A plant composition for inhibiting malassezia and its preparation method and application

Abstract

This invention provides a plant composition, its preparation method, and its application, relating to the field of plant essential oil technology. The plant essential oils extracted from Alpinia oxyphylla, Agrimonia pilosa, coriander seeds, and Hibiscus syriacus bark have excellent inhibitory effects against Malassezia; they are also non-irritating to human skin and can be used in daily chemical hair care product formulations to effectively inhibit dandruff.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of plant composition, in particular to a plant composition for inhibiting malassezia and a preparation method and application thereof. BACKGROUND

[0002] At present, using effective components in natural plants for hair care and scalp care gradually becomes a research hotspot. For example, a patent document with the application publication number CN116115514A discloses a plant protein composition for hair care and a preparation method and application thereof. The plant protein composition uses plant essential oils extracted from fir, cedar and tea tree, and cooperates with hydrolyzed keratin to promote the penetration of hydrolyzed keratin into hair. For another example, a patent document with the application publication number CN115154370A discloses a hair growth, hair loss prevention and hair care essential oil composition and a preparation method thereof. The essential oil composition contains 10% to 50% rosemary essential oil, 10% to 50% lavender essential oil and 0.5% to 40% schisandra essential oil. The essential oil composition has the effects of hair growth, hair loss prevention, scalp care, antibiosis, anti-inflammation and the like in addition to the hair care effect.

[0003] Dandruff is a common skin disease characterized by flaky scales on the scalp and hair, and the incidence rate thereof in the population is about 50%. Dandruff can cause local itching, scalp irritation, scalp tightness and dryness and the like, and if not treated by proper methods, dandruff can occur periodically, recurrently or continuously, thereby causing troubles in daily work, life and social activities of people. Dandruff is medically known as pityriasis capitis, which is a skin disease caused by malassezia. At present, the main drugs for treating dandruff are chemical drugs such as selenium disulfide and zinc pyrithione, which often cause side effects such as skin redness, hair follicle damage and the like. Therefore, it is urgent to provide a technical solution for treating dandruff by using effective components in natural plants. SUMMARY

[0004] In order to achieve the above-mentioned application purposes, the present application provides the following technical solutions.

[0005] The present application provides a plant composition, which contains one or more of the following raw materials: Szechwan fagara, agrimony, coriander seed and mume bark.

[0006] The raw materials further include extracts of Szechwan fagara, agrimony, coriander seed and mume bark.

[0007] Preferably, the raw materials contain 30 to 60 parts of Szechwan fagara, 20 to 40 parts of agrimony, 10 to 30 parts of coriander seed and 10 to 30 parts of mume bark by weight.

[0008] Preferably, the Shizhi is derived from the dried mature fruits of Alpinia oxyphylla Miq., the Xianhecao is the dried above-ground parts of Herba Agrimoniae P., the Coriander seed is the mature seed of Coriandrum sativum L., and the Kuijinboki is the dried bark of Hibiscus syriacus L.

[0009] The Shizhi is derived from the dried mature fruits of Alpinia oxyphylla Miq., mainly distributed in some areas of southern China, and is praised as one of the "four large southern medicines" in China. Shizhi is rich in essential oil, and the round yuzhengnian is one of the main components of essential oil, and the content is high. Shizhi essential oil has been reported to have strong insect repellent effect, but no other activity has been reported; the team's previous experiments found that the essential oil of false Shizhi has excellent in vitro antibacterial activity.

[0010] The Xianhecao is the dried above-ground parts of Herba Agrimoniae P., bitter and astringent in taste, and neutral in nature, and belongs to the heart and liver channels, and is traditionally used to treat hemoptysis, malaria, carbuncle and sore, etc.; modern pharmacological studies have shown that Xianhecao has antitumor, analgesic and anti-inflammatory, insecticidal, hemostatic, antioxidant and other pharmacological effects. And has broad-spectrum anti-influenza virus activity. The application of Xianhecao in cosmetics has been reported, but there is no report on the inhibition of Malassezia in daily chemical products.

[0011] The Coriander seed is the mature seed of Coriandrum sativum L., which is warm in nature and pungent in taste, and enters the lung and spleen channels, and can induce sweating, remove rash, and promote digestion and lower qi, and has therapeutic effects on indigestion, cold and flu, and headache and fever, and it has also been reported that Coriander has a detoxification effect. Coriander seeds are rich in essential oil components, mainly linalool, and other components such as myrcene, alpha-pinene, limonene, etc. There have been reports that Coriander seed essential oil can be used to treat rheumatism, but there is no report on the inhibition of Malassezia in daily chemical products.

[0012] The Kuijinboki is the dried bark of Hibiscus syriacus L., and the Kuijin has rich food and medicinal value, and its flowers, roots and leaves can be used as medicine. It is believed in traditional Chinese medicine that Kuijin has the functions of clearing heat, removing dampness, and cooling blood, and can help prevent and treat hypertension and cardiovascular diseases, and can also regulate human immune function, and has anti-inflammatory, anticancer, antidiabetic, antioxidant, antihypertensive and other effects.

[0013] The application further provides a preparation method of the plant composition, wherein the mixture of Radix Alpiniae Oxophyllae, Herba Agrimoniae, Coriandrum sativum L. seeds and Mume Cortex is soaked, then is subjected to ultrasonic treatment, and then the volatile oil part is distilled to obtain the plant composition.

[0014] Or the Radix Alpiniae Oxophyllae, Herba Agrimoniae, Coriandrum sativum L. seeds and Mume Cortex are respectively soaked, then are subjected to ultrasonic treatment, and then the volatile oil parts are mixed to obtain the composition.

[0015] Preferably, the soaking is performed by adding water to the mixture and soaking for 12-48 h; the mass / volume ratio of the mixture to water is 1:10-30 g / mL.

[0016] Preferably, the ultrasonic treatment is performed at a power of 250-350 W, at a temperature of 40-70 DEG C, and for 30-60 min.

[0017] Preferably, the distillation is performed by using a water vapor distillation device, keeping a micro-boiling state, extracting for 2-4 h, and collecting the volatile oil part.

[0018] The application further provides a use of the plant composition in the preparation of an antibacterial product.

[0019] The application further provides a use of the plant composition in the preparation of a daily chemical product.

[0020] Preferably, the daily chemical product comprises shampoo, hair conditioner, hair care essential oil, scalp essence or hair mask.

[0021] The application further provides a shampoo, which comprises the above-mentioned composition at 0.01%-10% by mass percentage. Preferably, the composition is 1%.

[0022] The application has the following advantages:

[0023] (1) The application utilizes the traditional Chinese medicine principle of "monarch, minister, assistant and guide" to formulate the composition, wherein the Radix Alpiniae Oxophyllae is the monarch drug, has excellent antibacterial effect, the Herba Agrimoniae is the minister drug, has good anti-inflammatory and antibacterial effect, the Coriandrum sativum L. seeds are the assistant drug, have the effect of dispelling wind and eliminating stagnation, and the Mume Cortex is the guide drug, harmonizes the drugs, has the effects of clearing heat and resisting oxidation, can improve the skin balance of the damaged part, and promotes skin repair. After the four kinds of plant raw materials with different effects are formulated and compounded, the synergistic effect is achieved; the volatile oil obtained by common distillation has the special effects of antibacterial, heat-clearing, anti-inflammatory and antioxidant; the extraction and separation method is simple, the process is green and environmentally friendly, and the obtained product has high effective substance content. The physiological activity detection of the plant compound essential oil shows that the plant compound essential oil has remarkable effect on inhibiting Malassezia.

[0024] (2) The preparation method is simple and easy to operate, and the raw material volatile oil part is completely extracted. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 : Plant compound essential oil, single essential oil inhibition rate of malassezia column chart. DETAILED DESCRIPTION

[0026] The application provides a plant composition, which comprises one or more of Alpinia oxyphylla Miq., Agrimonia pilae, Coriandrum sativum L. and Hibiscus syriacus L.

[0027] The raw materials further comprise extracts of Alpinia oxyphylla Miq., Agrimonia pilae, Coriandrum sativum L. and Hibiscus syriacus L.

[0028] Preferably, the plant composition comprises essential oil extracted from a mixture of Alpinia oxyphylla Miq., Agrimonia pilae, Coriandrum sativum L. and Hibiscus syriacus L.

[0029] Preferably, the raw materials in the combination composition are Alpinia oxyphylla Miq. 30-60 parts, Agrimonia pilae 20-40 parts, Coriandrum sativum L. 10-30 parts and Hibiscus syriacus L. 10-30 parts by mass.

[0030] Optionally, the mass ratio of Alpinia oxyphylla Miq., Agrimonia pilae, Coriandrum sativum L. and Hibiscus syriacus L. is 40:30:15:15.

[0031] Optionally, the mass ratio of Alpinia oxyphylla Miq., Agrimonia pilae, Coriandrum sativum L. and Hibiscus syriacus L. is 60:20:10:10.

[0032] Optionally, the mass ratio of Alpinia oxyphylla Miq., Agrimonia pilae, Coriandrum sativum L. and Hibiscus syriacus L. is 30:30:20:20.

[0033] More preferably, Alpinia oxyphylla Miq. is derived from the dry mature fruits of Alpinia oxyphylla Miq., Agrimonia pilae is the dry above-ground part of Herba Agrimoniae P., Coriandrum sativum L. is the mature seeds of Coriandrum sativum L. and Hibiscus syriacus L. is the dry bark of Hibiscus syriacus L.

[0034] The application further provides a preparation method of the plant composition, wherein the mixture of Alpinia oxyphylla Miq., Agrimonia pilae, Coriandrum sativum L. and Hibiscus syriacus L. is soaked, then ultrasonically treated, and then distilled to obtain the volatile oil part to obtain the composition.

[0035] Or the above Jizhi, magic crane grass, coriander seeds and hibiscus bark are respectively soaked, then ultrasonic treated, and then the volatile oil part is distilled to obtain the composition.

[0036] Preferably, the soaking method is to add water to the mixture and soak for 12-48h; the mass / volume ratio of the mixture to water is 1:10-30g / mL.

[0037] Preferably, the ultrasonic treatment power is 250-350W, the temperature is set to 40-70℃, and the treatment time is 30-60min.

[0038] Preferably, the distillation process is to use a water vapor distillation device, keep the state of micro-boiling, extract for 2-4h, and collect the volatile oil part.

[0039] The application also provides a use of the plant composition in the preparation of an antibacterial product.

[0040] The application also provides a use of the plant composition in the preparation of a daily chemical product.

[0041] Preferably, the daily chemical product includes shampoo, hair conditioner, hair care essential oil, scalp essence or hair mask.

[0042] The application also provides a shampoo, which contains the plant composition 0.01%-10% by mass percentage. Preferably, the plant composition is 1%.

[0043] In the application, all raw material components are commercially available products known to those skilled in the art, unless otherwise specified.

[0044] The technical solutions in the application will be described clearly and completely in combination with the embodiments in the application. Obviously, the described embodiments are only some of the embodiments of the application, but not all the embodiments. Based on the embodiments in the application, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the application.

[0045] Embodiment 1

[0046] Mixing Fructus Schisandrae, Agrimoniae Herba, Coriandri Semen, and Mume Cortex, wherein Fructus Schisandrae, Agrimoniae Herba, Coriandri Semen, and Mume Cortex account for 40 g, 30 g, 15 g, and 15 g respectively in 100 g of the mixture; adding 2 kg of deionized water; then performing ultrasonic oscillation, with an ultrasonic power of 400 W, a temperature of 50 ℃, and an ultrasonic oscillation time of 45 min; after the ultrasonic oscillation, transferring all the materials to a water vapor distillation device, continuously heating to boiling, and keeping a state of gentle boiling for 3 h of continuous extraction; after the extraction is completed, distilling and collecting the distilled volatile oil part, which is a plant composition, referred to as a plant compound essential oil.

[0047]

[0048] The compound essential oil is light yellow and clear, with an extraction rate of 0.672%; its physicochemical properties are determined, with a refractive index (20 ℃) of 1.4571 and a relative density (20 ℃) of 0.886.

[0049] Example 2

[0050] Mixing Fructus Schisandrae, Agrimoniae Herba, Coriandri Semen, and Mume Cortex, wherein Fructus Schisandrae, Agrimoniae Herba, Coriandri Semen, and Mume Cortex account for 60 g, 20 g, 10 g, and 10 g respectively in 100 g of the mixture; adding 1 kg of deionized water; then performing ultrasonic oscillation, with an ultrasonic power of 300 W, a temperature of 40 ℃, and an ultrasonic oscillation time of 60 min; after the ultrasonic oscillation, transferring all the materials to a water vapor distillation device, continuously heating to boiling, and keeping a state of gentle boiling for 4 h of continuous extraction; after the extraction is completed, distilling and collecting the distilled volatile oil part, which is a plant composition, referred to as a plant compound essential oil.

[0051] The compound essential oil is light yellow and clear, with an extraction rate of 0.653%; its physicochemical properties are determined, with a refractive index (20 ℃) of 1.4570 and a relative density (20 ℃) of 0.886.

[0052] Example 3

[0053] Mixing Fructus Schisandrae, Agrimoniae Herba, Coriandri Semen, and Mume Cortex, wherein Fructus Schisandrae, Agrimoniae Herba, Coriandri Semen, and Mume Cortex account for 30 g, 30 g, 20 g, and 20 g respectively in 100 g of the mixture; adding 3 kg of deionized water; then performing ultrasonic oscillation, with an ultrasonic power of 600 W, a temperature of 60 ℃, and an ultrasonic oscillation time of 30 min; after the ultrasonic oscillation, transferring all the materials to a water vapor distillation device, continuously heating to boiling, and keeping a state of gentle boiling for 2 h of continuous extraction; after the extraction is completed, distilling and collecting the distilled volatile oil part, which is a plant composition, referred to as a plant compound essential oil.

[0054] The compound essential oil is light yellow and clear, and the yield of the essential oil is 0.649%. The physical and chemical properties of the essential oil are determined, and the refractive index (20℃) is 1.4571, and the relative density (20℃) is 0.887.

[0055] Comparative example

[0056] 100g of Schisandra chinensis, 100g of Agrimonia pilosa, 100g of Cuminum cyminum seeds and 100g of Hibiscus syriacus bark were respectively numbered as Comparative Examples 1-4. The volatile oils of Schisandra chinensis, Agrimonia pilosa, Cuminum cyminum seeds and Hibiscus syriacus bark corresponding to Comparative Examples 1-4 were respectively prepared according to the preparation method described in Example 1.

[0057] Experimental example 1

[0058] The 5% concentration of the plant compound essential oil and the plant single essential oil inhibits Malassezia furfur experiment:

[0059] 1. Experimental materials:

[0060] 1.1 Experimental strains:

[0061] Malassezia furfur belongs to anaerobic or facultative anaerobic bacteria. The strain was purchased from China Microbial Culture Collection Center, and the preservation number is ATCC1452.

[0062] 1.2 Experimental apparatus:

[0063] spoon, centrifuge tube (50mL, 10mL), conical flask (250mL), gun head (200uL, 1000uL), EP tube, sterile gloves, sealing film, culture dish, coating rod, scalpel, long handle forceps, incubator, spectrophotometer, microscope, clean bench, centrifuge, sterilization pot. The above experimental apparatus is the routine apparatus in the laboratory.

[0064] 1.3 Malassezia furfur culture medium and culture conditions:

[0065] Culture medium: beef extract 2g / L, proteose peptone 1.5g / L, L-cystine 0.5g / L, agar 15g / L (liquid medium without), sterilization 121℃, 15min. After sterilization, the culture bottle and test tube are taken out in time and the cap is tightened to reduce air entering the culture medium.

[0066] Malassezia furfur culture: the bacteria grow on the plate medium and present white and yellowish colonies with smooth and wet morphology. A ring of the bacteria on the slant or plate is picked up with an inoculation needle and inoculated into the liquid culture medium. The test tube is closed, shaken at 37℃ for 24-36h, and Malassezia furfur liquid is obtained.

[0067] Filter paper preparation: a puncher is used to punch the filter paper on three layers of folded filter paper, and the filter paper is placed in a box for 121℃ sterilization for standby use.

[0068] Preparation of the test solution: the plant essential oil sample prepared in each example is dissolved in DMSO and then diluted with deionized water to prepare a test solution with a concentration of 5%.

[0069] 1.4 Determination method:

[0070] Malassezia inhibitory experiment (filter paper bacteriostatic circle method): a certain amount of Malassezia liquid is injected into a flat medium cooled to about 50°C, mixed uniformly, poured into a flat plate (about 20 mL / plate), and horizontally placed until solidification. Then, sterile filter paper pieces are uniformly placed on the flat plate medium, 4-5 pieces per culture dish, and 20 uL of the plant essential oil sample to be tested is dropped onto the filter paper pieces using a pipette gun. Then, the culture is placed in an anaerobic tank for culture, and the change in the bacteriostatic circle is observed after 24-36 h, and the diameter of the bacteriostatic circle is recorded.

[0071] 2 Experimental results:

[0072] Table 1 Diameter of the bacteriostatic circle of the plant compound essential oil and the plant single essential oil on Malassezia

[0073] No. Inhibition zone diameter (mm) No. 1: Compound essential oil (5%) 17.3 No. 2: Compound essential oil (5%) 16.8 No. 3: Compound essential oil (5%) 16.7 No. 4: Yizhi essential oil (5%) 14.3 No. 5: Xianhecao essential oil (5%) 11.5 No. 6: Coriander seed essential oil (5%) 13.6 No. 7: Mumei essential oil (5%) 12.1

[0074] The experimental results are shown in Table 1 and Figure 1 Table 2: 1-3 compound essential oils correspond to the compound essential oils prepared according to Examples 1-3, and 4-7 single essential oils correspond to the single essential oils prepared from single plant components in the comparative examples.

[0075] As can be seen from Table 1 and Figure 1 Table 2, the plant compound essential oil has a significant inhibitory effect on Malassezia at a concentration of 5%; the four plant single essential oils also have a good inhibitory effect on Malassezia at a concentration of 5%; thus, the plant compound essential oil prepared in the present application has a significant inhibitory effect on Malassezia, and is much better than the corresponding single essential oil, which indicates that the plant components play a synergistic effect after being combined.

[0076] Experimental Example 2

[0077] Measurement of the MIC value of the plant compound essential oil on Malassezia:

[0078] 1 Materials and methods:

[0079] 1.1 Experimental materials:

[0080] The same as in Experimental Example 1.

[0081] 1.2 Experimental method:

[0082] The tube dilution method.

[0083] 1.2.1 Preparation of the Malassezia liquid medium:

[0084] The same as Experimental Example 1.

[0085] 1.2.2 Preparation of the test solution:

[0086] The same as Experimental Example 1.

[0087] 1.2.3 Preparation of the bacterial solution:

[0088] The Malassezia was subcultured for 2 times, and after the second subculture at 35°C for 2 days, the purified colonies were washed with sterile normal saline to prepare a bacterial suspension, and the concentration was adjusted to the McFarland No. 4 turbidity tube. The concentration of the bacterial solution was (1-2) x 10 7 cfu / mL, and the bacterial solution was diluted 10 times with the Malassezia liquid medium to make the concentration of the bacterial solution (1-2) x 10 6 cfu / mL.

[0089] 1.2.4 Sample addition of the test solution and result interpretation:

[0090] ① Take 10 test tubes, and add 2 mL of the Malassezia liquid medium to the first to the tenth test tubes.

[0091] ② Add 2 mL of the test solution to the first test tube and mix well.

[0092] ③ Take 2 mL of the mixed solution from the first test tube and add it to the second test tube, mix well; then take 2 mL of the mixed solution from the second test tube and add it to the next test tube, and so on to dilute the test solution by the ratio, and discard 2 mL at the ninth test tube. At this time, the concentrations of the test solutions in the test tubes are 1 / 2, 1 / 4, 1 / 8, 1 / 16, 1 / 32, 1 / 64, 1 / 128, 1 / 256, and 1 / 512 of the original solution, respectively.

[0093] ④ Add 100 uL of the test bacterial suspension to the first to the eighth and the tenth test tubes.

[0094] ⑤ Take another 10 test tubes (numbered 1-10), and prepare them in the same way as above, and discard 2 mL at the ninth test tube, without adding the bacterial suspension, as the negative control. The tenth test tube is the positive control.

[0095] ⑥ Incubate the 20 test tubes in a constant temperature incubator at 35°C for 48 h, and observe the turbidity by naked eye. The negative control tubes without bacterial growth are the MIC values of the test product to the test bacteria. Take the average of 3 measurements.

[0096] The determination results show that the MIC value of the test sample, i.e., the compound plant essential oil prepared in Example 1, to Malassezia is 0.625%, i.e., 6.25 mg / mL.

[0097] Example 4

[0098] Preparation of an antibacterial and dandruff-removing shampoo with plant compound essential oil as the main functional ingredient:

[0099] Preparation of an antibacterial and dandruff-removing shampoo containing the plant compound essential oil, the formula is shown in Table 2.

[0100] Table 2 Formula of antibacterial and dandruff-removing shampoo

[0101]

[0102]

[0103] Preparation process:

[0104] 1. After the cationic polymer (A phase) is uniformly dispersed in cold water, it is heated to 80℃, and after complete swelling, the B phase components are added in sequence, and stirred uniformly;

[0105] 2. After cooling to 60℃, the pre-mixed C phase and D phase are added, and stirred uniformly;

[0106] 3. Add E phase, adjust the pH of the system;

[0107] 4. After cooling to 45℃, the F phase components are added in sequence, and stirred uniformly to obtain the product.

[0108] The antibacterial and dandruff-removing shampoo is an embodiment of the plant composition prepared by the present application in hair care products, and can also be prepared into hair care products such as shampoo, hair conditioner, hair care oil, hair mask, etc. with antibacterial and dandruff-removing effects according to the commonly used formula in the hair care industry.

[0109] Experimental Example 3

[0110] The shampoo prepared in Example 4 is divided into 40 portions and placed in 200mL test packaging bottles, and is given to the test population (40 people, including 26 men and 14 women) for use. After continuous use for 4 weeks, the testers score the questionnaire test items according to the use experience. The scoring criteria are set as three indicators of “inhibition of head itching”, “dandruff-removing effect”, and “moisturizing effect”, using a 10-point system, with 1 point being the worst and 10 points being the best, and the higher the score, the better.

[0111] Evaluation results: the average score of “inhibition of head itching” is 8.2 points; the average score of “dandruff-removing effect” is 8.8 points; the average score of “moisturizing effect” is 8.0 points; and no one reports adverse reactions such as scalp allergy and redness. It shows that the antibacterial and dandruff-removing shampoo prepared by the present application has good actual effect.

[0112] The preparation method is simple and easy to operate, and the raw material volatile oil part is completely extracted. The plant composition of the present application is subjected to in vitro inhibition of Malassezia experiment, and the results show that the plant composition has excellent inhibitory effect; when applied to the formula of daily chemical products for hair, it can effectively play the role of inhibiting bacteria and removing dandruff. And the plant component raw material of the embodiment is simple to obtain and low in cost.

[0113] The above only describes the preferred embodiments of the present application, and it should be noted that those skilled in the art can make several improvements and refinements without departing from the principles of the present application, and these improvements and refinements should also be considered as the protection scope of the present application.

Claims

1. A plant composition for inhibiting Malassezia, characterized by comprising, The plant composition is prepared from 30-60 parts of Alpinia oxyphylla, 20-40 parts of Agrimonia pilosa, 10-30 parts of Coriandrum sativum, and 10-30 parts of Hibiscus syriacus bark. The plant composition is prepared by soaking the mixture of Alpinia oxyphylla, Agrimonia pilosa, Coriandrum sativum, and Hibiscus syriacus bark, then performing ultrasonic treatment, and distilling the volatile oil part. Or the plant composition is prepared by separately soaking Alpinia oxyphylla, Agrimonia pilosa, Coriandrum sativum, and Hibiscus syriacus bark, then performing ultrasonic treatment, and mixing the distilled volatile oil parts.

2. The method of preparing a plant composition according to claim 1, wherein The plant composition is prepared by soaking the mixture of Alpinia oxyphylla, Agrimonia pilosa, Coriandrum sativum, and Hibiscus syriacus bark, then performing ultrasonic treatment, and distilling the volatile oil part. Or the plant composition is prepared by separately soaking Alpinia oxyphylla, Agrimonia pilosa, Coriandrum sativum, and Hibiscus syriacus bark, then performing ultrasonic treatment, and mixing the distilled volatile oil parts.

3. The method of claim 2, wherein, The soaking is performed by adding water to the mixture and soaking for 12-48 hours; the mass / volume ratio of the mixture to water is 1:10-30 g / mL.

4. The method of claim 2, wherein, The ultrasonic treatment is performed at a power of 250-350 W, a temperature of 40-70°C, and for 30-60 minutes.

5. The method of claim 2, wherein, The distillation is performed using a water vapor distillation device, under a micro-boiling state, for 2-4 hours, and the volatile oil part is collected.

6. Use of the plant composition of claim 1 or the plant composition prepared by the method of any one of claims 2-5 in the preparation of a product for inhibiting Malassezia.

7. Use of the plant composition of claim 1 or the plant composition prepared by the method of any one of claims 2-5 in the preparation of a daily-use product.

8. A shampoo, characterized by, The shampoo comprises 0.01%-10% of the plant composition by mass percentage, wherein the plant composition is the plant composition of claim 1 or the plant composition prepared by the method of any one of claims 2-5.

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