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Preparation method of cow milk production increasing biological fermentation peptide additive, and application method of additive

A biological fermentation and additive technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the problems of low milk production and milk quality of dairy cows, and achieve increased milk production, strong operability, and improved The effect of transforming power

Inactive Publication Date: 2013-08-28
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims to solve the problem of low milk production and milk quality of dairy cows in my country under the straw nutrition mode of feeding cows, and provides a preparation method and application method of a biological fermentation peptide additive for increasing milk of dairy cows

Method used

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  • Preparation method of cow milk production increasing biological fermentation peptide additive, and application method of additive

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specific Embodiment approach 1

[0013] Specific embodiment one: the preparation method of dairy cow milk increasing bio-fermentation peptide additive according to the present embodiment is carried out according to the following steps:

[0014] 1. Activate the Leuconostoc enterococci subsp. enterica strains with MRS medium, then inoculate the culture medium after activation with 5% inoculum and expand the culture medium to obtain the proliferation culture medium; wherein the proliferation medium The formula is made of 0.5% beef extract, 1% glucose, 1% yeast extract, 1% peptone, 0.5% sodium chloride and the rest of distilled water in terms of mass percentage; the fermentation conditions are: culture temperature 28-30°C, pH6.8~7.1, culture time 24~30h;

[0015] Two, the proliferation culture solution is inoculated into the seed culture solution by 10% ratio, the seed culture solution formula is: soybean meal, corn gluten meal and distilled water, wherein the mass ratio of soybean meal and corn gluten meal is 15...

specific Embodiment approach 2

[0020] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the culture temperature described in step 1, step 2 and step 3 is 29°C. Others are the same as the first embodiment.

specific Embodiment approach 3

[0021] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the fermentation time described in step one is 28h. Others are the same as those in Embodiment 1 or 2.

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Abstract

The invention relates to a preparation method of a biological fermentation peptide additive and an application method of the additive, and concretely relates to a preparation method of a cow milk production increasing biological fermentation peptide additive, and an application method of the additive. A problem of low milk yield of cows in a current straw feeding nutrition mode of cows in China is solved in the invention. The preparation method of the cow milk production increasing biological fermentation peptide additive comprises the following steps: 1, carrying out goldbeater's skin subspecies strain activation of Leuconostoc mesenteroides, and carrying out enlarged cultivation to obtain a propagation cultivation solution; 2, inoculating the propagation cultivation solution to a seed cultivation solution; 3, inoculating the seed cultivation solution obtained in step 2 to a fermentation cultivation solution to obtain fermentation products; and 4, drying and crushing the fermentation products for completing the preparation of the cow milk production increasing biological fermentation peptide additive. The application method of the additive is characterized in that the cow milk production increasing biological fermentation peptide additive is mixed with a concentrated feed to obtain a mixture, and the mixture is fed to lactation period cows once in a 200g lactation period / cow / day mode. The additive is applied to the animal husbandry field.

Description

technical field [0001] The invention relates to a preparation method of a biological fermentation peptide additive and an application method thereof. Background technique [0002] China's dairy industry has developed rapidly in the past ten years. In 2012, the number of dairy cows in China reached 15 million. However, there is always a low level of feeding in my country's dairy industry. The milk yield of dairy cows is low. The average yield per unit area is only about 5400 kg. and other issues have seriously affected the healthy development of the dairy industry. However, the average milk yield of dairy cows in developed countries such as the United States has reached 9000-10000kg, the milk protein is 3.2%-3.5%, and the milk fat rate is 3.5%-4.0%. In recent years, the rapid development of my country's dairy cattle breeding industry has greatly promoted the development of dairy product processing industry. According to the statistical data of "China Agricultural Developmen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K1/14A23K1/18A01K67/02C12N1/20C12R1/01
Inventor 高学军李庆章刘营黄建国卢志勇潘洪宝孙喆霍楠骆超超娄丽郝敬友
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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