Fermented anti-dandruff agent composition, preparation method and application

Abstract

The invention discloses a fermented anti-dandruff agent composition, a preparation method and application, and belongs to the field of daily chemicals. The fermented anti-dandruff agent is prepared from polylysine, a soothing agent, alcohol and water. The polylysine is obtained by a microbiological method; the polymerization degree range of the polylysine is specifically divided into 8 to 20, 8 to 30, 30 to 40, 8 to 20 and 8 to 30, and 8 to 20 and 30 to 40. The polylysine disclosed by the invention has excellent antibacterial performance, the antibacterial effects of structures with different polymerization degrees on bacteria and malassezia have selectivity, the biocompatibility is good, and the polylysine and degradation products thereof are safe and non-toxic and are safe to human bodies and environments. The polylysine disclosed by the invention is heat-stable, so that the polylysine can still be subjected to heat treatment after being added into the daily chemical product.

Description

Technical Field

[0001] The present invention relates to the field of daily chemicals, and in particular to a fermented anti-dandruff agent composition, a preparation method and an application thereof. Background Art

[0002] With the sudden increase in social pressure today, scalp problems are becoming increasingly prominent. Factors affecting scalp health include: 1) Genetic factors. Family inheritance may affect excessive oil secretion of the scalp during adolescence or adulthood, increasing the risk of dandruff; 2) Hormonal changes. Hormonal fluctuations during adolescence, menstruation, etc. will stimulate the secretion of sebaceous glands, aggravating oily scalp and dandruff problems; 3) Dietary habits. A high-sugar, high-fat diet can easily lead to endocrine disorders and aggravate scalp oil secretion; a balanced diet (rich in fruits and vegetables, high-quality protein) helps maintain scalp health; 4) Lifestyle. Lack of sleep and high mental stress may cause endocrine disorders, indirectly leading to scalp problems; 5) Environmental factors. Air pollution and climate change (dry or humid) can irritate the scalp, inducing itching, inflammation and dandruff.

[0003] Dandruff is a product of the metabolism of epidermal cells on the human head. This physiological process is called keratinization. Cells form in the basal layer of the epidermis, proliferate, and divide. Gradually, as they move upward, the cells gradually form keratin, creating a nucleusless, lifeless stratum corneum. These dry, dead cells shed spontaneously in the form of scales or flakes, commonly known as dandruff. There are several theories about the cause of dandruff, the most important of which is the mechanism of action of Malassezia fungi. Malassezia is a lipophilic, single-celled yeast. Numerous studies have shown that Malassezia is closely associated with the occurrence and severity of dandruff. They feed on the oils in the scalp and thrive on sebum-rich skin. When Malassezia multiplies on the scalp, it causes excessive proliferation of the stratum corneum, prompting abnormal shedding of keratinized cells in the form of white or gray scales. These shed scales are dandruff.

[0004] The mechanism of action of Malassezia in the etiology of dandruff: Malassezia on the scalp releases lipase to break down triglycerides in sebum, triggering hydrolysis of triglycerides to produce unsaturated fatty acids. These damage the scalp's barrier, the stratum corneum, causing unsaturated fatty acids to penetrate the epidermis and stimulate an inflammatory response. Their metabolites (such as free fatty acids) stimulate the scalp's immune response, releasing inflammatory factors (such as IL-1α and IL-8), further damaging the scalp's microbiome.

[0005] The research on dandruff has always focused on the fungus Malassezia, while neglecting the role of bacteria. Compared with normal scalps, when dandruff is present, the number of Propionibacterium on the scalp decreases while the number of Staphylococcus increases, and the diversity of the scalp microbiota in dandruff decreases. Research has found that the number of Staphylococcus epidermidis on the surface of the scalps of dandruff patients is significantly higher than that of healthy people, which means that Staphylococcus epidermidis may be involved in the pathogenesis of dandruff. Propionibacterium can secrete bacteriocins to inhibit the growth of Staphylococcus, while Staphylococcus mediates the fermentation of glycerol in the skin to inhibit the overgrowth of Propionibacterium.

[0006] At present, for the problems of dandruff and dermatitis caused by Malassezia, the mainstream antidandruff agents used in the market include piroctone olamine (OCT), zinc pyrithione (ZPT), selenium disulfide, etc. Among them, OCT has good antidandruff effect but high production cost, is prone to discoloration when encountering metal ions and light, and its irritation can cause scalp itching in some users; ZPT cannot be applied to transparent shampoos and products containing chelating agents such as EDTA, and is prohibited in the European Union; selenium disulfide has strong irritation, color and odor, and is insoluble in water.

[0007] Patent CN112675056A provides a shampoo with antidandruff and soothing effects, but it simply dissolves piroctone olamine in a surfactant and then physically mixes it with plant extracts. The steps in the production and preparation of the product are troublesome, and various aqueous and oily raw materials need to be mixed in a specific order, and it cannot provide a raw material with wide applicability and good compatibility. In addition, various chemical raw materials have greater irritation to the scalp, further affecting scalp health; and its use is also restricted by the limited standards in the "List of Cosmetic Raw Materials in Use (2021 Edition)".

[0008] Therefore, based on the problems existing in the current daily chemical market, there is an urgent need to develop a raw material that is safe and natural in source, has good antibacterial effects on Malassezia and related bacteria, is soothing and has little irritation at the same time, and this raw material needs to have good compatibility in daily chemical products to meet the market demand. Summary of the Invention

[0009] In view of the above problems, the purpose of the present invention is to provide a fermented antidandruff agent composition, a preparation method and an application. The composition is natural in source, has good water solubility and biological safety, has high antibacterial performance against Malassezia and bacteria, can effectively reduce the irritation to the scalp, has good compatibility in daily chemical products, and has a wide range of application fields.

[0010] To achieve the above purpose, the technical solution of the present invention is as follows:

[0011] A fermented antidandruff agent composition, wherein the fermented antidandruff agent is composed of polylysine, a soothing agent, alcohols and water.

[0012] The polylysine is obtained by the microbial method;

[0013] Furthermore, the polymerization degree range of the polylysine is specifically divided into 8 - 20, 8 - 30, 30 - 40, 8 - 20 and 8 - 30, 8 - 20 and 30 - 40.

[0014] The soothing agent is one or a combination of two or more of bisabolol, trans - 4 - tert - butylcyclohexanol, hydroxyphenyl propionamide benzoic acid, Poria cocos sclerotium extract, Centella asiatica extract, Piper wallichii root extract, Sophora flavescens extract, and Portulaca oleracea extract.

[0015] The alcohols are one or a combination of two or more of isopropyl alcohol, propylene glycol, butylene glycol, 1,2 - pentanediol, hexylene glycol, polyethylene glycol, and polypropylene glycol;

[0016] Furthermore, the alcohol is butylene glycol.

[0017] The mass ratio of the polylysine, soothing agent, alcohols, and water is (5 - 30):(0.1 - 1):(10 - 40):(45 - 70).

[0018] A preparation method of a fermentation anti - dandruff agent composition, the preparation method comprising the following steps:

[0019] (1) Mix and stir the soothing agent and alcohols according to the ratio until completely dissolved;

[0020] (2) Add polylysine to water and stir until completely dissolved;

[0021] (3) Mix the solutions of steps (1) and (2), and continue to stir until uniform to obtain the fermentation anti - dandruff agent composition;

[0022] Furthermore, the temperature in step (1) is maintained at 40 - 50 °C, and the pH of the solution system in step (3) needs to be maintained at 4 - 9.

[0023] The fermentation anti - dandruff agent composition prepared by the described preparation method has an obvious inhibitory effect on Malassezia.

[0024] An application of a fermentation anti - dandruff agent composition, mainly applied to anti - dandruff products.

[0025] The beneficial effects of the present invention:

[0026] (1) The polylysine of the present invention has excellent antibacterial properties. The structures with different polymerization degrees have selectivity for the antibacterial effects on bacteria and Malassezia, and have good biocompatibility. The polylysine itself and its degradation products are safe and non - toxic, and are safe for the human body and the environment.

[0027] (2) The polylysine of the present invention is heat-stable, so it can still be heat-treated after being added to daily chemical products.

[0028] (3) The soothing agent of the present invention can form a stable composition with polylysine in an aqueous alcohol solution in an appropriate proportion, effectively reducing the irritation to the scalp.

[0029] (4) The fermentation anti-dandruff agent composition of the present invention complies with the limited standard in the "List of Cosmetic Raw Materials in Use (2021 Edition)", is applicable to various types of daily chemical products, and has excellent compatibility.

[0030] (5) The preparation process of the fermentation anti-dandruff agent composition of the present invention is simple, suitable for industrial production, and widely used in the daily chemical field. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 It is a physical diagram of Example 9.

[0032] Figure 2 It is a chicken embryo chorioallantoic membrane test diagram of Examples 1, 2, 12 and Comparative Examples 1-4.

[0033] Figure 3 It is a zebrafish test diagram of Examples 16 and Comparative Examples 9-11. SPECIFIC IMPLEMENTATION METHODS

[0035] The technical solutions of the present invention will be further described below in conjunction with the examples. However, it should not be limited thereto. Those that are equivalent substitutions for the technical solutions of the present invention should also be within the protection scope. In addition, adding other active ingredients to the protection solution of the present invention is a well-known technical means in the art and should also be within the protection scope. Unless otherwise specified, the devices and equipment used in the following examples are all conventional devices and equipment in the art. Unless otherwise specified, the raw materials used in the following examples can be obtained commercially. Unless otherwise specified, the technical means used in the following examples are all well-known technical means in the art.

[0036] In the present invention, the polylysine is purchased from Wuxi Plexin Biotechnology Co., Ltd.

[0037] Example 1

[0038] Add 0.1 part of bisabolol to 10 parts of propylene glycol, heat up to 40 °C, and stir until completely dissolved; add 20 parts of polylysine (degree of polymerization 8-20) to 70 parts of water, stir until completely dissolved, and then mix it with the previous solution, adjust the pH to 7, and continue to stir until uniform to obtain Fermented antidandruff agent composition 1 .

[0039] Example 2 (different ratio from 1)

[0040] Add 1 part of bisabolol to 40 parts of propylene glycol, heat up to 40 °C, and stir until completely dissolved; add 5 parts of polylysine (polymerization degree 8 - 20) to 45 parts of water, stir until completely dissolved, then mix it with the aforementioned solution, adjust the pH to 7, and continue stirring until homogeneous to obtain Fermented antidandruff agent composition 2 .

[0041] Example 3 (different polylysine polymerization degree compared with 1)

[0042] Add 0.1 part of bisabolol to 10 parts of propylene glycol, heat up to 40 °C, and stir until completely dissolved; add 20 parts of polylysine (polymerization degree 8 - 30) to 70 parts of water, stir until completely dissolved, then mix it with the aforementioned solution, adjust the pH to 7, and continue stirring until homogeneous to obtain Fermented antidandruff agent composition 3 .

[0043] Example 4 (different polylysine polymerization degree compared with 1)

[0044] Add 0.1 part of bisabolol to 10 parts of propylene glycol, heat up to 40 °C, and stir until completely dissolved; add 20 parts of polylysine (polymerization degree 30 - 40) to 70 parts of water, stir until completely dissolved, then mix it with the aforementioned solution, adjust the pH to 7, and continue stirring until homogeneous to obtain Fermented antidandruff agent composition 4 .

[0045] Example 5 (different soothing agent, optimized ratio compared with 1)

[0046] Add 0.3 part of hydroxyphenylpropionamide benzoic acid to 30 parts of butylene glycol, heat up to 50 °C, and stir until completely dissolved; add 20 parts of polylysine (polymerization degree 8 - 20) to 50 parts of water, stir until completely dissolved, then mix it with the aforementioned solution, adjust the pH to 5.5, and continue stirring until homogeneous to obtain Fermented antidandruff agent composition 5 .

[0047] Example 6 (different soothing agent, optimized ratio compared with 3)

[0048] Add 0.3 part of hydroxyphenylpropionamide benzoic acid to 30 parts of butylene glycol, heat up to 50 °C, and stir until completely dissolved; add 20 parts of polylysine (polymerization degree 8 - 30) to 50 parts of water, stir until completely dissolved, then mix it with the aforementioned solution, adjust the pH to 5.5, and continue stirring until homogeneous to obtain Fermented antidandruff agent composition 6 .

[0049] Example 7 (different soothing agent, optimized ratio compared with 4)

[0050] Add 0.3 parts of hydroxyphenylpropanamide benzoic acid to 30 parts of butanediol, heat up to 50 °C, and stir until completely dissolved; add 20 parts of polylysine (degree of polymerization 30 - 40) to 50 parts of water, stir until completely dissolved, then mix it with the aforementioned solution, adjust the pH to 5.5, and continue stirring until uniform to obtain Fermented antidandruff agent composition 7 。

[0051] Example 8 (Combination of polylysine degrees of polymerization compared with 5)

[0052] Add 0.3 parts of hydroxyphenylpropanamide benzoic acid to 30 parts of butanediol, heat up to 50 °C, and stir until completely dissolved; add 10 parts of polylysine (degree of polymerization 8 - 20) and 10 parts of polylysine (degree of polymerization 8 - 30) to 50 parts of water, stir until completely dissolved, then mix it with the aforementioned solution, adjust the pH to 5.5, and continue stirring until uniform to obtain Fermented antidandruff agent composition 8 。

[0053] Example 9 (Combination of polylysine degrees of polymerization compared with 5)

[0054] Add 0.3 parts of hydroxyphenylpropanamide benzoic acid to 30 parts of butanediol, heat up to 50 °C, and stir until completely dissolved; add 10 parts of polylysine (degree of polymerization 8 - 20) and 10 parts of polylysine (degree of polymerization 30 - 40) to 50 parts of water, stir until completely dissolved, then mix it with the aforementioned solution, adjust the pH to 5.5, and continue stirring until uniform to obtain Fermented antidandruff agent composition 9 。

[0055] Example 10 (Optimization of the combination ratio of polylysine degrees of polymerization compared with 8)

[0056] Add 0.3 parts of hydroxyphenylpropanamide benzoic acid to 30 parts of butanediol, heat up to 50 °C, and stir until completely dissolved; add 5 parts of polylysine (degree of polymerization 8 - 20) and 15 parts of polylysine (degree of polymerization 8 - 30) to 50 parts of water, stir until completely dissolved, then mix it with the aforementioned solution, adjust the pH to 5.5, and continue stirring until uniform to obtain Fermented antidandruff agent composition 10 。

[0057] Example 11 (Optimization of the combination ratio of polylysine degrees of polymerization compared with 8)

[0058] Add 0.3 parts of hydroxyphenylpropanamide benzoic acid to 30 parts of butanediol, heat up to 50 °C, and stir until completely dissolved; add 15 parts of polylysine (degree of polymerization 8 - 20) and 5 parts of polylysine (degree of polymerization 8 - 30) to 50 parts of water, stir until completely dissolved, then mix it with the aforementioned solution, adjust the pH to 5.5, and continue stirring until uniform to obtain Fermented antidandruff agent composition 11 。

[0059] Example 12 (Optimization of the combination ratio of polylysine degrees of polymerization compared with 9)

[0060] Add 0.3 parts of hydroxyphenylpropanamide benzoic acid to 30 parts of butanediol, heat up to 50 °C, and stir until completely dissolved; add 15 parts of polylysine (degree of polymerization 8 - 20) and 5 parts of polylysine (degree of polymerization 30 - 40) to 50 parts of water, stir until completely dissolved, then mix it with the aforementioned solution, adjust the pH to 5.5, and continue stirring until homogeneous to obtain Fermented antidandruff agent composition 12 .

[0061] Example 13 (Optimization of the combination ratio of polylysine degrees of polymerization compared with 9)

[0062] Add 0.3 parts of hydroxyphenylpropanamide benzoic acid to 30 parts of butanediol, heat up to 50 °C, and stir until completely dissolved; add 5 parts of polylysine (degree of polymerization 8 - 20) and 15 parts of polylysine (degree of polymerization 30 - 40) to 50 parts of water, stir until completely dissolved, then mix it with the aforementioned solution, adjust the pH to 5.5, and continue stirring until homogeneous to obtain Fermented antidandruff agent composition 13 .

[0063] Comparative Example 1 (Compared with Example 1, without alcohol and soothing agent)

[0064] Add 20 parts of polylysine (degree of polymerization 8 - 20) to 70 parts of water, stir until completely dissolved, then adjust the pH to 7, and continue stirring until homogeneous to obtain Composition 1 .

[0065] Comparative Example 2 (Compared with Example 2, different polylysine)

[0066] Add 1 part of bisabolol to 40 parts of propylene glycol, heat up to 40 °C, and stir until completely dissolved; add 5 parts of polylysine (commercially available, Adamas life, MW: 3500 - 4600, degree of polymerization about 25 - 35) to 45 parts of water, stir until completely dissolved, then mix it with the aforementioned solution, adjust the pH to 7, and continue stirring until homogeneous to obtain Composition 2 .

[0067] Comparative Example 3 (Compared with Example 12, without alcohol and soothing agent)

[0068] Add 15 parts of polylysine (degree of polymerization 8 - 20) and 5 parts of polylysine (degree of polymerization 30 - 40) to 50 parts of water, stir until completely dissolved, then mix it with the aforementioned solution, adjust the pH to 5.5, and continue stirring until homogeneous to obtain Composition 3 .

[0069] Comparative Example 4 (Compared with Example 12, different polylysine)

[0070] Add 0.3 parts of hydroxyphenylpropionamide benzoic acid to 30 parts of butanediol, heat up to 50 °C, and stir until completely dissolved; add 20 parts of polylysine (commercially available, Adamas life, MW: 3500 - 4600, degree of polymerization about 25 - 35) to 50 parts of water, stir until completely dissolved, then mix it with the aforementioned solution, adjust the pH to 5.5, and continue stirring until homogeneous to obtain Composition 4 .

[0071] According to the anti-(or inhibitory)-bacterial experiment in the "Disinfection Technical Specification (2002 Edition)", use 5 mm test pieces to test the antibacterial effect of the above solution (the selected bacteria are Malassezia, Propionibacterium, and Staphylococcus epidermidis), and judge whether it has antibacterial properties according to the diameter of the antibacterial zone. The results are listed in Table 1.

[0072] Judgment of the antibacterial effect of the samples in Table 1:

[0073] If the diameter of the antibacterial zone is greater than 7 mm, it is judged to have antibacterial effect (√); if the diameter of the antibacterial zone is between 5 - 7 mm, it is judged to have weak antibacterial effect (△); if the diameter of the antibacterial zone is less than or equal to 7 mm, it is judged to have no antibacterial effect (×).

[0074] According to the relevant requirements of the "Chorioallantoic Membrane Test of Chicken Embryos for Eye Irritation / Corrosion of Cosmetics (SN / T 2329 - 2009)", conduct irritation scoring, and the results are listed in Table 1.

[0075] Irritation scoring method:

[0076]

[0077] Table 1

[0078]

[0079]

[0080] Based on the above data, by comparing Example 1 with Examples 3 and 4, Example 2 with Comparative Example 2, and Example 12 with Comparative Example 4, it is found that polylysine in the high polymerization degree range of 30 - 40 has a better antibacterial effect on bacteria, especially Staphylococcus epidermidis. However, polylysine with a relatively low polymerization degree range (8 - 20, 8 - 30) can better inhibit Malassezia. Through the corresponding ratio combinations of the polylysine of the present invention, a composition with excellent antibacterial effects on Malassezia, Propionibacterium, and Staphylococcus epidermidis that may exist on the human scalp can be obtained. By comparing Example 1 and Example 2, it can be seen that when the relative ratio of propylene glycol to water in the system increases, even if a soothing agent is added, the irritation of the composition still slightly increases, which is mainly due to the safety of propylene glycol itself. The overall irritation of Examples 1 - 4 is less than that of Examples 5 - 13 because the pH of the compositions in Examples 1 - 4 is overall neutral. From Examples 5 - 13, it can be concluded that polylysine with a small polymerization degree has less irritation. By comparing Example 1 with Comparative Example 1 and Example 12 with Comparative Example 3, it can be seen that the soothing components in the fermented anti-dandruff agent composition of the present invention can effectively reduce the irritation brought by the composition to the skin.

[0081] The human skin is slightly acidic. Example 12 of the present invention has obvious antibacterial properties against Malassezia and Propionibacterium that cause dandruff, and can maintain slight irritation under acidic conditions.

[0082] Example 14

[0083] Apply that of Example 1 Fermented antidandruff agent composition 1 to shampoo, and the specific formula is shown in Table 2.

[0084] Example 15

[0085] Apply that of Example 2 Fermented antidandruff agent composition 2 to shampoo, and the specific formula is shown in Table 2.

[0086] Example 16

[0087] Apply that of Example 12 Fermented antidandruff agent composition 12 to shampoo, and the specific formula is shown in Table 2.

[0088] Comparative Example 5

[0089] Apply that of Comparative Example 1 Composition 1 to shampoo, and the specific formula is shown in Table 2.

[0090] Comparative Example 6

[0091] Apply that of Comparative Example 2 Composition 2 to shampoo, and the specific formula is shown in Table 2.

[0092] Comparative Example 7

[0093] Apply that of Comparative Example 3Composition 3 Applied to shampoo, the specific formulation is shown in Table 2.

[0094] Comparative Example 8

[0095] The one in Comparative Example 4 Composition 4 Applied to shampoo, the specific formulation is shown in Table 2.

[0096] Comparative Example 9

[0097] Apply piroctone olamine (OCT) to shampoo, the specific formulation is shown in Table 2.

[0098] Comparative Example 10

[0099] Apply zinc pyrithione (ZPT) to shampoo, the specific formulation is shown in Table 2.

[0100] Comparative Example 11

[0101] Apply selenium disulfide to shampoo, the specific formulation is shown in Table 2.

[0102] Table 2 Shampoo formulation (%)

[0103]

[0104] According to the suspension quantitative method in "QB / T 2738-2012 Evaluation Method for Antibacterial and Bacteriostatic Effects of Daily Chemical Products", the antibacterial effects of Examples 14-16 and Comparative Examples 5-11 were evaluated, and their antibacterial effects against Malassezia were tested. The results are listed in Table 3.

[0105] According to the relevant requirements of "GB / T 21808-2008 14-Day Test for Prolonged Toxicity of Chemicals to Fish", the soothing effect was evaluated, and the formation number of neutrophils in the abdomen and tail of zebrafish was analyzed. The concentration of the solution in the model control group was 0.16 mg / L of copper sulfate solution. The relevant test results are listed in Table 3.

[0106] Table 3

[0107] Sample Bacteriostatic rate (acting for 20 min) Neutrophil inhibition rate Example 14 82.46％ 28.67％ Example 15 80.22％ 29.43％ Example 16 85.44％ 29.07％ Comparative example 5 81.96％ 36.46％ Comparative example 6 54.96％ 30.33％ Comparative example 7 85.04％ 38.50％ Comparative example 8 48.71％ 31.26％ Comparative example 9 71.45％ 37.90％ Comparative example 10 61.35％ 49.59％ Comparative example 11 89.51％ 60.60％

[0108] From the data analysis, by comparing Example 14 with Comparative Example 5, and Example 16 with Comparative Example 8, it can be seen that the shampoo of the composition of the present invention has a more excellent antibacterial rate; by comparing Examples 14-16 with Comparative Examples 9-11, it can be seen that at the same addition amount, the antibacterial rate of the fermented antidandruff agent composition of the present invention against Malassezia is higher than that of the commercially available OCT and ZPT antidandruff agents, but slightly weaker than selenium disulfide. By comparing Example 13 with Comparative Example 5, Example 16 with Comparative Example 7, and the shampoos of Example 16 and Comparative Examples 9-11 added with the commercially available antidandruff agent in the same proportion, the number of neutrophils formed in the abdomen and tail of zebrafish of the shampoo added with the fermented antidandruff agent composition of the present invention is more, that is, the inhibitory rate of neutrophils in zebrafish embryos is lower, and it has a better soothing effect. Especially for selenium disulfide, under the condition of little difference in antibacterial rate, the composition of the present invention greatly increases the soothing efficacy of the product and reduces the irritation to the scalp.

Claims

1. A fermentation anti-dandruff agent composition, characterized in that, The fermented anti-dandruff agent composition is composed of polylysine, a soothing agent, alcohols and water.

2. The fermentation anti-dandruff agent composition according to claim 1, characterized in that, The polylysine is obtained by a microbial method.

3. The fermentation anti-dandruff agent composition according to claim 1 or 2, characterized in that, The polymerization degree range of the polylysine is specifically divided into 8 - 20, 8 - 30, 30 - 40, 8 - 20 and 8 - 30, 8 - 20 and 30 - 40.

4. The fermented antidandruff agent composition according to claim 1, characterized in that, The soothing agent is one or a combination of two or more of bisabolol, trans - 4 - tert - butylcyclohexanol, hydroxyphenylpropionamide benzoic acid, Wolfiporia cocos sclerotium extract, Centella asiatica extract, Piper wattii root extract, Sophora flavescens extract, Portulaca oleracea extract.

5. The fermented anti-dandruff agent composition according to claim 1, characterized in that, The alcohols are one or a combination of two or more of isopropanol, propylene glycol, butylene glycol, 1,2 - pentanediol, hexylene glycol, polyethylene glycol, polypropylene glycol.

6. The fermentation anti-dandruff agent composition according to claim 1, wherein, The mass ratio of polylysine, the soothing agent, alcohols and water is (5 - 30):(0.1 - 1):(10 - 40):(45 - 70).

7. A preparation method of a fermented antidandruff agent composition, characterized in that, The preparation method includes the following steps: (1) Mix and stir the soothing agent and alcohols according to the ratio until completely dissolved; (2) Add polylysine to water and stir until completely dissolved; (3) Mix the solutions of steps (1) and (2) and continue to stir until uniform to obtain the fermented anti - dandruff agent composition.

8. The preparation method of a fermented antidandruff agent composition according to claim 7, characterized in that, In step (1), the temperature is maintained at 40 - 50 °C; in step (3), the pH of the solution system needs to be maintained at 4 - 9.

9. A fermentation anti-dandruff agent composition according to any one of claims 1-6, or a fermentation anti-dandruff agent composition prepared by the preparation method according to any one of claims 7-8, characterized in that, The fermented anti - dandruff agent composition has an obvious inhibitory effect on Malassezia.

10. Use of a fermented anti-dandruff agent composition according to any one of claims 1-6 or a fermented anti-dandruff agent composition prepared by the preparation method according to any one of claims 7-8, characterized in that, The fermented anti - dandruff agent composition is applied to anti - dandruff products.

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