45 results about "Bromocresol purple" patented technology

The invention relates to a rice metabolism detecting reagent and method, concretely used in the condition of detecting rice metabolism. It mainly takes and dissolves nitrazine yellow/chlorophenol red, p-nitrophenol, bromocresol green/bromothymol blue/resorcinol blue, bromocresol purple, and hematoxylin/cresol red/bromophenol red/methyl red into alcohol, and mixing them uniformly to obtain finished product of raw reagent liquid. As detecting, taking a raw reagent liquid and adding distilled water to dilute the raw reagent liquid so as to form a liquid reagent, adding rice to the liquid reagent to react, observing the colors of rice and solution, judging the metalolism degree of the rice detected, the metabolism situation and color of the rice corresponds to green and passes through yellow and salmon pink into red region, where the green expresses new rice, yellow expresses old rice and salmon pink expresses deeply aged rice. The reagent of the invention is lower-cost and has simple manufacturing process; the operation is quick, accurate and convenient; the detecting method is simple, able to be used in both qualitative detection and quantitative detection.

The invention discloses a legionnella selective separation medium. Dyes such as bromothymol blue and bromocresol purple are added to the basic formulation of the basic medium of legionnella BCYE alphaand legionnella selective antibiotics of glycin, vancocin, polymyxin B and actinone are also added. The added dyes produce pigment in the growth process of legionnella; the shape and the color of a colony are beneficial to identifying and distinguishing a target bacterium of the legionnella; the concentrations of the selective antibiotics are added and regulated, the inhibiting capability to non-legionnella and microbial class is enhanced and the inhibiting performance for the legionnella is reduced, thereby enhancing the positive rate for the separation culture of the legionnella; the detection cost is reduced and the legionnella selective separation medium is economic and practical and is convenient for popularization and application for clinical and health and epidemic prevention departments.

The preparation process of cow mammitis streptococcus culture fluid and its use belongs to the field of animal epidemic disease diagnosing, preventing and treating technology. The preparation process includes dissolving peptone 10 g, yeast powder 5 g, sodium chloride 10 g, glucose 5 g and sodium azide 0.5 g in water of 1 L; regulating pH to 7.2 with sodium hydroxide; adding bacteria-free 0.1% concentration crystal violet water solution in 1.3ml and 1% concentration bromocreasol purple water solution in 1.5ml; sterilizing at 121deg.c and preservation at 4 deg. c. The culture fluid may be used in the fast identification of cow mammitis streptococcus and antibiotic sensitivity test.

The invention discloses a rapid diagnosis reagent for mastitis of cattle, sheep and pig, and the components and the weight per cent is: Sodium dodecyl benzene sulfonate 2.5%-thirty%, sodium hydroxide 1%-15.5%, alternate sodium nitride 0.05%-0.5%, bromocresol purple 1%-10%, and water 45%-95%. The invention also discloses the preparation method for the reagent that is making up water solution with sodium hydroxide, alternate sodium nitride and adding sodium dodecylsulfate, bromocresol purple according to the ratio, whisking and mixing to make it fully dissolving. The invention has fast speed, low cost, high accuracy, repetitiveness and good stability. It is easy to operate, and has broad application range.

A method for quickly testing the intestinal flora in beer by test paper includes preparing said test paper and testing. It features that said culture medium contains proportionally water, lactose, peptone, sodium chloride dipotassium hydrogen phosphate, beef extract, agar, bromocresol purple and TTC. Its advantages are high speed, sensitivity and correctness, and low cost.

The invention relates to a solid medium for detoxification of sweet potato stem tips and a preparation method thereof, which is composed of the following raw materials in parts by weight: 100-200 parts of agar; 43-58 parts of mother liquor I; 0.65-1.19 parts of mother liquor II; 3 parts of glycine ‑6 parts; thiamine hydrochloride 0.3‑0.5 parts; inosose 50‑65 parts; sucrose 80‑100 parts; bromocresol purple 1‑3 parts; indole‑3‑acetic acid 0.2‑0.4 parts; ; The preparation method: first prepare the base medium; then make the complete medium; add agar to stir and mix, and prepare the solid medium after high-pressure steam sterilization. The present invention facilitates the adjustment of the pH value of the solid medium by adding bromocresol violet; by adding sucrose, the germination rate of the shoot tip is greatly improved; by adding indole-3-acetic acid, the ability of the shoot tip to form callus is improved , the seedlings grow quickly, thus greatly shortening the cultivation period and saving the production cost.

The invention discloses a fecal coliform chromogenic culture medium and application thereof, belonging to the technical field of medical inspection. The fecal coliform chromogenic culture medium is composed of 10-20 g/L peptone, 2-4 g/L animal tissue digested substance, 5-15 g/L lactose, 0.5-1.5 g/L bile salt, 0.2-0.4 g/L lauryl sodium sulfate, 0.03-0.06 g/L bromocresol purple, 1-3g of lecithin, 10-15 g/L Tween 80 and the balance of deionized water. The pH value is 7.4+/-0.2. The culture medium is used for detecting fecal coliform in cosmetics, and has the advantages of high detection sensitivity and high specificity; the culture medium can be used for identifying the strain according to the color, so the detection period is short, and the culture medium is suitable for treating massive samples; and the culture medium can easily implement industrialized production, can be used for carrying out comprehensive, systematic and accurate detection and preliminary identification on fecal coliform in cosmetics, and provides a new way for cosmetic detection.

The invention discloses a nutrient solution for detecting bacterial vaginosis. Each 100ml of nutrient solution contains the following raw materials: 0.1-1g of beef heart powder, 0.5-5g of tryptone, 0.1-2g of glucose, 0.1-1g of sodium chloride, 0.1-1g of bromocresol purple sodium, 5-20ml of horse serum, 0.0001-0.001g of neomycin sulfate and 0.0001-0.001g of nystatin. The invention further discloses a kit for detecting bacterial vaginosis and a detection method. The detection kit is stable in quality; the detection method is simple in operation and accurate in result. The kit has the functions of target cultivation and simultaneous detection of the sensitivity of a drug, the detection method is completed in one step within 6-24h, the success rate is high, the drug sensitivity result is reliable, clinical accurate diagnosis of the bacterial vaginosis and the drug sensitivity test can be met, a reference foundation is provided for a clinician in timely accurate diagnosis and reasonable use of the drug, and clinical abuse of antibiotics is avoided.

The invention discloses a kit for fungal rapid culture, identification and drug sensitivity, and belongs to the technical field of drug sensitivity detection. The kit includes a culture liquid, a diluent, mineral oil and a detection plate; the culture liquid contains tryptone, NaCl, Na2HPO4, dicloran, ammonium sulfate, a standard fetal bovine serum, ferrous sulfate, potassium chloride, an egg yolk liquid, sucrose, glucose, vancomycin, chloramphenicol, and bromocresol purple; the detection plate is provided with a fungal identification hole and an antibiotic drug sensitivity detection hole. According to the kit, sucrose and glucose efficient growth factors and other effective components are added in the used culture liquid, so that the rapid growth of fungi can be guaranteed, culture of the fungi is completed in 24 h, a result can be obtained in 24 h, the period of clinical detection is greatly shortened, and early diagnosis and treatment of patients are facilitated.

The invention discloses a freshness indicating electrostatic spinning fiber membrane and a preparation method and application thereof. The preparation method of the electrostatic spinning fiber membrane comprises the following steps of mixing polyvinyl alcohol and water, heating for 30-90 min at the temperature of 80 DEG C under a stirring condition so that polyvinyl alcohol can be dissolved in water, reducing the temperature to room temperature of 20-25 DEG C, adding a bromocresol purple ethanol solution, uniformly stirring, adjusting the pH value to 5, uniformly mixing, defoaming to obtain amixture, and spinning the mixture obtained in the step 1) into a membrane by using an electrostatic spinning device to obtain the electrostatic spinning fiber membrane. The preparation method disclosed by the invention is simple to operate and low in cost, and the obtained electrostatic spinning fiber membrane has the function of indicating the freshness of meat products; the electrostatic spinning fiber membrane is yellow before use, and is gradually changed into blue along with the decay of the meat product after being applied to the preservation of the meat product.

The invention relates to a long-chain alkyltrimethyl ammonium sensitizing mixed reagent paper for measuring ammonia in air and preparation method thereof, belongs to the field of rapid environmental detection and industrial hygiene. The test paper is loaded with bromocresol purple sodium salt, bromothymol blue sodium salt, and surfactant long-chain alkyl trimethyl ammonium on the base paper according to the mass ratio, bromocresol purple sodium salt: bromo thymol blue sodium salt: surfactant long-chain alkyl trimethyl ammonium = (4~8): (6~10): (0.02~0.2). The preparation method comprises the following steps of: preparing the soaking solution according to the load reagent; soaking the base paper; drying and encapsulating. The test paper is made to change from the yellow to blue-violet basedon the reaction between ammonia gas in combination with bromocresol purple sodium salt and bromothymol blue sodium salt. The color change is obvious, the color of the test paper reaction is clear, itis easy to observe and measure, and the precision is high. The long-chain alkyltrimethyl ammonium makes the bromocresol purple sodium salt and the bromothymol blue sodium salt disperse better, and makes its uniformity on the filter paper better, thus the length of the test paper discoloration band is increased, and the sensitivity is increased.

The invention discloses a method for highly-efficient detection of gas-producing bacteria in vinegar by combining bromcresol purple and a Durham tube. According to the invention, a culture medium suitable for the growth and reproduction of the gas-producing bacteria in the vinegar is prepared; a bromocresol purple indicator is added into the culture medium; in addition, a glucose solution with a content approximate to 13.5% is prepared; the culture medium and the glucose solution are separately sterilized and then combined for culturing of the gas-producing bacteria; after the pH value of a to-be-detected vinegar liquid is adjusted to 6.5 to 7.0, the to-be-detected vinegar liquid is inoculated onto the culture medium; observation is performed after culturing at 33+/-1 DEG C for 48+/-2 h,;if a culture solution is yellow, or air bubbles are generated in the Durham tube, or the culture solution is turbid, the gas-producing bacteria are proved to exist in a detected original sample; and if the culture solution is purple, and no air bubbles are generated in the Durham tube, the gas-producing bacteria are proved to not exist in the detected original sample. The method provided by the invention can accurately distinguish the content level of the gas-producing bacteria in the detected vinegar liquid, can accurately judge whether a vinegar liquid with low content of the gas-producing bacteria contains the gas-producing bacteria or not, and is accurate in detection results.

The invention discloses a water-based precoating detection agent for digital printing. The water-based precoating detection agent comprises the following components in percentage by mass of 1-3% of bromcresol purple, 10-20% of deionized water, 76.7-88.7% of ethanol with the purity of 95 percent, and 0.3% of NaOH. The invention further provides a corresponding detection pen and a detection method.The water-based precoating detection agent is advantaged in that the prepared detection agent reacts with a transparent pre-coating layer coated on a printing material, coating uniformity and bondingfirmness of the transparent pre-coating layer coated on the printing material are intuitively reflected by detecting the color development change, the color development uniformity and the coherence ofthe agent, compared with the prior art that the coating effect of the transparent pre-coating layer on the printing material is fed back completely through production experience and the actual printing effect, a coating quality problem can be effectively solved in time, production efficiency is obviously improved, loss of the printing material is reduced, and production cost is saved.

The invention discloses an application of Citrobacter sp. JPG1 in removal of bromcresol purple and acid golden yellow G, and belongs to the technical field of microorganisms. The Citrobacter JPG1 is preserved in China General Microbiological Culture Collection Center on December 22, 2016 with the preservation number of CGMCC NO.13480, and the strain has good ability to remove the bromcresol purpleunder an aerobic condition and the acid golden yellow G under an anaerobic condition. When the respectively concentration of the bromcresol purple and the acid golden yellow G is 200 mg/L and the initial inoculum size of the strain is 10%, the removal rate of the p-bromocresol purple at 36 h reaches 73.64%, and the removal rate of the acid golden yellow G at 36 h reaches 87.51%. The Citrobactersp. JPG1 is an efficient dye removal bacterium, can be directly applied to biological treatment of dye wastewater containing bromcresol purple and acid golden yellow G, and has good application prospects in anaerobic and aerobic treatments.

The invention relates to the technical field of food detection, in particular to a method for detecting antibiotics in food, which comprises the following steps: food pretreatment, culture medium preparation and antibiotic detection, and detection of antibodies in food through a series of steps of crushing and pulping, oscillation and dispersion, buffer extraction, standing and layering, extraction and filtration, culture medium prefabrication, spore cultivation and observation and detection. According to the method, whether the content of antibiotics in food reaches the standard or not can be detected according to the color change through the arranged pH indicator (bromcresol purple), the detection result is more visual, and compared with data measurement and calculation, the steps of detection, recording, measurement, comparison and the like are needed, and the detection efficiency is greatly improved through a retrograde detection method; the detection method is effectively simplified.

The invention discloses a dual-visualization ink and preparing method thereof, which includes 79-83 percent offset resin, 10-8 percent of A visualization reagent, 10-8 percent B visualization reagent and 1 percent of drier. The offset resin is offset phenolic resin and alkyd resin, the A visualization reagent is zinc salicylate and the B visualization reagent is bromocresol purple or bromocresol green, and the driers is red drying oil. The preparing method for the dual-visualization ink includes the following steps: first, accurately weighting, stirring and mixing the offset resin, the A visualization reagent, the B visualization reagent and the drier; second, grinding the mixture by a three-roll mill; third, when grinding fineness is less than or equals to 15mum, obtaining the ink. The invention has the beneficial effects that the antifake effect is enhanced and the visualization effect is stable and reliable due to use of the ink made of two kinds of visualization reagents. The dual-visualization ink is convenient for preparation and is suitable for batch printing.

The invention discloses bis(4-pyridine)cresol purple sultones as well as a preparation method and an application method thereof. The structural formula is shown in the description. The structure of the compound is to replace the bromine with 4-pyridine group on the basis of a bromcresol purple nuclear parent, so that a detection function of bromcresol purple sultones can be kept. The 4-pyridine group is used for substituting the bromine atoms to design and synthesize bis(4-pyridine)cresol purple sultones, so that bis(4-pyridine)cresol purple sultones has the advantage in detecting the contentof albumin in serum as follows: firstly, the poor water solubility of the bromcresol purple compound is changed, the water solubility of the compound is improved, and the detection sensitivity is improved; and secondly, bis(4-pyridine)cresol purple sultones contains no bromine atoms, so that the non-specific hydrophobic combination between the bromine atoms and various proteins can be reduced, andbis(4-pyridine) cresol purple sultones has better specificity for combining the albumin than that of the dye bromcresol purple compound, and can be used for detecting the content of the albumin.