78 results about "Growth inhibitory" patented technology

Novel compositions are described, each comprising at least one active constituent that has a 12-carbon atom backbone as a 12-carbon chain base structure having at least a sub-terminal nitrogen and no terminal amino group. One of the active constituents is Boc-12-Ado-OH (BOA) and / or N,N-dimethyldodecylamine N-oxide (LO). Novel particle or liposomal compositions comprising at least BOA and / or LO may be prepared with an emulsifier and / or lipid and optionally a biopolymer, and may be formulated into pharmaceutically acceptable formulations and / or medicaments with or without one or more pharmaceutically acceptable carriers or excipients for utilization as an antimicrobial against one or more microbial pathogens. The novel antimicrobial compositions have broad-spectrum growth inhibitory or other destructive activity against microbial pathogens, biofilms produced by one or more pathogens, and biofilm infections.

The invention discloses bacillus amyloliquefaciens capable of resisting plant pathogenic bacteria and application of the bacillus amyloliquefaciens, and belongs to the technical field of microorganisms, the bacillus amyloliquefaciens is classified and named as bacillus amyloliquefaciens KT-7, the bacillus amyloliquefaciens KT-7 is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on June 9, 2020, and the address is No.66, Courtyard No.100, Xianlie Middle Road, Guangzhou City, Guangdong Province; the preservation number of the strain is GDMCC No: 61051. The bacillus amyloliquefaciens provided by the invention has a growth inhibition effect on plant pathogenic bacteria, and particularly has a good control effect on bacterial diseases such as citrus canker and tomato bacterial wilt.

This invention discloses a stress-resistant and yield-increasing plant growth regulator, its preparation method, and its application, belonging to the field of plant growth regulators. The stress-resistant and yield-increasing plant growth regulator of this invention comprises the following raw materials: *Suaeda salsa* extract, a stress-resistant biostimulant, propionyl brassinolide, styracil, polyaspartic acid, γ-aminobutyric acid, emulsifier, penetrant, co-emulsifier, pH buffer, and deionized water; wherein the stress-resistant biostimulant is perilla lactone. This invention significantly improves the tolerance of wheat to salt-alkali stress by strengthening the antioxidant system and osmotic regulation capacity, ensuring its basic survival and normal physiological functions; and effectively reverses stress-induced growth inhibition through mechanisms such as promoting root development, enhancing photosynthesis, and driving product translocation to grains, thereby achieving a significant yield increase under adverse conditions.

The application discloses application of MdERF77 gene in plant resistance to saline-alkali stress, and belongs to the technical field of biotechnology. The application overexpresses the MdERF77 gene in an apple plant through transgenic technology, significantly enhances the tolerance of the plant to saline-alkali stress, and has outstanding technical effects. The method can effectively improve the growth adaptability of the apple in a saline-alkali environment, so that the transgenic plant shows stronger antioxidant capacity, higher photosynthetic efficiency and more stable membrane system under the saline-alkali stress, thereby guaranteeing normal development and quality improvement of fruits, and reducing growth inhibition and yield loss caused by stress. The application provides a reference for improvement of stress resistance of fruit trees, and has a wide agricultural application prospect.

The invention discloses a cyclic iridium-ester tin imidazo phenanthroline complex with an aggregation-induced emission (AIE) characteristic as well as a preparation method and an anti-cancer application of the cyclic iridium-ester tin imidazo phenanthroline complex. The structural formula is as shown in formula (I), R1 is phenyl or 3-pyridyl, and R2 is hydrogen or fluorine. By testing the growth inhibition rate of the target compound on human alveolar basal epithelial cancer cells (A549) and comparing, the target compound shows potential A549 cell proliferation inhibition activity. In addition, the target complex shows a unique AIE luminescence characteristic, and is accumulated in lysosome of A549 cells, resulting in lysosome damage and apoptosis.

Novel compositions are described, each comprising at least one active constituent that has a 12-carbon atom backbone as a 12-carbon chain base structure having at least a sub-terminal nitrogen and no terminal amino group. One of the active constituents is Boc-12-Ado-OH (BOA) and / or N,N-dimethyldodecylamine N-oxide (LO). Novel particle or liposomal compositions comprising at least BOA and / or LO may be prepared with an emulsifier and / or lipid and optionally a biopolymer, and may be formulated into pharmaceutically acceptable formulations and / or medicaments with or without one or more pharmaceutically acceptable carriers or excipients for utilization as an antimicrobial against one or more microbial pathogens. The novel antimicrobial compositions have broad-spectrum growth inhibitory or other destructive activity against microbial pathogens, biofilms produced by one or more pathogens, and biofilm infections.

The invention discloses application of a traditional Chinese medicine composition in preparation of a medicine for preventing and treating bacterial liver injury of chicks. The traditional Chinese medicine composition is prepared from herba artemisiae scopariae and radix scutellariae. For bacterial infection and immunological liver injury triggered by endotoxin, through compatibility and synergy of'one clearing and one benefiting 'of two medicines, pathogen stimulation and immune overresponse links are intervened at the same time, and growth inhibition caused by liver injury is blocked. The traditional Chinese medicine composition is extracted and prepared into oral liquid, powder and other dosage forms, and then is applied to chicks at the age of 6-12 days. Experiments prove that the traditional Chinese medicine composition can significantly reduce the activity of liver injury markers such as serum ALT and AST, alleviate liver inflammatory infiltration and necrosis, can reverse the decrease of average daily gain caused by liver injury, synchronously realizes liver injury repair and growth performance guarantee, adapts to resistance reduction / forbidden resistance trends, and has a broad application prospect. And a safe and effective solution is provided for preventing and treating bacterial liver injury in the early brooding stage of broilers.

The application discloses methyl phenolic compounds, a preparation method and application thereof. Six new methyl phenolic compounds are separated from the skin of eleocharis tuberosa, and the compounds all show good inhibitory activity on alpha-glucosidase, and are stronger than the clinical first-line drug acarbose. The inhibitory activity of compound 3 is the best, enzyme kinetics research shows that the compound 3 plays a role as a reversible mixed inhibitor, ADME predicts that the compound 3 has good drug properties, including high gastrointestinal absorption and no CYP450 enzyme inhibition. The cytotoxicity experiment shows that the six compounds have no obvious growth inhibition on human embryonic kidney cells HEK293 at a concentration of 100 muM, and have good safety. The compounds can be combined with a pharmaceutically acceptable carrier to form a pharmaceutical composition, and are used for preparing a health care product for reducing blood sugar or a drug for treating diabetes. The preparation method is simple, easy to operate, suitable for industrial production, and is helpful to the high-value reuse of the skin of eleocharis tuberosa.

The invention discloses a method for identifying nitrogen absorption capacity of wheat in a seedling stage based on potassium chlorate culture in combination with field experiments. The method comprises the following steps: (1) screening potassium chlorate with proper concentration; (2) after the seeds of the different wheat varieties germinate, transplanting the seeds into a culture medium containing potassium chlorate with a proper concentration, culturing the seeds to a one-leaf and one-core period, and calculating the growth inhibition ratio of the different wheat varieties so as to evaluate the sensitivity of the different wheat varieties to the potassium chlorate sensitivity; (3) field trials are adopted at the same time, crops of different wheat varieties are planted randomly, the trials take nitrogen fertilizer as main treatment, and three nitrogen element levels are designed in total and are respectively a group without nitrogen fertilizer application, a group with normal nitrogen application and a group with high nitrogen; and in the test process, observing and recording agronomic characters of each test material. According to the method, potassium chlorate screening in the seedling stage is combined with field agronomic character single plant grain weight growth rate comprehensive evaluation, and the efficient nitrogen absorption germplasm can be accurately identified.

This invention belongs to the field of plant genetic engineering and plant stress resistance biotechnology, and discloses... ZmCYP81Q32 Application of genes in improving nicosulfuron resistance in plants. This invention provides... ZmCYP81Q32 The nucleotide sequence of the gene, as shown in SEQ ID NO:1, encodes a cytochrome P450 enzyme that can directly bind to sulfonylurea herbicides and promote their hydroxylation metabolism. This reduces the accumulation of the parent herbicide in the plant, alleviates herbicide-induced growth inhibition, leaf chlorosis, and oxidative damage, and significantly improves plant tolerance to sulfonylurea herbicides, especially enhancing plant resistance to nicosulfuron. This invention can be widely used for crop herbicide resistance improvement, herbicide-tolerant germplasm creation, and related molecular breeding.

The invention discloses a windmill-shaped alpha-Fe2O3 / Ag nano hybrid material and a preparation method and application thereof in relieving plant salt stress, and the method comprises the following steps: mixing fusiform alpha-Fe2O3 prepared by a forced hydrolysis method with silver acetate, then carrying out ball milling treatment, and then carrying out a high-temperature calcination process to obtain the windmill-shaped alpha-Fe2O3 / Ag nano hybrid material. And the windmill-shaped alpha-Fe2O3 / Ag hybrid structure formed by assembling the fusiform alpha-Fe2O3 and the Ag nanoparticles is obtained. The nano hybrid material has high specific surface area, excellent dispersity and remarkable leaf surface adhesion performance, when the nano hybrid material is applied to plant cultivation, the material can effectively relieve growth inhibition caused by salt stress, can regulate and control plant hormone signals, terpene and carbohydrate metabolism, sulfur metabolism, protein homeostasis and the like, realizes multi-channel comprehensive regulation, and has a wide application prospect. Therefore, the plant stress resistance and quality are improved.

The present invention addresses the problem of providing a highly safe and continuously ingestible agent for: preventing or ameliorating diseases attributed to microorganisms belonging to the family Peptostreptococcaceae by inhibiting the growth of these microorganisms in the intestine; or for preventing or ameliorating diseases potentially preventable or ameliorable by a substance produced from microorganisms belonging to the genus Slackia by promoting the growth of these microorganisms in the intestine.　The problem of the present invention has been solved by a growth inhibitor of microorganisms belonging to the family Peptostreptococcaceae or a growth promotor of microorganisms belonging to the genus Slackia, each containing a galactooligosaccharide as an active ingredient.

The invention discloses a programmed function switching-based calcium sulfate whisker length-diameter ratio accurate regulation and control method, and belongs to the technical field of inorganic functional crystal material preparation. The traditional static regulation and control limitation is broken through, and a new mechanism of programmed adsorption-desorption function switching is created for the first time. The preparation method comprises the following steps: constructing an initial growth inhibition template by using amino trimethylene phosphonic acid (ATMP) as a crystal face adsorbent; in different stages of crystal growth, desorption agents with complementary functions are introduced in stages: sodium citrate is introduced in a competitive growth period, and radial growth inhibition is partially relieved; polyvinyl alcohol (PVA) is introduced in the length-diameter ratio curing period, and material transmission at the axial growth end is specifically promoted. The function switching process is precisely coupled with a gradient temperature change process, and sequential dynamic reconstruction of a crystal growth interface is realized. According to the invention, the length-diameter ratio of the calcium sulfate whisker is regulated and controlled in an ultra-wide range of 50-500, the product uniformity is improved, and a raw material with better performance is provided for the fields of high-end composite materials and the like.

This invention discloses a kit for constructing an iron-dependent growth environment for Acinetobacter baumannii and its application method, belonging to the field of biotechnology. The kit is specifically designed for Acinetobacter baumannii, and its core components include a 50 mmol / L sterile stock solution tube of 2,2′-bipyridine, a 2000 μmol / L sterile stock solution tube of FeCl3, and a user manual. It does not include general-purpose LB medium, thus avoiding the problem of high-temperature and high-pressure deterioration of core reagents and highlighting the innovative combination of dedicated reagents. This invention also provides the application method of the kit. By adding the kit stock solution to the autoclaved and cooled LB medium in a specific ratio, standardized growth environments with normal iron, iron deficiency, and iron enrichment can be rapidly constructed. Dynamic monitoring and verification of the reversibility of growth inhibition-recovery ensure that the iron environment is precise and controllable. The kit is easy to operate, has good reproducibility, and can achieve standardized construction of the iron environment for Acinetobacter baumannii. It is suitable for iron metabolism-related research such as functional analysis of iron uptake-related proteins, development of iron deprivation antibacterial strategies, and screening of iron-deficient strains.

The application belongs to the technical field of natural compound application, and particularly relates to application of globulol in preparation of lysine-deficient Staphylococcus aureus. The application provides, for the first time, application of globulol in preparation of lysine-deficient Staphylococcus aureus. Globulol in the application is a natural monomer component in eucalyptus, and is a natural sesquiterpene compound. After endogenous lysine is consumed by pre-treatment according to the application, S. aureus Globulol has obvious growth inhibition effect on Staphylococcus aureus in a lysine-deficient culture system, and the growth inhibition phenomenon is obviously recovered after exogenous lysine is supplemented under the same control.

This invention belongs to the field of biotechnology, specifically relating to the cassava transcription factor MeWRKY30 and its applications. This invention cloned the transcription factor gene of MeWRKY30 from cassava. Overexpression of this gene in cassava can improve the resistance of transgenic cassava plants to low-temperature and drought stress. The effects of qRT-PCR on cassava... MeWRKY30 Gene expression was significantly upregulated after drought and treatment with the plant hormone abscisic acid (ABA). After recovery from low temperature treatment, the malondialdehyde content of the overexpressing cassava lines decreased, wilting was reduced, root growth inhibition was lessened, and the number of cortical cells in the root tip meristem was significantly increased.

The invention relates to the technical field of microalgae culture, and discloses a microalgae culture method. The invention relates to a microalgae culture method, which comprises the following steps: (1) in the presence of a fat degradation inhibitor, culturing microalgae species to obtain a microalgae solution; (2) separating, adsorbing and degerming the algae solution to obtain a culture solution; and (3) returning the culture solution to the step (1) for reuse. The method can effectively control the generation of organic matters with a growth inhibition effect in the microalgae culture process, improve the production rate and reduce the culture water consumption.

A step of preparing a base substrate of a single crystal of a group III nitride semiconductor; a growth inhibition layer forming step of performing in situ formation of a growth inhibition layer over the entire main surface of the base substrate in a vapor phase growth apparatus; a first step of growing a first layer by epitaxially growing a single crystal of a group III nitride semiconductor on the main surface of the base substrate via openings in the growth inhibition layer by using the vapor phase growth apparatus where the base substrate on which the growth inhibition layer has been formed is placed in the vapor phase growth apparatus; and a second step of growing a second layer with a mirror surface by epitaxially growing a single crystal of a group III nitride semiconductor on the first layer so as to make the inclined interfaces disappear.

The spirogyra growth restraining device comprises a floating platform, a plurality of circular parabolic tables connected together through a connecting frame are arranged above the floating platform, the diameters of the circular parabolic tables are sequentially increased from bottom to top, a parabolic opening is formed in the side wall of each circular parabolic table, and the circular parabolic tables are arranged on the floating platform. One or more than two centrifugal push plates are arranged in each floating platform, the floating platform is provided with a driving mechanism for controlling the centrifugal push plates to rotate, the driving mechanism comprises a motor, the motor is fixedly connected with the floating platform, and the output end of the motor is fixedly connected with an outer sleeve. According to the utility model, through the cooperation of the multi-layer circular parabolic table and the centrifugal push plate, the slow-release balls wrapped with the bamboo powder leavening can be automatically thrown into water in different ranges, and when the slow-release balls fall onto spirogyra at the water bottom, the bamboo powder leavening can slowly release allelochemicals to inhibit the growth of the spirogyra, so that not only is the time saved, but also the labor cost is reduced.

The application relates to a cultivating method of salt-tolerant bacteria and agricultural application, and the cultivating method comprises the following steps: S1, mixing straw, mushroom cultivation waste, poultry manure, inorganic salt and culture medium matrix to obtain a biological waste-based culture medium; S2, respectively culturing pythium and bacillus by using the biological waste-based culture medium to obtain a compound bacterial strain liquid; S3, respectively mixing diatomite and the compound bacterial strain liquid, adsorbing and drying to obtain an intermediate product; S4, blending chitosan, phytanic acid and an activator, and reacting to obtain hydrophobic chitosan; and S5, blending and stirring the intermediate product and the hydrophobic chitosan to obtain salt-tolerant bacteria. The hydrophobic chitosan is beneficial to stable storage of the salt-tolerant bacteria in a high-humidity environment, reduces adverse effects such as growth inhibition between different salt-tolerant bacteria, and makes different salt-tolerant bacteria play a synergistic role, so that the efficiency of the fertilizer is finally improved.

A method and kit for inhibiting microbial proliferation in a fuel container are disclosed. The method involves introducing antimicrobial particles designed to be trapped at the fuel-water interface by interfacial tension, thereby concentrating antimicrobial agents at a primary site of microbial growth. These particles release antimicrobial ions into the water phase, creating a growth inhibition zone that can extend into the sludge zone. A kit is also provided, including at least two separate containers of particles being a denser type that settles and remains at the tank bottom and a lighter type that settles but is subsequently lifted to the interface upon water accumulation.

A method and kit for inhibiting microbial proliferation in a fuel container are disclosed. The method involves introducing antimicrobial particles designed to be trapped at the fuel-water interface by interfacial tension, thereby concentrating antimicrobial agents at a primary site of microbial growth. These particles release antimicrobial ions into the water phase, creating a growth inhibition zone that can extend into the sludge zone. A kit is also provided, including at least two separate containers of particles being a denser type that settles and remains at the tank bottom and a lighter type that settles but is subsequently lifted to the interface upon water accumulation.

The invention belongs to the technical field of tea tree pest control, and relates to an application of linalool in control of ectropis grisescens, the linalool plays a role in a mode of directly feeding the ectropis grisescens or a mode of treating the ectropis grisescens through gaseous exposure, the linalool is used as a unique inhibiting or lethal component, and the linalool can be used for preventing and treating the ectropis grisescens. The compound has a remarkable growth inhibition or lethal effect on ectropis grisescens. When the concentration of the linalool is 5-20 [mu] l / mL, the linalool can generate a remarkable lethal effect on larvae of ectropis grisescens; when the concentration of linalool is 0.2-2 [mu] l / mL, the growth rate of the linalool can be obviously inhibited, and the weight of the linalool can be reduced. When the ectropis grisescens is treated in a gaseous exposure mode, the exposure concentration is 70-90 ng / h, the treatment time is 4-6 days, and the control effect is remarkable. The linalool is used for preventing and controlling tea tree ectropis grisescens, and the method has the advantages of being efficient, safe, high in feasibility and the like and can be applied to comprehensive prevention and control of tea garden insect pests.

The invention belongs to but not limited to the technical field of biological control of plant diseases, and discloses bacillus velezensis BV1 and application thereof in enhancing the resistance of wheat to basal stem rot. The bacillus velezensis BV1 provided by the invention can inhibit the growth of fusarium pseudograminearum, and the inhibition rate is 66.67%. The BV1 can enable hyphae of the fusarium pseudograminearum to expand, deform and generate apoptosis. According to the invention, the mechanism of inducing the apoptosis of the fusarium pseudograminearum by the BV1 is analyzed. Meanwhile, the main pathogenic factor RPL22 in the fusarium pseudograminearum is found. A foundation is laid for developing RPL22 gene silencing biological agents to reduce toxins generated by the fusarium pseudograminearum, so that the wheat yield and the food safety are guaranteed.

The invention discloses a foliage spraying agent as well as a preparation method and application thereof, and relates to the technical field of agricultural planting. The foliage spraying agent provided by the invention comprises the following raw materials: polyethyleneimine and a water-soluble silicon fertilizer, wherein the addition amount of the polyethyleneimine in the foliage spraying agent is 0.0005 g / L to 0.008 g / L, and the addition amount of the water-soluble silicon fertilizer in the foliage spraying agent is 0.5 mL / L to 5 mL / L; the water-soluble silicon fertilizer is a liquid fertilizer containing nano silicon. And correspondingly. The invention also discloses application of the foliar spraying agent in relieving cadmium toxicity of plants in a cadmium stress environment. The foliage spraying agent provided by the invention can relieve the toxicity of heavy metal cadmium toxicity to plants such as rice, Chinese cabbage and the like, so that the growth inhibition effect of cadmium stress on the plants is reduced, and the biomass and yield of the plants under cadmium toxicity growth are improved.

The invention relates to the technical field of environmental microbiological technology and agricultural bioremediation, and discloses a rhodococcus bacterium X-20 and application thereof. The Rhodococcus sp. X-20 can efficiently degrade various pesticides in liquid and soil environments, including carbendazim, dinotefuran, atrazine, pendimethalin and cyhalothrin, and especially shows specific efficient degradation ability to carbendazim. In addition, the strain has various plant probiotic functions: the strain can secrete indole-3-acetic acid (IAA), generate iron carriers, dissolve inorganic phosphorus and antagonize phytophthora nicotianae, and the growth of tobacco seedlings can be remarkably promoted. Field experiments show that the strain can relieve growth inhibition of compound pesticide pollution on tobacco, and the strain can be used for preparing microbial agents and applied to bioremediation of pesticide-polluted soil and green agriculture.

The invention discloses a culture method of an acetoin engineering strain and a strain construction method. The method comprises the following steps: exogenously adding a beneficial factor L-arginine into a corynebacterium glutamicum basic culture medium and / or strengthening an arginine endogenous synthesis route to construct corynebacterium glutamicum; the yield of acetoin produced by corynebacterium glutamicum is improved by exogenously adding arginine or improving the synthesis flux of arginine. L-arginine is added into the culture medium, so that a good permeation protection effect is provided for corynebacterium glutamicum, continuous growth and product synthesis of corynebacterium glutamicum under stress of high-concentration acetoin are promoted, and the yield of acetoin is increased; by enhancing the endogenous synthesis route of the arginine of the strain, the metabolic engineering transformation of acetoin production is improved, a metabolic network is finely regulated and controlled, growth inhibition and carbon loss caused by unfavorable distribution of metabolic flow are avoided, and the strain accumulates a small amount of arginine to promote acetoin production. An industrial basis is provided for producing acetoin through microbial fermentation, and the method has a good application prospect.

The invention relates to an aporphine-benzylisoquinoline alkaloid dimer as well as a preparation method and application thereof, and belongs to the field of natural medicines and biological medicines. The aporphine-benzylisoquinoline alkaloid dimer has a structural general formula shown in the specification, in the formula, R1, R2, R3, R4, R5, R6, R7 and R8 are independently selected from hydrogen, methoxyl, hydroxyl or two adjacent substituent groups are methylenedioxy, so that a five-membered ring is formed; and R9 is selected from hydrogen, methoxyl or hydroxyl. Six novel aporphine-benzylisoquinoline alkaloid dimers are extracted and separated from roots of thalictrum omeiensis, and through detection, the aporphine-benzylisoquinoline alkaloid dimers have obvious growth inhibition effects on human acute promyelocytic leukemia cells HL-60 and human prostate cancer cells PC-3, and have the prospect of preparing medicines for preventing and treating tumors.

The invention discloses a nisin titer detection method based on a microwell plate method. According to the growth inhibition effect of nisin on indicator bacteria, a plant lactobacillus L30 with a good strain growth amount-nisin titer curve is screened from existing strains, the plant lactobacillus L30 is applied to a microwell plate method, and a nisin titer determination method which is high in accuracy, convenient and economical is developed.

The present application relates to a kind of harmless physical method and reagent for treating corn borer.The reagent includes the following mass percentage components: colloid 0.2%-0.6%, diatomite 1%-2%, Bacillus thuringiensis 0.2%-0.3%, Beauveria bassiana 0.1%-0.15%, azadirachtin 0.03%-0.1%, and the balance is water.The present application uses the viscous colloid formed by colloid to effectively occupy corn bell mouth and stick to the corn borer that crawls out to feed, achieving physical control;At the same time, Bacillus thuringiensis and Beauveria bassiana biological components are used to infect and kill the corn borer, azadirachtin plays a role in repelling and growth inhibition, and diatomite can physically pierce the insect skin, enhancing the control effect, and through the synergistic effect of multiple components, biological control is achieved.The present application creatively uses harmless physical method combined with biological components to control corn borer, not only avoiding pesticide residues, but also being more environmentally friendly and safe, and synergistically enhancing the control effect, reducing the worm boring rate.