The invention belongs to the technical field of
plant gene molecular markers, and particularly relates to
a DNA bar code for identifying
nitraria tangutorum and
nitraria sibirica and application of the
DNA bar code. The
DNA bar code for identifying the
nitraria tangutorum and the nitraria sibirica comprises a specific variation site combination located in an internal transcriptional spacer (ITS),
a site matrix with species diagnosability is formed, the sites have stable species specificity, the
nitraria tangutorum and the nitraria sibirica can be rapidly identified by detecting a
nucleotide sequence of an ITS2 region of a sample, and the
DNA bar code for identifying the
nitraria tangutorum and the nitraria sibirica can be used for identifying the
nitraria tangutorum and the nitraria sibirica. Particularly, the identification accuracy is remarkably improved under the condition that morphological identification is not feasible or uncertain, so that collection, conservation and related scientific research work of
germplasm resources are facilitated, and a convenient method is provided for
germplasm identification and
resource protection.