45 results about "Bromocresol purple" patented technology

The invention relates to a rice metabolism detecting reagent and method, concretely used in the condition of detecting rice metabolism. It mainly takes and dissolves nitrazine yellow / chlorophenol red, p-nitrophenol, bromocresol green / bromothymol blue / resorcinol blue, bromocresol purple, and hematoxylin / cresol red / bromophenol red / methyl red into alcohol, and mixing them uniformly to obtain finished product of raw reagent liquid. As detecting, taking a raw reagent liquid and adding distilled water to dilute the raw reagent liquid so as to form a liquid reagent, adding rice to the liquid reagent to react, observing the colors of rice and solution, judging the metalolism degree of the rice detected, the metabolism situation and color of the rice corresponds to green and passes through yellow and salmon pink into red region, where the green expresses new rice, yellow expresses old rice and salmon pink expresses deeply aged rice. The reagent of the invention is lower-cost and has simple manufacturing process; the operation is quick, accurate and convenient; the detecting method is simple, able to be used in both qualitative detection and quantitative detection.

The invention discloses a reversible thermochromism material, consisting of the following raw materials in parts by weight: 0.25-8 parts of electron donor compounds, 350-400 parts of electron acceptor compounds and 100 parts of solvent, wherein the electron donor compound is rhodamine B, bromcresol purple or a mixture of the rhodamine B and the bromcresol purple; the electron acceptor compound is boric acid; and the solvent is oxalic acid, tetradecyl alcohol or a mixture of the oxalic acid and the tetradecyl alcohol. A preparation method of the reversible thermochromism material comprises the following steps of: mixing the electron donor compounds, the electron acceptor compounds and the solvent, and grinding the raw materials to have homogeneous colors and phase states, thus obtaining the reversible thermochromism material. The reversible thermochromism material has the characteristics of small environment pollution and low production cost. The adjustment on the color-change temperature of the reversible thermochromism material and the control on the color-recovering time of the reversible thermochromism material can be realized by changing the types of the electron donor compounds and the solvent, the proportion and the reaction temperature of the electron donor compounds, the electron acceptor compounds and the solvents, etc.

The invention discloses a legionnella selective separation medium. Dyes such as bromothymol blue and bromocresol purple are added to the basic formulation of the basic medium of legionnella BCYE alphaand legionnella selective antibiotics of glycin, vancocin, polymyxin B and actinone are also added. The added dyes produce pigment in the growth process of legionnella; the shape and the color of a colony are beneficial to identifying and distinguishing a target bacterium of the legionnella; the concentrations of the selective antibiotics are added and regulated, the inhibiting capability to non-legionnella and microbial class is enhanced and the inhibiting performance for the legionnella is reduced, thereby enhancing the positive rate for the separation culture of the legionnella; the detection cost is reduced and the legionnella selective separation medium is economic and practical and is convenient for popularization and application for clinical and health and epidemic prevention departments.

The invention discloses a pH sensitive freshness detection intelligent label, and a preparation method and application thereof, and belongs to the field of intelligent food packaging, the pH sensitivefreshness detection intelligent label takes bromcresol purple / methyl red as a color developing agent, and polyvinyl alcohol / methyl cellulose as a film forming base material; the polyvinyl alcohol andthe methyl cellulose are wide in source, safe and degradable; bromcresol purple and methyl red are used as common pH indicators and can respond to different pH values. Therefore, the prepared bromcresol purple / methyl red composite pH sensitive intelligent label can be used for accurately monitoring and detecting the freshness of food materials such as livestock and poultry, aquatic products and the like in real time so as to ensure the quality safety of food.

The invention relates to a detecting method and medium of bacillus coagulans. The medium includes 5.0-10.0 parts by weight of tryptone, 2.0-3.0 parts by weight of yeast extract powder / extract, 1.0-5.0parts by weight of glucose, 1.0-2.0 parts by weight of polysorbate-80, 0.1-0.5 part by weight of L-cysteine, 0.04-0.06 part by weight of bromocresol purple, and 15.0-20.0 parts by weight of agar. Themedium allows colonies to grow well, and is low in cost and simple and convenient to prepare. A counting result is accurate and reliable when the medium is utilized for detection of the bacillus coagulans.

The preparation process of cow mammitis streptococcus culture fluid and its use belongs to the field of animal epidemic disease diagnosing, preventing and treating technology. The preparation process includes dissolving peptone 10 g, yeast powder 5 g, sodium chloride 10 g, glucose 5 g and sodium azide 0.5 g in water of 1 L; regulating pH to 7.2 with sodium hydroxide; adding bacteria-free 0.1% concentration crystal violet water solution in 1.3ml and 1% concentration bromocreasol purple water solution in 1.5ml; sterilizing at 121deg.c and preservation at 4 deg. c. The culture fluid may be used in the fast identification of cow mammitis streptococcus and antibiotic sensitivity test.

The invention relates to a method for rapidly detecting coliforms by means of spectrophotometry. By utilizing the characteristic that coliforms can be utilized to ferment lactose at 37 DEG C to produce acid and gas and taking a self-made culture medium as the main role, bromcresol purple serving as an indicator and triphenyltetrazoliumchloride (TTC) solutions are added, after coliforms is fermented and cultured, red precipitates can be produced and culture liquid turns from purple into yellow; the characteristic that the change degree of the color of the fermentation liquid is in positive correlation with the quantity of coliforms contained in the solutions is utilized, the content of coliforms in sample liquid to be detected can be obtained. Compared with an existing national standard method, the method has the advantages that the detection time is greatly shortened, the quantitative value of coliforms in the sample can be detected when coliforms are cultured for 60-400 minutes after sample adding, the trial range is wide, the requirement on short detection time required by various detection fields is met, and the method has good application prospect in the field of rapid detection in the future.

The invention belongs to the technical field of medical examination, and specifically relates to a glycolated serum albumin detection reagent and application thereof. The reagent comprises a reagent R1, a reagent R2 and a reagent R3, wherein the reagent R1 is mainly composed of an MES buffer solution, glycolated amino-acid oxidase, protease, sodium chloride and a preservative; the reagent R2 is mainly composed of the MES buffer solution, N,N-bis(4-sulfobutyl)-3-methylaniline disodium salt, 4-aminoantipyrine, peroxidase, polyol, alkyl polyglucoside and the preservative; and the reagent R3 is mainly composed of an acetate buffer solution, bromocresol purple, alkyl polyglucoside and the preservative. The glycolated serum albumin detection reagent provided by the invention has the advantages of strong anti-interference ability, high precision, good repeatability of determination results, and high detection reagent stability, which facilitates clinical application of the glycolated serum albumin detection reagent.

The invention discloses a rapid diagnosis reagent for mastitis of cattle, sheep and pig, and the components and the weight per cent is: Sodium dodecyl benzene sulfonate 2.5%-thirty%, sodium hydroxide 1%-15.5%, alternate sodium nitride 0.05%-0.5%, bromocresol purple 1%-10%, and water 45%-95%. The invention also discloses the preparation method for the reagent that is making up water solution with sodium hydroxide, alternate sodium nitride and adding sodium dodecylsulfate, bromocresol purple according to the ratio, whisking and mixing to make it fully dissolving. The invention has fast speed, low cost, high accuracy, repetitiveness and good stability. It is easy to operate, and has broad application range.

A method for quickly testing the intestinal flora in beer by test paper includes preparing said test paper and testing. It features that said culture medium contains proportionally water, lactose, peptone, sodium chloride dipotassium hydrogen phosphate, beef extract, agar, bromocresol purple and TTC. Its advantages are high speed, sensitivity and correctness, and low cost.

The invention discloses a microbial time-temperature indicator, which comprises a reaction reagent and a temperature-time indication contrast card. The reaction reagent includes: a Lactobacillus bulgaricus and Streptococcus thermophilus mixed activated bacterial solution, a litmus indicating agent or a bromocresol purple indicating agent, and an 8% skimmed milk medium, and a mixture of the materials is injected into a transparent glass tube to be sealed and stored. The temperature-time indication contrast card sets the corresponding relations of time and color under a fixed temperature. According to the invention, only a reducing substance generated by growth and propagation of Lactobacillus bulgaricus and Streptococcus thermophilus under certain temperature is employed to make the litmus indicating agent or the bromocresol purple indicating agent gradually reduced and faded, then whether food is out of a prescribed transportation or storage temperature in a cold chain circulation process can be judged according to the color by referencing to the temperature-time indication contrast card, so that the food quality in the cold chain circulation process can be reflected visually.

The invention relates to a solid medium for detoxification of sweet potato stem tips and a preparation method thereof, which is composed of the following raw materials in parts by weight: 100-200 parts of agar; 43-58 parts of mother liquor I; 0.65-1.19 parts of mother liquor II; 3 parts of glycine ‑6 parts; thiamine hydrochloride 0.3‑0.5 parts; inosose 50‑65 parts; sucrose 80‑100 parts; bromocresol purple 1‑3 parts; indole‑3‑acetic acid 0.2‑0.4 parts; ; The preparation method: first prepare the base medium; then make the complete medium; add agar to stir and mix, and prepare the solid medium after high-pressure steam sterilization. The present invention facilitates the adjustment of the pH value of the solid medium by adding bromocresol violet; by adding sucrose, the germination rate of the shoot tip is greatly improved; by adding indole-3-acetic acid, the ability of the shoot tip to form callus is improved , the seedlings grow quickly, thus greatly shortening the cultivation period and saving the production cost.

The invention discloses a fecal coliform chromogenic culture medium and application thereof, belonging to the technical field of medical inspection. The fecal coliform chromogenic culture medium is composed of 10-20 g / L peptone, 2-4 g / L animal tissue digested substance, 5-15 g / L lactose, 0.5-1.5 g / L bile salt, 0.2-0.4 g / L lauryl sodium sulfate, 0.03-0.06 g / L bromocresol purple, 1-3g of lecithin, 10-15 g / L Tween 80 and the balance of deionized water. The pH value is 7.4+ / -0.2. The culture medium is used for detecting fecal coliform in cosmetics, and has the advantages of high detection sensitivity and high specificity; the culture medium can be used for identifying the strain according to the color, so the detection period is short, and the culture medium is suitable for treating massive samples; and the culture medium can easily implement industrialized production, can be used for carrying out comprehensive, systematic and accurate detection and preliminary identification on fecal coliform in cosmetics, and provides a new way for cosmetic detection.

The invention discloses a nutrient solution for detecting bacterial vaginosis. Each 100ml of nutrient solution contains the following raw materials: 0.1-1g of beef heart powder, 0.5-5g of tryptone, 0.1-2g of glucose, 0.1-1g of sodium chloride, 0.1-1g of bromocresol purple sodium, 5-20ml of horse serum, 0.0001-0.001g of neomycin sulfate and 0.0001-0.001g of nystatin. The invention further discloses a kit for detecting bacterial vaginosis and a detection method. The detection kit is stable in quality; the detection method is simple in operation and accurate in result. The kit has the functions of target cultivation and simultaneous detection of the sensitivity of a drug, the detection method is completed in one step within 6-24h, the success rate is high, the drug sensitivity result is reliable, clinical accurate diagnosis of the bacterial vaginosis and the drug sensitivity test can be met, a reference foundation is provided for a clinician in timely accurate diagnosis and reasonable use of the drug, and clinical abuse of antibiotics is avoided.

The invention discloses a kit for fungal rapid culture, identification and drug sensitivity, and belongs to the technical field of drug sensitivity detection. The kit includes a culture liquid, a diluent, mineral oil and a detection plate; the culture liquid contains tryptone, NaCl, Na2HPO4, dicloran, ammonium sulfate, a standard fetal bovine serum, ferrous sulfate, potassium chloride, an egg yolk liquid, sucrose, glucose, vancomycin, chloramphenicol, and bromocresol purple; the detection plate is provided with a fungal identification hole and an antibiotic drug sensitivity detection hole. According to the kit, sucrose and glucose efficient growth factors and other effective components are added in the used culture liquid, so that the rapid growth of fungi can be guaranteed, culture of the fungi is completed in 24 h, a result can be obtained in 24 h, the period of clinical detection is greatly shortened, and early diagnosis and treatment of patients are facilitated.

The invention belongs to the technical field of glycated protein detection, and particularly relates to a method for detecting glycated serum albumin by using a boric acid affinity principle. The method is characterized by comprising the following steps: taking a certain volume of bromcresol purple solution in a cuvette; adding a serum sample to be detected into the bromcresol purple solution; obtaining the total protein concentration CT by measuring the absorbance of 585 nm; adding a phenylboronic acid adsorbent into the cuvette to react with the BCP-GA; waiting until the adsorbent is completely deposited at the bottom of the cuvette; and measuring the absorbance of 585 nm to obtain the concentration Cng of the non-glycated serum albumin, and calculating the proportion of the glycated serum albumin through a formula (CT-Cng) / CT * 100%. According to the method, the bromcresol purple is used for testing the total albumin content under the alkaline condition, the absorbance is reduced after the albumin and the bromcresol purple are combined, the albumin content and the absorbance decline value are in a linear relation, all tests are completed through the absorbance spectrophotometry, reagents are stable, detection is rapid, simple and convenient, and the method is completely compatible with general equipment in hospitals.

The invention discloses a freshness indicating electrostatic spinning fiber membrane and a preparation method and application thereof. The preparation method of the electrostatic spinning fiber membrane comprises the following steps of mixing polyvinyl alcohol and water, heating for 30-90 min at the temperature of 80 DEG C under a stirring condition so that polyvinyl alcohol can be dissolved in water, reducing the temperature to room temperature of 20-25 DEG C, adding a bromocresol purple ethanol solution, uniformly stirring, adjusting the pH value to 5, uniformly mixing, defoaming to obtain amixture, and spinning the mixture obtained in the step 1) into a membrane by using an electrostatic spinning device to obtain the electrostatic spinning fiber membrane. The preparation method disclosed by the invention is simple to operate and low in cost, and the obtained electrostatic spinning fiber membrane has the function of indicating the freshness of meat products; the electrostatic spinning fiber membrane is yellow before use, and is gradually changed into blue along with the decay of the meat product after being applied to the preservation of the meat product.

The invention relates to a long-chain alkyltrimethyl ammonium sensitizing mixed reagent paper for measuring ammonia in air and preparation method thereof, belongs to the field of rapid environmental detection and industrial hygiene. The test paper is loaded with bromocresol purple sodium salt, bromothymol blue sodium salt, and surfactant long-chain alkyl trimethyl ammonium on the base paper according to the mass ratio, bromocresol purple sodium salt: bromo thymol blue sodium salt: surfactant long-chain alkyl trimethyl ammonium = (4~8): (6~10): (0.02~0.2). The preparation method comprises the following steps of: preparing the soaking solution according to the load reagent; soaking the base paper; drying and encapsulating. The test paper is made to change from the yellow to blue-violet basedon the reaction between ammonia gas in combination with bromocresol purple sodium salt and bromothymol blue sodium salt. The color change is obvious, the color of the test paper reaction is clear, itis easy to observe and measure, and the precision is high. The long-chain alkyltrimethyl ammonium makes the bromocresol purple sodium salt and the bromothymol blue sodium salt disperse better, and makes its uniformity on the filter paper better, thus the length of the test paper discoloration band is increased, and the sensitivity is increased.

A reagent composition is added to test water, the reagent composition being prepared by dissolving methyl red having an acid dissociation constant (pKa) of 5.1, phenol red having a pKa of 7.7 (higherthan that of the methyl red), and bromocresol purple having a pKa of 6.3 (between those of methyl red and phenol red) at respective prescribed proportions in ethylene glycol or another such diol. Thelight absorbance of the test water to which the reagent composition was added is measured with respect to three wavelengths, i.e., a wavelength selected from the range of 410-430 nm, a wavelength selected from the range of 515-535 nm, and a wavelength selected from the range of 580-600 nm, and the pH of the test water is assessed on the basis of the light absorbance. This makes it possible to measure the pH of the test water within a range of 4-9.

The invention discloses a rapid diagnosis reagent for mastitis of cattle, sheep and pig, and the components and the weight per cent is: Sodium dodecyl benzene sulfonate 2.5%-thirty%, sodium hydroxide 1%-15.5%, alternate sodium nitride 0.05%-0.5%, bromocresol purple 1%-10%, and water 45%-95%. The invention also discloses the preparation method for the reagent that is making up water solution with sodium hydroxide, alternate sodium nitride and adding sodium dodecylsulfate, bromocresol purple according to the ratio, whisking and mixing to make it fully dissolving. The invention has fast speed, low cost, high accuracy, repetitiveness and good stability. It is easy to operate, and has broad application range.

The invention discloses a method for highly-efficient detection of gas-producing bacteria in vinegar by combining bromcresol purple and a Durham tube. According to the invention, a culture medium suitable for the growth and reproduction of the gas-producing bacteria in the vinegar is prepared; a bromocresol purple indicator is added into the culture medium; in addition, a glucose solution with a content approximate to 13.5% is prepared; the culture medium and the glucose solution are separately sterilized and then combined for culturing of the gas-producing bacteria; after the pH value of a to-be-detected vinegar liquid is adjusted to 6.5 to 7.0, the to-be-detected vinegar liquid is inoculated onto the culture medium; observation is performed after culturing at 33+ / -1 DEG C for 48+ / -2 h,;if a culture solution is yellow, or air bubbles are generated in the Durham tube, or the culture solution is turbid, the gas-producing bacteria are proved to exist in a detected original sample; and if the culture solution is purple, and no air bubbles are generated in the Durham tube, the gas-producing bacteria are proved to not exist in the detected original sample. The method provided by the invention can accurately distinguish the content level of the gas-producing bacteria in the detected vinegar liquid, can accurately judge whether a vinegar liquid with low content of the gas-producing bacteria contains the gas-producing bacteria or not, and is accurate in detection results.

The invention discloses a water-based precoating detection agent for digital printing. The water-based precoating detection agent comprises the following components in percentage by mass of 1-3% of bromcresol purple, 10-20% of deionized water, 76.7-88.7% of ethanol with the purity of 95 percent, and 0.3% of NaOH. The invention further provides a corresponding detection pen and a detection method.The water-based precoating detection agent is advantaged in that the prepared detection agent reacts with a transparent pre-coating layer coated on a printing material, coating uniformity and bondingfirmness of the transparent pre-coating layer coated on the printing material are intuitively reflected by detecting the color development change, the color development uniformity and the coherence ofthe agent, compared with the prior art that the coating effect of the transparent pre-coating layer on the printing material is fed back completely through production experience and the actual printing effect, a coating quality problem can be effectively solved in time, production efficiency is obviously improved, loss of the printing material is reduced, and production cost is saved.

The invention discloses an application of Citrobacter sp. JPG1 in removal of bromcresol purple and acid golden yellow G, and belongs to the technical field of microorganisms. The Citrobacter JPG1 is preserved in China General Microbiological Culture Collection Center on December 22, 2016 with the preservation number of CGMCC NO.13480, and the strain has good ability to remove the bromcresol purpleunder an aerobic condition and the acid golden yellow G under an anaerobic condition. When the respectively concentration of the bromcresol purple and the acid golden yellow G is 200 mg / L and the initial inoculum size of the strain is 10%, the removal rate of the p-bromocresol purple at 36 h reaches 73.64%, and the removal rate of the acid golden yellow G at 36 h reaches 87.51%. The Citrobactersp. JPG1 is an efficient dye removal bacterium, can be directly applied to biological treatment of dye wastewater containing bromcresol purple and acid golden yellow G, and has good application prospects in anaerobic and aerobic treatments.

The invention relates to the technical field of food detection, in particular to a method for detecting antibiotics in food, which comprises the following steps: food pretreatment, culture medium preparation and antibiotic detection, and detection of antibodies in food through a series of steps of crushing and pulping, oscillation and dispersion, buffer extraction, standing and layering, extraction and filtration, culture medium prefabrication, spore cultivation and observation and detection. According to the method, whether the content of antibiotics in food reaches the standard or not can be detected according to the color change through the arranged pH indicator (bromcresol purple), the detection result is more visual, and compared with data measurement and calculation, the steps of detection, recording, measurement, comparison and the like are needed, and the detection efficiency is greatly improved through a retrograde detection method; the detection method is effectively simplified.

The invention discloses a dual-visualization ink and preparing method thereof, which includes 79-83 percent offset resin, 10-8 percent of A visualization reagent, 10-8 percent B visualization reagent and 1 percent of drier. The offset resin is offset phenolic resin and alkyd resin, the A visualization reagent is zinc salicylate and the B visualization reagent is bromocresol purple or bromocresol green, and the driers is red drying oil. The preparing method for the dual-visualization ink includes the following steps: first, accurately weighting, stirring and mixing the offset resin, the A visualization reagent, the B visualization reagent and the drier; second, grinding the mixture by a three-roll mill; third, when grinding fineness is less than or equals to 15mum, obtaining the ink. The invention has the beneficial effects that the antifake effect is enhanced and the visualization effect is stable and reliable due to use of the ink made of two kinds of visualization reagents. The dual-visualization ink is convenient for preparation and is suitable for batch printing.

The preparation process of cow mammitis streptococcus culture fluid and its use belongs to the field of animal epidemic disease diagnosing, preventing and treating technology. The preparation process includes dissolving peptone 10 g, yeast powder 5 g, sodium chloride 10 g, glucose 5 g and sodium azide 0.5 g in water of 1 L; regulating pH to 7.2 with sodium hydroxide; adding bacteria-free 0.1% concentration crystal violet water solution in 1.3ml and 1% concentration bromocreasol purple water solution in 1.5ml; sterilizing at 121deg.c and preservation at 4 deg. c. The culture fluid may be used in the fast identification of cow mammitis streptococcus and antibiotic sensitivity test.

The invention discloses bis(4-pyridine)cresol purple sultones as well as a preparation method and an application method thereof. The structural formula is shown in the description. The structure of the compound is to replace the bromine with 4-pyridine group on the basis of a bromcresol purple nuclear parent, so that a detection function of bromcresol purple sultones can be kept. The 4-pyridine group is used for substituting the bromine atoms to design and synthesize bis(4-pyridine)cresol purple sultones, so that bis(4-pyridine)cresol purple sultones has the advantage in detecting the contentof albumin in serum as follows: firstly, the poor water solubility of the bromcresol purple compound is changed, the water solubility of the compound is improved, and the detection sensitivity is improved; and secondly, bis(4-pyridine)cresol purple sultones contains no bromine atoms, so that the non-specific hydrophobic combination between the bromine atoms and various proteins can be reduced, andbis(4-pyridine) cresol purple sultones has better specificity for combining the albumin than that of the dye bromcresol purple compound, and can be used for detecting the content of the albumin.

The invention relates to stability augmentation test paper for determining ammonia in air by a drying method and preparation and detection methods thereof, and belongs to the fields of environmental monitoring technology and industrial hygiene. The stability augmentation test paper for determining ammonia in the air by the drying method is the test paper loaded with a bromocresol purple-bromothymol blue mixed indicator and a cationic surfactant on base paper, which has a mass ratio of bromocresol purple: bromothymol blue: cationic surfactant = (0.1-0.55): (0.025-0.2): (X-X'). An X-X' value varies depending on the surfactant. The preparation method comprises the following steps of: preparing a soaking liquid according to a reagent ratio; soaking the base paper; and drying and carrying out plastic packaging. The detection method comprises the following steps of: detecting the reaction of ammonia in the air and a mixed reagent of the test paper; turning from yellow to bluish violet; and obtaining the concentration of the ammonia in the air of a detection area according to the relationship between the length of a color changing belt of the test paper and the ammonia concentration. Thedetection method can be used to measure the ammonia concentration in the air of a workshop or a production site or the emergency monitoring of the ammonia leakage.

The invention provides a carbapenemase-producing strain rapid detection method and kit, and an application of the kit. The rapid detection method includes the steps: carrying out common incubation ofimipenem with a to-be-tested bacterial lawn to prepare an MH agar culture medium containing bromcresol purple and acid-producing bacteria, pasting imipenem paper after incubation with the to-be-testedbacterial lawn on the MH agar culture medium containing the bromocresol purple and the acid-producing bacteria, incubating for 3.5-4.5 h, and determining whether the to-be-tested strain is a carbapenemase-producing strain directly through a fact whether bromocresol purple is discolored. The detection method has the advantages of simple operation, fast analysis, wide application range, high accuracy and specificity, and easy interpretation of the results. At the same time, the invention also provides the detection kit established based on the rapid detection method; the kit has important significance for early discovery of the carbapenemase-producing strain and adopting of effective measures, nosocomial infection control and clinical epidemiological detection, and has good application prospects.