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Method for quickly extracting bacteria cellulose from mother liquor

A bacterial cellulose and purification method technology, which is applied in the field of rapid purification of bacterial cellulose, can solve the problems of bacterial cellulose film influence, bacterial cellulose surface structure damage, etc., and achieve the effect of shortening the extraction cycle

Inactive Publication Date: 2006-09-27
DONGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-time immersion and boiling in lye will inevitably damage the surface structure of bacterial cellulose, which will have a series of adverse effects on the bacterial cellulose film.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The bacterial cellulose wet film produced by Acetobacter xylinum was placed in distilled water with a concentration of 5% NaOH solution and 9% H 2 SO 4 Solution, under the action of a digital display constant temperature magnetic stirrer, keep the temperature to 40 ° C, put the bacterial cellulose wet film in the NaOH solution, take it out after swelling for 5 minutes, the lye can be kept for multiple use; then put the bacterial cellulose into 9% H at a temperature of 40°C 2 SO 4 In the solution, turn on the speed control button and adjust the speed to 500rpm and work for 5 minutes. During the rotation of the cellulose, the loose impurities inside will enter the solution under the action of centrifugal force; take out the cellulose wet film and rinse it twice and store it in the refrigerator. The liquid can be slightly adjusted and reused.

Embodiment 2

[0021] The bacterial cellulose wet film produced by Glucobacterium was placed in distilled water, and the concentration was 1% NaOH solution and 1% H 2 SO 4 solution, under the action of a digital constant temperature magnetic stirrer, the temperature was kept at 60°C, and the bacterial cellulose wet film was placed in the NaOH solution, and it was taken out after swelling for 3 minutes. The lye can be kept for multiple use; The temperature is 45°C, the concentration is 8% H 2 SO 4 In the solution, turn on the speed control button and adjust the speed to 700rpm and work for 4 minutes. During the rotation of the cellulose, the loose impurities inside will enter the solution under the action of centrifugal force; take out the cellulose wet film and rinse it 3 times, and store it in the refrigerator. The liquid can be slightly adjusted and reused.

Embodiment 3

[0023] The bacterial cellulose wet film produced by rhizobia was placed in distilled water, and the concentration was 9% NaOH solution and 10% H 2 SO 4 Solution, under the action of a digital display constant temperature magnetic stirrer, keep the temperature to 20°C, put the bacterial cellulose wet film in the NaOH solution, take it out after swelling for 10min, the lye can be kept for multiple use; then put the bacterial cellulose into 9% H at a temperature of 40°C 2 SO 4 In the solution, turn on the speed control button and adjust the speed to 100rpm and work for 8 minutes. During the rotation of the cellulose, the loose impurities inside will enter the solution under the action of centrifugal force; take out the cellulose wet film and rinse it 2 times and store it in the refrigerator. The liquid can be slightly adjusted and reused.

[0024] According to chemical analysis, the purity of bacterial cellulose reaches 99.1%. After slightly adjusting the recovered lye and aci...

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PUM

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Abstract

This invention discloses a process for the separation and purification of bacteria cellulose from mother liquid, which comprises the following steps: putting wet bacteria cellulose film into distilled water, formulating 1-9% alkali liquor and 1-10% acid solution by concentration,separetly holding temperature to 20-60 degrees with digital display thermostatic and magnetic stirrer; putting wet bacteria cellulose film into alkali liquor formulated for swelling for 3-10min, then taking it out and putting it into acid solution formulated, steeping it for 4-8min with magnetic stirrer whose revolution rate is 100- 700rpm; taking the wet bacteria cellulose film and rinsing it for 2-3 times, then conserving it in fridge. The invention comprises the following good effects: (1) using not only alkali liquor, but also acid solution; (2) the temperature isn't high and the reaction time isn't long in purification process; (3) the solution used is nonvolatile, so the process is nontoxic; (4) the solution can be used repeatedly, so the extraction cycle is shortened.

Description

technical field [0001] The invention relates to a method for quickly purifying bacterial cellulose directly taken out from a liquid fermentation medium, in particular to a method for treating cellulose wet film with lye and inorganic acid solution for a short period of time to obtain A method of bacterial cellulose wet film with white and transparent appearance and good purity. Background technique [0002] Bacterial cellulose is a type of cellulose produced by microorganisms. Acetobacter produces bacterial cellulose, which is beneficial to its own life activities. In a liquid environment, the formation of a liquid film on the surface of cellulose can help acetic acid bacteria maintain a low oxygen concentration. Adequate liquid surface for growth. The biosynthesis of bacterial cellulose can be divided into four major processes: polymerization, secretion, assembly and crystallization. These four processes are highly coupled and closely related to specific sites on the cell ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D21C5/00
Inventor 孙晓玉王华平颜志勇石帅科陈仕艳邹瑜王彪张玉梅
Owner DONGHUA UNIV
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