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Extracting method for deer skin collagens

A kind of deerskin collagen, collagen technology, applied in the field of biomedicine

Active Publication Date: 2015-01-28
王全凯
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Collagen has good biocompatibility and weak antigenicity, and is widely used in the fields of health care products, pharmaceutical excipients and biological materials. Conventional collagen extraction methods include acid method, alkali method, thermal degradation method, enzymatic method and these methods Combined use, etc., in the extraction process, there are problems such as long process cycle, complex process steps, and low extraction rate. Gomez-Guillen et al. studied the impact of high-pressure treatment on the collagen extraction process. In addition, pretreatment methods such as ultrasound and pressure pulse Also tried for collagen extraction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Deerskin pretreatment: take fresh sika deerskin, remove hair, scrape off grease, wash, cut into small pieces of 1.0cm×1.0cm, wash with 4°C distilled water until bloodless, drain, and take 1.0kg of deerskin for later use; Degreasing: take the deerskin, crush it with a high-speed tissue grinder, put it in a supercritical CO2 extraction kettle for extraction at 25MPa 40°C for 1 hour, and take 0.85kg of deer antler for later use; Remove impurity protein: Take 0.85kg of degreased buckskin and add 4.25L 5% NaCl solution to mix evenly, and use a liquid pump to continuously pump the material liquid into the high-voltage pulse extraction device. Feed speed, 5ml / min is the parameter extraction, continuously collect the extract, centrifuge, discard the supernatant to obtain the precipitate that removes impurities; the precipitate is washed with 25.5L distilled water, centrifuged, and the water layer is discarded to obtain 0.70kg of the precipitate after desalination. Collagen...

Embodiment 2

[0011] Deerskin pretreatment: take fresh sika deerskin, remove hair, scrape off grease, wash, cut into small pieces of 1.0cm×1.0cm, wash with 4°C distilled water until bloodless, drain, and take 3.0kg of deerskin for later use; Degreasing: take the deerskin, grind it with a colloid mill, put it in a supercritical CO2 extraction kettle for 5 hours at 30MPa and 35°C, and extract 2.5kg of deer antler for later use; Remove impurity protein: Take 2.5kg of degreased deerskin and add 75L 1% NaCl solution to mix well, use a liquid pump to continuously pump the material liquid into the high-voltage pulse extraction device, at a temperature of 20°C, a field strength of 10KV, and a pulse number of 5, the material is fed Speed, 10ml / min is the parameter extraction, continuously collect the extract, centrifuge, discard the supernatant to get the precipitate to remove impurities; wash the precipitate with 25L distilled water, centrifuge, discard the water layer, and get 2.2kg of the prec...

Embodiment 3

[0013] Deerskin pretreatment: take fresh sika deerskin, remove hair, scrape off grease, wash, cut into small pieces of 1.0cm×1.0cm, wash with 4°C distilled water until bloodless, drain, and take 5.0kg of deerskin for later use; Degreasing: take deerskin, crush it with a high-speed tissue grinder, put it in a supercritical CO2 extraction kettle for extraction at 35MPa 40°C for 3 hours, and take 4.2kg of deerskin after extraction for use; Remove impurity protein: Take 4.2kg of defatted buckskin and add 94L 3.0% NaCl solution to mix evenly, and use a liquid pump to continuously pump the material liquid into the high-voltage pulse extraction device. The speed is 15ml / min as the parameter extraction, the extract is collected continuously, centrifuged, and the supernatant is discarded to obtain the precipitate that removes impurities; the precipitate is washed with 100L distilled water, centrifuged, and the water layer is discarded to obtain 3.6kg of the precipitate after desalin...

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Abstract

The invention discloses an extracting method for deerskin collagens. The extracting method comprises the following steps: dehairing the skin of a sika deer or the skin of a deer skin, cutting into small pieces, disintegrating by a high-speed tissue disintegrator, and removing liposoluble components by a supercritical CO2 extracting method to obtain the fat-removed deer skin; adding a NaC1 solution in the fat-removed deer skin, continuously pumping feed liquid into a high-voltage pulse extraction device by a liquid pump to obtain an impurity-removing protein precipitation, washing the protein precipitation with distilled water, centrifuging, removing a water layer to obtain a desalted precipitation, adding an organic acid solution in the desalted precipitation, adding a protease, continuously pumping feed liquid into the high-voltage pulse extraction device by the liquid pump, continuously collecting an extracting solution, centrifuging, concentrating a supernatant to a certain volume, and performing freeze drying to obtain a white solid. The extracting method has the advantages that thermosensitive components are invariable, the extraction is continuous and fast, the technology is advanced, and environment protection and high efficiency are achieved.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for extracting collagen from buckskin by using high-voltage pulsed electric field technology. Background technique [0002] Collagen is a biopolymer synthesized by animal cells, a structural protein that widely exists in organisms, accounting for about 1 / 3 of the total protein in mammals, and is the main component of skin, cartilage, arterial vessel wall and connective tissue , play multiple roles in animals such as support, protection, and connection. Collagen has good biocompatibility and weak antigenicity, and is widely used in the fields of health care products, pharmaceutical excipients and biological materials. Conventional collagen extraction methods include acid method, alkali method, thermal degradation method, enzymatic method and these methods Combined use, etc., in the extraction process, there are problems such as long process cycle, complex process steps, and l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/14
CPCY02P20/54
Inventor 王全凯李庆杰王莲萍胡太超博士儒陶珊
Owner 王全凯
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