115 results about "Bromocresol green" patented technology

The invention discloses an albumin detection reagent which comprises a diluent and a reaction reagent, wherein the diluent is composed of trihydroxymethyl amino buffer, surfactant, preservative, anti-bilirubin interference agent and vitamin C oxidase; and the reaction reagent is composed of buffer, surfactant, bromocresol green and freeze-drying protective agent. The detection reagent disclosed by the invention has favorable sensitivity, accuracy, precision and linearity, and can completely satisfy the clinical examination requirements.

The invention discloses a method for identifying human blood albumin products, comprising the following steps: (1) a citric acid buffer solution is prepared: 0.2mol/L of citric acid and 0.2mol/L of sodium citrate are taken and mixed according to the volume proportion of 12.3: 7.7, and each liter of buffer solution is added with 100mg of sodium azide, then the pH is adjusted to 4.2 by using 0.2mol/L of citric acid and 0.2mol/L of sodium citrate; (2) a BCG reagent is prepared: 105mg of bromocresol green is weighed and firstly dissolved in 2.5ml of 0.1mol/L NaOH (water-jacket heating for dissolution enhancement), then the mixture is added with 1000ml of 0.2mol/L citric acid buffer solution with the pH of 4.2, and 1 to 2ml of Tween minus 80 is added; (3) 2ml of BCG reagent is taken to be added with one drop of human serum albumin product which is ready to be detected, and an even shaking is carried out. If the color of the solution turns green from yellow, the product is an authentic product; otherwise, the product is a schlock. The identification method of the invention has low analysis cost, no need of special equipment, stronger specificity and high correct rate of the inspection results; the invention is applicable to the rapid inspection method of human blood albumin at the scene, and meets the needs of the basic pharmaceutical supervision and inspection.

A new optical sensing method for detection of analyte vapors down to ppb levels is described. The sensor is based on the use of a visible indicator, such as Bromocresol green, adsorbed onto a high surface area substrate, such as a silica sphere matrix. When the analyte gas is adsorb onto the matrix, the indicator undergoes a color change. The color change in turn is detected with a suitable spectrometer. Sensor performance is demonstrated for an exemplary amine sensor for the aliphatic amines tert-butylamine, diethylamine and triethylamine and also for pyridine and aniline. The microsphere sensor is more sensitive than other prior art optical amine sensor designs. The sensor response varies with temperature, with lower sensitivity and faster response at higher temperatures allowing for adjustment to prioritize sensitivity or speed. The sensor response is also highly reproducible and fully reversible.

The invention relates to a piece of highly sensitive pH test paper and a preparation method thereof, wherein a developing strip is in the form of a piece of pH body paper, which is dipped in a reagent solution and then dried by hot air at constant temperature; the reagent solution contains an indicator; the formula of the indicator contains bromothymol blue, thymol blue, metanil yellow, m-methyl red, bromocresol green, nitrazine yellow, cresol red, phenolphthalein, para-nitrophenylazosalicylate sodium, titan yellow, fluorescent pigment orange red, 5% 4-nitrobenzenediazo-1-naphthol-3.6-sodium disulfonate solution and 30% dissolved ethanol aqueous solution; and the formula of the indicator further contains an aqueous colour fixing agent, a cotton wooden fibre mildew preventive, a special paper mildew preventive and a buffer neutralizer formed by modulating sodium hydroxide and phosphoric acid. The preparation method comprises the following steps of: adjusting the reagent solution till a pH value is up to 6-7.5, uniformly wetting the pH body paper, and drying through hot air at constant temperature. According to the invention, the reagent solution can be adjusted, so that the pH value is up to 6-7.5; steady and precise high sensitivity of the pH1-14 test paper is effectively increased; and the test paper is convenient and simple to use.

Water-based chemical indicator inks for ethylene oxide sterilization processes and methods for its use. The chemical indicator ink contains at least one pH indicator dye selected from the group consisting of Bromocresol green, Bromophenol blue, Methyl red, Ethyl orange, and combinations thereof. The pH indicator dye undergoes an irreversible color change when exposed to ethylene oxide vapor in the presence of low-temperature steam, but when exposed to other sterilization processes either does not undergo a color change or undergoes a color change that is different than is obtained when exposed to ethylene oxide.

The invention provides a humidity indicator which is really effective in environmental protection and comprises a carrier and solution used for soaking the carrier; wherein, the solution takes 30-80% of ethanol as a solvent and contains 0.00862-0.00992% of acid-base indicator and 0.8-13.8% of hygroscopic salt. The acid-base indicator is one of bromocresol green, bromothymol blue, thymol blue, thymolphthalein, bromophenol blue, methyl red, methyl orange, methyl yellow , neutral red and phenol red or the mixture thereof. The hygroscopic salt is alkali halide or alkali-earth metal halide. The humidity indicator of the invention has wide indicating range (capable of indicating 5-90% of ambient humidity) and high sensitivity. Diverse humidity indicators different in discoloration can be manufactured by adjusting the components. The humidity indicator of the invention has evident discoloration effect, simple use, reutilization, good suitability and extensive application.

The invention relates to a rice metabolism detecting reagent and method, concretely used in the condition of detecting rice metabolism. It mainly takes and dissolves nitrazine yellow/chlorophenol red, p-nitrophenol, bromocresol green/bromothymol blue/resorcinol blue, bromocresol purple, and hematoxylin/cresol red/bromophenol red/methyl red into alcohol, and mixing them uniformly to obtain finished product of raw reagent liquid. As detecting, taking a raw reagent liquid and adding distilled water to dilute the raw reagent liquid so as to form a liquid reagent, adding rice to the liquid reagent to react, observing the colors of rice and solution, judging the metalolism degree of the rice detected, the metabolism situation and color of the rice corresponds to green and passes through yellow and salmon pink into red region, where the green expresses new rice, yellow expresses old rice and salmon pink expresses deeply aged rice. The reagent of the invention is lower-cost and has simple manufacturing process; the operation is quick, accurate and convenient; the detecting method is simple, able to be used in both qualitative detection and quantitative detection.

The invention provides a stable kit for detecting glycation albumin. The stable kit consists of a reagent R1, a reagent R2 and a reagent R3, wherein the reagent R1 comprises the following components: a buffering solution, glycation amino acid oxidase, albumin protease, peroxidase, catalase, 4-aminoantipyrene, a stabilizing agent and a preservative; the reagent R2 comprises the following components: a buffering solution, albumin protease, peroxidase, chromogen, a stabilizing agent and a preservative; the reagent R3 comprises the following components: a buffering solution, bromocresol green, a surface active agent and a preservative. The stable kit provided by the invention has the advantages that the influence on the detection result caused by endogenous glycation amino acid is removed by adding glycation amino acid oxidase, so that the result is accurate; the stable kit adopts an enzyme method to detect the content of the glycation albumin, and is a simple, fast, sensitive and accurate detection method.

The invention discloses a method for determining total alkaloid content in black nightshade with an ultraviolet spectrophotometer by adopting an acid dye colorimetric method. The method is characterized in that by adopting black nightshade alkali as a reference substance, a reference substance solution is prepared by using chloroform; ultrasonic extraction of total alkaloid in black nightshade is carried out by adopting an acid-alcohol mixed solvent to prepare a test solution; and chromogenic conditions of the acid dye colorimetric method are as follows: pH is equal to 3.6, 3.5 mL of a buffer solution and 2.0 mL of a bromocresol green solution are used, extraction is carried out three times with 10 mL of chloroform, and standing is kept for 30 min. The detection wavelength is 243 nm, and content determination is carried out according to the absorbance. The total alkaloid of the black nightshade is in a linear relationship within a range of 5-30 mu g/mL, the average value of recovery rate is 99.7%, and RSD is equal to 1.4% (n is equal to 6). The method for determining the total alkaloid content in black nightshade disclosed by the invention is simple and convenient to operate, low in cost, high in recovery rate and good in reproducibility, and can be used for determining the total alkaloid content in the black nightshade.

The invention discloses a method for testing the content of alkaloid in dendrobe. After a dendrobe dried product is crushed, alkaloid is extracted through alkaline chloroform water circumfluence, a nonionic surfactant Tween-80 is added into test liquid, and the content of alkaloid in the dendrobe is tested through bromocresol green acid dye colorimetry. In the system for testing alkaloid in dendrobe, a proper amount of Tween-80 is added to enable the extraction efficiency of bromocresol green-chloroform to be improved, so that the sensitivity in testing alkaloid in dendrobe is improved, and quick layering, simplicity and convenience in operation, good repeatability and stable testing result are achieved.

The invention belongs to the technical field of biological engineering, and relates to a high-temperature-resistant citric acid producing strain. The invention also relates to a mutagenesis screening method and an application method of the strain. The strain provided by the invention is classified and named as Aspergillus niger zjs-8 with the conservation number of CCTCC (China Center for Type Culture Collection) NO: M2011359. The method for obtaining the strain comprises: mutagenizing a starting strain by ion beam implantation, then coating the treated strain liquid on a screening flat plate containing bromocresol green, culturing at a high temperature, selecting single colony with a larger ratio of color changing circle to the colony diameter, and carrying out high-temperature fermentation to produce citric acid. The strain screened by the method can bear 42 DEG C high temperature, and has stable genetic character and higher acid producing level under the high-temperature condition, thereby producing a feasible way for energy-saving production of citric acid industry.

The invention relates to a method for detecting a concentration of a soft movable gel microsphere (SMG) of a deep oil displacement and profile control agent. The method comprises the steps of: preparing the SMG microsphere to have 4-8 standard concentrations by using in-place injection water, transferring 5ml of standard solution, adding 5-10g of digestion accelerator and 6-30mL of concentrated sulfuric acid, digesting at a digestion temperature of 400-500 DEG C for 1-2h; after the digestion is completed, cooling to reach room temperature, adding 40 percent sodium hydroxide, distilling to obtain inorganic ammonia, absorbing generated ammonia by using a 2 percent boric acid solution, using a methyl red-bromocresol green mixed indicator as an indicator, titrating an absorption solution by using standard hydrochloric acid to obtain a mmol number of consumed hydrochloric acid; and drawing a horizontal coordinate as the mmol number of the consumed hydrochloric acid and a vertical coordinate as an SMG concentration curve of the SMG concentration to obtain a linear regression equation, and figuring out the content of the SMG in an extraction solution by using the linear regression equation. The method can be used for accurately measuring the concentration of the SMG in the extraction solution.

The invention belongs to the field of zinc hydrometallurgy and particularly relates to a method for removing fluorine and chlorine in a zinc sulfate solution. The method comprises steps as follows: A, adjustment of a pH (potential of hydrogen) value; B, recovery of zinc; C, fluorine removal; D, chlorine removal. According to the method, a bromocresol green indicator is adopted to control the pH value properly, and the condition is easy to control; zinc is recovered, then fluorine and chlorine are removed sequentially, and operation is simple; the defects of poor fluorine removal effect of a current copper slag and silver slag dechlorinating method, great loss of zinc, difficulty in control of technological conditions and the like are effectively overcome, the requirements that the content of fluorine in electrolyte is smaller than 50 mg/L and the content of chlorine in the electrolyte is smaller than 300 mg/L at home and abroad are met, the cost is low, and the method is simple and easy to operate.

The invention relates to an acidity testing method for a sulfuric acid solution containing copper and iron. The method includes transferring the sulfuric acid solution containing the copper and the iron into a conical bottle, adding deionzied water into the bottle, then adding appropriate potassium oxalate, shaking the bottle up to enable the potassium oxalate to be dissolved completely, dropping 6-10 drops of bromocresol green-methyl red mixed indicator into the bottle, conducting titration through a sodium hydroxide standard solution, dropping two drops of sodium hydroxide standard solution in an excessive mode to reach the terminal point when the sulfuric solution changes from dark red to dark green in the titration process and acquiring the acidity of the sulfuric acid solution containing the copper and the iron according to an acid-base titration formula. The method can well solve the problem that the terminal point is not obvious in change in the original acid-base testing method, no solution precipitation is produced in the titration process, the titration terminal point is easy to judge, and the measured acidity is accurate.

A microbial type time-temperature indicator for low-temperature circulation items comprises a time-temperature indicator and a color comparison card. The time-temperature indicator comprises a container, wherein nutrition substrate, a potential of hydrogen (PH) indicating agent and microbes are stored in the container. The nutrition substrate is nutrient substance needed by growth of the microbes and is mixed by 10g/L dextrose, 2g/L ammonium sulfate and 5g/L NaCl solution according to the mass ratio of 1: 0.18-0.23: 0.47-0.52. The PH indicating agent is a methyl red and bromocresol green mixed indicating agent mixed by 72-77 wt% of bromine cresol green ethanol solution with concentration of 0.1% and 23-28 wt% of methyl red ethanol solution with concentration of 0.2%. The PH indicating agent and the nutrition substrate are mixed according to the mass ratio of 1: 98-103. The microbes are lactobacillus GG (LGG). The microbial type time-temperature indicator for the low-temperature circulation items utilizes the growth and metabolism of the LGG to produce acid and utilizes mixed methyl red and bromocresol green as the indicating agent so that variable color changes can be produced, and the purpose of indicating a time-temperature history experienced by foods is achieved.

The invention relates to a polybromocresol green modified glassy carbon electrode and application thereof, and mainly relates to the preparation of electro-polymerized bromocresol green modified glassy carbon electrode, and the application of the electrode in the sensitive quantitative analysis determination of uric acid, dopamine and ascorbic acid through the differential pulse voltammetry. The experimental result shows that in a phosphate buffered solution with the concentration of 0.1 mol/L and the pH of 6, the modified electrode has obvious catalysis function and sensibilization on uric acid, dopamine and ascorbic acid, and under the optimum condition, and through the adoption of the differential pulse voltammetry, the concentrations of uric acid, dopamine and ascorbic acid have better linear relations with the peak current thereof within the ranges of 0.5-15 micrometers, 0.08-4.0 micrometers and 5-150 micrometers, and the detection limits reach 0.17, 0.017 and 0.17 micrometer respectively; the method shows satisfactory results when the electrode is applied to determination of contents of uric acid, dopamine and ascorbic acid in plasma.

The invention relates to a method for titration of the zinc content in a silver-copper-zinc alloy by ethylenediamine tetraacetic acid (EDTA). The method includes: weighing a unit sample in a beaker, dissolving the sample in nitric acid, taking part of the sample solution into a triangular flask, and adding a thiourea solution to mask the interference of silver copper; adding a bromocresol green indicator, a hexamethylene tetramine solution and water to adjust the pH to about 5.0, adding a xylenol orange indicator, and using an ethylenediamine tetraacetic acid (EDTA) standard solution to perform titration till the solution just changes from purple to grass green, recording the milliliter number of the consumed standard solution, and calculating the percent content results of each to-be-measured element according to a calculation formula. The method provided by the invention simplifies operation steps, shortens the testing time, reduces the interference of other elements on the sample, improves the accuracy of the test result, and is convenient for operation.

The invention discloses test paper for semi-quantitative determination of ammonia concentration in the air, and a preparation method and a determination method thereof, and belongs to the technical field of environmental monitor. The test paper is loaded with bromocresol green, nonionic surfactants and acids, wherein the bromocresol green, the acids and the nonionic surfactants are in a ratio of (0.5-1.4) to (0.94-13.1)*10<-6> to (0.5-3)*10<-3>. The preparation method includes soaking filter paper or chromatography filter paper into prepared soaking liquid, and drying and plastically packagingthe filter paper or the chromatography filter paper to acquire the test paper for semi-quantitative determination of the ammonia concentration in the air. The determination method includes cutting off one end of the test paper for semi-quantitative determination of the ammonia concentration in the air, putting the test paper in a detection region, and after 7-30 minutes of reaction, acquiring theammonia concentration in the air of the detection region according to the change length of blue along the color-changeable test paper. The uniformity and the manufacture cost of the test paper are lower than the uniformity and the manufacture cost of a gas detector, and the test paper is convenient to use and has remarkable promotion and application value.

The invention relates to the technical field of immunodetection, and in particular relates to a fluorescent immunochromatographic test strip and a reagent card. The fluorescent immunochromatographic test strip comprises a bottom plate, and a sample pad, a fluorescent pad, a nitrocellulose membrane and a sample absorption pad are sequentially disposed on the bottom plate along the longitudinal direction of the bottom plate; the sample pad and the fluorescent pad partially overlap, the fluorescent pad and the nitrocellulose membrane partially overlap, one side of the fluorescent pad is located below the sample pad, and the other opposite side of the fluorescent pad is located above one side of the nitrocellulose membrane; the nitrocellulose membrane the and aspiration pad partially overlap,and the other opposite side of the nitrocellulose membrane is located below the sample absorption pad; the nitrocellulose membrane is provided with a detection line and a quality control line, and thedetection line is close to the fluorescent pad; the sample pad is treated with a sample pad treatment solution which contains 0.1% to 0.5% of a bromocresol green ethanol solution having a concentration of 10%. The fluorescent immunochromatographic test strip has high detection sensitivity and high detection efficiency and accuracy.

The invention relates to test paper for detecting the ammonia concentration in the air as well as preparation and a detection method thereof, and belongs to an environment monitoring technology. The test paper for detecting the ammonia concentration in the air is test paper loaded with bromocresol green, sulfuric acid and anionic surfactants, and is prepared from the following ingredients of bromocresol green, sulfuric acid and anionic surfactants according to the content ratio of (0.08 to 0.14):(0.38 to 0.94)*10<-4>:(0.04 to 0.5)*10<-3>. The preparation method comprises the following steps ofpreparing a soaking solution; putting the filter paper into the soaking solution to be soaked to obtain a soaked filter paper strip; performing encapsulation after drying or baking; obtaining the test paper for detecting the ammonia concentration in the air. One side of the test paper is sheared open and is put in a detection region; ammonia gas in the air in the detection region reacts with reagents in the test paper for detecting the ammonia concentration in the air; the color development length is read; the ammonia concentration in air in the detection region is obtained. The test paper has the advantages of high sensitivity, high accuracy, low manufacturing cost and simple operation.

The invention discloses and provides alcohol group casting coating with a dry-wet indication function and a sintering peeling function and a production process of the alcohol group casting coating. The fireproofing material of the coating comprises the following materials by weight: 40-50% of mullite powder, 30-40% of high bauxite powder, 20-30% of quartz sand powder; and the fireproof material comprises other materials by weight: suspending agent: 1-3% of hectorite powders and 1-3% of palygorskite powders; 5-10% of agglutinant; binding agent: 1-2% of thermoplastic phenol resins and 0.5-1.5% of rosins; 0.1-0.5% of bromocresol green indicator, 30-40% of industrial alcohol; and 10-20% of isopropyl alcohol. The production process of the alcohol group casting coating with the dry-wet indication function and the sintering peeling function is simple and convenient to operate; and the raw materials are abundant and the production cost is low. The alcohol group casting coating with the dry-wet indication function and the sintering peeling function and the production process of the alcohol group casting coating are suitable for the technical field of casting materials.

The invention discloses a determination method of the cation exchange capacity in soil. An ammonium acetate solution for air-dried soil sample is dissolved, uniform mixing is performed, a centrifugal machine is used for conducting centrifuging, and a supernate is taken; the ammonium acetate solution is used for repeated treatment; ethyl alcohol is added into lower-layer slurry, uniform mixing is performed, then the centrifugal machine is used for conducting centrifuging, the ethanol solution is removed, and the ethyl alcohol is used for repeated washing; after collection is performed, wax and magnesium oxide are added, distillation treatment is performed, a Nessler reagent is used for inspecting whether distillation is complete or not, a methyl red-bromocresol green mixing indication agent is added dropwise, and the hydrochloric acid standard solution is used for titration. According to the determination method, traditional methods are improved, the pretreatment and analysis steps are simplified, the sample treatment operation is more accurate in the whole determination process, and therefore the determination result is more accurate. The method has the advantages of being easy and convenient to operate, rapid, accurate and high in accuracy.

The invention relates to a Clostridium acetobutylicum strain capable of quickly generating butanol by fermenting xylose and starch, and a screening method and application thereof. The invention discloses Clostridium acetobutylicum, which is classified and named Clostridium acetobutylicumXY16 with the preservation and registration number of CCTCC NO:M2010011. The strain obtained by ion beam mutation and screening of a xylose plate, a 2-deoxy-D-glucose plate, a bromocresol green plate and a resazurin plate can quickly and efficiently generate the butanol by fermenting xylose and starch, has the advantages of high yield of total solvent, high butanol ratio and good repeatability, and is a good strain suitable for industrial production.

The invention discloses pH test paper for water quality testing and a water quality testing method. The pH test paper for water quality testing is prepared by soaking a carrier in a pH color-developing reagent, and drying at 50 to 60 DEG C for 10 to 40 minutes, wherein the pH color-developing reagent is a bromine thymol blue solution with the concentration being 0.7 to 0.8g/L, a phenol red solution with the concentration being 0.7 to 0.8g/L, or a bromocresol green solution with the concentration being 0.5 to 0.6g/L; the carrier is a chromatography test strip or chromatography filter paper. According to the pH test paper for water quality testing and the water quality testing method provided by the invention, pH of water quality can be quickly, conveniently and accurately detected without the need for using a special instrument, so that a detection range is wide; a bromine thymol blue and phenol red indicator developing in an alkaline zone but not developing in an acid zone is selectedfor detecting an alkaline part, and a bromocresol green indicator developing in the acid zone but not developing in the alkaline zone is selected for detecting an acid part, so that a detection resultis more accurate; phenol red pH test paper prepared by a color-developing reagent phenol red can be also used for carrying out quantitive pH detection.