4 results about "RpoB" patented technology

The application provides a primer probe set and a kit for detecting a rifampicin-resistant gene of Mycobacterium tuberculosis, and belongs to the technical field of molecular biology detection.The primer probe set comprises RAA primers and qPCR primers targeting the rpoB gene, probe primers targeting the wild-type rpoB gene, and probe primers targeting the mutant rpoB gene.The application adopts the detection methods of RT-RAA and qPCR in sequence, and the above specific primer probe set can be combined to simultaneously and rapidly detect the 516, 526, 531 and 533 sites of the rpoB gene of Mycobacterium tuberculosis, has good specificity, and the detection sensitivity can reach 5 copies / ul, is 20 times higher than the sensitivity of qPCR (100 copies / ul), and has a 5% detection capability for heterogenic drug resistance mutation; the detection method can also greatly shorten the detection time.

A strain of bacteria, HITLi7, based on the quantification of conserved gene abundance. T A rapid and specific detection method is involved, which uses the determination of the abundance of species-specific conserved genes to quickly detect HITLi7. T This invention addresses the challenges of complex microbial composition and the limited availability of HITLi7 strains in current bio-enhanced activated carbon processes. T The technical challenge of rapidly and specifically identifying and detecting abundance changes in indigenous bacteria presents a problem. This invention utilizes the double-positive reaction of the bispecific conserved genes gyrB and rpoB to further enhance the specificity of the detection results. The high sensitivity and accuracy based on qPCR technology further strengthen the reliability of the results. The detection method of this invention provides highly targeted results, avoiding the large amount of redundant data and complex processing procedures associated with high-throughput technologies. Compared to detection methods based on traditional bacterial morphology and physicochemical properties, it has wider applicability, higher sensitivity, and greater accuracy.

The application belongs to the technical field of genetic engineering and enzyme engineering, and particularly relates to a genetically engineered bacterium for stably and efficiently producing L-isoleucine as well as a construction method and application thereof. rpoB M The genetically engineered bacterium takes E. coli THRD as a host and contains a gene encoding an RNA polymerase beta-subunit mutant The amino acid sequence of the RNA polymerase beta-subunit mutant is sequentially replaced by leucine at the 618th amino acid residue from the N terminal to the C terminal direction compared with the parent RNA polymerase beta-subunit. The genetically engineered bacterium has good industrial application prospects and can tolerate L-isoleucine and accumulate less alpha-ketobutyric acid.

The invention belongs to the technical field of immunological detection, and particularly relates to a primer probe composition for detecting drug resistance of mycobacterium tuberculosis complex rifampicin and isoniazide and application of the primer probe composition. A multicolor melting curve method is adopted, rifampicin drug-resistant gene mutation detection is carried out by detecting mutation of drug-resistant determining regions of 507-533 sites of a mycobacterium tuberculosis complex rpoB gene, and isoniazide drug-resistant gene mutation detection is carried out by detecting mutation of ahpC promoter regions, inhA promoter regions and katG315 codons. The drug resistance condition of the detected strain can be judged according to the Tm value of the melting curve. The result of the embodiment shows that the rifampicin drug resistance detection and the isoniazide drug resistance detection realize the detection of mutation of one drug in one tube without separating two tubes, the drug resistance condition of one drug can be independently detected or the drug resistance conditions of two drugs can be detected by the two tubes together according to different conditions during use, the application is flexible, and the cost is low. The application market is wider.