42 results about "Dna barcodes" patented technology

The invention provides a method and a PCR (polymerase chain reaction) reagent kit for identifying DNA (deoxyribonucleic acid) barcodes of animal medicinal materials. The PCR reagent kit comprises PCR enhancers. The PCR enhancers comprise bovine serum albumin, dithiothreitol, betaine and nonidet p40. The method and the PCR reagent kit for identifying the DNA barcodes of the animal medicinal materials have the advantages that low-abundance traditional Chinese medicine samples with difficulty in amplification, particularly, samples with difficulty in identifying DNA barcodes, such as samples of buffalo horns, antelope horns, tortoise shells, carapax trionycis, pangolins and snake slough, can be effectively amplified, accordingly, the amplification success rate which is close to 100%, of DNA barcode identification techniques can be guaranteed, and DNA barcode techniques can be widely applied.

The invention provides substrates that have antibodies and aptamers bound thereto. The invention also provides methods of detecting target analytes in a sample comprising detecting binding of a target analyte to capture probes on a substrate, wherein some of the capture probes comprise antibodies and other capture probes comprise aptamers, and all of the capture probes are bound to the substrate. In addition, the invention provides substrates that have capture probes and capture oligonucleotides bound thereto, wherein the capture oligonucleotides can hybridize to DNA barcodes. The invention also provides methods of detecting target analytes in a sample comprising contacting the sample with a substrate that has capture probes and capture oligonucleotides bound thereto.

A method for detecting a target biological material using DNA barcodes is provided. The method comprises: attaching a first type of probes to the surface of magnetic particles to prepare magnetic particles for isolation wherein the first type of probes are at least partially complementary to a target biological material of interest; attaching a second type of probes to the surface of polymer particles to prepare polymer particles for analysis wherein the second type of probes are at least partially complementary to the target material but are different from the first type of probes, and attaching DNA barcodes as identification codes to the surface of the polymer particles wherein the DNA barcodes are present in an amount at least three times the amount of the second type of probes, have a predetermined sequence and are labeled with a label material; reacting the magnetic particles for isolation, the polymer particles for analysis and the target material in a hybridization reaction buffer to prepare composites, each of which consists of one magnetic particle for isolation, the target material and one polymer particle for analysis; separating the composites from unreacted reactants using a magnetic separator; heating the separated composites to denature the DNA barcodes present in the polymer particles for analysis and removing the magnetic particles for isolation and the polymer particles for analysis from the composites by centrifugation to isolate the DNA barcodes; and detecting a signal generated from the label material bound to the isolated DNA barcodes.

The invention provides a new combination of two pairs of universal amplification primers for plant parasitic nematode ribosome ITS (internal transcribed spacer) and an application method thereof for PCR (polymerase chain reaction) amplification. The key innovation point of the method is that after the new primer combination is applied to the PCR amplification of the plant parasitic nematode ITS, the plant nematode type which can be amplified by the new PCR amplification system has broader spectrum, the amplification efficiency and success rate are remarkably improved, and the problems of the ITS amplification in the plant nematode identification before are effectively solved. The PCR amplification product amplified by the method provided by the invention meets the needs of the molecular detection technologies such as nematode DNA (deoxyribonucleic acid) barcode identification and RFLP (restricted fragment length polymorphism) enzyme digestion identification, and can be popularized to the quarantine identification of the port and agriculture/forestry departments in China.

The invention discloses an application of trnH-psbA sequences as DNA barcodes in identifying Trapa acornis Nakano and an identifying method. The identifying method comprises the following steps: amplifying trnH-psbA sequences of to-be-detected samples by using PCR (polymerase chain reaction) and sequencing, if a 278bp base of the trnH-psbA sequence is A and a 308bp base is T, determining that the to-be-detected samples are Trapa acornis Nakano. Compared with the traditional morphological identification method and DNA fingerprint marking, in the invention, trnH-psbA sequences can be used as DNA barcodes for identifying Trapa acornis Nakano, so that through carrying out PCR amplification and sequencing on the trnH-psbA sequences, identification results can be directly obtained, and the identifying method is high in detection accuracy, high in repeatability and short in identification time.

The invention discloses a bacterial nucleic acid sequencing and identification system and method for intelligent DNA barcodes, and relates to the technical field of DNA sequencing and identification.Through the system and method, technical research on the DNA barcode database and detection and identification traceability thereof of food poisoning sources can be conveniently realized, so that foodsafety can be guaranteed, the meal selection of people can be guided, the occurrence of poisoning events can be prevented, and as the nucleic acid sequencing identification method of common bacteriais established, convenient DNA barcode sequencing and identification of bacteria, cells and viruses in the biological field can be achieved; operation can be performed on a DNA genome in a special wayduring extraction and purification, so that the dissociation of a DNA sequence can be avoided. Through the adoption of a constructed standard nucleic acid sequence, a standard nucleic acid sequence database for bacterial nucleic acid sequencing and identification can be input according to a unified common description and normative codes, so that objective and accurate judgment bases can be provided for the nucleic acid sequencing identification method.

Provided is a method for extracting and characterizing molecular clones. The method includes: providing a substrate on which molecular clones are formed; applying energy to desired ones of the molecular clones in a non-contact mode to extract the desired molecular clones from the substrate; chemically linking DNA barcodes to the sequences of the extracted molecular clones; and determining the DNA barcode-linked sequences by parallel sequencing.

The invention discloses a molecular identification primer, kit and method for five groupers. The invention firstly provides the molecular identification primer for five groupers. The nucleotide sequence of the primer is as shown in SEQ ID NO: 1-2; and the five groupers are any one or more of Yunlong hybrid spot, tiger dragon hybrid spot, red dragon hybrid spot, epinephelus akaara or epinephelus coioides. By utilizing the primer, the five groupers can be quickly and effectively identified by adopting a method of combining mitochondrial genome DNA barcodes and a restriction endonuclease technology, and the primers are stable in reaction and good in repeatability; compared with the traditional morphological identification, the method has the characteristics of high detection accuracy and objectivity; compared with a simple mitochondrial genome DNA bar code sequencing identification method, the method has the characteristics of strong intuition and simple judgment; and therefore, the primer provided by the invention has a wide application prospect in identifying the five groupers or preparing a kit for identifying the five groupers.

The invention discloses a leptobotia elongate DNA barcode sequence and an application thereof and belongs to the technical field of species identification. The leptobotia elongate DNA barcodes refer to leptobotia elongate COI genes, the DNA barcode sequence can serve as a standard detection sequence of leptobotia elongate DNA, and endemic species of the leptobotia elongate can be effectively identified.

Disclosed herein include systems, methods, compositions, and kits for in situ readout of barcodes, such as DNA barcodes. Barcode constructs containing a promoter (e.g., a phage promoter) that is inactive in live cells can be integrated in the genomes of cells. Cells can be fixed, and phage RNA polymerase can be used for transcription of the barcode to RNA transcripts. The RNA transcripts can be detected using, for example, fluorescent imaging and used to determine barcode sequences.

The invention discloses application of a COI gene in identification of luehdorfia chinensis, and provides a plurality of analysis methods compared with a traditional DNA bar code analysis method, andthe bar code screened by the method is higher in accuracy. The invention also discloses an analysis method for evaluating the applicability of luehdorfia chinensis COI gene as a gene bar code. By means of the analysis method, two proper COI gene bar codes are provided, rapid and accurate identification of different insect states (including adults, eggs, larvae and pupae) and incomplete specimens (including partial tissues of muscles, feet, wings and body walls) is facilitated, and the identification time is shortened. The analysis method has important reference significance for screening DNA barcodes, and has a great application prospect.

The invention discloses a method for tracing pork through gene fluorescence labeling. The method comprises the following steps: step 1, extracting genome DNA (Deoxyribonucleic Acid) in tissues of pig individuals; step 2, carrying out fluorescence labeling on four types of dideoxynucleotide of the genome DNA respectively; step 3, carrying out electrophoresis on the genome DNA to mutually separate DNA molecules with different lengths; labeling the DNA molecules respectively; step 4, irradiating with ultraviolet rays, wherein the DNA molecules of the four types of labeled dideoxynucleotide emit fluorescent light of different wavelengths; DNA molecule sequences can be distinguished by analyzing light spectrums of the fluorescent light; step 5, grouping and compiling the DNA molecule sequences to form DNA barcodes corresponding to the pig individuals by combining gene types corresponding to the DNA molecules, which are generated through the electrophoresis in step 3, of the four types of dideoxynucleotide. According to the method disclosed by the invention, pork tracing detection is carried out in links including production, slaughtering, processing, sales and the like of pork; manual intervention factors are effectively avoided and the safety is ensured when people eat the pork.