Three-dimensional perfusion type cell culture device and application thereof

A cell culture and perfusion technology, applied in the field of cell culture equipment or devices, can solve the problems of short dialysis life, high medical costs, high mortality from acute and chronic renal failure, and achieve the goal of reducing culture costs, high prices, and reducing dead space volume. Effect
CN103740591AActive Publication Date: 2014-04-23康珞生物科技(武汉)有限公司
2 Cites 3 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
康珞生物科技(武汉)有限公司
Publication Date
2014-04-23

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a three-dimensional perfusion type cell culture device and an application thereof. The three-dimensional perfusion type cell culture device comprises a box body, a cover body and an elastic clamp, wherein a first circular groove is formed in the top surface of the box body, and a second circular groove with the same radius as the first circular groove is formed in the bottom surface of the cover body. In the cell culture device disclosed by the invention, vessels consisting of polyester fibers are arranged on the bottom surface of the groove, and a cell or tissue explant is positioned between two layers of vessels to form 'sandwich' culture; the vessels consisting of polyester fibers are used for replacing a scaffold material, so that the dead angle volume in cell culture is minimized to better conform to the in-vivo physiological microenvironment. A culture solution inlet channel and a culture solution outlet channel are formed on the side wall of the box body of the culture device respectively, and a culture solution flows into the groove cavity through the solution inlet channel and flows out through the solution outlet channel so as to realize perfusion culture. By adopting the device disclosed by the invention, the embryonic stem cell / progenitor cell at the new renal cortex part is successfully differentiated into renal tubule tissues.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of cell culture equipment or devices, and in particular relates to a three-dimensional perfusion cell culture device, and also relates to the use of a three-dimensional perfusion cell culture device. / A cell culture vessel for successful differentiation of progenitor cells into renal tubular tissue. Background technique

[0002] Since Willhelm Roux isolated cells from chicken embryos in 1885 to establish in vitro cell culture for the first time, monolayer cell culture technology has a history of more than 100 years. For more than a century, monolayer cell culture has flourished. Especially in industrial fields such as pharmaceuticals or vaccine synthesis, products can be manufactured efficiently through rapid cell division. However, in the field of basic life science research, for the in vitro culture of cells, the focus is not only on their division and growth, but more importantly, whether they can maintain their...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More