104 results about "Clematis" patented technology

The invention provides a traditional Chinese medicine gel patch for treating gouty arthritis and a preparation method thereof. The traditional Chinese medicine gel patch for treating gouty arthritis comprises at least a gel layer loaded with traditional Chinese medicine extracts; the traditional Chinese medicine extracts comprise the following raw materials: coix seed, phellodendron amurense, clematis root, radix scutellariae, atractylodes lancea, radix ophiopogonis, radix scutellariae, acer scutellariae, radix scutellariae, radix aconiti kusnezoffii, radix strychnii, frankincense, myrrh, angelica sinensis, safflower, cyathula root, radix paeoniae rubra, radix smilacis gracile, radix striatae, eucalyptus glomeratae, sophora flavescens, andrographis paniculatae, radix lobeliae, rhizoma coptidis, radix scutellariae, cassia twig, zanthoxylum bungeanum, and borneol; and a matrix of the gel layer comprises the following raw materials in parts by weight: silk fibroin, polyvinyl pyrrolidone, azido-polyethylene glycol-carboxylic acid, 1-ethynylcyclopropylcarboxylic acid, 2,5-dicarboxylic acid-3,4-ethylenedioxythiophene, and glycerol. The traditional Chinese medicine gel plaster of the present invention can achieve good therapeutic effects through percutaneous absorption.

The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine composition for relieving pain, promoting blood circulation, diminishing swelling and removing blood stasis, application thereof and a blood stasis dispersing and pain relieving patch. The invention provides a traditional Chinese medicine composition for relieving pain, activating blood, diminishing swelling and removing stasis. The traditional Chinese medicine composition comprises the following components: lycopodium clavatum, radix clematidis, asarum, gentiana macrophylla, radix angelicae, rheum officinale, notopterygium root, roasted herba epimedii, radix achyranthis bidentatae, peach kernel, radix angelicae pubescentis, vinegar-processed rhizoma corydalis, vinegar-processed rhizoma cyperi, moutan bark, prepared kusnezoff monkshood root, prepared monkshood, vinegar-processed frankincense, Sinkiang lithospermum, vinegar-processed myrrh and sanguisorba officinalis. The blood stasis dispersing and pain relieving patch has the advantages of being good in curative effect, quick in effect taking, convenient to use, safe and reliable, can be used for treating traumatic injuries and pain caused by cervical spondylosis, scapulohumeral periarthritis, lumbar disc herniation, lumbar vertebrae hyperosteogeny, spinal stenosis, sciatica, ankylosing spondylitis, arthritis and other diseases, and can achieve the effect of treating both symptoms and root causes.

The invention provides a traditional Chinese medicine gel emplastrum for treating gouty arthritis and a preparation method thereof, the traditional Chinese medicine gel emplastrum for treating gouty arthritis at least comprises a gel layer loaded with a traditional Chinese medicine extract, the traditional Chinese medicine extract comprises the following raw materials: semen coicis, cortex phellodendri, radix clematidis, kadsura coccinea, rhizoma atractylodis, rhizoma dioscoreae hypoglaucae, red psychotria leaf, alangium platanifolium, kadsura pepper stem, radix aconiti, radix aconiti agrestis, semen strychni, frankincense, myrrh, angelica sinensis, safflower carthamus, radix cyathulae, radix paeoniae rubra, rhizoma smilacis glabrae, lycopodium clavatum, radices hemerocallis, weeping willow twig, evodia lepta, swamp mahogany, radix sophorae flavescentis, herba andrographitis, Chinese lobelia, coptis chinensis, edible tulip, cassia twig, pepper and borneol; a matrix of the gel layer is prepared from the following raw materials in parts by weight: silk fibroin, polyvinyl pyrrolidone, azide-polyethylene glycol-carboxylic acid, 1-ethynyl cyclopropyl carboxylic acid, 2, 5-dicarboxylic acid-3, 4-ethylenedioxythiophene and glycerol. The traditional Chinese medicine gel emplastrum disclosed by the invention can achieve a good treatment effect through percutaneous absorption.

This invention discloses a muscle-relaxing and meridian-clearing health powder and its preparation and application methods, belonging to the technical field of traditional Chinese medicine external health care products. The health powder is composed of fifteen raw medicinal materials: safflower, angelica, asarum, kudzu root, red peony root, scutellaria, dragon's blood, saposhnikovia root, frankincense, myrrh, cinnamon twig, mugwort leaf, coptis root, *Lycopodium clavatum*, and *Clematis armandii*. These materials are dried, pulverized to 80-120 mesh, and then evenly mixed. This invention, through a unique formulation design, integrates multiple effects such as promoting blood circulation and removing blood stasis, dispelling wind and cold, relieving muscle tension and clearing meridians, and clearing heat and drying dampness. Combined with a refined pulverization process, it significantly improves the dissolution rate and transdermal absorption efficiency of the drugs. During use, a hot compress applied via steam allows the medicinal properties to directly reach the affected area, effectively relieving pain and discomfort in the neck, shoulders, waist, legs, and joints. This invention's product has a scientific formulation, advanced technology, is convenient to use, and highly safe, possessing broad market application prospects.

The application discloses a mixed biological bacterial fertilizer, a preparation method and application thereof. The application is based on the three major nutrient elements of nitrogen, phosphorus and potassium which are necessary for plant growth, and eight kinds of bacteria are compounded, including two kinds of phosphorus solubilizing bacteria, i.e., soil olive-shaped bacteria and protomonospora; three kinds of potassium solubilizing bacteria, i.e., Cabriales Bacillus, Siamese Bacillus and Beiles Bacillus; and three kinds of nitrogen-fixing bacteria, i.e., lemon protomonospora, Yunnan protomonospora and Korean pseudomonas. The mixed biological bacterial fertilizer is prepared by combining the eight kinds of growth-promoting bacteria with kitchen garbage and biomass waste, and meets the growth demand of plants. The sunflower, clematis and sunflower are planted in pots. The bacterial fertilizer has the advantages of fast germination, high germination rate, fast growth and healthy and dense leaves. The biological organic fertilizer has the advantages of green and environmental protection, and lays a theoretical foundation for replacing traditional chemical fertilizer with the biological organic fertilizer.

The invention discloses a method for distinguishing clematis palustris from other clematis plants and application of the method. The method for distinguishing the clematis from the other clematis plants comprises the following steps: detecting whether the genotype of 201st deoxyribonucleotide in a sequence 1 of a plant to be detected is an AA genotype or a CC genotype by adopting a primer pair consisting of a single-stranded DNA (Deoxyribonucleic Acid) shown in a sequence 2 and a single-stranded DNA shown in a sequence 3; the to-be-detected plant is clematis hispida; if the genotype of the plant to be detected is AA genotype, the plant to be detected is other clematis plants. Experiments prove that the method provided by the invention can be used for effectively distinguishing the clematis palustris from other 14 clematis plants, and an important tool is provided for quickly and accurately identifying the clematis palustris in the seedling stage.

The invention discloses a traditional Chinese medicine composition and a traditional Chinese medicine preparation for treating sleep disorder caused by new coronavirus infection and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines. Aiming at the problems that no medicine with definite curative effect exists for sleep disorder of a new coronavirus infected patient at present, and the medicine can only depend on the individual immunity of the patient to resist, the traditional Chinese medicine composition comprises the following traditional Chinese medicinal materials in parts by weight: 10g of radix pseudostellariae, 12g of radix paeoniae rubra, 10g of radix salviae miltiorrhizae, 6g of rhodiola rosea, 6g of exocarpium citri grandis, 5g of ginger processed pinellia, 15g of poria cocos, 15g of poria with hostwood, 15g of herba patriniae, 15g of tuber fleeceflower stem, 12g of hairyvein agrimony, 6g of liquorice, 10g of chrysanthemum and 6g of cassia twig. The traditional Chinese medicine composition is based on the principles of tonifying qi and activating blood, reducing phlegm and dredging collaterals, and clearing pestilence and soothing nerves, all the medicines are synergistic and supplement one another, the blood oxygen concentration and the red blood cell oxygen carrying capacity of a human body are improved, the blood viscosity is reduced, microcirculation is promoted, residual toxins are eliminated, and patients are helped to recover high-quality sleep.

The invention belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a construction method of a specific chromatogram of caulis clematidis armandii or derivative products thereof. The invention provides a construction method of a characteristic chromatogram of caulis clematidis armandii or a derivative product thereof, which comprises the following steps: by taking octadecylsilane chemically bonded silica as a filler and a mobile phase comprising a formic acid-containing aqueous solution and acetonitrile, carrying out a specific gradient elution procedure to obtain 22 common characteristic peaks, and realizing effective separation of the common characteristic peaks, so that the separation degree is good, and the accuracy is high. The obtained characteristic spectrum has more characteristic peaks and better baseline separation, and is easier to position and qualitatively analyze; the precision, the stability and the repeatability are relatively good; compared with the prior art, the method has the advantages that the characteristic peaks are more, six characteristic components including the straight clematis seed B, the lariciresinol, the secoisolariciresinol, the lariciresinol-4-O-beta-D-glucoside, the caffeic acid and the ferulic acid are identified, and a basis is provided for quality detection and content determination of the caulis clematidis armandii or derivative products of the caulis clematidis armandii.

The invention relates to a CllaF3 '5' H gene and application thereof. The CllaF3 '5' H gene comprises a nucleotide sequence as shown in SEQ ID NO: 1. The CllaF3 '5' H protein comprises a protein coded by the CllaF3 '5' H gene. According to the invention, a CllaF3 '5' H gene is screened and cloned from clematis lanceolata, and the correlation between the CllaF3 '5' H gene and the content of flavonoid metabolites such as delphinidin is found through research; stable germplasm with blue flower potential can be formed by utilizing the gene.

1. The name of the design product: Yangqin (with summer wisteria purple). 2. The use of the design product: mainly used for musical instruments. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

This invention discloses a natural plant-extracted biological insecticide and its preparation and application methods, belonging to the field of agricultural biotechnology. The insecticide uses cypress leaves, stemona root, clematis root, pinellia root, and ailanthus leaves as raw materials, prepared through boiling, cooling, filtration, sealed maturation, double-barrel water bath temperature gradient concentration, and pasteurization. This insecticide kills pests by sealing their spiracles and rapidly penetrating their bodies, causing suffocation. It primarily works through contact, exhibiting good residual effect, low resistance development, no chemical residues, and is environmentally friendly. The preparation process is mild, maximizing the retention of heat-sensitive active ingredients. The product is highly stable and low-cost, particularly suitable for controlling Diptera, Coleoptera, Orthoptera, Hemiptera, and / or Lepidoptera pests.

The invention discloses a processing method of stems and leaves of pilea japonica and pilea japonica scented tea. The method comprises the following steps: harvesting stems and leaves 10 cm above the ground in the earlier stage of flowering; after cleaning and slitting, carrying out two-stage fixation at 70 DEG C and 50 DEG C; softening at the temperature of 40 DEG C and the RH of 70%, and adding the wolfberry powder and the ginger juice; carrying out spraying treatment for 5 minutes by using a 0.1% cellulase solution, and rolling by using a silica gel coated roller until the wall breaking rate is 80%; heating and drying at the temperature of 50 DEG C, 55 DEG C and 45 DEG C until the water content is 8 + / -0.5%; and finally, spraying vitamin C or chitosan to form a film, and carrying out vacuum nitrogen-filled packaging. The retention rate of flavone and polysaccharide of the prepared eriocheir sinensis flower tea is larger than or equal to 92%, the retention rate of volatile oil is larger than or equal to 90%, the leaching rate is increased by 30% or above, the retention rate of main components is larger than or equal to 90% after the eriocheir sinensis flower tea is stored for 24 months at normal temperature, and the problems that in the prior art, the retention rate of components is low, leaching is difficult, and the storage period is short are solved.

The invention discloses an InDel molecular marker for identifying clematis gossypii and application of the InDel molecular marker. The InDel molecular marker is composed of a single-stranded DNA as shown in a sequence 1 and a single-stranded DNA as shown in a sequence 2. The InDel molecular marker is used for carrying out PCR (Polymerase Chain Reaction) amplification on 15 clematis plants and carrying out capillary electrophoresis detection on a PCR product to find that the InDel molecular marker has two banding patterns of 191bp and 187bp in the 15 clematis plants, but only has the banding pattern of 187bp in clematis cotton, and the banding patterns in other 14 clematis plants are all 191bp banding patterns. Therefore, in practical application, the InDel molecular marker can be used for identifying the clematis gossypii, and particularly, rapid and accurate identification of the clematis gossypii can be realized in the seedling stage. The method disclosed by the invention has important significance on identification, research and utilization of clematis gossypii.

The invention relates to a traditional Chinese medicine liquid and a traditional Chinese medicine ointment for expelling toxins from osteoporosis and a preparation method thereof. The traditional Chinese medicine liquid for expelling toxins from osteoporosis is prepared from the following raw materials in parts by weight: 10-15 parts of radix clematidis, 12-18 parts of trifid sopubia herb, 10-15 parts of garden balsam stem, 15-25 parts of suberect spatholobus stem, 6-10 parts of safflower, 9-12 parts of Chinese angelica, 6-8 parts of liquorice and 8-12 parts of folium artemisiae argyi. The preparation method of the bone seam detoxifying traditional Chinese medicine liquid comprises the following steps: a, pretreating the raw materials; b, decocting and extracting; c, filtering and concentrating; d, blending and homogenizing; and e, sterilizing and packaging. The traditional Chinese medicine liquid and the traditional Chinese medicine ointment for detoxifying the bone seams are particularly used for detoxifying toxins such as deep damp turbidity and phlegm stasis in the bone seams, and are suitable for relieving symptoms such as ankylosis, deep pain, hyperostosis and the like caused by deposition of toxins in the bone seams. The bone seam detoxification traditional Chinese medicine liquid and the traditional Chinese medicine ointment have the remarkable advantages of being high in seepage force, comprehensive in detoxification, high in safety, wide in applicability and the like.

The present invention discloses a composition for preventing and treating sclerotinia rot and black rot of cauliflower and a method for using the composition. The composition comprises the following components: 65-85 parts of a clematis extract, 50-70 parts of a lemongrass extract, 1-3 parts of organosilicon, 800-1200 parts of xanthan gum, and 800-1200 parts of water. The clematis extract, the lemongrass extract, the organosilicon, and the xanthan gum are added to water and stirred evenly to obtain a medicament. On a sunny day, a portion of a flower branch is cut from top to bottom with a sharp knife at an incision angle of 10 to 20 degrees. The obtained medicament is evenly applied to the incision with a brush. The medicament can significantly reduce the incidence of sclerotinia rot and black rot of the cauliflower after the flower branch is cut.

The invention discloses an SNP (Single Nucleotide Polymorphism) site for distinguishing Clematis brevicaulis from other Clematis plants and application of the SNP site. The name of the SNP site is CLA25318-11, the SNP site is located at the 305th deoxyribonucleotide of SEQ ID NO: 1, the polymorphism of the SNP site is C / A, and the SNP site has two genotypes: CC and AA genotypes. The invention also discloses a PCR (Polymerase Chain Reaction) primer pair for detecting the genotype of the SNP locus. By adopting the PCR primer pair to carry out PCR amplification on genomic DNA of 15 clematis plants, it is found that only the short-column clematis is of a CC genotype, and the other clematis plants are of an AA genotype. Therefore, the clematis brevituba and other 14 clematis plants can be effectively distinguished by utilizing the SNP site provided by the invention. The invention provides a powerful tool for early accurate identification of the clematis brevicaulis and systematic taxonomic status research of the clematis brevicaulis.

The application discloses a method for improving the cutting survival rate of Adenium obesum, which is composed of steps of cutting selection, cutting time, cutting method, preparation before cutting, cutting and post-stage management, and effectively solves the problems of low rooting rate and uneven growth of traditional cutting, so that the survival rate reaches more than 70%, the method is simple, convenient to operate and low in cost, and is suitable for practical production application. The method can reduce the digging of wild Adenium obesum, provide good plant materials for the fields of landscape engineering and family gardening, and provide stable, rich medicinal resources and technical support for the Chinese medicine industry.

The present application relates to a kind of compound preparation for preventing and treating poultry enteritis diarrhea, it is mainly made of the following weight parts proportion raw materials: catnip 10-12 parts, 10-12 parts of banlangen, 8-10 parts of heartleaf hedyotis, 10-12 parts of pepper, 10-15 parts of green wormwood, 8-10 parts of Chinese clematis, 10-12 parts of atractylodes, 3-5 parts of xylo-oligosaccharide, 1-3 parts of garlicin, 1-3 parts of capsaicin, 5-7 parts of vitamin C, 0.1-0.3 parts of vitamin A, 4-5 parts of bacillus licheniformis, 4-5 parts of butyric acid clostridium, 10-12 parts of brown sugar.The compound preparation can effectively improve the immune function of organism, prevent and treat diarrhea caused by poultry enteritis, improve intestinal health;While preparation enters organism, is easily absorbed, and clinical administration is convenient, suitable for group administration, save manpower and material resources.

1. The name of the design product: pendant (double-petaled Clematis Q version character key chain). 2. The use of the design product: used for pendant decoration, and to promote the unique Chinese herbal medicine, double-petaled Clematis. 3. The design points of the design product: the combination of shape, pattern and color. 4. The picture or photo that best indicates the design points: front view. 5. The design for which protection is sought includes color. 6. The design product is a thin product, and the left view, right view, top view and bottom view are omitted.

The invention discloses traditional Chinese medicine external medicinal liquor for treating bone diseases and a preparation method and application thereof. The medicinal liquor is prepared from, by weight, 30 g of ginseng under forest, 60 g of raw radix astragali, 90 g of dragon's blood, 60 g of sargentgloryvine stem, 30 g of radix clematidis, 60 g of radix angelicae, 30 g of scorpion, 60 g of lumbricus, 60 g of dried river crab, 30 g of silkworm larva, 60 g of ground beetle, 30 g of frankincense, 30 g of myrrh, 60 g of teasel root and 60 g of rhizoma drynariae. The traditional Chinese medicine is prepared from 60 g of native copper, 30 g of amber, 30 g of pericarpium zanthoxyli, 25 g of radix aconiti, 25 g of radix aconiti agrestis, 60 g of caulis spatholobi, 30 g of cortex erythrinae, 45 g of semen strychni, 15 g of musk and 30 g of borneol. The medicinal liquor achieves the effects of rapidly relieving swelling and pain, promoting bone healing, dispelling wind and eliminating dampness and the like through the synergistic effect of multiple targets, is especially suitable for arthralgia type osteoarticular diseases and traumatic injuries, and has remarkable curative effects on osteoproliferation, fracture recovery and the like and high safety according to clinical cases.

A new Clematis plant, particularly distinguished by abundant purple and pink flowers on old growth; double flowers produced on new growth; and compact growth with short internodes, is disclosed.

The invention discloses an application of an SNP (Single Nucleotide Polymorphism) site CLA25328 and a detection primer pair of the SNP site CLA25328 in identifying clematis hispida. The SNP site CLA25328 is located at the 182nd site of SEQ ID NO: 1, the polymorphism of the SNP site is C / T, and two genotypes of CC and TT coexist. The detection primer pair is composed of DNA molecules as shown in SEQ ID NO: 2 and SEQ ID NO: 3. PCR amplification and first-generation sequencing are carried out on genomic DNA of 15 clematis plants by adopting the primer pair, and it is found that only clematis angulata is of a TT genotype, and the other clematis plants are of a CC genotype. Therefore, the clematis hispida and other 14 clematis plants can be effectively distinguished by utilizing the SNP site and the detection primer pair provided by the invention. The method disclosed by the invention plays an important role in resource census, efficient evaluation of population genetic diversity and rapid identification of a seedling stage of clematis hispida.

The invention discloses a novel method for extracting and concentrating efficient components of a kidney-tonifying Juanbi pill, which comprises the following steps: pretreating medicinal materials: taking prescription medicinal materials of the kidney-tonifying Juanbi pill; the traditional Chinese medicine composition is prepared from rhizoma drynariae, prepared rehmannia root, Chinese angelica, paniculate swallowwort root, ground beetle, stiff silkworm (stir-fried with bran), centipede, scorpio, honeycomb (stir-fried with clear water), lumbricus (processed with wine), zaocys dhumnade (processed with wine), corydalis tuber, pyrola, epimedium, berba aristolochiae mollissimae, geranium wilfordii, caulis spatholobi, scandent hop, radix rehmanniae recen and polygonum cuspidatum. Through targeted medicinal material pretreatment and ultrasonic-assisted extraction, the problems of slow dissolution of plant medicinal effective components and difficult extraction of animal medicinal cuticle of the kidney-tonifying Juanbi pill are effectively solved, and the extraction rate of the medicinal effective components stably reaches 60% or above. Effective components of medicinal materials such as polygonum cuspidatum, caulis spatholobi, berba aristolochiae mollissimae, geranium wilfordii and rehmannia in the prescription can be fully dissolved out, and waste of raw materials is avoided.

The invention discloses a traditional Chinese medicine external application formula for adjuvant treatment of bone fracture, a pharmaceutical preparation and application, the traditional Chinese medicine external application formula is prepared from the following raw material medicines by weight: radix clematidis, white mulberry root-bark, safflower, radix stephaniae tetrandrae, lycopodium clavatum, beautiful sweetgum fruit, gentiana macrophylla, divaricate saposhnikovia root, angelica sinensis, radix angelicae, pawpaw, vinegar-processed corydalis tuber, poria peel, processed frankincense and processed myrrh. The in-vitro shock wave treatment and the external prescription are used for treating the patient with plantar fasciitis, and compared with single in-vitro shock wave treatment, the combined use of the in-vitro shock wave treatment and the external prescription can better reduce the level of inflammatory factors, relieve pain and accelerate the disappearance of symptoms and signs.

The application belongs to the field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for treating chronic insomnia and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 6-18 parts of mimusops elengi, 6-18 parts of lotus seed, 6-18 parts of lily, 9-30 parts of clematis, and 0.25-1 part of safflower. According to the pathogenesis of yang failing to enter yin in traditional Chinese medicine, the plant medicine with the characteristic of night movement is used for treating insomnia. The clinical curative effect of the traditional Chinese medicine composition presents multilink, can shorten the sleep latency of chronic insomnia patients, prolong the sleep time, reduce the number of night awakenings, improve the daytime function, has no obvious adverse reactions, is high in safety, and is worthy of clinical popularization and application.

The invention discloses a clematis tissue culture method which comprises the following steps: (1) sterilizing a clematis explant, and inoculating the clematis explant to an axillary bud induction culture medium with the morphological lower end upward for culture; (2) inoculating the explant cultured in the step (1) to a hormone-free culture medium for culturing; (3) inoculating the explant cultured in the step (2) to a rooting culture medium for culturing; and (4) domesticating and transplanting the explants cultured in the step (3) to obtain clematis plants. By means of the tissue culture method, high-quality rapid rooting of the clematis explant can be achieved, meanwhile, a complete plant with a thick and strong root system can be effectively induced, weather conditions do not need to be considered, a large number of high-quality clematis seedlings can be produced within a short time, market requirements are met, and great benefits are brought to industrial production.

The invention discloses a field sand storage dormancy breaking technology for clematis grandiflora seeds, which combines gibberellin treatment and field sand storage, significantly improves the germination rate of the clematis grandiflora seeds, and effectively improves the germination ability of the seeds. The seed germination rate reaches 60% or above; the method is simple, convenient to operate, low in cost and suitable for actual production and application. Digging of wild clematis grandiflora can be reduced, good plant materials are provided for urban landscaping in northern cold regions, and stable and rich medicine sources and technical guarantee are provided for the traditional Chinese medicine industry.

This invention discloses a method for distinguishing *Clematis acerifolia* from other *Clematis* species and the KASP primer set used therein. The method for distinguishing *Clematis acerifolia* from other *Clematis* species includes the following steps: detecting the genotype of the deoxyribonucleotide at position 7769 of sequence 1 of the plant to be tested (AA, AG, or GG); and determining whether the plant is *Clematis acerifolia* or another *Clematis* species based on its genotype: if the genotype is AA or AG, the plant is *Clematis acerifolia*; if the genotype is GG, the plant is another *Clematis* species. Using this method and KASP primer set, accurate identification of *Clematis acerifolia* can be achieved at the seedling stage, which is of great significance for the protection of *Clematis acerifolia*.

The invention discloses a method for efficiently extracting and purifying radix clematidis volatile oil, and relates to the technical field of radix clematidis extraction.The method includes the steps that raw materials are prepared, and power equipment and raw materials needed by extraction and purification are prepared in advance; the volatile oil in the radix clematidis is extracted by adopting a supercritical CO2 extraction technology, an optimal extraction process of the volatile oil is screened through a single-factor test and an orthogonal test, and the volatile oil is separated and collected, so that compared with a traditional steam distillation method, the extraction effect is more excellent, and a component with relatively high content in the volatile oil of the radix clematidis is linoleic acid; an important reference basis is provided for research on effective components and pharmacological effects of the radix clematidis volatile oil, radix clematidis volatile oil extraction data and corresponding analysis results are managed, visualized and stored, and the intelligent level of radix clematidis volatile oil extraction and purification management is improved.

The invention discloses an InDel molecular marker for distinguishing or assisting in distinguishing Akebia zhejiangensis from other clematis plants and application of the InDel molecular marker. And the InDel molecular marker is located at the 1163rd-1168th sites of the sequence 3. The invention also discloses a PCR (Polymerase Chain Reaction) primer pair for amplifying the InDel molecular marker, wherein the PCR primer pair is composed of a single-stranded DNA (Deoxyribonucleic Acid) molecule as shown in a sequence 1 and a single-stranded DNA molecule as shown in a sequence 2. Verification on the InDel molecular marker in 15 clematis plants shows that the InDel molecular marker can accurately and effectively distinguish the Zhejiang clematis stem from other 14 clematis plants. The method has important significance on identification of the Zhejiang clematis stem and early classification and identification of the clematis plant.