The invention provides a telomerase activity detecting probe, a telomerase activity detecting reagent kit and a telomerase activity detecting method. The design of telomerase combination primers (TS) and reverse primers is simple, and TS only needs to comprise DNA (deoxyribonucleic acid) enzyme sequences and restriction enzyme cutting sites of cutting enzyme and does not need any modification; the reverse primers only need to comprise sequences complemented with multiple-G sequences extending from telomerase, so the primers are easy to design, and the feasibility is high. The telomerase activity detecting method provided by the invention has the advantages that the reverse primers are only combined with TS primers extending from result telomerase, the nonspecific amplification is reduced, in addition, in the chemical luminous reaction, only amplified telomerase multiple-G sequences and DNA enzyme can be combined with hemin to form a tetramer, and chemical luminous signals are generated, so the telomerase activity detecting method provided by the invention has higher specificity and higher sensitivity. In addition, the telomerase activity detecting reagent kit provided by the invention has the advantages that the cost is low, the operation is simple, and the time is saved.