111 results about "Growth inhibitory" patented technology

The invention provides a bacillus velezensis strain and application thereof. The name of the bacillus velezensis is BJ-1141, and the bacillus velezensis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.32094. The strain has broad-spectrum antibacterial activity, and has a good inhibition effect on 24 plant pathogenic fungi such as rice pathogenic bacteria, wheat pathogenic bacteria, corn pathogenic bacteria, grape pathogenic bacteria, cherry pathogenic bacteria, solanaceous crop pathogenic bacteria and the like. Wherein the growth inhibition effect on sclerotinia sclerotiorum hyphae causing eggplant sclerotinia rot is optimal, and the inhibition rate can reach 95.57%. The BJ-1141 bacterial liquid has an obvious growth promoting effect on the growth of plants such as tomatoes and lettuce. After the BJ-1141 bacterial liquid is used for irrigating roots of potted tomatoes, the tomato fusarium wilt caused by tomato fusarium oxysporum can be effectively controlled, the BJ-1141 bacterial liquid has a good prevention and treatment effect, the prevention and treatment effect can reach 85.93%, and the prevention and treatment effect is obviously different from that of a control group.

The invention belongs to the technical field of microorganisms, and discloses a Lactobacillus paracasei B20 for inhibiting melanin from a plateau area and application of the Lactobacillus paracasei B20, the Lactobacillus paracasei B20 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.32434, and the preservation date is October 31, 2024. Metabolite of the lactobacillus paracasei B20 comprises phenylalanine, p-hydroxycinnamic acid and the like, has strong tyrosinase inhibition effect and anti-ultraviolet ability, and can significantly reduce generation of melanin in skin. In addition, the lactobacillus paracasei B20 also has relatively strong oxidation resistance, has good tolerance in artificial gastric juice and artificial intestinal juice, and can smoothly reach the intestinal tract of a human body. In addition, the lactobacillus paracasei B20 has a relatively obvious growth inhibition effect on skin conditioned pathogens such as staphylococcus aureus and enterococcus faecalis.

The invention discloses application of biological silver nanoparticles synthesized based on bacillus velezensis in prevention and treatment of tomato gray mold, and relates to the technical field of prevention and treatment of tomato gray mold. Researches find that the biological silver nanoparticles Bio-AgNPs have a relatively strong inhibition effect on the growth of botrytis cinerea hyphae, and when the concentration of the biological silver nanoparticles Bio-AgNPs is 100 mu g / mL, the growth inhibition rate of the botrytis cinerea hyphae is 100%. The Bio-AgNPs shows a good prevention and treatment effect on the tomato gray mold, after 40 [mu] g / mL of Bio-AgNPs is used for treating tomato fruits and leaves, the scab of the tomato fruits is obviously reduced by 52.21%, and the prevention and treatment effect on the tomato leaf gray mold is 40.19%.

Using chemical genetic screening, we discovered a small molecule Cellulosin (aka endosidin20 or ES20) that causes cell swollen and inhibits plant growth, but does not disrupt global vesicle trafficking. By doing mutant screening, we obtained multiple alleles of Arabidopsis thaliana that are resistant to Cellulosin inhibition in growth. Those mutated amino acid residues are conserved across plant species. Cellulosin targets a group of cellulose synthases (CesAs) of Arabidopsis thaliana by binding to a conserved domain essential for the catalytic activity of CesA. Cellulosin may target and inhibit all subtypes of CesAs in plants. The present invention relates to Cellulosin, a cellulose synthase inhibitor, its analogs or derivatives as a broad-spectrum herbicide. The mutated genes, their protein products and a cell or a plant having those mutated genes or expressing those protein products are within the scope of this disclosure.

The present invention belongs to the field of plant growth regulators, and specifically relates to a fruit coloring composition, a fruit coloring functional fertilizer and their applications. The fruit coloring composition comprises 1-aminocyclopropane carboxylic acid and a protective auxiliary agent, and the mass ratio of 1-aminocyclopropane carboxylic acid to the protective auxiliary agent is 1:0.1 to 200; the protective auxiliary agent is selected from 5-aminolevulinic acid, diethyl aminoethyl hexanoate or choline chloride. The present invention first discloses the use of protective auxiliary agents such as 5-aminolevulinic acid, which can effectively improve the adverse effects when ACC is applied alone, such as high dosage for coloring, easy to cause negative phenomena such as plant defoliation, fruit cracking, fruit drop and quality decline. When the effective application dosage is only 0.05 to 2.5 ppm, excellent coloring effect can be achieved. At the same time, the safety of exogenous application of ACC is effectively improved, and the planting effect of fruit and vegetable crops can be improved in aspects such as preventing fruit drop, increasing fruit sweetness, reducing phytotoxicity, and relieving the growth inhibition of stems, leaves and fruits.

The invention relates to the technical field of cadmium pollution control. The invention provides a method for relieving soybean cadmium stress by using 2, 4-epibrassinolide and a method for comparing the relieving effect of 2, 4-epibrassinolide on soybean cadmium stress, and the method comprises the following steps: spraying 2, 4-epibrassinolide with the concentration of 0.1-1.0 [mu] mol / L on soybean leaves, and continuously spraying for 2-5 days. Soybean varieties with large cadmium sensitivity difference are adopted for water culture test, cadmium treatment is performed on seedlings of the two varieties, 2, 4-epibrassinolide pretreatment is performed at the same time, the influence of 2, 4-epibrassinolide pretreatment on growth inhibition and physiological characteristics of cadmium stress of the soybean seedlings is studied, and the cadmium stress resistance of the soybean seedlings is improved. Potential effects of response and resistance mechanisms of plants under cadmium stress in Cd tolerance are discussed, and a theoretical basis is provided for cultivation of cadmium-tolerant and low-cadmium-accumulation soybean varieties.

One embodiment of the present invention provides a psicose epimerase expression cassette comprising a polynucleotide encoding a psicose epimerase and a promoter operably linked thereto and modulating the expression of the psicose epimerase in a Corynebacterium strain. The promoter consists of a base sequence as shown in a sequence 1, a base sequence as shown in a sequence 9, a base sequence as shown in a sequence 10 or a base sequence as shown in a sequence 11. The psicose epimerase expression cassette disclosed by the embodiment of the invention is almost not started in a normal environment for culturing a recombinant corynebacterium strain, and does not induce growth inhibition of the recombinant corynebacterium strain. Therefore, the recombinant corynebacterium strain transformed by the psicose epimerase expression cassette of one embodiment of the present invention can normally grow and proliferate to the logarithmic phase, and stably and highly express psicose epimerase after the logarithmic phase.

The invention provides a lactobacillus plantarum 18 which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No.31277. The lactobacillus plantarum 18 has the advantages that the lactobacillus plantarum 18 is prepared from lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum and lactobacillus plantarum; the plant lactobacillus 18 can inhibit growth of propionibacterium acnes, inhibit expression of virulence factors (biological membranes, lipase and the like) of the propionibacterium acnes, relieve inflammation, inhibit autophagy of cells, scavenge free radicals and inhibit activity of tyrosinase, so that the plant lactobacillus 18 has the effects of resisting acnes, dermatitis and oxidation and whitening skin. The plant lactobacillus 18 or the metabolite of the plant lactobacillus 18 can be used for preparing products with the effects or effects.

Novel compositions are described, each comprising at least one active constituent that has a 12-carbon atom backbone as a 12-carbon chain base structure having at least a sub-terminal nitrogen and no terminal amino group. One of the active constituents is Boc-12-Ado-OH (BOA) and / or N,N-dimethyldodecylamine N-oxide (LO). Novel particle or liposomal compositions comprising at least BOA and / or LO may be prepared with an emulsifier and / or lipid and optionally a biopolymer, and may be formulated into pharmaceutically acceptable formulations and / or medicaments with or without one or more pharmaceutically acceptable carriers or excipients for utilization as an antimicrobial against one or more microbial pathogens. The novel antimicrobial compositions have broad-spectrum growth inhibitory or other destructive activity against microbial pathogens, biofilms produced by one or more pathogens, and biofilm infections.

The present invention relates to a composition for inhibiting hair loss, promoting new hair growth, or promoting hair growth, comprising a tubulin inhibitor as an active ingredient. Also, the present invention relates to a pharmaceutical composition for inhibiting hair loss, promoting new hair growth, or promoting hair growth, comprising a tubulin inhibitor as an active ingredient. Also, the present invention relates to a quasi-drug composition for inhibiting hair loss, promoting new hair growth, or promoting hair growth, comprising a tubulin inhibitor as an active ingredient. Also, the present invention relates to a cosmetic composition for inhibiting hair loss, promoting new hair growth, promoting hair growth, and alleviating symptoms of hair loss, comprising a tubulin inhibitor as an active ingredient. Also, the present invention relates to a hair or scalp product comprising a tubulin inhibitor as an active ingredient. When the tubulin inhibitor of the present invention was applied to an animal model, the animal model was confirmed to exhibit significantly increased new hair growth, hair loss inhibition, and hair growth promotion effects, compared to a control. Therefore, the tubulin inhibitor of the present invention can be effectively used as a medicine or cosmetic product for hair loss or hair growth.

The invention discloses bacillus amyloliquefaciens capable of resisting plant pathogenic bacteria and application of the bacillus amyloliquefaciens, and belongs to the technical field of microorganisms, the bacillus amyloliquefaciens is classified and named as bacillus amyloliquefaciens KT-7, the bacillus amyloliquefaciens KT-7 is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on June 9, 2020, and the address is No.66, Courtyard No.100, Xianlie Middle Road, Guangzhou City, Guangdong Province; the preservation number of the strain is GDMCC No: 61051. The bacillus amyloliquefaciens provided by the invention has a growth inhibition effect on plant pathogenic bacteria, and particularly has a good control effect on bacterial diseases such as citrus canker and tomato bacterial wilt.

This invention discloses a stress-resistant and yield-increasing plant growth regulator, its preparation method, and its application, belonging to the field of plant growth regulators. The stress-resistant and yield-increasing plant growth regulator of this invention comprises the following raw materials: *Suaeda salsa* extract, a stress-resistant biostimulant, propionyl brassinolide, styracil, polyaspartic acid, γ-aminobutyric acid, emulsifier, penetrant, co-emulsifier, pH buffer, and deionized water; wherein the stress-resistant biostimulant is perilla lactone. This invention significantly improves the tolerance of wheat to salt-alkali stress by strengthening the antioxidant system and osmotic regulation capacity, ensuring its basic survival and normal physiological functions; and effectively reverses stress-induced growth inhibition through mechanisms such as promoting root development, enhancing photosynthesis, and driving product translocation to grains, thereby achieving a significant yield increase under adverse conditions.

The invention discloses application of an aromatic hydrocarbon receptor stimulant in preparation of a medicine for treating diarrhea of weaned piglets and / or inhibiting inflammation, and belongs to the field of biological medicine. According to the present invention, with the PAS-B structural domain of the AHR as the target, the virtual screening technology, the target verification test and the weaned piglet diarrhea pharmacodynamic evaluation test are performed to determine the arctigenin as the AHR strong agonist and the anti-inflammatory drug, and the arctigenin has the good treatment effect on the weaned piglet diarrhea. Experimental results show that 10 mg / kg of arctigenin can significantly relieve diarrhea and hemafecia symptoms of weaned piglets, reduce the content of inflammatory factors in serum and intestinal tissues and improve the intestinal structure of weaned piglets, has no significant cytotoxicity, hepatorenal toxicity and intestinal epithelial cell growth inhibition effect, and can be used for preparing a feed additive for weaned piglets. The compound can be used for preparing medicines for treating diarrhea of weaned piglets. The invention provides a new raw material medicine for treating diarrhea of weaned piglets, and has great economic value and wide application prospect.

The application discloses application of MdERF77 gene in plant resistance to saline-alkali stress, and belongs to the technical field of biotechnology. The application overexpresses the MdERF77 gene in an apple plant through transgenic technology, significantly enhances the tolerance of the plant to saline-alkali stress, and has outstanding technical effects. The method can effectively improve the growth adaptability of the apple in a saline-alkali environment, so that the transgenic plant shows stronger antioxidant capacity, higher photosynthetic efficiency and more stable membrane system under the saline-alkali stress, thereby guaranteeing normal development and quality improvement of fruits, and reducing growth inhibition and yield loss caused by stress. The application provides a reference for improvement of stress resistance of fruit trees, and has a wide agricultural application prospect.

The invention provides an A type 12, 13-epoxy trichothecene compound or a pharmaceutically acceptable salt thereof, and a compound with a chemical formula (I), and the compound has growth inhibition activity on A549, NCI-H1650, NCI-H1944 lung cancer cells and BEAS-2B normal lung cells, and can provide a lead compound for developing lung tumor treatment drugs.

The invention discloses a cyclic iridium-ester tin imidazo phenanthroline complex with an aggregation-induced emission (AIE) characteristic as well as a preparation method and an anti-cancer application of the cyclic iridium-ester tin imidazo phenanthroline complex. The structural formula is as shown in formula (I), R1 is phenyl or 3-pyridyl, and R2 is hydrogen or fluorine. By testing the growth inhibition rate of the target compound on human alveolar basal epithelial cancer cells (A549) and comparing, the target compound shows potential A549 cell proliferation inhibition activity. In addition, the target complex shows a unique AIE luminescence characteristic, and is accumulated in lysosome of A549 cells, resulting in lysosome damage and apoptosis.

Provided are a polypeptide-coupled drug and an anti-tumour use thereof. The polypeptide-coupled drug is a compound of formula (I) or a pharmaceutically acceptable salt thereof. In formula (I), P is a cyclic peptide containing 4-9 amino acids, L is a linking group, and D is selected from topoisomerase I inhibitors; the polypeptide-coupled drug has better tumour growth inhibitory activity. P-L-D (I)

Novel compositions are described, each comprising at least one active constituent that has a 12-carbon atom backbone as a 12-carbon chain base structure having at least a sub-terminal nitrogen and no terminal amino group. One of the active constituents is Boc-12-Ado-OH (BOA) and / or N,N-dimethyldodecylamine N-oxide (LO). Novel particle or liposomal compositions comprising at least BOA and / or LO may be prepared with an emulsifier and / or lipid and optionally a biopolymer, and may be formulated into pharmaceutically acceptable formulations and / or medicaments with or without one or more pharmaceutically acceptable carriers or excipients for utilization as an antimicrobial against one or more microbial pathogens. The novel antimicrobial compositions have broad-spectrum growth inhibitory or other destructive activity against microbial pathogens, biofilms produced by one or more pathogens, and biofilm infections.

The invention relates to a cultivation method of halotolerant bacteria and application of the halotolerant bacteria in agriculture, the cultivation method comprises the following steps: S1, mixing straw, mushroom cultivation waste, livestock manure, inorganic salt and a culture medium matrix to obtain a biological waste-based culture medium; s2, respectively culturing pythium aphanidermatum and bacillus by adopting the biological waste material-based culture medium to obtain a composite strain liquid; s3, diatomite and the composite strain liquid are mixed, adsorbed and dried, and an intermediate product is obtained; s4, blending chitosan, phytanic acid and an activating agent, and reacting to obtain hydrophobic chitosan; s5, blending and stirring the intermediate product and hydrophobic chitosan to obtain the halotolerant bacteria. The hydrophobic chitosan is beneficial to stable storage of the halotolerant bacteria in a high-humidity environment, and reduces adverse effects such as growth inhibition among different halotolerant bacteria, so that the different halotolerant bacteria can achieve a synergistic effect, and the efficacy of the fertilizer is finally improved.

The invention discloses application of a traditional Chinese medicine composition in preparation of a medicine for preventing and treating bacterial liver injury of chicks. The traditional Chinese medicine composition is prepared from herba artemisiae scopariae and radix scutellariae. For bacterial infection and immunological liver injury triggered by endotoxin, through compatibility and synergy of'one clearing and one benefiting 'of two medicines, pathogen stimulation and immune overresponse links are intervened at the same time, and growth inhibition caused by liver injury is blocked. The traditional Chinese medicine composition is extracted and prepared into oral liquid, powder and other dosage forms, and then is applied to chicks at the age of 6-12 days. Experiments prove that the traditional Chinese medicine composition can significantly reduce the activity of liver injury markers such as serum ALT and AST, alleviate liver inflammatory infiltration and necrosis, can reverse the decrease of average daily gain caused by liver injury, synchronously realizes liver injury repair and growth performance guarantee, adapts to resistance reduction / forbidden resistance trends, and has a broad application prospect. And a safe and effective solution is provided for preventing and treating bacterial liver injury in the early brooding stage of broilers.

The application discloses methyl phenolic compounds, a preparation method and application thereof. Six new methyl phenolic compounds are separated from the skin of eleocharis tuberosa, and the compounds all show good inhibitory activity on alpha-glucosidase, and are stronger than the clinical first-line drug acarbose. The inhibitory activity of compound 3 is the best, enzyme kinetics research shows that the compound 3 plays a role as a reversible mixed inhibitor, ADME predicts that the compound 3 has good drug properties, including high gastrointestinal absorption and no CYP450 enzyme inhibition. The cytotoxicity experiment shows that the six compounds have no obvious growth inhibition on human embryonic kidney cells HEK293 at a concentration of 100 muM, and have good safety. The compounds can be combined with a pharmaceutically acceptable carrier to form a pharmaceutical composition, and are used for preparing a health care product for reducing blood sugar or a drug for treating diabetes. The preparation method is simple, easy to operate, suitable for industrial production, and is helpful to the high-value reuse of the skin of eleocharis tuberosa.

The invention discloses a method for identifying nitrogen absorption capacity of wheat in a seedling stage based on potassium chlorate culture in combination with field experiments. The method comprises the following steps: (1) screening potassium chlorate with proper concentration; (2) after the seeds of the different wheat varieties germinate, transplanting the seeds into a culture medium containing potassium chlorate with a proper concentration, culturing the seeds to a one-leaf and one-core period, and calculating the growth inhibition ratio of the different wheat varieties so as to evaluate the sensitivity of the different wheat varieties to the potassium chlorate sensitivity; (3) field trials are adopted at the same time, crops of different wheat varieties are planted randomly, the trials take nitrogen fertilizer as main treatment, and three nitrogen element levels are designed in total and are respectively a group without nitrogen fertilizer application, a group with normal nitrogen application and a group with high nitrogen; and in the test process, observing and recording agronomic characters of each test material. According to the method, potassium chlorate screening in the seedling stage is combined with field agronomic character single plant grain weight growth rate comprehensive evaluation, and the efficient nitrogen absorption germplasm can be accurately identified.

This invention belongs to the field of plant genetic engineering and plant stress resistance biotechnology, and discloses... ZmCYP81Q32 Application of genes in improving nicosulfuron resistance in plants. This invention provides... ZmCYP81Q32 The nucleotide sequence of the gene, as shown in SEQ ID NO:1, encodes a cytochrome P450 enzyme that can directly bind to sulfonylurea herbicides and promote their hydroxylation metabolism. This reduces the accumulation of the parent herbicide in the plant, alleviates herbicide-induced growth inhibition, leaf chlorosis, and oxidative damage, and significantly improves plant tolerance to sulfonylurea herbicides, especially enhancing plant resistance to nicosulfuron. This invention can be widely used for crop herbicide resistance improvement, herbicide-tolerant germplasm creation, and related molecular breeding.

The invention discloses a windmill-shaped alpha-Fe2O3 / Ag nano hybrid material and a preparation method and application thereof in relieving plant salt stress, and the method comprises the following steps: mixing fusiform alpha-Fe2O3 prepared by a forced hydrolysis method with silver acetate, then carrying out ball milling treatment, and then carrying out a high-temperature calcination process to obtain the windmill-shaped alpha-Fe2O3 / Ag nano hybrid material. And the windmill-shaped alpha-Fe2O3 / Ag hybrid structure formed by assembling the fusiform alpha-Fe2O3 and the Ag nanoparticles is obtained. The nano hybrid material has high specific surface area, excellent dispersity and remarkable leaf surface adhesion performance, when the nano hybrid material is applied to plant cultivation, the material can effectively relieve growth inhibition caused by salt stress, can regulate and control plant hormone signals, terpene and carbohydrate metabolism, sulfur metabolism, protein homeostasis and the like, realizes multi-channel comprehensive regulation, and has a wide application prospect. Therefore, the plant stress resistance and quality are improved.

The present invention addresses the problem of providing a highly safe and continuously ingestible agent for: preventing or ameliorating diseases attributed to microorganisms belonging to the family Peptostreptococcaceae by inhibiting the growth of these microorganisms in the intestine; or for preventing or ameliorating diseases potentially preventable or ameliorable by a substance produced from microorganisms belonging to the genus Slackia by promoting the growth of these microorganisms in the intestine.　The problem of the present invention has been solved by a growth inhibitor of microorganisms belonging to the family Peptostreptococcaceae or a growth promotor of microorganisms belonging to the genus Slackia, each containing a galactooligosaccharide as an active ingredient.

The invention discloses a preparation method for directionally inducing different lignocellulases through traditional Chinese medicine residues and application of the lignocellulases in degradation and conversion of traditional Chinese medicine compound residues, and relates to the field of biotechnology and fermentation engineering. Due to the fact that residual medicinal components in traditional Chinese medicine residues and lignocellulose form a tight resistance barrier, common strains are often subjected to a growth inhibition effect, and enzyme activity is reduced. The strain provided by the invention can be used for effectively fermenting multiple types and multiple parts of traditional Chinese medicine residues, and the traditional Chinese medicine residues of different medicinal parts are used as carbon sources to obtain an efficient cellulase system and an auxiliary enzyme system under the action of penicillium oxalicum Z2. By utilizing the structural characteristics of the traditional Chinese medicine residues at different medicinal parts, the lignocellulase with high pertinence and high efficiency is obtained. The targeted lignocellulase prepared by the method shows remarkable advantages in degradation and conversion of the traditional Chinese medicine compound dregs, and a new way is opened up for resource utilization of the traditional Chinese medicine dregs through formula optimization and enzyme synergistic degradation in combination with the structural characteristics of the compound dregs.

The invention discloses a programmed function switching-based calcium sulfate whisker length-diameter ratio accurate regulation and control method, and belongs to the technical field of inorganic functional crystal material preparation. The traditional static regulation and control limitation is broken through, and a new mechanism of programmed adsorption-desorption function switching is created for the first time. The preparation method comprises the following steps: constructing an initial growth inhibition template by using amino trimethylene phosphonic acid (ATMP) as a crystal face adsorbent; in different stages of crystal growth, desorption agents with complementary functions are introduced in stages: sodium citrate is introduced in a competitive growth period, and radial growth inhibition is partially relieved; polyvinyl alcohol (PVA) is introduced in the length-diameter ratio curing period, and material transmission at the axial growth end is specifically promoted. The function switching process is precisely coupled with a gradient temperature change process, and sequential dynamic reconstruction of a crystal growth interface is realized. According to the invention, the length-diameter ratio of the calcium sulfate whisker is regulated and controlled in an ultra-wide range of 50-500, the product uniformity is improved, and a raw material with better performance is provided for the fields of high-end composite materials and the like.

This invention discloses a kit for constructing an iron-dependent growth environment for Acinetobacter baumannii and its application method, belonging to the field of biotechnology. The kit is specifically designed for Acinetobacter baumannii, and its core components include a 50 mmol / L sterile stock solution tube of 2,2′-bipyridine, a 2000 μmol / L sterile stock solution tube of FeCl3, and a user manual. It does not include general-purpose LB medium, thus avoiding the problem of high-temperature and high-pressure deterioration of core reagents and highlighting the innovative combination of dedicated reagents. This invention also provides the application method of the kit. By adding the kit stock solution to the autoclaved and cooled LB medium in a specific ratio, standardized growth environments with normal iron, iron deficiency, and iron enrichment can be rapidly constructed. Dynamic monitoring and verification of the reversibility of growth inhibition-recovery ensure that the iron environment is precise and controllable. The kit is easy to operate, has good reproducibility, and can achieve standardized construction of the iron environment for Acinetobacter baumannii. It is suitable for iron metabolism-related research such as functional analysis of iron uptake-related proteins, development of iron deprivation antibacterial strategies, and screening of iron-deficient strains.

The application belongs to the technical field of natural compound application, and particularly relates to application of globulol in preparation of lysine-deficient Staphylococcus aureus. The application provides, for the first time, application of globulol in preparation of lysine-deficient Staphylococcus aureus. Globulol in the application is a natural monomer component in eucalyptus, and is a natural sesquiterpene compound. After endogenous lysine is consumed by pre-treatment according to the application, S. aureus Globulol has obvious growth inhibition effect on Staphylococcus aureus in a lysine-deficient culture system, and the growth inhibition phenomenon is obviously recovered after exogenous lysine is supplemented under the same control.

The present invention relates to a tobacco black shank pathogen selective culture medium and a preparation method and application thereof. The present invention uses different types and different concentration gradients of poisonous oat culture medium plates to respectively measure the growth inhibition rates of Fusarium oxysporum, Fusarium solani and Phytophthora nicotianae, the main pathogens of root rot, on the plates, and finds that tebuconazole and imazalil have the best inhibition effect on tobacco root rot and have no effect on the growth of tobacco black shank pathogen. Therefore, the above culture medium can be used to screen and separate tobacco black shank pathogen strains. The tobacco black shank pathogen selective culture medium and screening and separation method provided by the present invention can be used to separate pathogens from tobacco black shank pathogenic plants, especially for plants with tobacco root rot complex infection, and has the characteristics of excellent separation effect, simple operation, time saving and labor saving, etc., and has broad application prospects.