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243results about How to "Short extraction cycle" patented technology

Method for extracting soybean dietary fibre and soybean protein from soybean residue

The invention relates to a method for extracting soybean dietary fibre and soybean protein from soybean residue, solving the problem of comprehensive use of the side product of soybean residue in soybean. The method comprises the following steps: drying, crashing and sieving fresh soybean to obtain soybean powder, adding extracting solution containing cellulase into the soybean powder for synchronous ultrasound extraction to obtain extracting solution; centrifuging the extracting solution to obtain supernatant A and sediment B; depositing, drying and crashing the supernatant A to obtain soluble dietary fibre; dissolving the sediment B by weak base to obtain supernatant C and sediment D; depositing, drying and crashing the supernatant C by food acid to obtain soybean protein; washing, decolouring, drying and crashing the sediment D to obtain insoluble dietary fibre. The invention utilizes ultrasonic and enzymolysis synergy food acid to obtain soybean soluble dietary fibre, soybean insoluble dietary fibre and soybean protein, and has the advantages of short extraction time, high product yield, simple technical process, easy operation control and convenience for large-scale industrial production.
Owner:黄山佳龙绿色食品有限公司

Method for preparing undenatured collagen from fish scale of fresh water fish

InactiveCN101307348ASimplify and shorten extraction cyclesShort extraction cyclePeptide preparation methodsFermentationWater bathsIce water
The invention belongs to the agricultural natural product extraction field, in particular relating to a method for extracting unmodified collagen from freshwater fish scale. The method comprises the following steps that: (1) cleaned scale is added in distilled water and is stirred intermittently by a motor stirrer for 3 to 5 minutes till the surface of the scale has no attachment of pigment and fat, etc.; the scale is crushed to 40 to 50 meshes after natural air drying and low-temperature drying; (2) the materials of the step (1) are added into 8 to 10 percent of citric acid and malic acid solution according to a ratio of between 1 to 10 and 1 to 20 so as to carry out ice-water bath microwave treatment for 2 minutes and decalcification at a temperature of between 10 and 20 DEG C for 8 to 24 hours; then, the solution is filtered and the filtrate undergoes standing at a temperature lower than 10 DEG C for 5 to 10 hours so as to obtain calcium citrate precipitate; (3) according to a ratio of between 1 to 5 and 1 to 20, 1 percent malic acid or citric acid solution (containing protease) is added into the de-calcification scale obtained after the treatment of step (2), and is stirred and extracted 2 to 4 times at a temperature of between 0 and 20 DEG C with each time lasting 12 to 48 hours; then, centrifugation is carried out, and supernatant is taken out; NaCl is added till final concentration reach 0.7 to 0.90 mol / L, and then salting out is carried out over night; centrifugation and deposition are carried out, and the obtained substance is dissolved in 0.50mol / L acetic acid; then, salting out and dissolving operations are repeated one more time, and the precipitate undergoes dialysis so as to obtain the product of the invention through freeze drying.
Owner:HUAZHONG AGRI UNIV

Method for extracting purple potato pigment by using ultrasonic wave and compound enzyme

The invention relates to a method for extracting purple potato pigment by using ultrasonic wave and compound enzyme, which includes steps of (1) preprocessing raw material; (2) extracting the purple potato pigment by using ultrasonic wave and compound enzyme; and (3) refining extract by using macroporous resin. By using ultrasonic wave extraction technology, the present invention accelerates diffusion speed of educt from the raw material to solution, and has characteristics high extraction efficiency, no need of high temperature low energy consumption, and greatly shortened extraction time, the enzyme has characteristics of high catalytic efficiency, strong effect specificity and mild catalytic condition, and can increase productivity, lower energy consumption, reduce pollution, simplify process when being used in industry. By combining the ultrasonic wave extraction technology and the biological enzyme technology, the invention has advantages of short extraction time, high production yield, simple process, easy control of operation, convenience for large scale industrialization production and obvious economic benefit.
Owner:HEFEI UNIV OF TECH

Microwave-assisted method for extracting tea saponin

The invention discloses a microwave-assisted method for extracting tea saponin. The microwave-assisted method for extracting the tea saponin specifically comprises the steps of smashing and drying defatted camellia seed meal, sieving and then dissolving the defatted camellia seed meal into organic solution, performing microwave radiation processing, filtration concentration and drying to obtain crude tea saponin, preparing crude tea saponin aqueous solution, performing flocculation, precipitation, transformation and decoloration processing and finally performing drying to obtain a refine tea saponin product. Compared with an existing tea saponin extracting method, the microwave-assisted method for extracting the tea saponin has the advantages of being short in period, high in efficiency, simple in operation, small in usage mount of the organic solution, low in consumption, high in purity of produced tea saponin and the like.
Owner:ZHEJIANG SCI-TECH UNIV

Method for extracting and separating isoflavone from kudzu

InactiveCN1769278AReduce consumptionThe ecological environment has a huge effectOrganic chemistryDaidzinEcological environment
The invention discloses a process for extracting and separating isoflavones from kudzu vine, which comprises the steps of disintegrating kudzu vine to 60-150 meshes, loading into a liquid dynamic continuous flow upstream extraction device for alcohol extraction, combining the extract liquid and filtering, concentrating the filtrate to obtain total isoflavones extract, and separating the total isoflavones extract to obtain puerarin and soybean aglycone.
Owner:ZHEJIANG UNIV OF TECH

Method for extracting, separating and purifying formononetin and calycosin from Astragalus mongholicus waste residue

The invention provides a method for extracting, separating and purifying formononetin and calycosin from Astragalus mongholicus waste residue. The technical scheme adopted in the invention is as follows: taking waste residue after the production and process of astragalus injection as a raw material; adopting a series of original and efficient technologies for extracting, separation and purifying such as homogenate extraction-mixing enzyme induction biotransformation technology, negative pressure cavitation extract technology, liquid-liquid extraction technology, macroporous absorption resin enrichment technology, normal phase silica gel medium pressure column chromatography technology and devitrification at a low temperature, recrystallization technology and the like to obtain the formononetin and calycosin with high purity, wherein the purity thereof can be more than 95%, and the yield is 80-95%. The raw material used in the invention is the waste residue in the industry production, and the process of the method is simple and practicable, has small pollution to the environment; the obtained formononetin and calycosin have low production cost, high purity and yield, good repeatability, high utilization effect of Astragalus mongholicus resources and the like; and the method is suitable to scaled industrial production.
Owner:NORTHEAST FORESTRY UNIVERSITY +1

Method for purifying and separating phenolic compounds in magnolia medicament

The invention relates to a method for extracting and separating phenolic compounds of magnolia through supercritical CO2 liquid technology. The method comprises the following steps that: magnolia is pulverized into dry powders with 20 to 60 meshes; the dry powders are thrown into an extraction kettle; the extraction kettle, a resolution kettle I and a resolution kettle II are heated respectively; carbon dioxide is injected to the extraction kettle and two resolution kettles through a high-pressure pump; the flow speed of the carbon dioxide liquid and the flow speed of an entrainer are adjusted to begin extraction, and the constant temperature and pressure and the stability of a system are kept for circular extraction; after every circulation for thirty minutes, the materials are discharged from the resolution kettle I and the resolution kettle II to obtain a flaxen extractant; the flaxen extractant obtained by the resolution kettle I and the resolution kettle II is dissolved by petroleum ether in proper amount, kept stand and filtered; the petroleum ether is reclaimed from the filtrate; the filtrate is recrystallized by a recrystallization solvent to obtain magnolol and mixed total phenol of the magnolol; the mixed total phenol is recrystallized to obtain a magnolol crystal; and after a mother solution is concentrated, the mother solution is recrystallized by the recrystallization solvent to obtain the magnolol crystal.
Owner:GUANGZHOU HONSEA SUNSHINE BIOTECH CO LTD

Continuous gradient counterflow extraction producing soybean concentrate protein method and equipment

The invention discloses a continuous gradient countercurrent extraction method for producing soybean protein concentrate, which takes low-temperature defatted soybean expeller or high-temperature defatted expeller as the raw materials, and ethanol solution as the extraction agent; a multilayer and continuous extraction equipment is adopted for extracting the defatted soybean expeller continuously with a completed closure operating technology, and a soybean molasses is obtained from the extraction liquid which is made by the extraction technology. The soybean protein concentrate made by the method of the invention has light white color and pure flavor without salty taste, which can eliminate the anti-nutritional factors, and can be widely used in the food industry, which also is an indispensable nutritional additive in high-grade feed. The soybean molasses production which is made by the method of the invention is the best raw materials for further processing soybean isoflavones, soybean saponins, soybean oligosaccharides and extracting trypsinase inhibitor.
Owner:黑龙江双河松嫩大豆生物工程有限责任公司

Extracting process for coral fungi polysaccharide

InactiveCN101475649ADoes not destroy the three-dimensional structureIncrease movement speedImmunological disordersAntineoplastic agentsAlcoholMicrowave
The invention discloses an extracting process for a club fungi polysaccharide. The extracting process comprises the following steps: carrying out the pretreatment of raw materials, carrying out the hot water leaching, ultrasonic extraction or microwave extraction, and storing the extract after concentration, alcohol deposition, centrifugation and drying, wherein the hot water leaching method has moderate conditions and simple operation; and the ultrasonic extraction method and the microwave extraction method can save time and improve the extraction efficiency. The extracted club fungi polysaccharide has an inhibition rate of 70 percent for little white rat S-180 and Ehrlich carcinoma, and has the functions of improving the immunity, diminishing inflammation, easing pain, and the like.
Owner:夏晖

Production process of decaffeination tea

The invention discloses a production process of decaffeination tea, which is characterized in that a supercritical carbon dioxide extraction method is utilized for producing the decaffeination tea, water is added into the supercritical carbon dioxide for preparing entrainers, 90 percent of caffeine in the tea can be extracted in a short time at a low temperature, the fragrance and tea polyphenol in the tea can be effectively protected, and the invention has the characteristics of high emersion rate, short extraction period, pure natural effect, no pollution and the like.
Owner:GUIZHOU TAIBO BIO TECH

Mobile robot for dangerous chemical solution extraction

The invention relates to a mobile robot for dangerous chemical solution extraction. The mobile robot comprises a mobile trolley system, a multi-joint mechanical arm system, a fixture system, a vision ultrasonic testing system and a dangerous chemical solution storage system, and the multi-joint mechanical arm system, the fixture system, the vision ultrasonic testing system and the dangerous chemical solution storage system are all arranged on the mobile trolley system; the mobile trolley system is connected with the multi-joint mechanical arm system and the dangerous chemical solution storage system; the multi-joint mechanical arm system is connected with the fixture system; and the mobile trolley system and the vision ultrasonic testing system are both in communication connection with a main console system. By means of the mobile robot, precise extraction of a dangerous chemical solution and automatic treatment of a surplus dangerous chemical solution can be achieved, middle processes are decreased, and the time cost, the labor cost and the management cost are reduced.
Owner:中国东方电气集团有限公司

Novel method for effectively extracting lentinan

The invention discloses a novel method for effectively extracting lentinan, wherein lentinus edodes sporophores or lentinus edodes stems are taken as a raw material, a subcritical water extraction technology is adopted, coarse lentinan is obtained after the processes of ultrafiltration concentration, ethanol precipitation and decolorization drying, protein is removed by anion exchange resin, and the purity of the refined lentinan can reach above 90%. By utilizing the method, the extraction period is greatly shortened, the active ingredient activity is maintained to the maximum extent, the extraction efficiency can be obviously increased; and moreover, compared with other extraction methods, the method has the advantages of no toxity, no harm, energy conservation and environmental friendliness as well as wide application prospect.
Owner:胡玮

Method for preparing schaftoside from desmodium styracifolium

The invention discloses a method for preparing schaftoside from desmodium styracifolium. The method comprises the following steps of: i, crushing the desmodium styracifolium, adding biological enzyme and uniformly stirring, performing enzymolysis at normal temperature for 4-7 days, soaking enzymolysis raw material with warm water and extracting for two to three times, filtering extract and ultra-filtering with an ultrafiltration membrane, concentrating permeate with a nanofiltration membrane, extracting the concentrate by using acetic ether and concentrating the extract to obtain a rough extract; and ii, separating the rough extract by using high-speed counter-current chromatography, performing on-line monitoring through an ultraviolet detector, collecting a target component and performing reduced pressure drying to obtain the schaftoside. The method has the advantages of simple operation, high efficiency, large preparation capacity and high purity of an obtained product.
Owner:NANJING ZELANG MEDICAL TECH

Method for extracting high-density lipoprotein and separating and purifying apolipoprotein apoA-I from human plasma

The invention provides a method for extracting high-density lipoprotein (HDL) and separating and purifying high-purity apolipoprotein apoA-I from normal human plasma. The method comprises the following steps: (1) regulating plasma to be of different densities by utilizing neutral salt, and eliminating impure protein by utilizing a sequential gradient density supercentrifugation method, so as to obtain a high-purity HDL component; (2) carrying out degreasing on the obtained high-purity HDL component in a flowingly adding manner by utilizing a certain proportion of alcohol ether solvents under a low-temperature freezing state, so as to obtain degreased apoHDL precipitates; (3) redissolving the apoHDL precipitates by utilizing a TBS buffer liquid system containing 1-8M urea, filtering, carrying out separation and purification by molecular sieve chromatography of filtrate and anion exchange chromatography in sequence to obtain a high-purity apoA-I solution. According to the method, albumin components which are very difficult to remove can be separated from the HDL; the apoA-I prepared by utilizing the method has very high purity and activity, the purity can reach above 98%, and the apoA-I can generate clear precipitin reaction with antiserum after being diluted by 64 times; the method is safe and convenient to operate, is short in extraction period and is suitable for industrial production.
Owner:SHANGHAI FOSUN PHARMA (GROUP) CO LTD +1

Method for extracting total flavonoids from cloverleaf by supercritical carbon dioxide

The invention discloses a method for extracting total flavonoids of A. trifoliate with supercritical carbon dioxide, which is characterized in that: A. trifoliate medicinal material is crushed into 60-80 meshes, added to a supercritical carbon dioxide extraction tank, fed with liquid carbon dioxide and an entrainer, and extracted at a pressure of 20 Under the conditions of -30MPa and temperature 40-50°C, extract for 2-3 hours, analyze the extract under reduced pressure in a separator, recover the reagent, and dry to obtain the total flavonoids of Clover. The method of the invention is simple in operation, high in efficiency, can reduce pollution, reduce the consumption of organic solvents, and is suitable for industrialized production.
Owner:NANJING ZELANG MEDICAL TECH

Process for preparing compound folium isatidis injection with membrane filter technique

The invention discloses a process for preparing compound dyers woad leaf injection by membrane filtration technology. The process for preparing the compound dyers woad leaf injection by membrane filtration technology is as follows: according to the prescription of the compound dyers woad leaf injection, medicine materials are decocted; medicine liquid is condensed to three times as much as formula dosage, centrifuged, filtrated directly by the membrane with large pore size, filtrated by an ultrafiltration membrane and condensed by a nanofiltration membrane, thus extracting the compound dyers woad leaf injection. The process for preparing the compound dyers woad leaf injection has following characteristics: 1. heating process is reduced to decrease the loss of effective components; 2. the process is carried out at normal temperature to prevent phase transition and pollution; 3. the ethanol extraction process in conventional technology is replaced, so cost is saved and production is kept clean; 4. extraction period is shortened and pollution sections are reduced, thus offering technological insurance for the quality of products; 4. compared with content produced by prior art, the content is increased by over two times.
Owner:SHANDONG HUAXIN PHARMA

Preparation method of manyprickle acanthopanax root extract

The invention relates to a preparation method of traditional Chinese medicine extract, in particular to a preparation method of extract of medicine material manyprickle acanthopanax root. The manyprickle acanthopanax root extract is prepared through the procedures of conducting countercurrent extracting through ethyl alcohol with concentration of 40-70%, alcohol precipitating, resin purifying, sterilizing, concentrating and the like on medicine material manyprickle acanthopanax root. By means of the method, impurities in the manyprickle acanthopanax root extract can be remarkably reduced, the purity of effective components in the manyprickle acanthopanax root extract and the safety and stability of the manyprickle acanthopanax root extract are improved, the extract cycle can be shortened, energy conservation and high efficiency are achieved, and the method is particularly suitable for industrial production.
Owner:HARBIN ZHENBAO PHARMA

Method for extracting hypericin

This invention discloses hypericin distillation method. It includes following procedures 1. Leaf weeping forsythia is dried and pulverized. 2. The forsythia powder is mixed with organic solvent as volume ratio 1-2 t 3, then they are distilled at 40-70 degree centigrade for 2-4 hours, extracting solution is collected, filtrated and filtration liquor is collected. 3. The filtration liquor is decompressed and concentrated, concentrated solution is recovered. 4. The liquor is concentrated by resin, and then eluted, eluent is collected. 5. The eluent is decompressed and concentrated, and then hypericin extractive is got. In this invention its operation is simple, easy for industrial application; its distillation cost is low. The extracting solution toxity is little, distilling period is short. Hypericin content in extract is high. So this invention takes important part in hypericin organism distillation.
Owner:LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS +1

Method for extracting crude nemadectin product from nemadectin fermentation liquor

The invention relates to a method for extracting a crude nemadectin product from nemadectin fermentation liquor, which comprises the following process steps: firstly, carrying out plate frame filter and flash drying on the nemadectin fermentation liquor so as to obtain dry nemadectin dregs; then, leaching the dry nemadectin dregs by using low alcohols as a solvent, carrying out falling film concentration on leaching liquor until no liquid flows out; adding water for acidifying until the pH value of the obtained product is 4.5-5, carrying out decoloring treatment by using macroporous resins, carrying out secondary countercurrent extraction, and reextracting; and finally, carrying out resin adsorption, and crystallizing the obtained product, thereby obtaining the crude nemadectin product. According to the invention, a preparation method for increasing the yield of nemadectin, reducing the cost, and improving the quality of crystals and finished products of nemadectin is implemented, and the method is suitable for industrial production.
Owner:宁夏泰瑞制药股份有限公司

Production method for extracting glucoside type seabuckthorn flavones from sea-buckthorn leaf

The invention discloses a production method for extracting glucoside type seabuckthorn flavones from sea-buckthorn leaves and the method comprises the following steps: carrying out pre-processed machining, such as cleaning, jordaning and the like to fresh sea-buckthorn leaves or picked fallen leaves; taking ethanol as solvent; and under the action of ultrasonic wave, further breaking plant cells to release glucoside type seabuckthorn flavones-Isorhamnetin, wherein the extraction ratio is 95.6%. Compared with the traditional method, the production method disclosed by the invention has the advantages of low temperature, constant pressure, no toxic solvent residue, low energy consumption, short extraction period and high extraction efficiency, does not damage or degrade effective ingredientsand is suitable for mass production; and the additional value of agricultural products is improved.
Owner:XINJIANG KEYU TECH

New process for extracting stevioside by utilizing enzyme coupling membrane separation technology

The invention discloses a new process for extracting stevioside by utilizing an enzyme coupling membrane separation technology, belonging to the technical field of food additives. The stevioside is a natural sweetener extracted from stevia rebaudian leaves, and the characteristics of high sweetness and low heat energy of the stevioside are more and more accepted by people. The existing extraction process has the defects of long extraction period, low extraction efficiency and the like, which is not favourable for the development of the stevioside industry. In the new process, the stevioside leaves serve as raw materials and are extracted at low temperature by an enzymatic method; impurities, such as colloid, pigment and the like, in the extracting solution are removed by virtue of a flocculation process; then, membrane filtration treatment is carried out by an ultrafiltration membrane with certain molecular weight cut-off; the ultrafiltrate is adsorbed, desorbed and decolorized by utilizing a resin coupling technology; and finally, the finished product is obtained by virtue of spray drying. The high-efficiency liquid chromatography proves that the total content of the nucleosidein the product obtained by the new process is above 98% which is superior to those of the international super-grade products. According to the new process, the problems of long production period, low stevioside transfer rate and the like of the conventional method can be effectively solved, and the resin service life is prolonged by more than 6 times.
Owner:田景振

Swida wilsoniana oil extracting method

The invention discloses a swida wilsoniana oil extracting method which comprises the following steps: firstly, washing a fresh cornus wilsoniana fruit to remove the impurities on the surface of the fresh cornus wilsoniana fruit; secondly, carrying out microwave baking pretreatment on the washed fresh cornus wilsoniana fruit; thirdly, crushing the pretreated fresh cornus wilsoniana fruit into pulp, adding water to extract, and using ultrasonic waves to assist extract; fourthly, removing fruit residues in the extract liquid through centrifugal decantation; fifthly, centrifugally separating the mixed liquid to obtain the swida wilsoniana oil. The swida wilsoniana oil extracting method applies the two physical extracting techniques of microwaves and ultrasonic waves into the extraction of the swida wilsoniana oil, so that the extraction period is greatly shortened, the extraction efficiency is improved, water is used as a solvent in the extracting process, safety and zero pollution are guaranteed, the production cost is lowered, the process is simple, the conditions are mild, the extracted swida wilsoniana oil is high in quality and nutritional value.
Owner:江西省粮油科学技术研究所

General flavone of root of lilac daphne, method of extraction, and application

A general flavone of lilac daphne root is prepared from lilac daphne root through breaking, proportionally mixing it with alcohol, extracting at 40-70 deg.C for 12-48 hr, removing deposit, adsorbing by macroreticular resin, water washing, eluting with alcohol, vacuum distilling, passing through silicon gel column, eluting with the mixture of chloroform and methanol, and concentrating. It can be used to prepare the medicines for preventing and treating the sexual tumor.
Owner:XUZHOU NORMAL UNIVERSITY

Method for preparing shiny-leaved yellowhorn kernel oil by adopting ultrasound-assisted salt process

The invention discloses a method for preparing shiny-leaved yellowhorn kernel oil by adopting the ultrasound-assisted salt process and belongs to the field of food and healthcare food. The method comprises the following steps of: freezing and drying shiny-leaved yellowhorn kernels as raw materials at low temperature in vacuum, pulping, mixing pulps, extracting by promoting dissolving by adopting the ultrasound-assisted salt process, and centrifuging at high speed to obtain the high-quality shiny-leaved yellowhorn kernel oil. Because the shiny-leaved yellowhorn kernels are frozen and dried at low temperature in vacuum, the original components of the shiny-leaved yellowhorn kernels are protected from damage. Because the technology of extracting by promoting dissolving by adopting the ultrasound-assisted salt process is used for demulsifying, the oil can be extracted to the maximum extent, high oil yield can be achieved, and the problem of serious common water extraction and emulsification can be solved. Because the ultrasound-assisted salt process is used for extracting, the extracting cycle can be shortened, the energy consumption can be reduced, and high efficiency can be achieved. The method has the advantages that the reaction condition is mild, and the extracting process is environment-friendly.
Owner:NORTHWEST NORMAL UNIVERSITY

Method for quickly extracting bacteria cellulose from mother liquor

This invention discloses a process for the separation and purification of bacteria cellulose from mother liquid, which comprises the following steps: putting wet bacteria cellulose film into distilled water, formulating 1-9% alkali liquor and 1-10% acid solution by concentration,separetly holding temperature to 20-60 degrees with digital display thermostatic and magnetic stirrer; putting wet bacteria cellulose film into alkali liquor formulated for swelling for 3-10min, then taking it out and putting it into acid solution formulated, steeping it for 4-8min with magnetic stirrer whose revolution rate is 100- 700rpm; taking the wet bacteria cellulose film and rinsing it for 2-3 times, then conserving it in fridge. The invention comprises the following good effects: (1) using not only alkali liquor, but also acid solution; (2) the temperature isn't high and the reaction time isn't long in purification process; (3) the solution used is nonvolatile, so the process is nontoxic; (4) the solution can be used repeatedly, so the extraction cycle is shortened.
Owner:DONGHUA UNIV +1

Supercritical extracting method of ginger oil

The invention discloses a supercritical extracting method of ginger oil. The ginger oil is extracted from ginger by adopting supercritical carbon dioxide fluid. Before extracting, ginger is selected,washed, dried and pulverized, the extracting temperature is 25-65 DEG C, and the pressure is 15-28 MPa; the flow of carbon dioxide gas passing through an extracting kettle is 1.0-1.3 t / h, and the extracting time is 2-4 h. The carbon dioxide fluid under the supercritical state is used as an extracting solvent, the solvent is recycled in the same system, the solvent consumption is small, the price is cheap, and thus the extracting cost is low. The carbon dioxide fluid is green pollution-free solvent, the extracted ginger oil has the advantages of being high in quality and purity, good in color,free of peculiar smell, solvent residue and environmental pollution and the like, the extracting cycle is short, residues after the ginger oil is extracted also contain effective components like starch and dietary fiber, and the ginger can be comprehensively utilized. The technology is simple, and the energy consumption is low.
Owner:GUIZHOU AEROSPACE WUJIANG MACHINERY & ELECTRICITYEQUIP

Extracting method for deer skin collagens

The invention discloses an extracting method for deerskin collagens. The extracting method comprises the following steps: dehairing the skin of a sika deer or the skin of a deer skin, cutting into small pieces, disintegrating by a high-speed tissue disintegrator, and removing liposoluble components by a supercritical CO2 extracting method to obtain the fat-removed deer skin; adding a NaC1 solution in the fat-removed deer skin, continuously pumping feed liquid into a high-voltage pulse extraction device by a liquid pump to obtain an impurity-removing protein precipitation, washing the protein precipitation with distilled water, centrifuging, removing a water layer to obtain a desalted precipitation, adding an organic acid solution in the desalted precipitation, adding a protease, continuously pumping feed liquid into the high-voltage pulse extraction device by the liquid pump, continuously collecting an extracting solution, centrifuging, concentrating a supernatant to a certain volume, and performing freeze drying to obtain a white solid. The extracting method has the advantages that thermosensitive components are invariable, the extraction is continuous and fast, the technology is advanced, and environment protection and high efficiency are achieved.
Owner:王全凯

Preparation method of bletilla striata polysaccharide

The invention belongs to the technical field of biology, and particularly relates to a preparation method of bletilla striata polysaccharide. The preparation method comprises the following steps: drying bletilla striata tuberous roots, crushing and sieving; adding the bletilla striata powder into the prepared low-molecular-weight alcohol / salt aqueous two-phase system solution, performing microwave-assisted extraction, performing high-speed centrifugal separation to obtain a lower phase, performing ultrafiltration, concentration and desalination, and performing freeze drying to obtain bletillastriata polysaccharide. Compared with the prior art, the method can effectively shorten the extraction time, improve the extraction purity and reduce the extraction steps, and is simple and convenientto operate and easy for industrial scale preparation. The prepared bletilla striata polysaccharide is complete in structure, high in polysaccharide yield, high in protein removal rate, low in toxicity and safe, can prevent skin barrier damage and promote the repair process of the skin barrier damage, and has a good anti-allergic effect on sensitive skin with abnormal skin barrier functions.
Owner:澜海(福建)生物科技有限公司

Improved method for extracting berberine

The invention belongs to the field of extraction of plant active ingredients and relates to an extraction purifying method for removing plant cell walls with cellulase and breaking cells accordingly, in particular to an improved method for extracting berberine. The improved method includes crushing golden cypress, washing and steaming, subjecting the crushed golden cypress to reaction with enzyme, digesting by lime milk, depositing and crystallizing, drying and the like. The improved method for extracting berberine is high in digestion rate, short in extraction period, and low in solvent usage. With degradation of cell walls by cellulase, the cell walls are broken in different degrees, permeability of cells is improved, digestion rate of active components in the cells is increased, extraction time is shortened, and further, usage of the solvent is lowered, and production efficiency is improved. Besides, the method is energy-saving, environment-friendly and safe. Enzyme reaction is mild in conditions, requirements for engineering equipment are not high, and operation is convenient; materials required by the enzyme reaction causes no pressure to the environment, energy consumption and pollution to the environment are reduced.
Owner:SICHUAN AGRI UNIV +1

Method for producing lycopene by blocking metabolic pathway of Dunaliella bardawil

The invention discloses a method for producing lycopene by bloking the metabolic pathway of Dunaliella bardawil. The method comprises the following steps: preparing a Dunaliella bardawil basic culturing solution, adding an A5 trace element solution to the culturing solution, adjusting the pH value, disinfecting, and cooling to room temperature; and 2, adding Dunaliella bardawil cells to the culturing solution, culturing in an illumination incubator, adding 200-800ppm of a blocker triethylamine when OD600 reaches 0.7, continuously culturing for 24-72h, and extracting a pigment to obtain lycopene. Compared with traditional methods for extracting lycopene from tomato skins, the extraction method disclosed in the invention has the advantages of simplicity, convenience, fastness and short extraction period. Compared with Blakeslea trispora and other autotrophic microorganisms, Dunaliella bardawil has the advantages of large-scale culture benefiting, simple requirements of environment required by culturing, and according with industrial large-scale production standards.
Owner:SOUTH CHINA UNIV OF TECH
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