Process method for extracting rapeseed globulin and albumin under optimization and assistance of two enzymes
A process method and assisted extraction technology, which is applied in the field of double-enzyme-assisted extraction of rapeseed globulin and albumin, can solve the problems of high production cost and complicated process, and achieve the advantages of short extraction period, low preparation cost and improved extraction rate Effect
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Embodiment 1
[0025] A double-enzyme optimization-assisted extraction method for rapeseed globulin and albumin, comprising the following steps:
[0026] (1) Take the rapeseed raw material and cold press it for preliminary degreasing, then use petroleum ether Soxhlet extraction to remove the fat, the extraction temperature is 45°C, and the extraction time is 7 hours to obtain the defatted rapeseed cake;
[0027] (2) Dried the defatted rapeseed cake at 60°C to constant weight and crushed to obtain crushed rapeseed cake with a particle size of 120-250 μm;
[0028] (3) Mix the crushed rapeseed cake with the aqueous solution at a ratio of 1:10 (w:v), add 200 U / g of pectinase in the crushed rapeseed cake, and bathe in water at 50°C for 1 hour to obtain rapeseed Water enzyme solution a;
[0029] (4) Centrifuge the rapeseed cake water enzyme liquid a (8000r / min, 20min, 5°C), take the supernatant, pass through a 10K ultrafiltration membrane, and collect the components less than 10K, and the rapesee...
Embodiment 2
[0035] A double-enzyme optimization-assisted extraction method for rapeseed globulin and albumin, comprising the following steps:
[0036] (1) The rapeseed raw material was cold-pressed for preliminary degreasing, and then extracted with petroleum ether Soxhlet to remove the oil, the extraction temperature was 45°C, and the extraction time was 8 hours to obtain the defatted rapeseed cake;
[0037] (2) Dried the defatted rapeseed cake at 40°C to constant weight and crushed to obtain crushed rapeseed cake with a particle size of 120-250 μm;
[0038] (3) Mix rapeseed cake crushed material and aqueous solution at a ratio of 1:10 (w:v), add pectinase with a content of 200U / g rapeseed powder, and bathe in water for 1 hour at a temperature of 50°C to obtain rapeseed water enzyme liquid a;
[0039] (4) Centrifuge the rapeseed cake water enzyme liquid a (8000r / min, 20min, 5°C), take the supernatant, pass through a 10K ultrafiltration membrane, and collect the components less than 10K,...
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