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94 results about "Spotted deer" patented technology

The sika deer (Cervus nippon) also known as the spotted deer or the Japanese deer, is a species of deer native to much of East Asia, and introduced to various other parts of the world. Previously found from northern Vietnam in the south to the Russian Far East in the north, it is now uncommon in these areas, excluding Japan, where the species is overabundant.

Construction method and application of sika deer immortalized renal epithelial cell line

The invention relates to the technical field of cell engineering, and particularly discloses a construction method and application of a sika deer immortalized renal epithelial cell line, and the construction method comprises the following steps: taking renal epithelial primary cells from healthy sika deer renal cortex tissues to obtain primary renal epithelial cells; treating the primary renal epithelial cells with trypsin to obtain a cell suspension; inoculating the cell suspension into a complete culture medium, and transfecting with lentivirus loaded with SV40 large T antigen genes to obtain transfected cells; 3 [mu] g / mL puromycin is applied to the transfected cells for selective culture, so that non-transfected cells are eliminated, and drug-resistant cells are obtained; carrying out continuous passage on the drug-resistant cells for at least 30 generations to obtain the immortalized renal epithelial cell line of the sika deer; according to the invention, through a specific action mechanism (inhibiting a p53 / pRb pathway and blocking cell cycle exit) of the SV40 large T antigen, a proliferation limit caused by inhibition of telomerase activity of primary cells of the cervidae animals is overcome, and a cell resource library capable of realizing continuous passage is established.
Owner:JILIN AGRICULTURAL UNIV

Antibacterial peptide CnCATH as well as coding gene and application thereof

The invention relates to an antibacterial peptide CnCATH as well as a coding gene and application thereof, and belongs to the technical field of biomedicine. The novel antibacterial peptide is extracted from sika deer bone marrow, and the amino acid sequence is as shown in SEQ ID NO. 2. The antibacterial peptide shows strong antibacterial activity on various microorganisms including gram-negative bacteria, gram-positive bacteria and fungi, the sterilization speed of the antibacterial peptide is obviously improved compared with that of traditional antibiotics, the bacterial strain is not prone to drug resistance, endotoxin can be further neutralized, and the concentration of the endotoxin can be further reduced. The novel sika deer-derived antibacterial peptide provided by the invention has the advantages of small molecular weight, simple artificial synthesis, low drug resistance tendency and high broad-spectrum bactericidal activity, and provides a new strategy for microbial anti-infection treatment and prevention and treatment of diseases caused by endotoxin.
Owner:SUZHOU NINTH PEOPLES HOSPITAL (SUZHOU WUJIANG DISTRICT FIRST PEOPLES HOSPITAL)

Sika deer antler weight whole genome SNP site combination, probe, chip and application thereof

The invention provides a sika deer antler heavy whole genome SNP site combination, a probe, a chip and application thereof, and relates to the technical field of molecular breeding. The sika deer antler weight whole genome SNP locus combination comprises 6302 SNP loci, the number of the loci is sufficient, the loci can fully cover a main genetic area affecting deer antler weight, and the accuracy, stability and repeatability of genetic evaluation are effectively improved; the Northeast sika deer capture efficiency evaluation is carried out on a Northeast sika deer sample based on a probe designed on 6302 SNP loci, the capture proportion is kept at 98.60%-99.80%, the overall capture efficiency is high, the stability is good, and the Northeast sika deer capture efficiency evaluation method has good application value.
Owner:HEILONGJIANG ACAD OF LAND RECLAMATION SCI

Method for extracting sika deer blood polypeptide through microwave-assisted enzyme method and application of sika deer blood polypeptide in anti-fatigue food

The invention relates to an enzymolysis technology and a microwave-assisted extraction technology in a bioengineering technology, in particular to a method for extracting sika deer blood polypeptide through a microwave-assisted enzyme method and application of the sika deer blood polypeptide in anti-fatigue food. Treating the deer blood by using a composite anticoagulant containing sodium citrate, centrifuging, and freezing and thawing to obtain a lysate; carrying out microwave-assisted malic acid acidification, carrying out staged enzymolysis by using a compound enzyme composed of bromelain according to a ratio of 4: 3: 2, and introducing carbon dioxide to control the pH value; performing enzyme deactivation and centrifugal ultrafiltration to obtain a crude product, purifying the crude product through an ion exchange column and a gel column, and performing Maillard reaction modification with chitosan oligosaccharide; and finally preparing the anti-fatigue food with maltodextrin as an auxiliary material. According to the method, the yield of the sika deer blood polypeptide is greatly improved, the proportion of high-activity small molecule peptide is extremely high, the stability and bioavailability of the modified polypeptide are remarkably improved, and the bitter taste is reduced; the efficacy of the prepared anti-fatigue food is superior to that of similar products, and high-value utilization of deer blood resources is promoted.
Owner:JILIN ZHENGHE BIOTECHNOLOGY CO LTD

A cervus nippon and cervus canadensis off-season antler absorbable sustained-release control agent and a preparation method thereof

PendingCN122124066AOrganic active ingredientsAerosol deliveryPhysiologyDextranomer hyaluronic acid
This invention relates to an absorbable, slow-release regulator for off-season antler growth in sika deer and red deer, and its preparation method, belonging to the field of off-season antler growth regulators. The regulator comprises 330 mg–360 mg medroxyprogesterone acetate (MPA), 50 mg–55 mg dextran sulfate (DS), and 200 mg–220 mg sodium hyaluronate (HA), diluted with water for injection to a final volume of 1.0 mL. The molecular weight of medroxyprogesterone acetate (MPA) is 386.52 kDa; the molecular weight of dextran sulfate (DS) is 36–50 kDa; and the molecular weight of sodium hyaluronate (HA) is 20–400 kDa. This invention utilizes exogenous progesterone to induce off-season antler growth in male deer through hormonal regulation. The use of absorbable materials is environmentally friendly and harmless to the body, allowing for natural absorption and normal excretion through the body's metabolic processes. A single injection is sufficient to provide efficacy for over 35 days. Using progesterone to regulate off-season antler growth is low-cost and has a high success rate.
Owner:HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY

Automatic feeding device for breeding sika deer and using method thereof

The invention discloses an automatic feeding device for sika deer breeding and a using method thereof.The automatic feeding device comprises an inverted-T-shaped shell, feeding grooves are formed in the tops of the two sides of the shell in an inwards-concave mode respectively, flattening assemblies are movably arranged in the feeding grooves respectively, cover plates are arranged at top openings of the feeding grooves, and the opposite ends of the cover plates are fixedly connected with opening and closing assemblies respectively; the opening and closing assembly and the flattening assembly are arranged on the other two sides of the shell correspondingly. A material storage cavity is formed in the bottom in the shell in a hollow mode, a stirring mechanism is movably arranged in the material storage cavity, the bottom end of the stirring mechanism is in transmission connection with the end of an auger, the auger is rotationally arranged in discharging ports, the discharging ports are formed in the two sides of the bottom of the material storage cavity in a penetrating mode, and the ends, away from the material storage cavity, of the discharging ports communicate with the feeding grooves; the top of the stirring mechanism is movably arranged in the transmission cavity, the transmission cavity is hollow and arranged at the top in the shell, the top end of the stirring mechanism is fixedly connected with an output shaft of a second waterproof motor, and the second waterproof motor is fixedly installed on the outer wall of the top end of the shell.
Owner:HUNAN JINFENG AGRI & ANIMAL HUSBANDRY DEV CO LTD

Doll (Chimelong Baby Bottle Series Sika Deer)

ActiveCN309499592SSpotted deerTesting Methods
1. The name of the product of this design: Doll (Chimelong Baby Bottle Series Sika Deer). 2. Purpose of this design product: to be used as an entertainment toy. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture.
Owner:GUANGDONG CHANGLONG CULTURAL IND CO LTD

Preparation method and verification method of deer skin collagen peptide with skin repairing and immunoregulation effects

The invention relates to a preparation method and a verification method of deer skin collagen peptide with skin repairing and immunoregulation effects, and belongs to the technical field of biological medicines. The technical problems that the technology for extracting the deer skin collagen peptide from the deer skin of the sika deer is tedious, and whether the prepared deer skin collagen peptide has skin repairing and immunoregulation effects or not are solved. The preparation method of the deer skin collagen peptide with the skin repairing and immunoregulation effects comprises the following steps: cutting deer skin into small pieces, and preparing the deer skin collagen peptide with the molecular weight of less than 10kDa by adopting a double-step enzymolysis method. And a verification experiment of an immunosuppressive mouse is constructed through cyclophosphamide induction. According to the invention, the extraction process is greatly simplified, and a double-step enzymolysis method is adopted, so that the deer skin collagen peptide subjected to enzymolysis is small-molecule peptide, has strong antioxidant capacity, can repair light-damaged cells and promote skin cell growth, and is good in safety; by injecting the deer skin collagen peptide, the immune function of the mouse is improved, and damage caused by mouse cells is relieved.
Owner:长春科技学院

A feces cleaning device for sika deer breeding

The utility model discloses a feces cleaning device for sika deer breeding, comprising an anti-odor device, a cleaning device body, an opening and closing door and a universal wheel. The utility model sets a first screen plate, a sliding groove and a connecting rod transmission mechanism, so that the first screen plate is slidably installed with the cleaning device body through a sliding block and a sliding groove, and then the connecting rod transmission mechanism can convert the circular motion into a linear reciprocating motion, and then the connecting rod transmission mechanism drives the first screen plate to move left and right. When feces are put into the top of the first screen plate inside the cleaning device body, the feces will continuously shake through the movement of the first screen plate, so that the stones in the feces can be quickly screened out, and the problem that the screen plate in the above-mentioned device adopts a fixed installation method to screen the stones in the feces is solved, which leads to the feces being mixed with stones to generate accumulation when too much feces is put in, and then only a small amount of feces can be put in when the feces is put in, and the efficiency is too low.
Owner:湖北省尊禧鹿业有限公司

Composition for promoting proliferation of mesenchymal stem cells and / or relieving damage of spermatogonium as well as preparation method and application of composition

The invention provides a composition for promoting proliferation of mesenchymal stem cells and / or relieving spermatogonial cell injury as well as a preparation method and application of the composition, and belongs to the technical field of traditional Chinese medicine compositions. The composition is prepared from the following raw materials in parts by weight: 10-20 parts of ginseng, 5-15 parts of maca, 5-10 parts of oyster peptide, 5-15 parts of sika deer penis, 3-8 parts of sika deer blood, 3-8 parts of fresh ginger, 5-8 parts of herba cistanche, 4-10 parts of sea cucumber peptide, 4-8 parts of rhizoma polygonati, 5-10 parts of burdock root, 2-5 parts of fructus lycii powder and 1-3 parts of folium cortex eucommiae. The composition is prepared into a traditional Chinese medicine preparation by adopting a superfine grinding process, and the traditional Chinese medicine preparation has the effects of promoting proliferation of mesenchymal stem cells (MSCs), repairing spermatogonial cell injury caused by oxidative stress (such as chemotherapy and radiation) or aging (such as age-related), reducing the ROS level, improving spermatogenic dysfunction, assisting in treating male infertility and delaying senescence of a reproductive system and a whole body.
Owner:HUNAN YUANPIN CELL TECH CO LTD

Artificial lactation device for young sika deer

The utility model relates to the technical field of livestock breeding equipment, in particular to an artificial feeding device for young sika deer, and solves the problems that in the prior art, due to the fact that a feeding bottle frame is arranged in the horizontal direction, the transverse space is occupied, and the occupied area is large; and the device is not compact enough in a livestock farm with limited space, so that the applicability and the use convenience of the device are influenced. The device comprises a base, a center column and a mounting ring, the center column is vertically fixed to the top of the base, the mounting ring is in sliding fit with the center column through an inner ring and is supported by a connecting rod, a plurality of angle-adjustable bearing plates and feeding bottles are arranged on the outer ring of the mounting ring, and height adjustment is achieved through driving of a lifting assembly. By means of the surrounding type layout, the occupied transverse space is effectively reduced, the structural compactness is improved, meanwhile, the height and angle adjusting function is achieved, the breast-feeding requirements of young deer of different body types are met, operation convenience and equipment applicability are improved, and the actual requirements of modern farms for efficient and intensive equipment are met.
Owner:JILIN AGRICULTURAL UNIV

Book sticker (spotted deer)

ActiveCN309485930SSpotted deerZoology
1. The name of this design product: Book sticker (spotted deer). 2. Application of this design product: for book labeling. 3. The key design point of this design product lies in the combination of shape and pattern. 4. The picture or photo that best illustrates the design points: three-dimensional picture.
Owner:YIWU KEMO DAILY NECESSITIES CO LTD

Lyophilized sika deer antler powder tablet and preparation method thereof

The invention relates to a lyophilized sika deer antler powder tablet and a preparation method thereof. The main raw material of the pilose antler freeze-dried powder tablet is pilose antler, and the formula of the pilose antler freeze-dried powder tablet is as follows: each 100g of raw material contains 3-5g of medlar, 2-6g of angelica sinensis, 2-4g of maltodextrin, 1-4g of hard magnesium and the balance of pilose antler. The preparation method adopts a modern technology, is fine in process, optimizes parameters, improves the utilization rate of product components, is high in product quality, and has a greater health-care value on the precious traditional Chinese medicinal material, namely, the cornu cervi pantotrichum.
Owner:孙树成 +2

Method for extracting and separating cord blood and placenta stem cells of sika deer

The invention discloses a method for extracting and separating cord blood and placenta stem cells of sika deer, and belongs to the technical field of biomedicine. The problems that an existing stem cell extraction and separation method is low in efficiency, poor in cell activity, low in purity and large in pollution risk are solved. The method comprises the following steps: collecting cord blood and placenta after delivery of sika deer; diluting the cord blood, performing density gradient centrifugation, collecting the albuginea layer enriched with the stem cells, adding an erythrocyte lysate to remove residual erythrocytes, and centrifuging and collecting the stem cells; the method comprises the following steps: cutting placenta tissues into pieces, putting the placenta tissues into trypsin-EDTA digestive juice containing collagenase, carrying out ultrasonic-assisted digestion, and centrifuging to obtain stem cells enriched at the bottom of a centrifugal tube; and finally, resuspending the collected stem cells in a culture medium containing fetal calf serum and growth factors, adjusting the cell concentration, and inoculating into a cell culture bottle for culture. According to the method, the extraction efficiency and quality of the sika deer cord blood and placenta stem cells can be remarkably improved, and the activity and safety of the cells are ensured.
Owner:长春科技学院

Weighing equipment for sika deer breeding

The utility model discloses a sika deer breeding weighing device, which comprises a controller, a weighing plate, a mobile base and a guardrail, the weighing plate is electrically connected with the controller through a lead, the top of the mobile base is fixedly connected with the bottom of the weighing plate, the guardrail is fixedly arranged at the top of the mobile base, and the controller is electrically connected with the controller through a lead. And the top of the weighing plate is movably connected with a scraping plate, the bottom of the movable base is movably connected with a moving mechanism, and a transmission mechanism is arranged on the left side of the scraping plate. According to the utility model, through the arrangement of the scraping plate, a user can scrape residues at the top of the weighing plate by using the scraping plate, so that the problems that dirt and residues are accumulated at the top of the weighing plate after the device is used for many times, and the accuracy of weighing data is influenced by the existence of foreign substances are solved; and biological substances such as hair and saliva can be left in the weighing process of the sika deer, and the substances become transmission media of pathogens if not cleaned in time, so that the effect of auxiliary cleaning is achieved.
Owner:湖北省尊禧鹿业有限公司

Cornu cervi pantotrichum variety identification method based on multi-dimensional data fusion and artificial intelligence optimization algorithm

The invention relates to a pilose antler variety rapid identification method and system based on multi-dimensional data fusion and an artificial intelligence optimization algorithm, and the method comprises the steps: collecting multi-dimensional feature data of colors, textures, component contents and the like of pilose antler pieces of different varieties by using computer vision (CV), high performance liquid chromatography (HPLC) and a rapid meteorological electronic nose technology (UF-GC-E-nose); comprising sika antler, hairy antler, domesticated antler and camel antler, efficient, accurate and automatic variety identification and traceability are realized, the characteristic that the whole is superior to the sum of parts in a complex system is reflected, a good traceability result is generated, and a constructed intelligent algorithm based on multi-dimensional data fusion provides reference and data support for food variety identification and traceability.
Owner:NANJING UNIV OF TRADITIONAL CHINESE MEDICINE

Feed mixing and stirring mechanism for sika deer breeding

The feed mixing and stirring mechanism comprises a stirring tank, a stirring rod, a motor, a water spraying device, a feeding pipe and a discharging pipe, the bottom of the motor is fixedly connected with the top of the stirring tank, and the output end of the motor penetrates through the top of the inner wall of the stirring tank and is fixedly connected with the top of the stirring rod. By arranging the scraping strip plate, when feed adhered to the inner wall of the stirring tank is removed, the motor is started, and the output end of the motor drives the stirring rod to rotate, so that the bracket rod rotates and drives the scraping strip plate to scrape the feed adhered to the inner wall of the stirring tank, and the feed is discharged from the discharge pipe; the sika deer feed stirring device solves the problem that sika deer breeding health is affected due to the fact that feed cannot be completely discharged from a discharge port and can be adhered to the inner wall of a stirring tank and a stirring rod, if the feed is not treated in time, bacteria and molds are bred, deterioration occurs, and when new feed is stirred, the deteriorated feed can be doped into the new feed, and the effect of auxiliary cleaning is achieved.
Owner:湖北省尊禧鹿业有限公司

High-speed hair dryer (Sika deer)

1. The name of the product of this design: High-speed hair dryer (Sika deer). 2. Purpose of the product of this design: used for drying and styling hair. 3. The design key point of this appearance design product lies in the shape. 4. The picture or photo that best illustrates the design points: Stereoscopic diagram 1.
Owner:王保华

Anti-icing water drinking equipment for sika deer breeding

The utility model relates to the technical field of livestock breeding equipment, in particular to anti-freezing drinking equipment for sika deer breeding, and solves the problems that in the prior art, an effective anti-freezing or heating system is not arranged, the interiors of a drinking trough and a water pipe are easily frozen in a low-temperature environment in winter, water flow is unsmooth and even is completely frozen, and the service life of the drinking equipment is prolonged. The normal water drinking of the sika deer is seriously influenced. The equipment comprises a supporting table, two semicircular water troughs, a lifting frame, a heating structure, a flexible filter plate and a vertical lifting mechanism, the water troughs are symmetrically arranged on the two sides of the top of the supporting table, and the lifting frame is embedded in the water troughs and connected with the heating structure. According to the sika deer breeding device, the heating rods distributed in an annular array mode are arranged, automatic heating can be achieved in a low-temperature environment to prevent a water body from freezing, meanwhile, the lifting adjusting function is combined, the stability of drinking water supply in winter is guaranteed, the applicability and maintenance convenience of the device are improved, and the actual requirement for sika deer breeding in cold regions is effectively met.
Owner:JILIN AGRICULTURAL UNIV

Sika deer sperm sorting solution, sika deer Y sperm sorting kit based on sorting solution and application of sika deer Y sperm sorting kit

The invention provides a sika deer sperm sorting solution, a sika deer Y sperm sorting kit based on the sorting solution and application, and relates to the technical field of livestock breeding. The sika deer sperm sorting liquid comprises a sorting basic liquid and a Y sperm sorting functional component, wherein the Y sperm sorting functional component is prepared from rasimod and hyaluronic acid. Rescimod in the sika deer sperm sorting liquid I can be specifically combined with a sika deer X sperm TLR7 / TLR8 membrane receptor to inhibit the movement ability of the sika deer X sperm TLR7 / TLR8 membrane receptor, and a Y sperm is not affected due to lack of the receptor; on the basis, hyaluronic acid in the sorting liquid I and the sorting liquid II forms a physical barrier by increasing the viscosity of the sorting liquid, X sperms with limited movement and Y sperms with insufficient activity are effectively blocked, the Y sperms with high activity cross a barrier layer formed by the sorting liquid I and the sorting liquid II to enter a collecting liquid, then efficient sorting of the functional Y sperms of the sika deer is successfully achieved, and the sorting efficiency of the functional Y sperms of the sika deer is improved. The high-purity and high-activity Y sperms are enriched.
Owner:INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS

Character polypeptide for distinguishing and identifying antler medicinal material based on chemical marker, kit and identification method thereof

The invention belongs to the field of biological detection, and particularly relates to a marker polypeptide for distinguishing and identifying antler medicinal materials and preparations thereof based on chemical markers, a kit and an identification method of the marker polypeptide. According to the chemical marker adopted by the invention, chemical substances which are specific from a certain medicinal material (decoction piece) but not from other medicinal materials are searched as identification indexes and are used for true and false identification and quality control of the traditional Chinese medicine. The marker polypeptide is used as a chemical marker of the antler medicinal material, so that a plurality of species can be distinguished and identified. The used chemical marker-marker polypeptide is derived from the medicinal material, and identification of sika deer, red deer, dama dama and roe deer is realized.
Owner:SHANDONG INST FOR FOOD & DRUG CONTROL

Multifunctional composite sika deer grass-free feeding feed formula and preparation method thereof

The present invention discloses a multifunctional compound sika deer grass-free feeding feed formula and a preparation method thereof, and relates to the technical field of animal nutrition and feed science, the feed formula comprises: a basic energy raw material consisting of corn flour, soybean protein isolate and palm oil; the functional additive contains a trace element chelate, a plant extract and active peptide; the fiber substitute comprises inulin, pectin and sodium carboxymethyl cellulose. The basic energy raw material, the functional additive and the fiber substitute are scientifically proportioned, the feed is prepared through wet granulation and an antioxidant spraying process, the particle diameter of the feed is 3-5 mm, the water content is lower than 8%, the high-energy requirement of sika deer in a grass-free feeding mode can be met, the palatability and digestion and absorption performance are optimized, and the feed is suitable for large-scale popularization and application. Meanwhile, intestinal health is maintained, pilose antler growth is promoted, and the application value is remarkable.
Owner:JILIN AGRICULTURAL UNIV

Cervus cocktail with uric acid-lowering effect

This invention relates to the field of pharmaceutical and food technology, specifically to a deer tail wine and its uses, particularly its application in the preparation of drugs for lowering uric acid or enhancing immunity. The deer tail wine of this invention is prepared from the following raw materials: 1-20 parts of artificially bred sika deer tail, 1-20 parts of Eucommia ulmoides male flowers, 1-30 parts of raspberry, 10-50 parts of burdock root, and 10-30 parts of Lophatherum gracile. The deer tail wine is obtained by soaking the above raw materials in baijiu (Chinese white liquor) and filtering. This invention's deer tail wine adheres to the principles of using authentic medicinal materials and food from the same source. It selects five precious Chinese medicinal herbs—artificially bred sika deer tail, Eucommia ulmoides male flowers, raspberry, burdock root, and Lophatherum gracile—using baijiu as an adjuvant, and is produced through traditional soaking techniques and modern high-tech filtration processes. It not only has a significant effect on lowering blood uric acid but also regulates intestinal flora and enhances immunity.
Owner:HEALTH IND CO LTD OF KWEICHOW MAOTAI GRP

Beverage with effects of activating blood and warming body and preparation method thereof

The invention discloses a beverage with blood activating and body warming effects and a preparation method thereof. The beverage with the effects of activating blood and warming the body is prepared from the following raw materials in parts by weight: 5-10 parts of coffee beans, 4-8 parts of sika deer antler blood powder, 4-8 parts of pawpaw, 4-6 parts of agaricus blazei murill, 2-4 parts of angelica sinensis, 1-3 parts of ginseng, 1-3 parts of polygonatum kingianum, 0.8-12 parts of sanchi flowers, 4-6 parts of dried ginger, 4-6 parts of cinnamon, 2-4 parts of L-cysteine, 0.8-1.2 parts of amomum tsao-ko and 2-4 parts of sugar. According to the drink with the effects of promoting blood circulation and warming the body, the drink has the effects of promoting blood circulation to remove meridian obstruction, warming the spleen and stomach for dispelling cold, strengthening tendons and bones, reinforcing vital energy, tonifying qi and yin, promoting salivation and nourishing blood and the like through limitation of all the natural plant raw materials and the dosage, and the effects of dispelling cold and warming the body can be achieved under the combined action of all the raw materials, so that the cold constitution can be improved, and the health of a human body is improved. Meanwhile, the drink with the effects of promoting blood circulation and warming the body, which is prepared by the application, is suitable for being drunk in winter, and the effect of quickly warming the body can be achieved.
Owner:赵为兴

Primer probe group for PCR (Polymerase Chain Reaction) detection of sika deer mycobacterium paratuberculosis and application of primer probe group

The invention relates to the technical field of biological detection, in particular to a primer probe set for PCR detection of sika deer mycobacterium paratuberculosis and application of the primer probe set. The primers disclosed by the invention are as shown in SEQ ID NO.1-2; and the probe is as shown in SEQ ID NO. 3. The primer probe group can be used for specifically detecting whether a to-be-detected sample from the sika deer contains the mycobacterium paratuberculosis or not, the detection sensitivity can reach 5 copies / mu L, a basis is provided for rapid detection of the mycobacterium paratuberculosis of the sika deer, and a basis is provided for early diagnosis of the paratuberculosis of the sika deer.
Owner:JILIN ACAD OF ANIMAL HUSBANDRY & VETERINARY SCI

Semen collection rack for artificial insemination of sika deer

The semen collection frame comprises two side frames, the two side frames are connected through a front blocking frame, the front blocking frame is used for limiting the front neck position of the sika deer, the semen collection frame further comprises a back frame, the back frame is in an inverted U shape, the opening end of the back frame vertically extends downwards, and the opening end of the back frame is connected with the front blocking frame. The back frame is arranged on the side frame and is in sliding fit with the side frame, and a limiting piece for limiting and fixing the back frame after the back frame moves downwards is arranged on the side frame. The sika deer frame has the beneficial effects that the front neck position of the sika deer can be limited through the front blocking frame, the back position of the sika deer can be limited through the sliding back frame after the sika deer enters the space between the side frames, and the position limiting effect on the sika deer is good.
Owner:HUNAN JINFENG AGRI & ANIMAL HUSBANDRY DEV CO LTD

Multi-nutrient sika deer feed and preparation process thereof

The invention provides a multi-nutrient sika deer feed and a preparation process thereof, and belongs to the technical field of animal feeds. The multi-nutrient sika deer feed is prepared from the following components in parts by weight: 25-35 parts of pasture powder, 10-20 parts of radix astragali seu hedysari extract, 15-25 parts of protein powder, 10-15 parts of carbohydrate, 5-12 parts of fat, 4-8 parts of vitamin and mineral compound, 1-5 parts of plant source probiotics, 1-3 parts of flavoring agent and 1-3 parts of dietary fiber. The preparation process of the astragalus membranaceus extract comprises the following steps: selecting high-quality astragalus membranaceus roots, slicing, cleaning and drying, and finally obtaining the high-purity extract through the steps of microwave-assisted extraction, enzymolysis, supercritical fluid extraction, nanofiltration and high-speed counter-current chromatography. The preparation technology comprises the steps of raw material crushing, mixing, microbial fermentation, granulation and drying. By controlling technological parameters, it is ensured that nutritional ingredients of the feed are evenly distributed, palatability is good, and storage is stable. The feed disclosed by the invention can effectively meet nutritional requirements of sika deer in different growth stages, promote healthy growth of the sika deer and improve culture benefits.
Owner:江西珍源康生物科技有限公司