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Process method for extracting rapeseed globulin and albumin under optimization and assistance of two enzymes

A process method and assisted extraction technology, which is applied in the field of double-enzyme-assisted extraction of rapeseed globulin and albumin, can solve the problems of high production cost and complicated process, and achieve the advantages of short extraction period, low preparation cost and improved extraction rate Effect

Inactive Publication Date: 2018-11-20
NANJING UNIV OF FINANCE & ECONOMICS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the process is more complicated and the production cost is high

Method used

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  • Process method for extracting rapeseed globulin and albumin under optimization and assistance of two enzymes
  • Process method for extracting rapeseed globulin and albumin under optimization and assistance of two enzymes

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Embodiment 1

[0025] A double-enzyme optimization-assisted extraction method for rapeseed globulin and albumin, comprising the following steps:

[0026] (1) Take the rapeseed raw material and cold press it for preliminary degreasing, then use petroleum ether Soxhlet extraction to remove the fat, the extraction temperature is 45°C, and the extraction time is 7 hours to obtain the defatted rapeseed cake;

[0027] (2) Dried the defatted rapeseed cake at 60°C to constant weight and crushed to obtain crushed rapeseed cake with a particle size of 120-250 μm;

[0028] (3) Mix the crushed rapeseed cake with the aqueous solution at a ratio of 1:10 (w:v), add 200 U / g of pectinase in the crushed rapeseed cake, and bathe in water at 50°C for 1 hour to obtain rapeseed Water enzyme solution a;

[0029] (4) Centrifuge the rapeseed cake water enzyme liquid a (8000r / min, 20min, 5°C), take the supernatant, pass through a 10K ultrafiltration membrane, and collect the components less than 10K, and the rapesee...

Embodiment 2

[0035] A double-enzyme optimization-assisted extraction method for rapeseed globulin and albumin, comprising the following steps:

[0036] (1) The rapeseed raw material was cold-pressed for preliminary degreasing, and then extracted with petroleum ether Soxhlet to remove the oil, the extraction temperature was 45°C, and the extraction time was 8 hours to obtain the defatted rapeseed cake;

[0037] (2) Dried the defatted rapeseed cake at 40°C to constant weight and crushed to obtain crushed rapeseed cake with a particle size of 120-250 μm;

[0038] (3) Mix rapeseed cake crushed material and aqueous solution at a ratio of 1:10 (w:v), add pectinase with a content of 200U / g rapeseed powder, and bathe in water for 1 hour at a temperature of 50°C to obtain rapeseed water enzyme liquid a;

[0039] (4) Centrifuge the rapeseed cake water enzyme liquid a (8000r / min, 20min, 5°C), take the supernatant, pass through a 10K ultrafiltration membrane, and collect the components less than 10K,...

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Abstract

The invention provides a process method for extracting rapeseed globulin and albumin under optimization and assistance of two enzymes. The process method comprises the following steps: after rapeseeddegreasing, extracting protein step by step under the assistance of pectinase and phytase to obtain a 2S albumin solution and a 12S globulin solution; enabling the 2S albumin solution to pass througha 10k ultrafiltration membrane and collecting components less than 10K; and performing alkali extraction and acid precipitation on the 12S globulin solution to obtain 12S globulin. Compared with the prior art, the process method provided by the invention has the advantages as follows; the steps are simple, the extraction cycle is short, the preparation cost is low, high-quality 12S globulin and 2Salbumin can be rapidly and efficiently extracted from rapeseeds in an energy-saving manner, and the process method has an excellent market prospect and high economic benefits and is worthy of popularization and application.

Description

technical field [0001] The invention relates to a process method for double-enzyme optimized auxiliary extraction of rapeseed globulin and albumin, which belongs to the technical field of agricultural by-product processing. Background technique [0002] According to statistics, my country's rapeseed output in 2016 / 17 was 14 million tons, ranking first in the world. The rich low-value cake resources left after rapeseed oil processing are in urgent need of high-value utilization. Rapeseed cake contains about 40% protein, the amino acid balance is better than soybean protein, and the protein titer is comparable to casein. Moreover, with the popularization of "double-low" rapeseed planting and the advancement of protein extraction methods, the utilization rate and food safety of rapeseed cake protein have been greatly improved. [0003] There have been relevant literature reports on the technology of preparing protein from rapeseed cake, such as: Chinese patent (application pu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K1/34C12P21/00
CPCC07K14/415C12P21/00Y02P20/10
Inventor 王立峰王梅徐斐然刘昆仑姚轶俊熊文飞
Owner NANJING UNIV OF FINANCE & ECONOMICS
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