Preparation method of bletilla striata polysaccharide

A technology of bletilla striata polysaccharides and bletilla striata, applied in the biological field, to achieve the effect of preventing damage, good effect, and shortening the extraction cycle

Active Publication Date: 2020-01-07
澜海(福建)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the research on the extraction of Bletilla striata polysaccharides by the two-phase system.

Method used

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  • Preparation method of bletilla striata polysaccharide
  • Preparation method of bletilla striata polysaccharide
  • Preparation method of bletilla striata polysaccharide

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preparation example Construction

[0027] A kind of preparation method of bletilla striata polysaccharide of the present invention specifically comprises the following operation steps:

[0028] (1) Processing of raw materials of Bletilla striata: take the root of Bletilla striata and place it at a temperature of 60°C-80°C to dry to constant weight, and the dried root tuber is pulverized by a high-speed tissue grinder, and sieved to obtain Bletilla striata powder of 20 mesh to 80 mesh;

[0029] (2) Preparation of low-molecular-weight alcohol / salt two-phase system: prepare low-molecular-weight alcohol and inorganic salt two-phase system at room temperature, accurately weigh the inorganic salt, add an appropriate amount of water to prepare a certain concentration, and add an appropriate amount of Low-molecular alcohol, adjust the pH value of the solution, stir and mix thoroughly, and establish a two-phase aqueous system.

[0030] (3) Two-phase extraction combined with microwave extraction: add Bletilla striata pow...

Embodiment 1

[0046] Put the tuber root of Bletilla striata at 60°C-80°C and dry it to constant weight. The dry tuber root is pulverized by a high-speed tissue grinder and sieved to obtain Bletilla striata powder of 20 mesh to 80 mesh; according to the design in Table 1, weigh a certain amount of Sodium carbonate and a certain volume of absolute ethanol, first add ultrapure water and stir to dissolve the inorganic salts, then add absolute ethanol, and finally add water to a final volume of 1000ml, stir well, and adjust the pH of the two-phase solution to 7.5. Weigh a certain amount of Bletilla striata powder, add it to the two-phase aqueous system, stir continuously, microwave at 400W, 50°C for 120 minutes to fully separate the Bletilla striata polysaccharide, let it stand for 60 minutes, and centrifuge at 10,000rpm for 30 minutes to separate the Bletilla striata polysaccharide For the extract phase, the extract phase is subjected to ultrafiltration and desalination with an ultrafiltration m...

Embodiment 2

[0050] Put the tuber root of Bletilla striata at 60°C-80°C and dry it to constant weight. The dry tuber root is pulverized by a high-speed tissue grinder and sieved to obtain Bletilla striata powder of 20 mesh to 80 mesh; according to the design in Table 1, weigh a certain amount of Disodium hydrogen phosphate and a certain volume of absolute ethanol, first add ultrapure water and stir to dissolve the inorganic salts, then add absolute ethanol, and finally add water to a final volume of 1000ml, stir well, and adjust the pH of the two-phase solution to 7.5. Weigh a certain amount of Bletilla striata powder, add it to the two-phase aqueous system, stir continuously, microwave at 400W, 50°C for 120 minutes to fully separate the Bletilla striata polysaccharide, let it stand for 60 minutes, and centrifuge at 10,000rpm for 30 minutes to separate the Bletilla striata polysaccharide For the extract phase, the extract phase is subjected to ultrafiltration and desalination with an ultraf...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a preparation method of bletilla striata polysaccharide. The preparation method comprises the following steps: drying bletilla striata tuberous roots, crushing and sieving; adding the bletilla striata powder into the prepared low-molecular-weight alcohol/salt aqueous two-phase system solution, performing microwave-assisted extraction, performing high-speed centrifugal separation to obtain a lower phase, performing ultrafiltration, concentration and desalination, and performing freeze drying to obtain bletillastriata polysaccharide. Compared with the prior art, the method can effectively shorten the extraction time, improve the extraction purity and reduce the extraction steps, and is simple and convenientto operate and easy for industrial scale preparation. The prepared bletilla striata polysaccharide is complete in structure, high in polysaccharide yield, high in protein removal rate, low in toxicity and safe, can prevent skin barrier damage and promote the repair process of the skin barrier damage, and has a good anti-allergic effect on sensitive skin with abnormal skin barrier functions.

Description

【Technical field】 [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of bletilla striata polysaccharide. 【Background technique】 [0002] Bletilla striata is the dry tuber of Bletilla striata (Thunb.) Reichb.f., a perennial herbaceous plant in the family Orchidaceae. It is commonly found in shaded grass or wetlands under forests. It is named because its roots are white and grows continuously. It is distributed all over my country . "Chinese Pharmacopoeia" records that the medicinal part of Bletilla striata is tuber, and its main medicinal ingredient is Bletilla striata polysaccharide, also known as Bletilla striata gum and Bletilla striata mannan. Studies have shown that the content of dry bulb tissue is about 40% to 50%. These polysaccharides are mainly composed of β-1,4-mannose, β-1,4-glucose and β-1,6-glucose residues. Bletilla striata polysaccharides mainly have a large molecular weight span, ranging from tens of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0087C08B37/0003
Inventor 郑雄健吴震杨煌建谢振荣
Owner 澜海(福建)生物科技有限公司
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