3 results about "Candidatus" patented technology

This invention relates to a device and method for rapidly starting a short-cut nitrification process, belonging to the field of water treatment. The invention involves inoculating a secondary sedimentation tank with sludge and adding a polyurethane biological sponge carrier into a sequencing batch reactor (SBR). First, a phased influent influent + incremental post-aeration strategy (increasing aeration time first, then increasing aeration rate) is adopted. This utilizes nitrate and a dynamic oxygen environment to drive the migration of *Candidatus Contendobacter* to the sponge carrier and complete its enrichment and acclimatization, reducing the obstruction of O2 on the endogenous denitrification process of *Candidatus Contendobacter* under aerobic conditions. The phased influent influent is then eliminated, and NH4 is introduced. + -N synthesis wastewater is treated using a low-alkalinity strategy (reducing influent KHCO3 concentration) + post-aeration with an initial increase followed by a decrease in aeration volume, combined with strong NO2 stimulation from Candidatus Contendobacter in a low dissolved oxygen environment. ‑ -N reducing power inhibits NOB growth, enabling rapid start-up of the short-cut nitration process.

The invention discloses a gamma-cyclodextrin glucosyltransferase mutant and application thereof, the mutant is obtained by mutating the 187th serine of wild gamma-CGTase from Candidate Angelobacter sp. Into alanine and mutating the 265th asparagine into phenylalanine, and the nucleotide sequence of the coding gene of the mutant is as shown in SEQ ID NO.7. The invention also discloses a preparation method of the gamma-cyclodextrin glucosyltransferase mutant. The invention also provides a recombinant expression vector containing the gene, a recombinant genetically engineered bacterium and application of the recombinant expression vector and the recombinant genetically engineered bacterium in preparation of gamma-cyclodextrin. Compared with wild gamma-CGTase, the relative enzyme activity of the mutant is improved by 1.8 times; after incubation at 60 DEG C for 2 hours, the residual enzyme activity is still kept at 85% or above; when the mutant is used for catalyzing starch to prepare gamma-cyclodextrin, the proportion of gamma-cyclodextrin in a product is not lower than 97%. Therefore, the gamma-cyclodextrin can be efficiently and highly selectively prepared only by taking the natural starch as the substrate, and a novel enzyme preparation and a process route which are efficient, stable and better in cost are provided for industrial production of the gamma-cyclodextrin.

The application discloses a bacterial strain which cannot be cultured alone Candidatus Bacillus cooperans CBS2, and a mixed fermentation system for improving the fermentation efficiency of anti-mildew subtilin by co-culturing CBS2 and bacillus subtilis BS-Z15. The preservation number of CBS2 is CCTCC NO: M 20251893, and the strain cannot be cultured alone. The preservation number of BS-Z15 is CCTCC NO: M 20251892. When the bacterial strain CBS2 which cannot be cultured alone is co-cultured with bacillus subtilis BS-Z15, the fermentation characteristics of BS-Z15 for producing anti-mildew subtilin can be changed extremely significantly, the yield peak of anti-mildew subtilin is brought forward from 60-80 hours to 18-26 hours, and the production efficiency is improved by several times. The application further provides a co-culturing system composed of the two strains, a method for efficiently producing anti-mildew subtilin by using the system and application of the method in the fields of agricultural biological control and the like. The application provides a disruptive technology for green and efficient production of antibiotics, and opens up a new way for developing microbial resources which cannot be cultured alone.