Zooplankton rrnL gene amplification primers and screening method, application and application method thereof

A technology for zooplankton and gene amplification, applied in biochemical equipment and methods, special data processing applications, microbial measurement/inspection, etc., can solve the harsh PCR reaction conditions, poor amplification ability of crustacean groups, difficult sequencing, etc. problems, to achieve the effect of improving the success rate of PCR, mild conditions, and reducing non-specific amplification
CN104404154AActive Publication Date: 2015-03-11南京易基诺环保科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
南京易基诺环保科技有限公司
Publication Date
2015-03-11

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Abstract

The invention discloses zooplankton rrnL gene amplification primers and a screening method, application and an application method thereof, and belongs to the biotechnical field. According to common zooplankton rrnL gene sequences, 9 PCR (polymerase chain reaction) amplification primers are designed, comprising 5 upstream primers and 4 downstream primers; the primers have wide coverage and high amplification efficiency for zooplankton, and PCR products of the primers are compatible with a high-throughput sequencing platform; after the upstream primers and the downstream primers are combined in pairs, products with the lengths of 190-390 bp can be amplified; by a multi-primer combination method, nonspecific amplification can be reduced and the success rate of PCR can be improved; the PCR products can be widely used for performing species identification, biodiversity analysis and other researches through Barcode sequences.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and more specifically relates to a zooplankton rrnL gene amplification primer and a screening method, application and application method thereof. Background technique

[0002] DNA barcoding (DNA-barcoding) is currently one of the most powerful species identification techniques, especially playing an important role in the identification of unknown species. With the improvement of next-generation sequencing throughput and the reduction of sequencing costs, DNA barcoding technology has begun to be more and more applied in the investigation of environmental biodiversity. At present, species identification based on DNA barcodes relies on the amplification process of PCR, which first amplifies DNA fragments that can distinguish species, and then identifies species based on sequence differences. At present, the DNA barcode used to identify animals is mainly the mitochondrial cytochrome oxidase I (COI) gene...

Claims

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