The invention belongs to the technical field of feed and microbial fermentation, and particularly relates to a preparation method of a sea-tangle, fish meal, brown sugar and ormosia fermented feed for lactating sows. The method comprises the steps of: mixing sea-tangle powder, the fish meal, the brown sugar and ormosia powder in proportion with water to prepare concentrated serous liquid; heating and soaking, disinfecting and cooling, introducing protease generating bacillus subtilis, brewer's yeast and lactic acid bacteria to ferment; then mixing grain raw materials, such as corn powder, smashed rice powder, bean pulp powder, expanded soybean meal, bran, DDGS (Distillers Dried Grains with Soluble), lipid, premix in proportion; dying after fermentation to prepare the sea-tangle, fish meal, brown sugar and ormosia fermented feed for the lactating sows. The feed is rich in peptide, lactic acid and probiotics, has the characteristics of abundant and balanced amino acids, high nutrition, easy for digestion, good taste, low content of anti-nutritional factors and the like, and the lactating sows can be remarkably stimulated to lactate.