2results about How to "Guaranteed amplification efficiency" patented technology

The invention discloses a universal molecular beacon kit for various animal-derived components, and belongs to the technical field of molecular biological detection. Aiming at gene segments with specific structural characteristics in 11 common livestock and poultry animal genomes, the invention designs a composition of a universal primer and a molecular beacon fluorescent probe. The combination can be used for carrying out real-time fluorescent PCR detection and melting curve analysis by using the kit and the method by taking genome DNA of a sample to be detected as a template. By comparing the shape of a melting peak of a detected sample and the characteristic melting temperature (Tm value) of the detected sample, 11 animal-derived components of livestock and poultry can be quickly and accurately identified under the condition of single-tube detection. Technical innovation is achieved in the field of molecular biological detection, an efficient and reliable solution is provided for animal-derived component detection, and the method has important practical value and popularization potential.

The utility model relates to a reading auxiliary circuit, a memory and electronic equipment, the reading auxiliary circuit comprises a voltage gate, the input end of the voltage gate is connected with a first driving signal, and the output end of the voltage gate is used for providing different driving voltages for a bit line and a reverse bit line connected with a sensitive amplifier; a first switching unit; the second switch unit is connected with the first switch unit; the first input end of the driver is connected with the output end of the voltage gate through a first switch unit, and the second input end of the driver is connected with the output end of the voltage gate through a second switch unit; wherein the first switch unit is used for transmitting a driving voltage on a bit line to a first input end of the driver when receiving a second driving signal; and the second switch unit is used for transmitting the driving voltage on the reverse bit line to the second input end of the driver when receiving a third driving signal. And the BL-BLB voltage difference delta V is improved through a scheme of pulling the high-bit line voltage, so that the accuracy and the stability during reading are ensured.