The utility model relates to the technical field of cleaning devices, in particular to a gold bonding wire cleaning device which comprises a cleaning box, a bottom plate, a motor, a supporting block, a winding wheel, a gold wire, a guide wheel rod and a supporting column, the bottom plate is fixedly connected with the lower surface of the cleaning box, the motor is fixedly connected with the inner wall of the cleaning box, and the supporting block is fixedly connected with the inner wall of the cleaning box. The winding wheel is arranged on the surface of the supporting block, the gold wire is connected with the surface of the winding wheel in a winding mode, the guide wheel rod is arranged on the inner wall of the cleaning box, the supporting column is fixedly connected with the inner wall of the cleaning box, and a positioning device is arranged on the surface of the supporting block. According to the winding wheel positioning device, the winding wheel is effectively positioned through the positioning device, the effect of conveniently assembling and disassembling the winding wheel is achieved, when common equipment is used, due to the fact that the positioning mode of the winding wheel is complex, operation is inconvenient when the winding wheel is replaced, and a large amount of time and manpower are consumed, the winding wheel replacing efficiency is greatly improved through the positioning device, and the labor intensity of workers is lowered. And the convenience of the equipment is improved.