18 results about "Candida parapsilosis" patented technology

The invention discloses a method for efficient conversion and utilization of mogroside. According to the method, mogroside V is converted through fermentation of candida parapsilosis L7, and siamenoside I is directionally synthesized. According to the invention, Candida parapsiloides L7 is utilized for fermentation conversion, and beta-1, 6-glucosidic bonds in glucose branched chains connected with C3-site hydroxyl of a mogroside V structure parent nucleus can be directionally hydrolyzed with high regioselectivity (the beta-1, 6-glucosidic bonds on glucose branched chains connected with C24-site hydroxyl are hardly hydrolyzed), so that one molecule of glucose is removed, and siamenoside I is obtained. The siamenoside I provided by the invention can be efficiently prepared, and the maximum molar yield of the siamenoside I can reach 87.40%. The siamenoside I has more obvious selective hydrolysis characteristic, higher space time yield of the siamenoside I, no cytotoxicity of the siamenoside I and better anti-inflammatory and antioxidant activity, provides a new drug choice for diseases such as inflammation and the like, and provides technical support for development of high-valued products of mogroside.

The invention provides an efficient fermentation production method and application of acid type sophorolipid, and the production method comprises the following steps: (1) in a growth stage, adding a composite nitrogen source and growth factors into a basic culture medium to promote the growth of zymophyte and realize high-density fermentation, the zymophyte being candida utilis subjected to genetic engineering modification; (2) in the product synthesis stage, mixed syrup and vegetable oil are used as carbon sources, and the concentration of glucose in a fermentation tank is maintained at 1-5g / L and the concentration of residual oil is maintained at 1-5g / L by regulating and controlling the material supplementing rate. The fermentation production method provided by the invention solves the problems of slow thallus growth, long fermentation period, low production intensity, uncontrollable product composition, more byproducts, peculiar smell of fermentation tail gas and the like in the existing sophorolipid production process, and has industrial application value.

The invention provides a primer probe set for detecting candida bloodstream infection, a kit and application, and belongs to the technical field of microbiological detection. The primer probe group for detecting candida bloodstream infection comprises a primer probe group 1 and / or a primer probe group 2, the primer probe group 1 is one or more of a primer probe for detecting candida albicans, a primer probe for detecting candida tropicalis and a primer probe for detecting candida glabrata; and the primer probe group 2 is one or two of a primer probe for detecting candida krusei and a primer probe for detecting candida parapsilosis. The primer probe group 1 disclosed by the invention can be used for simultaneously and rapidly detecting candida albicans, candida tropicalis and candida glabrata; the primer probe group 2 can be used for simultaneously and rapidly detecting the candida krusei and the candida parapsilosis, and has the characteristics of high sensitivity, high detection specificity, simplicity and convenience in operation, short detection time and the like.

The application belongs to the field of microorganism and fermentation engineering, and provides a Candida magnoliae with high yield of 3-methylthiopropanol, and the preservation number is CGMCC NO. 23668. The Candida magnoliae can be used for producing 3-methylthiopropanol, the yield can reach 3.16 g / L, and the ethanol tolerance is high, and can be applied in the wine making, condiment and food additive industries.

The invention discloses a Candida parapsilosi GDMCC 66465 strain capable of automatically separating and preserving breeds and being used for sausage fermentation and an application method of the Candida parapsilosi GDMCC 66465 strain, and belongs to the field of food microorganisms. The bacterium is screened from Xuanwei hams, can be used for cooperatively fermenting sausages with lactobacillus plantarum, and can be used for remarkably promoting degradation of muscle protein and selective hydrolysis of triglyceride to strengthen the flavor of the hams. GC-IMS detection shows that the content of key flavor substances such as aldehydes and ketones in the finished sausage is remarkably increased, and the flavor score is increased by 30% or above compared with that of a control group. Genome sequencing analysis shows that the strain has no pathogenic risk and is a potential strain suitable for industrial production of fermented meat products.

This invention discloses the application of larvicin or its pharmaceutical salt in the preparation of antifungal drugs. The structure of larvicin is shown below. This invention discovers an effective antifungal compound with pharmaceutical potential: larvicin. The compound larvicin of this invention exhibits clear antifungal activity against pathogenic fungi such as *Candida albicans*, *Candida auris*, *Candida tropicalis*, *Candida parapsilosis*, *Candida krusei*, *Cryptococcus neoformans*, *Trichophyton mentagrophytes*, and *Trichophyton rubrum*, with a minimum inhibitory concentration (MIC). 90 The range is 2–8 μg / mL.

The present invention relates to a carbonyl reductase mutant and its application in preparing a key intermediate of diltiazem, belonging to the field of bioengineering technology. Specifically, it relates to a carbonyl reductase mutant derived from Candida parapsilosis with significantly improved specific activity, its encoding nucleic acid, a recombinant expression vector and a recombinant expression transformant containing the recombinant carbonyl reductase mutant gene, and the use of the carbonyl reductase mutant as a catalyst in preparing the key intermediate of diltiazem, 2-chloro-3-(4-methoxyphenyl)-3(S)-hydroxypropionic acid methyl ester. Compared with the parent enzyme, the carbonyl reductase mutant of the present invention catalyzes asymmetric reduction to prepare the chiral hydroxy compound 2-chloro-3(S)-hydroxy-3-phenylpropionic acid methyl ester. The chiral hydroxy compound can further generate the cardiovascular drug diltiazem chiral intermediate methyl glycidyl (2R, 3S)-p-methoxyphenyl glycidyl ester through intramolecular cyclization, which has good industrial application prospects.

The invention discloses a carbonyl reductase mutant, a coding gene and application of the carbonyl reductase mutant in synthesis of 4-tert-butylcyclohexanol, and belongs to the technical field of bioengineering. The carbonyl reductase mutant is obtained by carrying out amino acid mutation on short-chain dehydrogenase from escherichia coli K12 with an amino acid sequence shown as SEQ ID NO.1 or carrying out amino acid mutation on medium-chain dehydrogenase from candida parapsilosis with an amino acid sequence shown as SEQ ID NO.2. Compared with wild type carbonyl reductase, the carbonyl reductase mutant provided by the invention has higher enzyme activity, cis-4-tert-butyl cyclohexanol or trans-4-tert-butyl cyclohexanol can be prepared by taking 4-tert-butyl cyclohexanone as a substrate, the product yield is high, no by-product is generated, and the carbonyl reductase mutant has a very good industrial application prospect.

The invention discloses a multiple qPCR (quantitative polymerase chain reaction) detection reagent for detecting pathogens of a superficial fungal disease. The multiple qPCR detection reagent comprises specific primers and probes for detecting trichophyton rubrum, epidermophyton flocculent, microsporum canine, trichophyton beard, candida parapsilosis, candida albicans, malassezia furfur and gliocladium breviculare, wherein the specific primers and the probes are used for detecting trichophyton rubrum, epidermophyton flocculent, microsporum canine, trichophyton beard, candida parapsilosis; according to the invention, different fluorescently-labeled probes are utilized to add specific primers and probes of multiple pathogens into a reaction system to detect multiple targets at the same time, and the method has the advantages of good specificity, high sensitivity, high detection speed, capability of detecting multiple pathogens at the same time, rapidness, simplicity, convenience and economy; a convenient method is provided for rapid detection of superficial fungal disease pathogens, and the kit has important significance in epidemiological research, clinical early molecular diagnosis and the like.

The invention discloses candida parapsilosis and application thereof in extraction of pummelo peel leaf essential oil. The name of the candida parapsilosis is candida parapsilosis JY12, the preservation number of the candida parapsilosis is GDMCC NO: 64469, and the candida parapsilosis is preserved in Guangdong Microbial Culture Collection Center on March 29, 2024. The exocarpium leaf essential oil is finally prepared by taking exocarpium leaves as a raw material, fermenting the exocarpium leaves through candida parapsilosis, extracting the exocarpium leaves through a mixed organic solvent of normal hexane and ethyl acetate, evaporating to remove the solvent, dissolving base oil and the like. Compared with an extraction process without microbial fermentation assistance, the extraction yield of the paste essential oil extract is remarkably improved, and the aroma intensity of the liquid essential oil with the same volume obtained from the exocarpium leaves with the same mass is also remarkably improved. The essential oil obtained by the invention is suitable for the fields of medicines, foods, daily chemicals and the like.

This invention discloses a plant essential oil composition with anti-Candida activity and its preparation method, which consists of a heavy oil component of Sichuan pepper and a light oil component of dried ginger in a volume ratio of 1:8 to 2:1. Research in this invention has found that, after specific volume ratios, particularly when the heavy oil component of Sichuan pepper or the light oil component of dried ginger is in a volume ratio of 1:8, it exhibits a synergistic effect against Candida albicans, Candida tropicalis, and Candida glabrata; when the heavy oil component of Sichuan pepper or the light oil component of dried ginger is in a volume ratio of 2:1, it exhibits a very good synergistic effect against Candida parapsilosis. This invention allows the heavy oil component of Sichuan pepper and the light oil component of dried ginger to be formulated into an anti-Candida drug in a specific volume ratio.

The invention relates to the technical field of biological medicine, in particular to an antifungal pharmaceutical composition based on a (1, 3)-beta-D-glucan synthase inhibitor. In-vitro experiment results show that the antibacterial peptide LL-37 and (1, 3)-beta-D-glucan synthase inhibitors (caspofungin, micafungin, anidulafungin and iremifungin) are combined for use to achieve the effect of synergistically inhibiting candida albicans, candida auricula, candida tropicalis, candida glabrata, candida parapsilosis and candida krusei, so that the antibacterial peptide LL-37 can be combined with the (1, 3)-beta-D-glucan synthase inhibitors (caspofungin, micafungin, anidulafungin and iremifungin) to achieve the effect of inhibiting the (1, 3)-beta-D-glucan synthase inhibitors). And 3) preparing the antifungal drug by combining the 1, 2, 4, 5, 6, 7, 8, 8, 9, 10- However, the antibacterial peptide LL-37 is easily degraded by in-vivo protease, so that the expression of the antibacterial peptide LL-37 in an organism is up-regulated by adopting an antibacterial peptide LL-37 expression accelerant, and the antibacterial peptide LL-37 and a (1, 3)-beta-D-glucan synthetase inhibitor are synergistically antibacterial in the organism.

The present invention relates to microbiological methods and media dedicated for the non-denaturing detection, differentiation and / or identification of Candida spp. Yeasts, in particular Candida auricula, Candida albicans, Candida glabrata, Candida tropicalis, Candida parapsilosis and Candida krusei. The invention more specifically relates to a method for identifying a yeast of the genus Candida, optionally present in a biological sample, comprising the steps of: culturing the yeast to be identified on or in a culture medium comprising:-a nutritional ingredient capable of allowing the development and growth of a yeast of the genus Candida; and-an alpha-glucosidase inducer and, optionally, an alpha-glucosidase repressor; -an acid phosphatase inducer and a phosphatase repressor; a-N-acetyl-beta-glucosaminase inducer, and a method for producing the same; and-detecting any alpha-glucosidase, acid phosphatase and / or N-acetyl-beta-glucosaminase activity expressed by said yeast; and-identifying the species to which said yeast belongs, based on the activity of alpha-glucosidase, acid phosphatase and / or N-acetyl-beta-glucosaminase actually expressed by said yeast.

The application discloses a beta-1, 6-glycosidic bond hydrolytic enzyme and application thereof in traditional Chinese medicine resources. The method screens a key gene sequence with a function of hydrolyzing beta-1, 6-glycosidic bond from a Candida parapsilosis L7 genome, and names the key gene sequence as CoExg1. A gene engineering bacterium capable of efficiently heterologously expressing CoExg1 is constructed by using a molecular cloning technology. After fermentation and enzyme production, an enzyme catalytic reaction system is established, and a traditional Chinese medicine active molecule in mogroside V is directionally converted, beta-1, 6-glycosidic bonds in different regions of a substrate structure are selectively hydrolyzed, and therefore, efficient and phased synthesis of different products such as sianoside I and mogroside IIIE is realized. In addition, CoExg1 can also hydrolyze beta-glycosidic bonds in the structures of icariin and lupeol, and directionally synthesize icariin II and lupeol, thereby providing important technical support for high-value utilization of traditional Chinese medicine resources and quality improvement and efficiency enhancement of traditional Chinese medicine glycosides.

The invention discloses a microbial leavening agent for increasing ham flavor and application thereof, and is characterized in that the microbial leavening agent is at least one of candida parapsilosis and penicillium angustifolium, and the viable count in candida parapsilosis powder and penicillium angustifolium powder is not less than 1 * 10 < 6 > CFU / g or 1 * 10 < 8 > CFU / mL. Further, the microbial leavening agent is prepared by mixing candida parapsilosis yeast powder and penicillium angustifolium powder according to the mass ratio of (1-2): (1-2), and the microbial leavening agent is added into normal-temperature pure water to be stirred and mixed to be uniform, so that a zymophyte solution is obtained; and uniformly spraying 50-300ml of the zymophyte liquid on the surface of the ham after air drying is finished according to the surface area of each square meter of the ham, and then placing the ham in a fermentation warehouse for fermentation for 2-4 months to obtain the flavored ham with meat fat fragrance, mellow fragrance and fresh fragrance. The fat fragrance, the mellow fragrance and the fresh fragrance of the dry-cured ham meat are obviously improved.

The invention belongs to the technical field of in-vitro molecular detection of pathogenic microorganisms, and particularly provides a primer probe group and a reagent for multiple fluorescent PCR (Polymerase Chain Reaction) and / or multiple quantitative digital PCR detection of five candida. The primer probe group comprises specific primer pairs and corresponding probes aiming at candida albicans, candida tropicalis, candida glabrata, candida parapsilosis and candida krusei. Based on the unified combination, two compatible detection paths are established: five-target synchronous qualitative detection can be realized by adopting a multiple fluorescent PCR (Polymerase Chain Reaction) method, and five-target absolute quantitative analysis can also be realized by adopting a multiple quantitative digital PCR method; the two methods can be applied independently or jointly. Through verification, the primer probe group has high specificity; the sensitivity is excellent, and DNA extracted from a clinical sample can be directly detected. The method is simple in detection process and short in time consumption, and an efficient and reliable molecular detection solution is provided for early differential diagnosis and precise medication of clinical invasive candidiasis.

The invention belongs to the field of biological control of postharvest diseases of fruits and vegetables, and particularly relates to a preparation method and application of a sodium alginate-gellan gum based antagonistic yeast bioactive membrane. The preservation number of the candida linangbi is CGMCC (China General Microbiological Culture Collection Center) No.33800 Firstly, a yeast suspension is prepared; then preparing a basic membrane solution from sodium alginate, gellan gum, glycerol and water, and adding the yeast suspension to obtain a bacterial-containing membrane solution; and finally, the fruits and vegetables are sequentially immersed in a mycoderm-containing solution and a calcium chloride solution, and after the fruits and vegetables are taken out and aired, a stable sodium alginate-gellan gum base antagonistic yeast bioactive membrane can be formed on the surfaces of the fruits and vegetables, so that the antibacterial and fresh-keeping purposes of the fruits and vegetables are achieved. The candida linangbi has originality, and meanwhile, the antagonistic yeast is encapsulated by adopting an edible coating film, so that the candida linangbi is safe and environment-friendly, the survival rate of the yeast in the film is high, long-acting release of bacteriostatic activity is realized, the shelf life is prolonged, the edible safety is guaranteed, and the candida linangbi has a wide market application prospect.

The purpose of the present invention is to provide a method for producing hydroquinone that involves an enzyme that can convert p-hydroxybenzoic acid into hydroquinone at higher efficiencies than the 4-hydroxybenzoate 1-hydroxylase possessed by Candida parapsilosis strain CBS604. This purpose is achieved, for example, by a method for producing hydroquinone, the method comprising a step for obtaining hydroquinone by reacting p-hydroxybenzoic acid with a 4-hydroxybenzoate 1-hydroxylase derived from Spathaspora passalidarum.