2 results about "Bromocresol purple" patented technology

The application provides a method for detecting plant growth hormone-like substances in sludge stabilization products, comprising the following steps: contacting the root system of a plant with a sludge extract solution added with CaCl2 to activate a messenger; transferring the plant treated by the activated messenger to an agar gel layer plate added with bromocresol purple indicator; observing the color development of the agar gel layer plate during the culture process; and judging the content of the plant growth hormone-like substances in the sludge extract solution according to the color development. The method for detecting plant growth hormone-like substances in sludge stabilization products provided by the application is based on the acidification degree of the root system of a plant, takes bromocresol purple as an indicator, and combines color development with a plant water culture experiment, so that simple, rapid and low-cost qualitative detection of the plant growth hormone-like substances in sludge stabilization products is realized.

This invention belongs to the field of microbial culture technology. It provides a method for culturing cariogenic acid-producing anaerobic bacteria and its application. The culture medium comprises the following components in the following proportions: peptone 0.5-5%, sucrose 1-10%, sodium chloride 0.1-0.6%, bromocresol green 0.1-0.3%, bromocresol purple 0.01-0.15%, vitamin C 0.01-0.05%, Tween-80 0.01-0.06%, phenylethanol 0.01-0.25%, and deionized water, forming a liquid culture medium. Based on the specific requirements of bacterial culture, this invention is used for culturing bacteria from teeth and the oral cavity. Peptone is used as the nitrogen source, and sucrose as the carbon source. When bacteria multiply rapidly, they convert sucrose into an acidic substance, changing the pH of the culture medium. In the presence of bromocresol green / bromocresol purple, the color of the culture medium changes, allowing for the determination of bacterial content in the sample and the prediction of the risk of dental caries.