The invention discloses a difunctional fluorescent probe based on a benzo [f] chromene derivative and application of the difunctional fluorescent probe in fish freshness indication and fluorescent dye, the
structural formula of the fluorescent probe is shown in the specification, and the probe can generate obvious colorimetric and fluorescent
dual response to various volatile amines in a water-containing medium, and has broad-spectrum recognizability, high sensitivity and wide pH adaptability. The two-channel visual indication
label is prepared by compounding the two-channel visual indication
label with
bromocresol green according to the ratio of 5: 1, the
label can turn green from yellow under
sunlight and turn green from orange red under
ultraviolet light after being contacted with amine steam, the two-channel color change of the indication label is highly synchronous with the TVB-N value change measured by the
national standard, and the three grades of'fresh, qualified and rotten 'of fish meat can be accurately distinguished; and the reliability and the accuracy of the method in practical application are verified. Besides, the probe can also be used as a fluorescent dye, can be prepared into
film coating liquid or ink, can be used for preparing luminous patterns and characters on glass,
silica gel plates,
filter paper and even flexible
polyvinyl alcohol (PVA) films in manners of scribbling,
stamping, dipping and the like, and shows application potentials in the fields of information recording, anti-counterfeiting and flexible photoelectric materials.