluent, and collecting an active fraction; and directly cutting the active fraction into a preparative high performance liquid chromatography system by adopting a column switching technology, collecting a chromatographic peak with the retention time of 14.5-15.5 minutes, and volatilizing a solvent to obtain the targeted malassezia-resistant active component. The method can be used for preparing medicines or cosmetics for inhibiting malassezia.

The invention discloses a primer group for simultaneously detecting eight pet dermatophytes and application of the primer group. The primer group comprises a forward PCR (Polymerase Chain Reaction) amplification primer, a reverse PCR amplification primer and a control primer sequence and a template of eight pet skin fungi, wherein the eight pet skin fungi comprise microsporum canine, microsporum gypsiformis, malassezia, megasporum, trichophyton mentagrophytes, trichophyton fibraureae, histoplasma capsulatum and globularia; the sequences of the primers are respectively shown as SEQ ID NO.1-SEQ ID NO.19. The primers can detect eight pet dermatomycosis, have very high sensitivity and specificity, have certain development, utilization and popularization values, and can provide technical support for clinical diagnosis of pet dermatomycosis.