138 results about "Spotted deer" patented technology

The invention discloses an ecological breeding method for sika deer and belongs to the technical field of artificial domesticating of the sika deer. The method comprises the steps of breeding, juvenile deer managing, adult deer managing and the like. In the technical scheme, the feeding formula proportion and feeding amount are adjusted and controlled in strict accordance with the season replacement and different physiological demands of the sika deer in a growth period, a parturition period and a cartialgenous producing period, so an effective supply effect of foodstuff is achieved; concentrate and coarse materials are mixed in the foodstuff for breeding, green grass in nearby mountains are collected as foodstuff, multiple plants favorable for the growth of the sika deer are blended in the green grass, multiple original ecological nutrition components are absorbed, balanced nutrition supply is achieved, and the effect of improving the cartialgenous yield is achieved. The healthy growth of the sika deer is facilitated by formulas such as bean pulp and wheat bran, the digestion and absorption are promoted, even the effect of enhancing the disease resistance is achieved and the bred sika deer barely get ill.

The invention provides a method for detecting deer-derived ingredients in glue type traditional Chinese medicines and products thereof. The method provided by the invention comprises the step of detecting by using the difference between the specific nucleotide sequence or amino acid sequence of a genome of a red deer and the specific nucleotide sequence or amino acid sequence of a genome of a spotted deer, wherein the specific amino acid sequence is shown in SEQ ID No. 1. By adopting the method provided by the invention, whether the glue type traditional Chinese medicines contain the deer-derived ingredients or not can be detected rapidly, so as to distinguish the authenticity; although polypeptides of collagen in the glue type traditional Chinese medicines are subjected to certain hydrolysis and destruction, the deer-derived ingredients in the glue type traditional Chinese medicines and products thereof can be identified on the basis that differential polypeptides in a main ingredient, namely collagen, of the glue type traditional Chinese medicines are high in content and little in damaged extent.

The invention relates to deer feed, which is formed by straw raw materials including corns, wheat bran and the like, Chinese herbal medicine raw materials, vitamins, mineral nutrient elements, amino acid and other ingredients, and has the effects of being green, environment-friendly and free of pollution, effectively balancing various nutrients in compound feeds and improving growth speed, feed utilizing rate and production performance.

The invention discloses a full-fare particle fodder and fodder manufacturing method to improve the quantity and quality of pilose deer horn of horse deer and sika deer, which comprises the following parts: maize, soya bean waste or soya bean cake, cotton seed waste or cotton-seed cake, maize germ waste or maize germ cake, fermented maize spirit glutinour rice (DDGS), fermented beer glutinour rice, wheat bran, maize albumen powder as concentrate, bulking aquacare, mineral and vitamin additive, stone powder, magnesium oxide, calcium hydrophosphate, calcium carbonate, table salt, zeolite powder, molasses as additive fodder, wherein the content of dried material is 85%-90%; the crude protein content is 16%-20%, which contains plenty of amino acid, calcium, phosphor, vitamin and trace element to satisfy the need of horse deer and sika deer during pilose deer horn growing period. The invention improves the utility of fodder by 15-20% and productivity over 30%, which is fit for using in the deer culturing place and fodder manufacturing factory.

The invention discloses a multiplex-polymerase chain reaction (PCR) kit for rapidly detecting a deer product, which is used for rapidly and accurately distinguishing the deer product. The invention also discloses the preparation method of the kit. A multiplex-PCR technology is adopted to amplify the specific molecular fragments of mitochondrial DNAs of sika deer, red deer, Tarim red deer, wapiti, reindeer, pig, cow, sheep and chicken, so that the kit is prepared and used for detecting a detected sample contains the components of the animal components and detecting a mixed sample; and the kit and the method have the characteristics that is operation is simple, sensitivity is high and speed is rapid.

The invention belongs to the technical field of biology, and particularly relates to a marker polypeptide for identifying pilose antler and antler of sika deer or red deer and application thereof. The marker polypeptide provided by the invention specifically comprises: a peptide fragment 1 which is as shown in SEQ ID No. 1: KAPQVSTPTLVELSR; and a peptide fragment 2 which is as shown in SEQ ID No. 2: HHGGEFTVVLQADFQK. The marker polypeptide and the detection method provided by the invention provide reference for searching marker peptide fragments in the related species lacking in the database. The marker polypeptide provided by the invention has excellent specificity and stability for pilose antler and antler of sika deer or red deer, is high in specificity, can be used for identifying pilose antler and antler medicinal materials, decoction pieces thereof and Chinese patent medicines fed by using raw powder, and has a good application prospect.

The invention relates to oral thick paste for enhancing immunity, delaying ageing and improving male renal functions. The oral thick paste is prepared from the following raw materials in parts by weight: 20 to 25 parts of honey, 5 to 16 parts of cultivated sika deer meat, 3 to 8 parts of cultivated sika deer tendons, 2 to 6 parts of cultivated sika deer penis, 5 to 15 parts of maca powder, 6 to 12parts of rhizoma polygonati, 8 to 14 parts of fructus lycii, 12 to 20 parts of rhizoma dioscoreae, 3 to 9 parts of fructus alpiniae oxyphyllae, 4 to 8 parts of fructus rubi and 9 to 17 parts of poriacocos. In addition, the invention further relates to a preparation method of the oral thick paste.

The invention relates to the use of fresh pilose antler freeze-dried powder in preparing medicament for treating stomach ache, wherein the medicament comprises (by weight ratio) astragalus root 200-400, root of herbaceous peony 50-200, corydalis ambigua 100-150, bitter orange 100-150, fresh sika deer antler 200-300, bee glue 100-150, and licorice root 50-100.

The invention discloses a molecular marker for identifying antler varieties. The molecular marker comprises 16 SNPs of which the sequences are shown as SEQ ID NO.1-SEQ ID NO.16 respectively. The invention further discloses a method for identifying the antler varieties as well as application of the molecular marker and the method. The invention finds the molecular marker shown as SEQ ID NO. 1-SEQ ID NO. 16 for the first time; the molecular marker can well distinguish cervus elaphus antler, cervus nippon antler and hybrid deer antler, has the success rate of 95% or above during identification of the hybrid deer antler within five generations, and is high in reliability; in order to guarantee legal rights of consumers, the most economical and effective novel molecular identification way is provided; the molecular marker can also be applied to molecular marker-assisted selective breeding and variety identification of hybrid deer.

The invention relates to a preparing method of flavored sika deer sausage, which is characterized by comprising the following steps of preprocessing sika deer fresh meat raw materials, mincing, seasoning ingredients, vaccum penetrating, pickling, cutting, selecting, firing, washing, filling, vacuum packaging, sterilizing, and finishing. According to the preparing method, the sika deer meat is selected to be processed, so that the sika deer sausage has unique taste, and is fresh and chewy. The sausage is processed by sika deer meat so as to have the functions of increasing immunity, supplementing middle qi, strengthening the five internal organs, resisting rheumatism, resisting cancer, preventing and curing cardiovascular diseases and reducing cholesterol.

The invention relates to the field of sika deer feeding, and discloses a feed for feeding a sika deer. The feed comprises two parts of a concentrated feed and a coarse feed, wherein the concentrated feed is prepared from the following components: sorghum, corn, rapeseed meal, soya bean meal, bean cakes, wheat bran, peanut, sesame, pumpkin, rice bran, carrot, amorpha fruticosa leaf powder, amorpha fruticose seeds, fish meal, bone meal, vegetable juice, yeast, multivitamin and salt; the concentrated feed contains a lot of calcium and vitamin D, and can be fully absorbed by the sika deer; rickets of born cubs can be prevented; the concentrated feed contains a ferulic acid and phytosterol, so that an intestinal tract disease can be prevented; and the sika deer can be delicate in meat, much in lean meat, few in connective tissues and good in taste after being fed by the concentrated feed; and the coarse feed comprises cornstalks, soybean stalks, dry alfalfa, dry sudangrass, dry pennisetum hydridum and lespedeza stalks; the coarse feed contains abundant crude protein and cellulose, so that the growth speed of the sika deer can be improved and the weight of the born cubs is improved.

The invention discloses a PCR kit capable of rapidly detecting cervus derived products, which is used for identifying deer products rapidly and accurately. A kit is prepared by utilizing the PCR technology to amplify specific molecule fragments of each mitochondrial deoxyribonucleic acid (DNA) of sika deer, red deer, cervus elaphus yarkandensis, cervus elaphus and reindeer, is used for detecting whether a detected sample contains constituents of the deer, and can detecting mixed samples. The PCR kit capable of rapidly detecting the cervus derived products and a preparation method have the advantages of simple structure, high sensitivity, and fast speed.

The invention discloses an extracting method for deerskin collagens. The extracting method comprises the following steps: dehairing the skin of a sika deer or the skin of a deer skin, cutting into small pieces, disintegrating by a high-speed tissue disintegrator, and removing liposoluble components by a supercritical CO2 extracting method to obtain the fat-removed deer skin; adding a NaC1 solution in the fat-removed deer skin, continuously pumping feed liquid into a high-voltage pulse extraction device by a liquid pump to obtain an impurity-removing protein precipitation, washing the protein precipitation with distilled water, centrifuging, removing a water layer to obtain a desalted precipitation, adding an organic acid solution in the desalted precipitation, adding a protease, continuously pumping feed liquid into the high-voltage pulse extraction device by the liquid pump, continuously collecting an extracting solution, centrifuging, concentrating a supernatant to a certain volume, and performing freeze drying to obtain a white solid. The extracting method has the advantages that thermosensitive components are invariable, the extraction is continuous and fast, the technology is advanced, and environment protection and high efficiency are achieved.

The invention provides a method for culturing ruminal epithelial cells of sika deer, relates to the field of isolation and culture of cells and aims to solve the problems of low purity, low vitality,low success rate, high probability of contamination and the like of culture of the ruminal epithelial cells of the sika deer. The method comprises the following steps: ruminal tissue of the sika deeris taken and washed with PBS containing antibiotics; epithelial tissue is taken and cut into pieces, and the pieces are added to a DMEM / F12 culture solution containing FBS (fetal bovine serum), sodiumselenite and the antibiotics for adherent culture; the pieces are added to the DMEM / F12 culture solution containing the FBS, sodium selenite and the antibiotics for subculture. The ruminal epithelialcells of the sika deer can be separated out successfully for the first time with the method, purity and activity are higher, steady passage can be realized, and the problem of contamination caused byvarious microorganisms in the rumen is solved. The method can be applied to research in growth and development, physiological functions, nutrition metabolism and other aspects of ruminal epithelium of the sika deer.

The invention discloses a novel extracting method of deer spleen extract and an application of deer spleen extract in immunity enhancement drugs. A traditional deer spleen extracting technology is improved, and centrifugation sediments are further extracted by a complex enzyme. Both polypeptide content and nucleotide content are improved by 30%. Moreover, the inosine ingredient occupies 8% of total nucleotide content in the extract, and the inosine content in the extract is improved. Meanwhile, the activity of the extract obtained by the method is improved by 20% than that of transfer factors extracted by the original technology for increasing the white blood cells.

The invention provides a formula and preparation method of microcapsules prepared from deer horns and pilose antlers of sika deers. The preparation method comprises the following steps of performing cleaning; performing drying; performing coarse crushing; performing superfine crushing; compounding core material mixed liquor; compounding wall material mixed liquor; performing embedding; performing curing; performing cleaning; performing drying; and performing packaging. The formula of the microcapsules comprises the components of the deer horns and the pilose antlers of the sika deers, sodium alginate, sucrose fatty acid ester, sodium tripolyphosphate, carboxymethyl chitosan and calcium chloride. According to the formula disclosed by the invention, the dispersibility, the stability, the safety, the bacterium resistance and the oxidation resistance of micro powder made from the deer horns and the pilose antlers of the sika deers can be improved, and besides, the mechanical strength of the microcapsules prepared from the deer horns and the pilose antlers of the sika deers is strengthened. According to the formula disclosed by the invention, the deer horns and the pilose antlers of the sika deers are in superfine crushing through a technique and are prepared into the microcapsules; and therefore, sufficient absorption of nutrient components of the microcapsules by human bodies can be improved, bad smell of the deer horns and the pilose antlers can also be concealed, the stability of products is improved, and slow release of the nutrient components is controlled.

The invention discloses an STR molecule marker based on sika deer whole genome developing and application of the STR molecule marker. Based on a sika deer whole genome sequence, screening for STR loci is conducted, primer is designed and the application effect is verified, and the sika deer STR molecule marker with high amplification efficiency and the high recognition rate is finally obtained and used for amplifying primer sequences of the STR molecule marker as shown in SEQ ID NO:1-62. According to the STR molecule marker based on sika deer whole genome developing and application of the STR molecule marker, a four-base repeated unit is contained in the sika deer STR locus, compared with routine two-base to three-base repeated units, the distinguishing degree is higher and more stable, gene type judging is easier, and the convenient and efficient molecule marker can be supplied for genetic research on sika deer groups.

The invention relates to oral thick paste capable of enhancing immunity, beautifying face and nourishing female renal function. The oral thick paste contains the following raw materials in parts by weight: 10-30 parts of honey, 1-4 parts of cultivated sika deer blood, 1-5 parts of cultivated sika deer heart, 2-8 parts of donkey-hide gelatin, 8-15 parts of barbary wolfberry fruit, 10-20 parts of longan pulp, 12-18 parts of poria, 7-15 parts of pitted Chinese dates, 9-18 parts of Chinese yam and 12-22 parts of black beans. Besides, the invention further relates to a preparation method of the oral thick paste.

The invention provides the novel route of administration and preparing process for Chinese medicinal injection for treating nervous prostration, loss of appetite, undernourishment, coronary disease, angina pectoris and arrhythmia. The injection is prepared from sika deer horns as the raw material, and can be made into the dosage forms of concentrated solution for injection, freeze-dried powder, germ-free powder, 5% glucose injection, and 9% sodium chloride injection.

The present invention discloses a deer placenta and donkey-hide gelatin solid beverage and a production technology thereof, which relates a combination method of medicinal and edible traditional Chinese medicine materials used in health-care foods and extraction and preparation methods thereof. The solid beverage is prepared from the following raw materials in parts by weight: 10-50 parts of donkey-hide gelatin, 5-15 parts of cervus nippon hide, 5-20 parts of astragalus membranaceus, 5-15 parts of codonopsis pilosula, 5-10 parts of radix rehmanniae praeparata, 5 parts of angelica sinensis, 15-20 parts of red jujubes, 2.5-10 parts of panax ginseng, 5-6 parts of ziziphus jujuba var.spinosa, 1 part of soy isoflavone, and 9-20 parts of deer placenta or dehydrated deer placenta. The beneficial effects of the solid beverage are as follows: the beverage has functions of preventing aging, beautifying features and nourishing skin, supplementing qi and nourishing blood, preventing fatigue, and improving immunity, and is convenient for consumption.

The invention relates to application of a sika deer spleen extract to antioxidant medicines. Animal experiments show that the sika deer spleen extract prepared by the process provided by the invention can reduce peroxides and protein carbonyls in serum of an ethanol-damaged mouse model, increase the content of reductive glutathione and enhance the activity of SOD in the serum, has an antioxidant effect, and can be used for preparing the antioxidant medicines and health-care food.

The invention discloses a method for identifying sika deer, red deer or other hybridized deer on basis of COI and SRY sequences. The invention provides the application of a substance for detecting a target site in gene sequences corresponding to a COI gene and an SRY gene in a genome of a to-be-detected sample as well as target nucleotide of the site to preparation of products for detecting whether the to-be-detected sample is sika deer, red deer or other hybridized deer. Two multiple PCR systems are established by utilizing the SRY gene of Y chromosome as an identification mark of a male parent and combining a female parent mark gene COI on the basis of the COI and SRY gene sequences and are used for identifying male and female parent sources of cartialgenous samples, the identification method of the hybridized cartialgenous is initially established, and male and female parent identification of the hybridized deer can be conducted effectively. The method has actual application value on identification of the hybridized cartialgenous, and provides an effective identification method for subsequent quality evaluation of the hybridized cartialgenous.

The invention discloses a sika deer mucosal disease homologous inactivated vaccine and a preparation method thereof. The preparation method comprises the following steps: performing inoculated culture on deer source BVDV (bovine viral diarrhea / virus) to the third to sixth generations in stable cell flasks with MDBK (madin-darby bovine kidney) cells fully spread in a single layer, selecting cell flasks which are cultured for 72-144 hours and generate a cytopathic effect (CPE), repeatedly freezing and thawing after pathological change, collecting viral liquid, adding formaldehyde into the viral liquid for inactivating, mixing the inactivated viral liquid with an oil agent consisting of white oil and sorbitan, and adding aluminum stearate to obtain the sika deer mucosal disease homologous inactivated vaccine.

The invention provides a sika deer velvet nucleic acid series extract product and process for preparation comprising, proceeding micro-encapsulation embedding process to nucleic acid through double agglomeration method microcapsule granulating technology, thus obtaining sika deer velvet nucleic acid capsule, charging milk product into belt stirrer for preheating, adding cane sugar, stabilizer for hydration treatment, stirring to fully dissolve the materials, hydrating the colloid completely, subjecting the sika deer velvet nucleic acid microcapsule to disinfection and cooling down, charging in and mixing evenly, circulation stirring, cooling down and fixing volume to required production quantity. The invention can be applied to increase the technological content and added value for the dairy product manufacture.

The invention relates to a spotted deer antler liquid and a preparation method thereof, belonging to the technical field of the deep processing of deer antler products. The invention aims to solve theproblems that the prior deer antler processing and application methods cause the waste and damage of most nutrient content of the deer antler or deer antler prepared by the prior process can not be fully utilized by human bodies, and the like. The spotted deer antler liquid has obvious effect on improving the symptoms of impotence, involuntary emission, uterine cold sterility, emaciation, neurolysis, intolerance of cold, dizziness and tinnitus, cold and pain along spinal column, bones and muscles atrophy, uterine bleeding and vaginal discharge, unhealing Yin jaundice and the like.

The invention discloses sika-deer Wujin oral liquid, which comprises, by weight, 20-30 parts of sika deer meat, 20-30 parts of black soybeans, 10-20 parts of glossy ganoderma, 10-25 parts of Chinese pond turtle, 20-30 parts of soybeans, 30-50 parts of dried rehmannia root, 200-300 parts of prepared rehmannia root, 40-50 parts of smoked plum, 10-30 parts of lemon, 20-30 parts of mulberry, 15-30 parts of poria cocos, 10-20 parts of liquorice, 30-60 parts of cornus officinalis, 20-50 parts of rhizoma alismatis, 50-80 parts of black rice, 20-50 parts of Danshen root, 30-60 parts of hawthorn, 20-30 parts of medicated leaven, 20-50 parts of cinnamon, 30-60 parts of Dioscorea opposita, and 50-500 parts of red dates. The invention further provides a method of preparing the sika-deer Wujin oral liquid. Orally taking the sika-deer Wujin oral liquid can regulate human immunity, regulate the five internal organs, enhance memory, relieve sores and aches of shin, waist and leg, remove fatigue, prevent diseases, build body, achieve nourishing and beauty care, and prolong life.

The invention relates to a preparation method of sika deer skin collagen and collagen peptide. According to the method, the extraction rate is mainly taken as an index, the optimal extraction method is selected through different extraction methods to extract the collagen, namely, the collagen in the sika deer skin is coarsely extracted by adopting an ultrasonic-assisted enzyme extraction mode, so that the collagen with relatively high extraction rate is obtained, and the collagen peptide is prepared from the extracted collagen by selecting different enzymolysis. In the aspect of physicochemical properties, the collagen peptide prepared from the compound protease and the papain has better physicochemical properties, and in the aspect of functional properties, the collagen peptide prepared from the papain shows better oxidation resistance.

The invention discloses a deer bone marrow-containing health care product for strengthening bone. The deer bone marrow-containing health care product is prepared from the following raw materials in parts by mass: 1-10 parts of deer bone marrow powder, 1-10 parts of deer meat powder, 1-5 parts of collagen powder and 0.1-2 parts of vitamin D3. The deer bone marrow powder and the deer meat powder are obtained by performing drying and ultramicro crushing treatment on the deer bone marrow or the deer meat of fresh red deer or sika deer; the deer bone marrow contains various nutrient substances such as rich amino acids, various protein polypeptides, a plurality of mineral substance and vitamins; the deer bone marrow powder also contains rich bone collagen and chondroitin and is an important source of active calcium; the deer bone marrow is capable of strengthening bone and supplementing calcium, and combined with the collagen in the deer bone marrow-containing health care product and deposited on the bone; in addition to the vitamin D3, the absorption and utilization of the supplied calcium and the supplied collagen can be mutually promoted, and the bone nutrition can be supplied comprehensively, so that the nutritional ingredients of the bone can be balanced in proportion, and the whole organism is firm, flexible and elastic; besides, the deer bone marrow further contains active hematopoietic factor so as to enrich the blood and tonify deficiency.

The invention provides a molecular marker for identifying subspecies of northern sea track of sika deer, an identification method and application, and aims at accurately identifying the subspecies ofthe northern sea track of the sika deer. Four SNP sites S-1 to S-4 of the molecular marker are respectively shown in the subspecies of the northern sea track of the sika deer, and are respectively shown in non-subspecies of the northern sea track of the sika deer. The invention also provides a method for identifying the subspecies of the northern sea track of the sika deer. The method comprises the following steps of: performing PCR amplification and sequencing on any one or more pairs of primer pairs 1 to 4, and identifying whether a sample to be tested is the subspecies of the northern sea track of the sika deer according to SNP marker site bases. The four SNP sites are positioned on four gene segments, and have the characteristics of high specificity and high stability. The identification detection accuracy is 100 percent. The invention provides a stable and reliable molecular detection method for identifying subspecies of sika deer resources. The molecular detection method has important theoretical and application values in the aspects of DNA fingerprint drawing of the sika deer, wild subspecies protection management and the like.