147 results about "Growth inhibitory" patented technology

The invention provides a bacillus velezensis strain and application thereof. The name of the bacillus velezensis is BJ-1141, and the bacillus velezensis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.32094. The strain has broad-spectrum antibacterial activity, and has a good inhibition effect on 24 plant pathogenic fungi such as rice pathogenic bacteria, wheat pathogenic bacteria, corn pathogenic bacteria, grape pathogenic bacteria, cherry pathogenic bacteria, solanaceous crop pathogenic bacteria and the like. Wherein the growth inhibition effect on sclerotinia sclerotiorum hyphae causing eggplant sclerotinia rot is optimal, and the inhibition rate can reach 95.57%. The BJ-1141 bacterial liquid has an obvious growth promoting effect on the growth of plants such as tomatoes and lettuce. After the BJ-1141 bacterial liquid is used for irrigating roots of potted tomatoes, the tomato fusarium wilt caused by tomato fusarium oxysporum can be effectively controlled, the BJ-1141 bacterial liquid has a good prevention and treatment effect, the prevention and treatment effect can reach 85.93%, and the prevention and treatment effect is obviously different from that of a control group.

The invention discloses lactobacillus mucilaginosus and application thereof in fermentation of sporoderm-broken ganoderma lucidum spore powder. The preservation number of the lactobacillus mucilaginosus is CGMCC (China General Microbiological Culture Collection Center) No.31453. The strain is separated and screened from vaginal secretions of healthy women at the childbearing age, is not hemolyzed, is sensitive to various common antibiotics and has strong lactic acid production capacity, and fermented supernate of the strain has strong growth inhibition capacity on escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, salmonella typhimurium, shigella dysenteriae and gardnerella vaginalis and can be used for producing lactic acid. The compound has strong adhesion ability, growth inhibition ability and apoptosis promoting effect on human cervical cancer cells. When the strain is used for fermenting wall-broken ganoderma lucidum spore powder, the content of bioactive substances such as amino acids and derivatives thereof, fatty acids and steroids in fermentation liquor can be greatly increased, the strain has an obvious anti-inflammatory effect on DSS-induced mouse colitis, and the inflammatory factor level is remarkably reduced. The strain not only has significant application value in functional food and biological agents, but also opens up a new way for further development of ganoderma spores.

The invention belongs to the technical field of microorganisms, and discloses a Lactobacillus paracasei B20 for inhibiting melanin from a plateau area and application of the Lactobacillus paracasei B20, the Lactobacillus paracasei B20 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.32434, and the preservation date is October 31, 2024. Metabolite of the lactobacillus paracasei B20 comprises phenylalanine, p-hydroxycinnamic acid and the like, has strong tyrosinase inhibition effect and anti-ultraviolet ability, and can significantly reduce generation of melanin in skin. In addition, the lactobacillus paracasei B20 also has relatively strong oxidation resistance, has good tolerance in artificial gastric juice and artificial intestinal juice, and can smoothly reach the intestinal tract of a human body. In addition, the lactobacillus paracasei B20 has a relatively obvious growth inhibition effect on skin conditioned pathogens such as staphylococcus aureus and enterococcus faecalis.

The invention discloses application of biological silver nanoparticles synthesized based on bacillus velezensis in prevention and treatment of tomato gray mold, and relates to the technical field of prevention and treatment of tomato gray mold. Researches find that the biological silver nanoparticles Bio-AgNPs have a relatively strong inhibition effect on the growth of botrytis cinerea hyphae, and when the concentration of the biological silver nanoparticles Bio-AgNPs is 100 mu g / mL, the growth inhibition rate of the botrytis cinerea hyphae is 100%. The Bio-AgNPs shows a good prevention and treatment effect on the tomato gray mold, after 40 [mu] g / mL of Bio-AgNPs is used for treating tomato fruits and leaves, the scab of the tomato fruits is obviously reduced by 52.21%, and the prevention and treatment effect on the tomato leaf gray mold is 40.19%.

The invention discloses a method for predicting a composite biological effect of a carbon nanomaterial. The method comprises the following steps: measuring the growth inhibition toxicity of different types of carbon-based nanomaterials to scenedesmus obliquus in the presence of singly; the growth inhibition toxicity is calculated by taking the effect multiple change generated by a treatment group compared with a control group as a toxicity index; based on the obtained growth inhibition toxicity data, constructing an SEML prediction model through a support vector machine regression algorithm or an artificial neural network regression algorithm; and predicting the composite biological effect of the carbon nanomaterial according to the SEML prediction model. According to the method, the effect multiple change FC is adopted as a toxicity index, biotoxicity evaluation is more accurate, meanwhile, the prediction model is directly constructed based on growth inhibition toxicity data, material characteristic parameters of the carbon-based nano material do not need to be measured, and the modeling process is more convenient.

The invention discloses a method for effectively relieving soybean antigen protein Glycinin in fish feed and application of the method. The invention discloses application of N-acetylcysteine as a functional feed additive capable of relieving carp enteritis induced by soybean antigen protein Glycinin. The feed disclosed by the invention comprises a soybean antigen protein Glycinin and N-acetylcysteine (NAC). According to the present invention, 1.5-6.0 g / kg of NAC is added to a feed containing 80 g / kg of Glycinin, such that the fish growth, the feed utilization rate, the digestion function, the protein metabolism function, the immune function, the oxidation resistance and the intestinal morphology can be significantly improved, and the soybean antigen protein Glycinin-induced growth inhibition and the intestinal injury can be effectively relieved; the functional fish feed additive provided by the invention is relatively low in cost and easy to popularize, and is beneficial to development of novel green antibiotic-replacing additives, improvement of the utilization rate of soybean protein sources, reduction of feed cost and promotion of healthy culture of fishes.

Using chemical genetic screening, we discovered a small molecule Cellulosin (aka endosidin20 or ES20) that causes cell swollen and inhibits plant growth, but does not disrupt global vesicle trafficking. By doing mutant screening, we obtained multiple alleles of Arabidopsis thaliana that are resistant to Cellulosin inhibition in growth. Those mutated amino acid residues are conserved across plant species. Cellulosin targets a group of cellulose synthases (CesAs) of Arabidopsis thaliana by binding to a conserved domain essential for the catalytic activity of CesA. Cellulosin may target and inhibit all subtypes of CesAs in plants. The present invention relates to Cellulosin, a cellulose synthase inhibitor, its analogs or derivatives as a broad-spectrum herbicide. The mutated genes, their protein products and a cell or a plant having those mutated genes or expressing those protein products are within the scope of this disclosure.

The invention is suitable for the technical field of agricultural production, and provides a preparation method and application of a 2, 4-di-tert-butylphenol-betaine eutectic. Through a crystal engineering technology and a co-crystallization strategy, the thermal stability and solubility of the 2, 4-di-tert-butylphenol are remarkably improved, and the volatility of the 2, 4-di-tert-butylphenol is reduced. Compared with 2, 4-di-tert-butylphenol, the 2, 4-di-tert-butylphenol-betaine eutectic synthesized by the invention has the advantages that the growth inhibition effect on farmland weeds such as ryegrass, green bristlegrass, water barnyard grass, purslane, amaranthus retroflexus and sticktight is remarkably improved, and the great potential in the aspect of long-acting inhibition of agricultural weeds is shown. In addition, the 2, 4-di-tert-butylphenol and the betaine are natural source components, and are environment-friendly and low in cost. Meanwhile, by means of a grinding-induced self-assembly method, the dosage of an organic solvent is effectively reduced, the production cost is further reduced, and therefore the feasibility of developing the material into a commercial product is improved.

The present invention belongs to the field of plant growth regulators, and specifically relates to a fruit coloring composition, a fruit coloring functional fertilizer and their applications. The fruit coloring composition comprises 1-aminocyclopropane carboxylic acid and a protective auxiliary agent, and the mass ratio of 1-aminocyclopropane carboxylic acid to the protective auxiliary agent is 1:0.1 to 200; the protective auxiliary agent is selected from 5-aminolevulinic acid, diethyl aminoethyl hexanoate or choline chloride. The present invention first discloses the use of protective auxiliary agents such as 5-aminolevulinic acid, which can effectively improve the adverse effects when ACC is applied alone, such as high dosage for coloring, easy to cause negative phenomena such as plant defoliation, fruit cracking, fruit drop and quality decline. When the effective application dosage is only 0.05 to 2.5 ppm, excellent coloring effect can be achieved. At the same time, the safety of exogenous application of ACC is effectively improved, and the planting effect of fruit and vegetable crops can be improved in aspects such as preventing fruit drop, increasing fruit sweetness, reducing phytotoxicity, and relieving the growth inhibition of stems, leaves and fruits.

The invention relates to the technical field of cadmium pollution control. The invention provides a method for relieving soybean cadmium stress by using 2, 4-epibrassinolide and a method for comparing the relieving effect of 2, 4-epibrassinolide on soybean cadmium stress, and the method comprises the following steps: spraying 2, 4-epibrassinolide with the concentration of 0.1-1.0 [mu] mol / L on soybean leaves, and continuously spraying for 2-5 days. Soybean varieties with large cadmium sensitivity difference are adopted for water culture test, cadmium treatment is performed on seedlings of the two varieties, 2, 4-epibrassinolide pretreatment is performed at the same time, the influence of 2, 4-epibrassinolide pretreatment on growth inhibition and physiological characteristics of cadmium stress of the soybean seedlings is studied, and the cadmium stress resistance of the soybean seedlings is improved. Potential effects of response and resistance mechanisms of plants under cadmium stress in Cd tolerance are discussed, and a theoretical basis is provided for cultivation of cadmium-tolerant and low-cadmium-accumulation soybean varieties.

The invention discloses a nano-silver algae growth inhibition level prediction method based on a random forest, and the method comprises the following steps: collecting the physical property, chemical property, experimental method and environmental condition data of nano-silver, and obtaining a data set comprising a descriptor of the nano-silver and algae growth inhibition data; aiming at missing values existing in the data set, preprocessing the data, and dividing the data set into a training set and a verification set; constructing a nano-silver algae growth inhibition random forest prediction model, and training the random forest prediction model by using the training set data until the random forest prediction model is stably fitted; and inputting verification set data into the trained random forest prediction model, and outputting a nano-silver algae growth inhibition level prediction value by the random forest prediction model for evaluating the nano-silver ecological risk. The algae growth inhibition level of the nano-silver is rapidly predicted, the method is simple and rapid, the cost is low, and manpower, material resources and financial resources required by experimental determination are saved.

One embodiment of the present invention provides a psicose epimerase expression cassette comprising a polynucleotide encoding a psicose epimerase and a promoter operably linked thereto and modulating the expression of the psicose epimerase in a Corynebacterium strain. The promoter consists of a base sequence as shown in a sequence 1, a base sequence as shown in a sequence 9, a base sequence as shown in a sequence 10 or a base sequence as shown in a sequence 11. The psicose epimerase expression cassette disclosed by the embodiment of the invention is almost not started in a normal environment for culturing a recombinant corynebacterium strain, and does not induce growth inhibition of the recombinant corynebacterium strain. Therefore, the recombinant corynebacterium strain transformed by the psicose epimerase expression cassette of one embodiment of the present invention can normally grow and proliferate to the logarithmic phase, and stably and highly express psicose epimerase after the logarithmic phase.

The invention provides a lactobacillus plantarum 18 which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No.31277. The lactobacillus plantarum 18 has the advantages that the lactobacillus plantarum 18 is prepared from lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum and lactobacillus plantarum; the plant lactobacillus 18 can inhibit growth of propionibacterium acnes, inhibit expression of virulence factors (biological membranes, lipase and the like) of the propionibacterium acnes, relieve inflammation, inhibit autophagy of cells, scavenge free radicals and inhibit activity of tyrosinase, so that the plant lactobacillus 18 has the effects of resisting acnes, dermatitis and oxidation and whitening skin. The plant lactobacillus 18 or the metabolite of the plant lactobacillus 18 can be used for preparing products with the effects or effects.

Novel compositions are described, each comprising at least one active constituent that has a 12-carbon atom backbone as a 12-carbon chain base structure having at least a sub-terminal nitrogen and no terminal amino group. One of the active constituents is Boc-12-Ado-OH (BOA) and / or N,N-dimethyldodecylamine N-oxide (LO). Novel particle or liposomal compositions comprising at least BOA and / or LO may be prepared with an emulsifier and / or lipid and optionally a biopolymer, and may be formulated into pharmaceutically acceptable formulations and / or medicaments with or without one or more pharmaceutically acceptable carriers or excipients for utilization as an antimicrobial against one or more microbial pathogens. The novel antimicrobial compositions have broad-spectrum growth inhibitory or other destructive activity against microbial pathogens, biofilms produced by one or more pathogens, and biofilm infections.

The present invention relates to a composition for inhibiting hair loss, promoting new hair growth, or promoting hair growth, comprising a tubulin inhibitor as an active ingredient. Also, the present invention relates to a pharmaceutical composition for inhibiting hair loss, promoting new hair growth, or promoting hair growth, comprising a tubulin inhibitor as an active ingredient. Also, the present invention relates to a quasi-drug composition for inhibiting hair loss, promoting new hair growth, or promoting hair growth, comprising a tubulin inhibitor as an active ingredient. Also, the present invention relates to a cosmetic composition for inhibiting hair loss, promoting new hair growth, promoting hair growth, and alleviating symptoms of hair loss, comprising a tubulin inhibitor as an active ingredient. Also, the present invention relates to a hair or scalp product comprising a tubulin inhibitor as an active ingredient. When the tubulin inhibitor of the present invention was applied to an animal model, the animal model was confirmed to exhibit significantly increased new hair growth, hair loss inhibition, and hair growth promotion effects, compared to a control. Therefore, the tubulin inhibitor of the present invention can be effectively used as a medicine or cosmetic product for hair loss or hair growth.

The present invention provides the use of chrysin to enhance the sensitization of VS4718 or ponatinib in the treatment of esophageal squamous cell carcinoma. The present invention finds that chrysin can enhance the growth inhibition and invasion inhibition of esophageal squamous cell carcinoma cells mediated by VS4718 or ponatinib, and can effectively enhance the tumor metabolism inhibition mediated by VS4718 or ponatinib. Chrysin is used in combination with VS4718 or ponatinib to produce a synergistic anti-cancer effect.

The invention discloses bacillus amyloliquefaciens capable of resisting plant pathogenic bacteria and application of the bacillus amyloliquefaciens, and belongs to the technical field of microorganisms, the bacillus amyloliquefaciens is classified and named as bacillus amyloliquefaciens KT-7, the bacillus amyloliquefaciens KT-7 is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on June 9, 2020, and the address is No.66, Courtyard No.100, Xianlie Middle Road, Guangzhou City, Guangdong Province; the preservation number of the strain is GDMCC No: 61051. The bacillus amyloliquefaciens provided by the invention has a growth inhibition effect on plant pathogenic bacteria, and particularly has a good control effect on bacterial diseases such as citrus canker and tomato bacterial wilt.

The invention discloses application of perillyl alcohol in preparation of a medicament for inhibiting growth of fusarium graminearum and the medicament containing the perillyl alcohol. The application mechanism is that perillyl alcohol destroys the mycelium cell membrane integrity of fusarium graminearum. According to the application disclosed by the invention, the growth inhibition effect of the perillyl alcohol on the fusarium graminearum which is a corn pathogenic bacterium is found for the first time, a series of experiments prove that the perillyl alcohol inhibits the growth of the fusarium graminearum by destroying the main component ergosterol of a fungal cell wall, and the inhibition effect is superior to that of a common bactericide carbendazim; and a foundation is laid for the development of pesticides for resisting pathogenic bacteria of corn.

This invention discloses a stress-resistant and yield-increasing plant growth regulator, its preparation method, and its application, belonging to the field of plant growth regulators. The stress-resistant and yield-increasing plant growth regulator of this invention comprises the following raw materials: *Suaeda salsa* extract, a stress-resistant biostimulant, propionyl brassinolide, styracil, polyaspartic acid, γ-aminobutyric acid, emulsifier, penetrant, co-emulsifier, pH buffer, and deionized water; wherein the stress-resistant biostimulant is perilla lactone. This invention significantly improves the tolerance of wheat to salt-alkali stress by strengthening the antioxidant system and osmotic regulation capacity, ensuring its basic survival and normal physiological functions; and effectively reverses stress-induced growth inhibition through mechanisms such as promoting root development, enhancing photosynthesis, and driving product translocation to grains, thereby achieving a significant yield increase under adverse conditions.

The invention discloses application of an aromatic hydrocarbon receptor stimulant in preparation of a medicine for treating diarrhea of weaned piglets and / or inhibiting inflammation, and belongs to the field of biological medicine. According to the present invention, with the PAS-B structural domain of the AHR as the target, the virtual screening technology, the target verification test and the weaned piglet diarrhea pharmacodynamic evaluation test are performed to determine the arctigenin as the AHR strong agonist and the anti-inflammatory drug, and the arctigenin has the good treatment effect on the weaned piglet diarrhea. Experimental results show that 10 mg / kg of arctigenin can significantly relieve diarrhea and hemafecia symptoms of weaned piglets, reduce the content of inflammatory factors in serum and intestinal tissues and improve the intestinal structure of weaned piglets, has no significant cytotoxicity, hepatorenal toxicity and intestinal epithelial cell growth inhibition effect, and can be used for preparing a feed additive for weaned piglets. The compound can be used for preparing medicines for treating diarrhea of weaned piglets. The invention provides a new raw material medicine for treating diarrhea of weaned piglets, and has great economic value and wide application prospect.

The application discloses application of MdERF77 gene in plant resistance to saline-alkali stress, and belongs to the technical field of biotechnology. The application overexpresses the MdERF77 gene in an apple plant through transgenic technology, significantly enhances the tolerance of the plant to saline-alkali stress, and has outstanding technical effects. The method can effectively improve the growth adaptability of the apple in a saline-alkali environment, so that the transgenic plant shows stronger antioxidant capacity, higher photosynthetic efficiency and more stable membrane system under the saline-alkali stress, thereby guaranteeing normal development and quality improvement of fruits, and reducing growth inhibition and yield loss caused by stress. The application provides a reference for improvement of stress resistance of fruit trees, and has a wide agricultural application prospect.

The invention provides an A type 12, 13-epoxy trichothecene compound or a pharmaceutically acceptable salt thereof, and a compound with a chemical formula (I), and the compound has growth inhibition activity on A549, NCI-H1650, NCI-H1944 lung cancer cells and BEAS-2B normal lung cells, and can provide a lead compound for developing lung tumor treatment drugs.

The invention belongs to the technical field of biological energy and environmental engineering, and particularly discloses a method for treating a chlorella circulating culture solution through Fenton oxidative coupling activated carbon adsorption to solve the growth inhibition phenomenon caused by extracellular polymeric substance accumulation in the microalgae circulating culture process so as to achieve cyclic utilization of the chlorella culture solution and application of the chlorella culture solution. By means of Fenton oxidative coupling activated carbon adsorption, chlorella extracellular polymeric substances (EPS) can be effectively degraded and oxidized and decomposed into small-molecule organic substances, the growth inhibition effect of the small-molecule organic substances on chlorella is relieved, an organic carbon source can be provided for growth and metabolism of chlorella, and growth of chlorella is remarkably promoted. According to the method, the chlorella circulating wastewater is treated and recycled through Fenton oxidative coupling activated carbon adsorption, so that the yield of chlorella biomass can be effectively increased, and the production and culture cost is reduced.

A compound represented by Chemical Formula 1 or 2 of the present invention, or a pharmaceutically acceptable salt thereof can be used favorably for preventing or treating cancer or tumors.

The invention discloses a cyclic iridium-ester tin imidazo phenanthroline complex with an aggregation-induced emission (AIE) characteristic as well as a preparation method and an anti-cancer application of the cyclic iridium-ester tin imidazo phenanthroline complex. The structural formula is as shown in formula (I), R1 is phenyl or 3-pyridyl, and R2 is hydrogen or fluorine. By testing the growth inhibition rate of the target compound on human alveolar basal epithelial cancer cells (A549) and comparing, the target compound shows potential A549 cell proliferation inhibition activity. In addition, the target complex shows a unique AIE luminescence characteristic, and is accumulated in lysosome of A549 cells, resulting in lysosome damage and apoptosis.

The present invention is applicable to the field of agricultural production technology, and provides a preparation method and application of a 2,4-di-tert-butylphenol-betaine eutectic. The present invention adopts a eutectic strategy through crystal engineering technology, significantly improves the thermal stability and solubility of 2,4-di-tert-butylphenol, and reduces its volatility. The 2,4-di-tert-butylphenol-betaine eutectic synthesized by the present invention, compared with 2,4-di-tert-butylphenol, significantly improves the growth inhibition of farmland weeds such as ryegrass, foxtail grass, water barnyard grass, purslane, Amaranthus retroflexus and Bidens pilosa, showing great potential in the long-term inhibition of agricultural weeds. In addition, 2,4-di-tert-butylphenol and betaine are both natural source ingredients, environmentally friendly and low cost. At the same time, the present invention effectively reduces the amount of organic solvent used by grinding-induced self-assembly method, further reduces production costs, and thus improves the feasibility of developing it into a commercial product.

Provided are a polypeptide-coupled drug and an anti-tumour use thereof. The polypeptide-coupled drug is a compound of formula (I) or a pharmaceutically acceptable salt thereof. In formula (I), P is a cyclic peptide containing 4-9 amino acids, L is a linking group, and D is selected from topoisomerase I inhibitors; the polypeptide-coupled drug has better tumour growth inhibitory activity. P-L-D (I)

Novel compositions are described, each comprising at least one active constituent that has a 12-carbon atom backbone as a 12-carbon chain base structure having at least a sub-terminal nitrogen and no terminal amino group. One of the active constituents is Boc-12-Ado-OH (BOA) and / or N,N-dimethyldodecylamine N-oxide (LO). Novel particle or liposomal compositions comprising at least BOA and / or LO may be prepared with an emulsifier and / or lipid and optionally a biopolymer, and may be formulated into pharmaceutically acceptable formulations and / or medicaments with or without one or more pharmaceutically acceptable carriers or excipients for utilization as an antimicrobial against one or more microbial pathogens. The novel antimicrobial compositions have broad-spectrum growth inhibitory or other destructive activity against microbial pathogens, biofilms produced by one or more pathogens, and biofilm infections.

The invention provides a preparation method of folic acid modified SN38 derivative lipidosome and application of the folic acid modified SN38 derivative lipidosome in tumor treatment. A blank folic acid liposome containing glutathione (GSH) is prepared through a film ultrasonic method, and an SN38 derivative solution and the blank folic acid liposome are mixed and incubated to obtain the SN38 derivative folic acid liposome. The SN38 folic acid lipidosome prepared by the invention is of a spherical vesicle structure, and is regular in form and free of rupture; the average particle size is 118 nm, and the Zeta potential is-25 mV; and good stability and obvious slow release performance are realized. The SN38 folic acid lipid disclosed by the invention shows enhanced cytotoxicity and cellular uptake, which proves that the SN38 folic acid lipid has enhanced tumor targeting ability. Both the SN38 folic acid liposome and the SN38-Atba folic acid liposome disclosed by the invention can generate a remarkable growth inhibition effect on tumors, and the treatment effect of the SN38 folic acid liposome and the SN38-Atba folic acid liposome is obviously superior to that of the SN38 liposome and a first-line chemotherapeutic drug irinotecan clinically used for treating the tumors. Therefore, the invention provides a new treatment strategy for tumors, and has very important clinical significance and social significance.