48 results about "Renal cortex" patented technology

The invention discloses a method for segmenting renal cortex images. The method comprises the following steps of: 1, performing initial segmentation on a kidney image by using a statistical shape model algorithm to acquire grid images of an initial outer surface S' of a kidney structure; 2, establishing sub-graphs G0 and G1 for the outer surface and the inner surface of a renal cortex respectively in a narrow band close to the grid images of the initial outer surface S' so as to obtain a weighted directed graph G=(G0, G1), designing cost functions C0 and C1 by aiming at the outer surface and the inner surface of the renal cortex respectively, generating an s-t graph Gst based on the weighted directed graph G=(G0, G1) and the cost functions C0 and C1, and extracting two layers of optimal surface images, namely an outer surface image S0 and an inner surface image S1 of the renal cortex, by using a max-flow minimal cut method; and 3, generating a data image Vcortex of a renal cortex body by using the outer surface image S0 and the inner surface image S1 of the renal cortex. By the method, the renal cortex structure can be segmented accurately, and the workload for manually segmenting the renal cortex is greatly reduced, so the method has important application values in the field of segmenting the renal cortex images and ventricular wall images with multi-surface structural characteristics.

The invention discloses an application of diacerein to prepare medicines for preventing and treating diabetic nephropathy. Diacerein is taken as a raw material of an active pharmaceutical ingredient and combined with a pharmaceutically-acceptable adjuvant to prepare a pharmaceutical composition for preventing and treating diabetic nephropathy, wherein the composition contains an effective treatment dose of the diacerein and a pharmaceutically-acceptable carrier. A research on studying the diacerein capability of protecting a diabetic rat kidney through adopting streptozotocin to induce an experimental diabetic rat model accidentally discovers that the diacerein can reduce the glycated hemoglobin level and urinary albumin excretion rate of the blood of the diabetic rat, inhibits renal cortex protein non-enzyme advanced glycation end products from forming and improves renal pathology and lesion.

The invention relates to the use of human bendazac lysine in pharmaceutical industry, wherein the bendazac lysine possesses substantial actions for reducing hypoglycemia and saccharifying hemoglobin and urine protein, thus can be applied to the prevention and treatment of diabetes.

The invention discloses a three-dimensional renal cortex positioning method based on a three-dimensional active contour model and three-dimensional Harvard transformation. The method includes the model establishing training part and the positioning part. The model establishing training part includes the steps that 10-20 sets of three-dimensional data are selected as training data; slices are selected through interpolation correction; marking points are drawn; a model is established through the three-dimensional active contour model, and the model is obtained and comprises an abdominal cavity outer contour, a renal cortex outer surface and a renal cortex inner surface; a kidney contour is stored in an R-table. The positioning part includes the step of searching positioning and the step of positioning the renal cortex; in the step of searching positioning, the kidney contour stored in the R-table is used for positioning the gravity center point of the whole kidney through the three-dimensional Harvard transformation; when the renal cortex is positioned, the model obtained through three-dimensional active contour model establishing is placed in the found gravity center of the kidney, and parameterization is performed and the range is regulated to be smaller so that searching can be performed on the gravity center point till the positioning result is obtained. With the method, the specific position of the renal cortex can be fast and effectively positioned.

The invention discloses an application of a low-dose ursolic acid as a medicament for treating diabetic early nephropathy. The administration mode of the ursolic acid is oral administration, and the administration dose is 50-70mg / kg weight. The invention verifies that the oral administration of the low-dose ursolic acid has a remarkable inhibition action for the activation of ERK1 / 2 and JNK accesses in an MAPK access in a renal cortex induced by diabetes and the phosphorylation level of STAT3 tyrosine and can markedly lower the expression of iNOS in the renal cortex induced by the diabetes, and more importantly, the oral administration of the ursolic acid can inhabit the glomerular hypertrophy caused by the diabetes and the accumulation of type IV collagen at glomerulus, thereby indicating that the oral administration of the ursolic acid has a remarkable therapeutic effect on the diabetic early nephropathy.

The invention discloses a renal cortex locating method based on a statistical shape model. The method includes a training stage and a renal cortex locating stage. The method is characterized in that in the training stage, artificial marks L1 and L2 of a kidney of each three-dimensional CT image in a training data set are made, binary data in L1 and L2 marking regions is converted to surface data M1 and M2 in a corresponding manner, interior surface data of renal cortex is calculated, and a renal cortex statistical shape model is established. According to the method, in the training stage, the renal cortex statistical shape model is established to perform statistics on the change mode of renal cortex, and the accuracy and the rapidity of locating of renal cortex are improved by employing an iteration model deformation algorithm.

The invention discloses a three-dimensional perfusion type cell culture device and an application thereof. The three-dimensional perfusion type cell culture device comprises a box body, a cover body and an elastic clamp, wherein a first circular groove is formed in the top surface of the box body, and a second circular groove with the same radius as the first circular groove is formed in the bottom surface of the cover body. In the cell culture device disclosed by the invention, vessels consisting of polyester fibers are arranged on the bottom surface of the groove, and a cell or tissue explant is positioned between two layers of vessels to form 'sandwich' culture; the vessels consisting of polyester fibers are used for replacing a scaffold material, so that the dead angle volume in cell culture is minimized to better conform to the in-vivo physiological microenvironment. A culture solution inlet channel and a culture solution outlet channel are formed on the side wall of the box body of the culture device respectively, and a culture solution flows into the groove cavity through the solution inlet channel and flows out through the solution outlet channel so as to realize perfusion culture. By adopting the device disclosed by the invention, the embryonic stem cell / progenitor cell at the new renal cortex part is successfully differentiated into renal tubule tissues.

A drug carrier nanoparticle has been synthesized that can specifically target the proximal tubules of the kidneys. The nanoparticles accumulate in the kidneys to a greater extent than other organs (e.g., up to 3 or more times greater in the kidney than any other organ). They can encapsulate many classes of drug molecules. The nanoparticles are biodegradable and release the drug as they degrade. The particles can sustainably release a drug within the kidneys for up to two months. The nanoparticles are useful for the treatment of diseases that affect the proximal tubules, such as heart failure, liver cirrhosis, hypertension, and renal failure; the study of relative blood flow to the renal cortex and medulla; and delivery of agents to treat gout.

The invention discloses a folding electrode resectoscope. The resectoscope comprises an endoscope, an operating handle and an electric cutter, the endoscope comprises an endoscope rod, and the operating handle detachably sleeves the endoscope rod; the electric cutter comprises a cutting electrode and a supporting rod connected with the cutting electrode, the cutting electrode is arranged on the end portion of the supporting rod, an electrode steering structure enabling the end portion of the supporting rod to rotate along an arc path is arranged on the supporting rod, and the electrode steering structure comprises a node structure enabling the end portion to rotate around an axis and a traction structure connected with the end portion and the operating handle; through operation on the operating handle connected with the traction structure, the supporting rod can be driven to rotate in a folding mode, the cutting electrode can conduct cutting along the radian of a sac wall, the risks ofsac wall bleeding, damage to a renal cortex, fat liquefaction and the like are reduced, the defect that an existing electrode resectoscope can only linearly conduct cutting forward and backward is remedied, operation is easy and convenient, and the operation efficiency is improved while wounds are reduced.

The invention relates to the technical field of cell engineering, and particularly discloses a construction method and application of a sika deer immortalized renal epithelial cell line, and the construction method comprises the following steps: taking renal epithelial primary cells from healthy sika deer renal cortex tissues to obtain primary renal epithelial cells; treating the primary renal epithelial cells with trypsin to obtain a cell suspension; inoculating the cell suspension into a complete culture medium, and transfecting with lentivirus loaded with SV40 large T antigen genes to obtain transfected cells; 3 [mu] g / mL puromycin is applied to the transfected cells for selective culture, so that non-transfected cells are eliminated, and drug-resistant cells are obtained; carrying out continuous passage on the drug-resistant cells for at least 30 generations to obtain the immortalized renal epithelial cell line of the sika deer; according to the invention, through a specific action mechanism (inhibiting a p53 / pRb pathway and blocking cell cycle exit) of the SV40 large T antigen, a proliferation limit caused by inhibition of telomerase activity of primary cells of the cervidae animals is overcome, and a cell resource library capable of realizing continuous passage is established.

The invention belongs to the technical field of medicine, and relates to a 15-methylene-14-deoxy-11,12-dehydro-andrographolide derivative. Experiments prove that the compounds obviously inhibit the migration and activation of hepatic stellate cells, obviously inhibit the mesenchymal transition of TGF-beta 1-induced human alveolar type II epithelial cells A549, obviously inhibit the mesenchymal transition of human renal cortex proximal convoluted tubule epithelial cells HK-2, and obviously inhibit the migration of angiotensin II (Ang II)-induced primary human cardiac fibroblasts HCFB. The compound is taken as an active ingredient for preparing anti-fibrosis drugs, has high efficiency and low toxicity, provides a new drug route for the treatment and prevention of fibrosis-related diseases, and thus the selectable range of clinical drugs is expanded, so that the derivative has a good application and development prospect.

The invention discloses a non-uniform graph search partitioning algorithm used for renal cortex positioning. The algorithm is characterized by comprising steps of (1), defining a multi-scale boundary function when segmentation starts so as to realize non-uniform sampling on nodes; (2), defining different weights according to characteristics of renal cortex during segmentation and using a max-flow / minimal-cut algorithm for detecting an optimal surface for segmentation. The segmentation speed is high and the segmentation accuracy is high according to the invention.

The invention discloses a unicapsular nephrostomy tube preventing and treating percutaneous nephroscopy postoperative bleeding; the unicapsular nephrostomy tube mainly comprises a nephrostomy tube and a renal cortex airbag; the renal plevis end of the nephrostomy tube is provided with an opening so as to discharge urine and small calculus outside human body; a plurality of nephrostomy tube side wall drainage ports are arranged between the nephrostomy tube and the renal cortex airbag, thus ensuring the urine to be smoothly drained; the renal cortex airbag is sleeved on the tube wall of the nephrostomy tube in a position having certain distance from the front end of the nephrostomy tube; the renal cortex airbag is connected with a renal cortex airbag air inlet; one end of the renal cortex airbag air inlet is provided with a spring clip.

The invention discloses an E-cadherin methylation detection method based on a hydrosulphite sequencing method. The E-cadherin methylation detection method includes the steps that firstly, rats which are 6-7 weeks old are selected, the kidneys of the killed rats are collected, connective tissue is sheared off, the renal cortex is taken and sheared into 50-100 mg tissue fragments, and the tissue fragments are quick-frozen with liquid nitrogen and stored in a -80 DEG C refrigerator; secondly, genome DNA is extracted with a genome DNA extraction reagent kit; thirdly, the concentration and purity of genome DNA are measured with a nucleic acid quantometer, the integrity of genome DNA is detected through 0.5%-1.0% sepharose gel electrophoresis, and DNA samples detected to be qualified are placed in a -20 DEG C--80 DEG C refrigerator for use; fourthly, genome DNA is modified with a methylation conversion reagent kit, and the concentration of modified genome DNA is determined with the nucleic acid quantometer; fifthly, PCR amplification is carried out under different experiment conditions; sixthly, samples used in follow-up steps are selected in the fifth step. By means of the E-cadherin methylation detection method, the success rate of E-cadherin methylation experiments is increased.

The invention provides a kidney needle biopsy quantitative teaching evaluation system and method based on mixed reality, and the system comprises a virtual-real space registration unit which is used for building the space mapping between a virtual kidney model containing a renal cortex layered anatomical structure and a simulation entity training model body, and completing the space registration of the virtual model and the entity model body; the operation data acquisition module is used for acquiring three-dimensional motion trail data of the puncture needle in the puncture operation process; according to the quantitative scoring module, the multi-dimensional weighted scoring model takes the cortical ratio as a core evaluation index, and the scoring dimension corresponding to the cortical ratio has the highest weight in the model; the quantitative scoring module calculates a cortical ratio based on the acquired three-dimensional motion trail data, and outputs an operation quantitative score through a multi-dimensional weighted scoring model; and the closed-loop verification unit is used for acquiring a real puncture result on the simulation entity training die body and comparing the real puncture result with the puncture track subjected to virtual calculation so as to calibrate the operation quantitative score.

A system and method for automatically estimating a hepatorenal index (HRI) from ultrasound images s provided. The method includes acquiring a sequence of ultrasound image data until a desired ultrasound image view is obtained. The method includes segmenting, a liver and a renal cortex in the obtained ultrasound image view. The method includes identifying valid samples in the liver and the renal cortex in the obtained ultrasound image view by excluding invalid samples. The method includes automatically positioning a liver region of interest and a renal cortex region of interest in the obtained ultrasound image view based on the valid samples and at least one criterion. The method includes determining an HRI and causing a display system to present the HRI.

The present invention is entitled "Method and system for automatically estimating the hepatorenal index from ultrasound images". The present invention provides a system and method for automatically estimating the hepatorenal index (HRI) from ultrasound images. The method includes acquiring a sequence of ultrasound image data until a desired ultrasound image view is obtained. The method includes segmenting the liver and the renal cortex in the obtained ultrasound image view. The method includes identifying valid samples in the liver and the renal cortex in the obtained ultrasound image view by excluding invalid samples. The method includes automatically locating a liver region of interest and a renal cortex region of interest in the obtained ultrasound image view based on the valid samples and at least one criterion. The method includes determining the HRI and causing a display system to present the HRI.

The invention is a simplified model on kidney rejection in animals and its setting approach. The method involves isolating dendritic cells from male mice and isolating T cells from female mice and then using the naive T cells in vitro to culture concurrently with male dendritic cells. Those activated T cells from female origin were injected into renal cortex in male mice for the purpose of attacking renal cortex. The animal model simulates the renal transplant rejection method to enable effective induction of renal transplant immune reaction.

A renal cortex targeting elastin-like polypeptide (ELP), a renal medulla and cortex targeting ELP, and a method of treating a renal disorder are provided. The renal cortex targeting ELP includes up to 95 repeat units having the sequence VPGXG (SEQ ID NO: 1), where X in each of the repeat units is any amino acid except proline. The renal medulla and cortex targeting ELP includes at least 95 repeat units of SEQ ID NO: 1, where X in each of the repeat units is any amino acid except proline. The method of treating a renal disorder includes administering an ELP and a therapeutic drug to a subject in need thereof, where the ELP includes up to 671 repeat units of SEQ ID NO: 1 and X in each of the repeat units is any amino acid except proline.

The invention discloses the use of pine needle extract in the preparation of medicines or health-care products for promoting mercury discharge. The pine needle extract is prepared by using pine needles as a raw material and by cutting, drying, grinding, extracting in nonpolar solvent, concentrating, saponifying and the like. Animal experiments indicate that the pine needle extract can obviously lower the content of mercury in renal cortex, liver and urine in rats contaminated by mercury and urea nitrogen content in the serum of the rats contaminated by mercury and has the functions of preventing mercury accumulation in body and improving functions of kidney. Therefore, the pine needle extract can be used for preparing the medicines or health-care products for promoting mercury discharge from bodies.

According to the invention, a human renal cortex proximal tubule epithelial cell HK2-CaOx model and a rat kidney stone animal model are adopted, differential modified proteins are analyzed and verified through a protein modification omics technology, and key factors and key sites of lactic acid modification participating in regulation and control of kidney stone generation are identified; single cell sequencing is combined with space transcriptome analysis, identification and subdivision of kidney cell subgroups, and differential expression ligand and receptor pairs and key signal channels of macrophages and renal tubular epithelial cells are analyzed through cell communication. On the basis, the key function of the LDHB protein lactic acid modification site K156 in the kidney stone progress process is identified, and a new molecular marker and a treatment target are provided for early diagnosis of kidney stone and intervention of kidney stone.

The invention relates to the technical field of image processing, in particular to a method for evaluating the renal function of a nephrotic patient through diffusion tensor imaging. The method comprises the following steps: acquiring a kidney diffusion tensor original image through single excitation echo plane imaging; adjacent comparison is carried out on each pixel point, and a signal intensity difference and a gradient direction difference are extracted; judging local geometric distortion according to the difference information, and carrying out correction processing pixel by pixel to obtain a corrected high-quality diffusion tensor image; and finally, calculating a fitting tensor, extracting diffusion parameters of the renal cortex and the renal medullary region, quantitatively comparing the diffusion parameters with preset renal function indexes, and outputting an objective quantitative result of the renal function of the patient. According to the method, through pixel-by-pixel distortion correction and direction feature extraction, the precision of the diffusion tensor image and the accuracy of kidney region division are improved, and objective quantitative analysis of kidney functions is realized.

The invention discloses a non-uniform graph search partitioning algorithm used for renal cortex positioning. The algorithm is characterized by comprising steps of (1), defining a multi-scale boundary function when segmentation starts so as to realize non-uniform sampling on nodes; (2), defining different weights according to characteristics of renal cortex during segmentation and using a max-flow / minimal-cut algorithm for detecting an optimal surface for segmentation. The segmentation speed is high and the segmentation accuracy is high according to the invention.

The invention provides application of an LEF1 expression inhibitor in preparation of a medicine for treating renal fibrosis. The rAAV9-mKsp-promoter-mLef1-3 shRNA adeno-associated viruses and the rAAV9-mKsp-promoter-hLEF1-3 shRNA adeno-associated viruses are constructed, the expression of LEF1 in renal tubular epithelial cells is inhibited through renal cortex in-situ injection, the targeted renal tubular epithelial cells LEF1 are used for treating unilateral ureteral obstruction renal fibrosis model mice, the renal fibrosis lesion is relieved, and the important clinical application value is achieved for the treatment of renal fibrosis diseases.

The invention belongs to the technical field of medicine and relates to 15-methylene-14-deoxy-11,12-dehydro-andrographolide derivatives. It has been proved by experiments that this type of compound significantly inhibits the migration and activation of hepatic stellate cells; significantly inhibits TGF‑β1-induced mesenchymal transition of human alveolar type II epithelial cells A549; significantly inhibits human renal cortical proximal tubule epithelial cells HK‑2 mesenchymal transition; significantly inhibits angiotensin Ⅱ (AngⅡ)-induced migration of primary human cardiac fibroblasts HCFB. Using this type of compound as an active ingredient to prepare anti-fibrosis drugs has high efficiency and low toxicity, and provides a new drug approach for the treatment and prevention of fibrosis-related diseases, thereby expanding the optional range of clinical drugs and having good application development prospect.

A drug carrier nanoparticle has been synthesized that can specifically target the proximal tubules of the kidneys. The nanoparticles accumulate in the kidneys to a greater extent than other organs (e.g., up to 3 or more times greater in the kidney than any other organ). They can encapsulate many classes of drug molecules. The nanoparticles are biodegradable and release the drug as they degrade. The particles can sustainably release a drug within the kidneys for up to two months. The nanoparticles are useful for the treatment of diseases that affect the proximal tubules, such as heart failure, liver cirrhosis, hypertension, and renal failure; the study of relative blood flow to the renal cortex and medulla; and delivery of agents to treat gout.

The invention discloses a renal cortex locating method based on a statistical shape model. The method includes a training stage and a renal cortex locating stage. The method is characterized in that in the training stage, artificial marks L1 and L2 of a kidney of each three-dimensional CT image in a training data set are made, binary data in L1 and L2 marking regions is converted to surface data M1 and M2 in a corresponding manner, interior surface data of renal cortex is calculated, and a renal cortex statistical shape model is established. According to the method, in the training stage, the renal cortex statistical shape model is established to perform statistics on the change mode of renal cortex, and the accuracy and the rapidity of locating of renal cortex are improved by employing an iteration model deformation algorithm.

Embodiments of the present application provide a kidney state evaluation device, method, equipment, storage medium and program product. In the device: a obtaining module obtains a kidney structure image, an RBF image and a T1Map; a registration module performs image registration on the kidney structure image, the RBF image and the T1Map to obtain a target kidney structure image, a target RBF image and a target T1Map; a segmentation module segments the target kidney structure image by using a trained kidney segmentation model to obtain a segmentation mask of the renal parenchyma, renal cortex and renal medulla respectively; a processing module determines the morphology of the renal parenchyma from the target kidney structure image, determines the blood perfusion values of the renal cortex and the renal medulla from the target RBF image, and determines the T1 relaxation times of the renal cortex and the renal medulla from the target T1Map based on the segmentation mask; and an evaluation module evaluates the kidney state according to the processing result. The scheme provided in the embodiments of the present application can improve the accuracy of kidney state evaluation.

The invention relates to the technical field of medical ultrasound, and discloses a method and device for measuring an ultrasonic liver and kidney index and ultrasonic equipment, and the method comprises the steps: obtaining an ultrasonic image corresponding to an ultrasonic echo signal of a liver and kidney part of a target object; segmenting the ultrasonic image to obtain a first segmentation mask of a liver parenchyma area and a second segmentation mask of a renal cortex area; post-processing the ultrasonic image, the first segmentation mask and the second segmentation mask to obtain a first target region of interest in liver parenchyma and a second target region of interest in renal cortex; and calculating the liver and kidney index according to the first target region of interest and the second target region of interest. The first segmentation mask of the liver parenchyma area and the second segmentation mask of the renal cortex area are obtained by segmenting the ultrasonic image, complete liver organ and kidney organ areas do not need to be segmented, errors possibly introduced when the whole organ is analyzed are avoided, and the measurement precision of the liver and kidney indexes is improved.

The invention belongs to the technical field of medicine, and discloses the application of 15-benzylidene-14-deoxy-11,12-dehydroandrographolide derivatives in the preparation of drugs for preventing and treating human organ or tissue fibrosis. Experiments have proved that these compounds significantly inhibit TGF-β1-induced mesenchymal transition of human alveolar type II epithelial cells A549; significantly inhibit TGF-β1-induced mesenchymal transition of human renal cortical proximal tubule epithelial cells HK-2; inhibit Angiotensin Ⅱ (Ang Ⅱ)-induced proliferation of human primary cardiac fibrosis cells HCFB. Significantly reduce the degree of bleomycin-induced pulmonary fibrosis in mice, significantly reduce the degree of renal fibrosis in rats induced by unilateral ureteral ligation and the degree of myocardial fibrosis in Kunming mice induced by isoproterenol ISO. Using this type of compound as an active ingredient to prepare anti-fibrosis drugs such as kidney, lung, heart, etc., has high efficiency and low toxicity, and provides a new drug approach for the treatment and prevention of diseases related to fibrosis, thereby expanding the choice of clinical medication range, has a good prospect for application development.