61 results about "Malassezia" patented technology

The invention relates to a Paenibacillus polymyxa strain B01 and application of the Paenibacillus polymyxa strain B01, and belongs to the technical field of microorganisms. The paenibacillus polymyxa B01 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the paenibacillus polymyxa B01 is GDMCC NO.66594. The strain is obtained by separation, purification and screening from a soil sample, and tests show that the strain has excellent pH and high temperature tolerance; antibacterial active substances generated by fermentation of the bacillus subtilis have a relatively good antibacterial effect on escherichia coli, pseudomonas aeruginosa, pseudomonas putida, staphylococcus aureus, candida albicans, aspergillus brazilis, propionibacterium acnes and malassezia; and the antibacterial active substance has excellent heat resistance, cold resistance, illumination stability, pH stability and preservative effect, belongs to a mild low-irritation raw material, can be used for preparing daily chemical products and medicines, and has wide application prospects and important conversion research values.

The invention relates to a sodium hyaluronate fermentation filtrate as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. Through a two-stage fermentation mode, the fermentation filtrate containing sodium hyaluronate is prepared by recombining bacillus subtilis, and then residual nutrient substances in a culture medium are consumed by inoculating bacillus velezensis, so that the nutrient substances are metabolized into active substances with repairing and strengthening effects on hair; the prepared sodium hyaluronate fermentation filtrate has the effects of repairing scalp and strengthening hair. The sodium hyaluronate filtrate disclosed by the invention can be used for well reducing the content of NO in macrophages (the NO content of cells is increased due to malassezia lysate), can be used for removing active oxygen ROS of HaCaT cells and increasing the total tensile strength of hair, and has the potential effects of relieving scalp, resisting oxidative stress and enhancing the toughness of the hair.

The invention discloses radix notoginseng stem and leaf rare ginsenoside as well as a preparation method and application thereof, and belongs to the technical field of ginsenoside. The radix notoginseng stem and leaf rare ginsenoside comprises Rk3, Rh4, S-Rg3, R-Rg3, Rk1 and Rg5. The radix notoginseng stem and leaf rare ginsenoside can be used for preparing radix notoginseng stem and leaf rare ginsenoside. The preparation method comprises the following steps: mixing the pseudo-ginseng stem and leaf extract with an acetic acid aqueous solution, reacting at 105-120 DEG C for 10-40 minutes, and drying; in the preparation process, macroporous resin purification is not carried out. According to the scheme, part of chemical substances in the panax notoginseng stem and leaf extract can be rapidly converted at a low temperature, new active substances Rh4, S-Rg3, R-Rg3, Rk1 and Rg5 with high purity are obtained at the same time, and the obtained panax notoginseng stem and leaf rare ginsenoside can effectively inhibit propionibacterium acnes, burkholderia cepacia and malassezia furfur.

The present application relates to a kind of plant anti-dandruff composition with oil control and bacteriostasis efficacy and application thereof, belong to the field of cosmetics.The composition includes soapberry fruit extract, camellia seed extract, artemisia annua extract, valerian root extract, phyllanthus emblica fruit extract;The valerian root extract is extracted after being inoculated with mixed inoculant consisting of bacillus licheniformis and lactobacillus bulgaricus and then fermented with valerian root as raw material.The valerian root extract obtained by the fermentation of specific strains and strain ratio mixture is significantly improved in the bacteriostatic effect on malassezia and propionibacterium acnes.Further, the present application selects specific soapberry fruit extract, camellia seed extract, artemisia annua extract, phyllanthus emblica fruit extract and the obtained valerian root extract within a specific proportion range, and the obtained composition has synergistic effect of each component, which can significantly improve its oil control, bacteriostasis and anti-dandruff effect.

This invention provides a plant composition, its preparation method, and its application, relating to the field of plant essential oil technology. The plant essential oils extracted from Alpinia oxyphylla, Agrimonia pilosa, coriander seeds, and Hibiscus syriacus bark have excellent inhibitory effects against Malassezia; they are also non-irritating to human skin and can be used in daily chemical hair care product formulations to effectively inhibit dandruff.

This invention discloses a targeted antibacterial and repairing scalp shampoo containing a four-herb compound and its segmented preparation process, belonging to the technical field of functional herbal hair care products. Based on the traditional Chinese medicine theory of principal, assistant, and adjuvant herbs, this invention uses a precise compound of four herbs—Platycladus orientalis leaf, Clematis chinensis, Dictamnus dasycarpus bark, and Paeonia suffruticosa—combined with an amino acid cleansing system and multiple antibacterial and repairing components to create a synergistic formula. It employs a unique segmented salt-addition and low-temperature step-by-step feeding process, locking in the activity of the herbs at 35℃~40℃. Stability is regulated through two salt additions, and properties are optimized through constant-temperature maturation, solving the problems of traditional products such as activity loss, poor stability, single efficacy, and disordered formulation. This product can target and inhibit Malassezia, eradicate stubborn dandruff, reduce inflammation and itching, balance oil and water, repair the scalp barrier, and nourish hair roots. Its pH is suitable for the slightly acidic environment of the scalp, making it gentle and non-irritating, suitable for long-term treatment of sensitive, seborrheic, and permed / dyed scalps. The formula and process are novel, creative, and practical, and can be industrially mass-produced.

The invention belongs to the technical field of traditional Chinese medicine preparations, and particularly relates to Chinese herbal medicine emulsifiable paste for dermatitis and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following raw materials: radix sophorae flavescentis, cortex phellodendri, cortex dictamni, fructus cnidii, fructus kochiae, radix scutellariae, radix paeoniae rubra, radix salviae miltiorrhizae, herba schizonepetae, radix saposhnikoviae Systematic design is carried out aiming at complex pathogenesis of seborrheic dermatitis, medicinal materials such as radix sophorae flavescentis and cortex phellodendri are used for synergistically clearing heat and drying dampness, malassezia is inhibited, and lipid control and bacteriostasis are carried out from the source; radix scutellariae, radix salviae miltiorrhizae and the like are combined to cool and activate blood and control inflammation for a long time; the herba schizonepetae, the radix saposhnikoviae and the like are added for targeted itching relieving and secondary injury reduction; in the dosage form, a low-oil-burden matrix composed of light grease such as jojoba oil and squalane is adopted, the texture is light and thin, hair follicles are not blocked, and phytosterol is introduced to synchronously repair the skin barrier; according to the system, the use comfort and compliance are remarkably improved while permeation of active ingredients is guaranteed.

The invention relates to a traditional Chinese medicine external preparation for treating scalp inflammation. The traditional Chinese medicine external preparation is prepared from honeysuckle, radix sophorae flavescentis, fructus kochiae, cortex dictamni, dandelion, radix salviae miltiorrhizae, menthol, borneol and a transdermal enhancer. The preparation plays anti-inflammatory, bacteriostatic and barrier repairing roles through a multi-target synergistic mechanism, and is suitable for scalp inflammatory diseases such as seborrheic dermatitis and contact dermatitis. The preparation maximally retains active ingredients by adopting a specific extraction process, and the transdermal efficiency is improved by combining a triple permeation enhancing technology. The active ingredients reach the treatment concentration in the deep hair follicle. The pH value (5.5-6.0) of the preparation is matched with the weak acid environment of scalp, malassezia is selectively inhibited without damaging effective microbial communities, and the recurrence risk is reduced from the source. A spray, a lotion and a gel are proved to be capable of remarkably relieving erythema pruritus, inhibiting pathogenic bacteria colonization and accelerating cuticle repair, and have no skin irritation.

The invention provides a traditional Chinese medicine composition for regulating scalp micro-ecology, an external preparation containing the traditional Chinese medicine composition and application of the external preparation, and relates to the technical field of traditional Chinese medicine preparations. The traditional Chinese medicine composition for regulating and controlling scalp micro-ecology is prepared from cacumen biotae, rhizoma cyperi, fructus cnidii, cortex dictamni, radix sanguisorbae, herba ecliptae and herba menthae. Based on the compatibility principle of monarch, minister, assistant and guide, the composition takes fructus cnidii for eliminating dampness and killing parasites as monarch, cortex dictamni and cacumen biotae for removing heat to cool blood as minister, garden burnet, eclipta and rhizoma cyperi for astringing, detoxifying, nourishing yin and regulating qi as assistant, and mint for guiding the medicine to move upwards and transdermal permeation as guide, so that multiple effects of inhibiting bacteria, resisting inflammation, repairing a barrier, regulating sebum secretion and the like are synergistically realized. Modern pharmacological studies show that the active ingredients can inhibit malassezia proliferation, regulate and control inflammatory pathways of NF-kB, TLR4 and the like, reduce expression of proinflammatory factors of IL-6, TNF-alpha and the like, and promote skin barrier repair. Clinical observation shows that the composition can significantly improve the scalp seborrheic dermatitis symptom.

The invention discloses a composition of antibacterial peptide and a plant extract. The composition is prepared from epsilon-polylysine, ginseng root water and raspberry fruit water. Compared with a single component, the composition of the antibacterial peptide and the plant extract has the advantages that the antibacterial and bactericidal effects are obviously improved, and the composition has an obvious antibacterial effect on propionibacterium and malassezia, and has an easy-to-comb function when being used in hair washing products. The composition has good effects of resisting bacteria, removing acnes and removing dandruff, is soft and easy to comb, and has storage stability.

The invention relates to the technical field of medicines, and particularly discloses a polypeptide Loni-P extracted from traditional Chinese medicine honeysuckle, and the amino acid sequence of the polypeptide Loni-P is SEQ ID No.1. The polypeptide can be prepared from honeysuckle through the steps of ultrasonication, enzymolysis, purification and the like. Experiments show that the inhibition rate of Loni-P to malassezia under the concentration of 2 mu g / mL reaches 86 + / -5.29%, which is similar to that of positive control ketoconazole (92 + / -9.17%). The invention further provides a medicated bath component containing Loni-P. The medicated bath component is composed of polypeptide, beta-cyclodextrin, chitosan, fumaric acid, sodium hydroxide and other auxiliary materials and is matched with 200 L of medicated bath water. Clinical experiments show that the medicated bath can remarkably relieve itching, skin lesion area and shape of a chronic eczema patient, and after 30 days of treatment, the score of an experimental group is reduced from 7.93 to 4.0 (a control group has no remarkable change). The polypeptide is natural in source and high in safety, and a new strategy is provided for eczema treatment.

The invention relates to the technical field of scalp care, and particularly provides an anti-dandruff composition containing sophora flavescens polypeptide as well as a preparation method and application of the anti-dandruff composition. The anti-dandruff composition comprises multiple active ingredients such as sophora flavescens polypeptide SoFla-I, salicylic acid, selenium disulfide, glutathione and nicotinamide, and is prepared through a specific process. The amino acid sequence of the sophora flavescens polypeptide SoFla-I is shown as SEQ ID No.1. The preparation method of the sophora flavescens polypeptide SoFla-I comprises the steps of freeze drying of sophora flavescens roots, ultrasonic extraction, ammonium sulfate precipitation, dialysis purification and the like. Experiments show that the composition can effectively inhibit malassezia and significantly improve dandruff and pruritus symptoms, the dandruff score and the pruritus score are significantly reduced after the composition is used for 14 days, and the satisfaction degree of subjects is high. The composition is high in safety, remarkable in anti-dandruff effect and suitable for shampoo products, scalp care agents and the like.

The invention discloses oil-controlling and dandruff-removing shampoo and a preparation method thereof. The shampoo comprises an anti-malassezia single-domain antibody with an amino acid sequence as shown in SEQ ID NO: 1 and an asparagus cochinchinensis root extract obtained through supercritical carbon dioxide extraction. The activity retention rate of the antibody is not lower than 80% after the antibody is stored in a shampoo matrix at 40 DEG C for 28 days. The total polyphenol content of the extract is not less than 5%. Chessboard microdilution determination shows that the antibody and the extract have a synergistic effect in the aspect of inhibiting malassezia, and the synergistic index is less than 0.9. Cell experiments show that the extract can down-regulate gene expression of 5 alpha-reductase in human sebaceous gland cells. A human body test result shows that after the shampoo is used for 4 weeks, the dandruff score of a subject is reduced by 82.5%, the scalp grease content is reduced by 64.1%, the malassezia quantity is reduced by 47.8%, and the effect is superior to that of a single component and a commercially available chemical dandruff removing product. The shampoo has excellent stability, synergistic effect and clinical application effect, and has a relatively high industrial application prospect.

The invention relates to the technical field of Chinese arborvitae twig and leaf extracts, and discloses a Chinese arborvitae twig and leaf extract-containing anti-dandruff and itching-relieving plant composition and a preparation method thereof, and the method comprises the following steps: preparation of a Chinese arborvitae twig and leaf ethanol extract, preparation of a water phase, preparation of an oil phase, emulsification, blending and post-treatment. A multi-target synergistic effect system is constructed by carefully selecting a Chinese arborvitae twig and leaf ethanol extract, curcumin, dipotassium glycyrrhizinate and oat beta-glucan and scientifically compounding; by utilizing the antibacterial and bacteriostatic properties of the cacumen biotae ethanol extract, the anti-inflammatory and anti-oxidation characteristics of curcumin, the soothing and repairing effects of dipotassium glycyrrhizinate and the barrier protection function of oat beta-glucan, malassezia reproduction can be inhibited, scalp inflammatory response can be relieved, and the damaged scalp barrier can be repaired, so that dandruff removal and lasting itching relieving are realized; meanwhile, all the components are natural, so that scalp irritation and drug resistance risks caused by chemical synthesis components are reduced, and the mildness and long-term use safety of the product are guaranteed.

This invention relates to a polymyxin Bacillus strain ( Paenibacillus polymyxa This invention relates to B01 and its applications, belonging to the field of microbial technology. The *Bacillus polymyxa* B01 strain disclosed in this invention has been deposited at the Guangdong Provincial Microbial Culture Collection Center, with accession number GDMCC NO.66594. This strain was isolated, purified, and screened from soil samples. Experiments show that this strain has excellent pH and high-temperature tolerance, and the antibacterial active substances produced by its fermentation have good antibacterial effects against *Escherichia coli*, *Pseudomonas aeruginosa*, *Pseudomonas putida*, *Staphylococcus aureus*, *Candida albicans*, *Aspergillus brasiliensis*, *Propionibacterium acnes*, and *Malassezia*. Furthermore, the antibacterial active substances exhibit excellent heat resistance, cold resistance, light stability, pH stability, and preservative effects. It is a mild and low-irritant raw material that can be used to prepare daily chemical products and pharmaceuticals, possessing broad application prospects and significant value for transformational research.

The invention discloses long-acting anti-dandruff shampoo based on microbial balance regulation and a preparation method of the long-acting anti-dandruff shampoo. The shampoo comprises a cleaning system composed of sodium lauroyl sarcosinate, cocamidopropyl betaine and decyl glucoside, a micro-ecological regulation system composed of piroctone olamine, inulin and a lactobacillus fermentation product, and skin barrier repairing lipids such as ceramide and squalane. According to the preparation method, all the components are effectively integrated through a step-by-step temperature control and homogeneous emulsification process. Through the synergistic effect of the components, malassezia is efficiently inhibited, effective microbial communities on scalp are selectively maintained, and a skin barrier is repaired, so that scalp micro-ecological balance is regulated and controlled, long-acting dandruff removal is realized, the scalp health level is improved, and the scalp care effect is improved. The problems that a traditional anti-dandruff product is likely to cause dysbacteriosis, the effect is not lasting, and relapse is caused after the traditional anti-dandruff product is stopped are solved.

The present invention relates to the technical field of microorganisms. Disclosed in the present invention are Lacticaseibacillus casei VB179 for inhibiting Malassezia, and a culture device and the use thereof. Specifically, disclosed in the present invention is Lacticaseibacillus casei VB179, wherein the Lacticaseibacillus casei VB179 is deposited in the China General Microbiological Culture Collection Center with a deposit number of CGMCC No. 28623 and a deposit date of 12 October 2023. The Lacticaseibacillus casei VB179 of the present invention can significantly inhibit the growth of Malassezia, and can further be used for treating skin diseases caused by the excessive reproduction of Malassezia, such as tinea versicolor, Malassezia folliculitis, seborrheic dermatitis and atopic dermatitis.

The application discloses Bacillus safensis HNXJ-B1 and application thereof, is preserved in China Microorganism Strain Preservation Management Committee general microorganism center, and its preservation number is: CGMCC NO: 32527, and the preservation date is November 7, 2024.The application is identified by 16S rDNA strain, and it is confirmed that HNXJ-B1 is Bacillus safensis.The inhibition effect on Ralstonia solanacearum, Diaporthe sclerotioides, Trichophyton mentagrophytes and Malassezia is obvious, can simultaneously inhibit plant disease pathogenic bacteria and animal pathogenic fungi, and has wide application prospect.

The present application relates to the technical field of scalp care, and specifically provides a dandruff-removing composition containing sophora flavescens polypeptide, a preparation method and application thereof.The dandruff-removing composition contains various active ingredients such as sophora flavescens polypeptide SoFla-I, salicylic acid, selenium disulfide, glutathione and nicotinamide, and is prepared through a specific process.The amino acid sequence of the sophora flavescens polypeptide SoFla-I is shown as SEQ ID No.1, and the preparation includes steps such as freeze-drying of sophora flavescens roots, ultrasonic extraction, ammonium sulfate precipitation and dialysis purification.Experiments show that the composition can effectively inhibit malassezia, and significantly improve dandruff and pruritus symptoms, and the dandruff score and pruritus score significantly decrease after 14 days of use, and the satisfaction of the subjects is high.The composition of the present application is high in safety and significant in dandruff-removing effect, and is suitable for shampoo products, scalp care agents and the like.

The invention discloses a preparation method of a monoclonal antibody for detecting trichophyton rubrum and application of the monoclonal antibody in fungal skin disease detection, and belongs to the field of biological medicine. The monoclonal antibody provided by the invention only has specific reaction with trichophyton rubrum, and has no cross reaction with common fungi such as trichophyton mentagrophytes, epidermophyton flocculent, candida albicans, malassezia and the like and normal flora of a human body, so that a false positive result is effectively avoided. Based on the double-antibody sandwich principle, the detection sensitivity can reach 200 pg / mL level, trace trichophyton rubrum in a clinical sample can be detected, and the positive rate is obviously higher than that of direct microscopic examination. The whole detection process can be completed within 15-20 min and is far faster than fungus culture for several weeks, a rapid diagnosis basis can be provided for doctors, and early treatment is facilitated. The kit provided by the invention has standardized operation steps, does not need complex instruments and special training, and is suitable for popularization and use in clinical laboratories of hospitals at all levels, outpatient clinics of dermatology and even basic medical institutions.

This invention discloses a composition with hair-care effects on damaged hair and its application, comprising *Ulva prolifera* extract, *Angelica sinensis* extract, *Lichen davidii* extract, and *Camellia chrysantha* extract, wherein the weight ratio of the *Ulva prolifera* extract, *Angelica sinensis* extract, *Lichen davidii* extract, and *Camellia chrysantha* extract is (1-3):(1-2):(3-7):(5-8). It has the effects of repairing hair cuticles, nourishing hair, improving hair shine, improving combability, scavenging free radicals, and inhibiting Malassezia.

The invention provides traditional Chinese medicine shampoo for treating oily alopecia and a preparation method of the traditional Chinese medicine shampoo. The traditional Chinese medicine shampoo is prepared from the following raw materials in parts by weight: 20 to 28 parts of spica prunellae, 20 to 25 parts of herba verbenae, 18 to 24 parts of herba equiseti hiemalis, 6 to 10 parts of herba swertiae mileensis, 9 to 13 parts of radix notoginseng, 8 to 15 parts of fructus arctii, 16 to 22 parts of Chinese usnea, 16 to 20 parts of radix ligustici sinensis, 9 to 13 parts of fructus tribuli, 9 to 13 parts of cacumen biotae, 10 to 15 parts of radix sophorae flavescentis, 9 to 14 parts of Chinese soapberry fruit and 1 to 3 parts of thickening agent. The traditional Chinese medicine shampoo is prepared from selfheal, verbena, horsetail, swertia mileensis, pseudo-ginseng, burdock, Chinese usnea, ligusticum, tribulus terrestris and other raw materials, the problem that malassezia and the like affect scalp micro-ecological imbalance can be effectively inhibited through the synergistic effect of active ingredients in all the raw materials, the scalp microenvironment is improved, sebum secretion is inhibited, hair growth is promoted, and the hair loss is reduced. The proliferation of hair follicle cells is promoted, so that a good effect of treating oily alopecia is achieved.

The invention discloses a plant composition for regulating scalp micro-ecology and application thereof, and relates to the technical field of cosmetics. The invention provides a plant composition which is prepared from magnolia bark and uncaria rhynchophylla, and the feeding ratio of the magnolia bark to the uncaria rhynchophylla is 7: 3-3: 7. The plant composition has the effect of synergistically promoting expression of scalp keratinocyte human beta-defensin, has an inhibiting effect on malassezia and does not have an inhibiting effect on staphylococcus epidermidis under the concentration of 0.5%-2%, has the effect of adjusting scalp flora balance, and can relieve the degree of scalp itching and reduce the number of scurf.

The invention discloses an anti-alopecia emulsion and a production process thereof, and relates to the technical field of daily chemical products, in order to improve the anti-alopecia effect of the shampoo, a bacteriostatic agent component is prepared and added, and the combination of a bacteriostatic agent and skin lipid is effectively improved in a manner of generating a silane long chain segment in the bacteriostatic agent component, so that the anti-alopecia effect of the shampoo is improved, and the anti-alopecia effect of the shampoo is improved. The active substances can enter the hair follicles conveniently, so that the activity of malassezia is inhibited, the active substances can be prevented from being taken away by flowing water during washing by combining with lipid, and the active substances can be retained on the skin surface for a long time to inhibit the reproduction of malassezia; the thiophene ring, the pyridine ring, the halamine group and other structures have excellent antibacterial activity and can inhibit fungal proliferation activity, so that the antibacterial effect on malassezia is effectively improved, and the problem of alopecia caused by malassezia reproduction is avoided.

An aspect of the present application relates to a peptide having antibacterial activity against Malassezia, an antibacterial composition including the same, and an antibacterial preparation including the same. The peptide composition having a microorganism group modulating ability of the scalp of the present application shows antibacterial activity against Malassezia, and is helpful to effectively improve the microbial balance of the scalp. Such antibacterial activity can provide a positive effect of enhancing the health of the scalp environment, preventing hair loss, and relieving scalp-related problems.

The invention relates to a plant anti-dandruff composition with oil control and bacteriostasis effects and application of the plant anti-dandruff composition, and belongs to the technical field of cosmetics. The composition comprises a soapberry fruit extract, a camellia seed extract, an artemisia annua extract, a valerian root extract and an emblic leafflower fruit extract. The radix valerianae extract is obtained by taking radix valerianae as a raw material, inoculating a mixed bacterial agent composed of bacillus licheniformis and lactobacillus bulgaricus, fermenting and then extracting. The radix valerianae extract, which is extracted after the radix valerianae is mixed and fermented according to a specific strain ratio, has a remarkably improved antibacterial effect on malassezia and propionibacterium acnes. Furthermore, a specific soapberry fruit extract, a camellia seed extract, an artemisia annua extract, an emblic leafflower fruit extract and the obtained valerian root extract are selected to be combined in a specific proportion range, all the components in the obtained composition play a synergistic effect, and the oil control, antibacterial and anti-dandruff effects of the composition can be remarkably improved.

This application provides the use of an antimicrobial peptide in the preparation of skin care products. The antimicrobial peptide of this application can effectively inhibit *Propionibacterium acnes* and *Malassezia*, thereby preventing skin problems such as acne, skin inflammation, and dandruff caused by the overgrowth of these two bacteria. Therefore, the antimicrobial peptide of this application has skin care uses in inhibiting acne, relieving skin inflammation such as folliculitis, pityriasis versicolor, and seborrheic dermatitis, and in inhibiting dandruff, and has significant practical value.

This invention discloses the application of an artemisia annua compound as the sole active ingredient in the preparation of topical formulations for the prevention or improvement of blackheads and acne. Based on 100% of the total mass of the topical formulation, the artemisia annua compound accounts for 0.1% to 0.22%, and is composed of the following raw materials in parts by mass: camphene 4 to 10 parts, artemisinin 1 part. This invention also discloses a topical formulation for the prevention or improvement of blackheads and acne, the sole active ingredient of which is an artemisia annua compound. Based on 100% of the total mass of the topical formulation, the artemisia annua compound accounts for 0.1% to 0.22%, and is composed of the following raw materials in parts by mass: camphene 4 to 10 parts, artemisinin 1 part. The artemisia annua compound of this invention can significantly reduce the formation of squalene peroxide in a UVA-induced squalene photo-oxidation model, and shows stronger inhibition against Malassezia and less impact on Staphylococcus epidermidis in a Malassezia vs. symbiotic bacteria comparison model.

This invention addresses the prevalent hair loss problem in modern people by providing a pharmaceutical composition comprising Sanguisorba officinalis extract and Sophora flavescens extract. Topical application improves hair growth in a hair loss model mouse, resulting in thicker, shinier hair. Both hair weight and length show significant improvement compared to the model group, the Sanguisorba officinalis-only group, and the Sophora flavescens-only group, indicating synergistic effects. This may be related to its inhibition of Malassezia scalp, thereby improving the scalp environment. In particular, the co-decoction extract of Sanguisorba officinalis and Sophora flavescens shows superior efficacy compared to physically mixed Sanguisorba officinalis and Sophora flavescens extracts in the same proportion. Optimal efficacy was achieved at a Sanguisorba officinalis:Sophora flavescens weight ratio of 2:8, demonstrating the therapeutic advantage of the co-decoction extract. In volunteer use cases, the co-decoction extract showed similar excellent effects in improving scalp sebum secretion and narrowing the hair loss area, as well as a significant clearing effect on folliculitis.

The present application provides a kind of emulsion for improving pet skin problems and its preparation method, it is related to the technical field of traditional Chinese veterinary medicine pharmacy.The emulsion is prepared by emulsification homogenization from traditional Chinese medicine extract, traditional Chinese medicine essential oil, plant fatty acid, emulsifier and water, wherein the traditional Chinese medicine extract is Astragalus and ginseng extract, and the traditional Chinese medicine essential oil is a compound of clove basil oil, angelica oil, rose oil and kempas seed oil.The present application solves the problems of large irritability, easy drug resistance of chemical drugs and poor absorption of traditional Chinese medicine by synergistic compounding of active ingredients.The product has the functions of antibacterial, barrier repair, sebum regulation and moisturizing, and the inhibition zone diameters of staphylococcus aureus and malassezia are 12.5±0.5 mm and 10.2±0.3 mm respectively, and it can significantly improve skin moisturizing function, and is mild and non-irritating, suitable for the treatment and care of various skin problems of pets.