80 results about "Blakeslea trispora" patented technology

The invention discloses a method for preparing beta-carotene through fermentation of Blakeslea trispora. The method comprises the following steps of: after activating Blakeslea trispora ATCC14271 (+) and ATCC14272 (-), carrying out seed culture on the activated Blakeslea trispora; inoculating the Blakeslea trispora to a fermentation medium according to a certain inoculum concentration and inoculation ratio under an optimized fermentation process condition so as to ferment the Blakeslea trispora; and finally obtaining a mycelium with higher beta-carotene content. Through the adoption of the method disclosed by the invention, a natural high-quality beta-carotene product with low cost and high titer can be obtained.

The invention discloses a fermentation method for preparing beta-carotin by using Blakeslea trispora, which comprises the following steps: (1) strain activation; (2) primary seed culture; (3) secondary seed culture; and (4) fermentation culture. The method has the advantages of low facility request, low cost input, wide raw material sources and low cost, obviously enhances the beta-carotin yield as compared with the unimproved technique, and thus, can implement large-scale production.

The invention provides a method for producing lycopene through fermentation. According to the invention, blakeslea trispora (+) and blakeslea trispora (-) strains are adopted, and slant culture is carried out. Culturing is carried out for 6-7 days under a temperature of 26-28 EG C in an incubator; after 36-46h of seed culturing, fermentation culture is carried out; betaine is added for a first time, and fermentation culture is carried out for 100-120h under a temperature of 26-28 DEG C and under a rotation speed of 200-250r/min; a blocking agent is added when fermentation is carried out for 42h, and betaine is added for a second time; and fermentation is continued until finished. A fermentation broth is filtered, and wet thalli are collected. Through vacuum lyophilization, dried thalli comprising lycopene are obtained. According to the invention, with the added betaine, thalli respiratory chain system can be effectively promoted, and thalli oxygen consumption rate can be improved. Also, osmotic pressure respiratory depression upon the thalli during the fermentation process can be effectively relieved, oxygen delivery rate can be accelerated, and energy consumption can be reduced. thalli growth rate and lycopene content are improved, and production cost is reduced. According to the method, the operation is simple. Betaine has no toxic or side effect, and is convenient and safe to use. The method is suitable for industrialized productions, and has high application values.

The invention discloses a method for improving beta-carotene produced from blakeslea trispora by virtue of organic acid, and relates to beta-carotene. The method comprises the following steps: 1) taking ATCC 14271 (+) and 14272 (-) bacterium liquids from blakeslea trispora, smearing the bacterium liquids on a corn meal agar medium and cultivating; picking two rings of positive and negative bacteria from an ATCC 14271 (+) and 14272 (-) strain slab, and respectively inoculating and cultivating in a conical flask containing a seed medium so as to obtain an ATCC 14271 (+) seed liquid and an ATCC14272 (-) seed liquid; mixing the two seed liquids and inoculating the seed liquids to a conical flask containing a fermenting medium; adding organic acid and cultivating; and after fermenting, collecting bacteria, filtering and cleaning, drying wet bacteria to constant weight so as to obtain the dry weight of cells, and detecting the content of the beta-carotene. The method disclosed by the invention is simple and easy to implement, low in cost and high in beta-carotene content.

The invention belongs to the technology field of biological feed preparation technique, and provides a novel method for producing and storing poultry biological feed. The invention comprises the cultivation of first-grade seeds and second-grade seeds with blakeslea trispora, the limited and mixed fermentation and production of positive-negative bacterial strains, the vacuum dry preparation of thalli at a relatively low temperature, the spraying dry preparation of thalli, and the storage technique and parameters of products. The production and storage method, and the preparation technique is novel, and final products are economical. Through the invention, the products rich in beta-renieratene through the dry preparation at the low temperature and the spraying preparation are directly applied to the production of poultry, thereby reducing high cost caused by processing and purification, increasing yield rate, and reducing environmental pollution caused by mycelium treatment. The feed produced by the production method is rich in beta-renieratene, protein, vitamin, etc., and is helpful to increasing the laying rate and the hatchable egg rate, raising the growth rate of the broiler chicken, reducing the death rate of the broiler chicken, and deepening the color of egg yolk.

The invention discloses a method for preparing beta-carotene from Blakeslea trispora mycelium. The method comprises extraction and recrystallization process stages. A two-step recrystallization method is adopted in the recrystallization processing stage and comprises the steps that B1, total extracting liquid is concentrated till the concentration of beta-carotene is 5500-6500 mg/L, heat preservation and standing are carried out at the temperature of -5-10 DEG C, and then filtration is carried out; B2, the filtrate obtained in the step B1 is concentrated till the concentration of beta-carotene is 5500-6500 mg/L, heat preservation and standing are carried out at the temperature of 0-15 DEG C, and then filtration is carried out. Mycelium drying is not needed, wet mycelium is directly used for extraction, the process is simple, energy consumption is saved, only single organic solvent is used in the whole process, the production process is easy to control, the cost is low, the high purity and high yield of the product can be ensured at the same time, and the method is suitable for industrial production.

The invention provides beta-carotene-containing bacterial powder or microbial oil or oil suspension. Beta-carotene is obtained by fermenting a blakeslea trispora mutant strain. The beta-carotene-containing bacterial powder or microbial oil or oil suspension is characterized in that the blakeslea trispora mutant strain is preserved in the China Center For Type Culture Collection (CCTCC) on August 8, 2014, the preservation address is Wuhan University, Wuhan city, China, and the preservation numbers are blakeslea trispora BT7251(+) CCTCC M 2014378 and blakeslea trispora BT7603(-) CCTCC M 2014379. The beta-carotene with the high yield can be obtained by conventionally fermenting and culturing the blakeslea trispora mutant strain. Meanwhile, the whole fermentation technology is simple, convenient to control and beneficial for industrialized production.

The invention relates to a method for improving the yield of lycopene by enhancing the growth of Blakeslea trispora, belonging to the field of improving liquid-submerged fermentation of aerobic microorganisms to produce secondary metabolite, in particular to a method for adding active carbon to enhance Blakeslea trispora to produce lycopene. The method comprises the following steps of: adding active carbon in a fermentation liquor, and utilizing the characteristic that active carbon absorbs gas to increase the concentration of dissolved oxygen in the fermentation liquor, improve the viscosity of the fermentation liquor, enhance the growth of Blakeslea trispora and improve the yield of lycopene by more than 150 percent than the original yield. The method has low cost, convenient operation and obvious effect, can replace liquid alkane and hydrogen peroxide as an oxygen-carrying agent, avoids toxicity of chemical substances to the strains, simplifies post purification process of the lycopene and provides good basis for industrial production.

The method provides a method for tank batch fermentation based on biological characteristics of blakeslea trispora. The method comprises eggplant bottle slant culture of mother seeds, 15L-150L-1,500L seed tank amplification culture of seed liquid, and 10m<3> fermentation tank fermentation culture in a mixed ratio of positive bacteria to negative bacteria of 1:10. Preferably, the tank batch fermentation step of the lycopene also includes an inoculation mode of eluting sporangium with aseptic normal saline and sending the sporangium to a first-class seed culture medium, and after fermentation, the lycopene yield is 800mg/L. The invention also provides a late extraction method based on physical-chemical characteristics of the lycopene, and the method comprises the steps of: pretreatment of fermentation products, extraction of the lycopene, concentration of the lycopene, crystallization of the lycopene, precipitation of the lycopene and the like. Preferably, the late extraction step of the lycopene also includes a step of triple circulating extractions and three-step precipitation, and after triple circulating extractions, the extraction ratio of the lycopene is over 85 percent, and after the three-step precipitation, the residual amount of solvents can be controlled in 50ppm.

The invention provides a method for processing a sample of natural beta-carotene or lycopene fermentation liquor produced by fermenting blakeslea trispora, which comprises the following steps of: absorbing fermentation liquor quantitatively, filling the fermentation liquor into a poly-plastic test tube, and freezing the fermentation liquor at below -10 DEG C; unfreezing the fermentation liquor naturally, adding water quantitatively, performing ultrasonic processing and centrifuging, and removing supernate; adding absolute ethanol into the centrifuged fermentation liquor for centrifuging, and removing supernate; and adding ethyl acetate for immersing at the temperature of between 45 and 80 DEG C, filtering in constant volume, and transferring the sample for detecting. The method has the advantages of short process flow, easy control of the process, complete sample immersing, high extraction efficiency and small toxicity of used solvent, and is suitable for processing the sample of the natural beta-carotene or lycopene fermentation liquor produced by fermenting the blakeslea trispora.

The invention discloses recombinant bacteria used for producing beta-carotene and a construction method utilizing a Crispr-Cas9 technology. A Ku70 gene is subjected to knockout from yarrowia lipolytica, and then phytoene synthase/lycopene cyclase (carRA), phytoene desaturase (carB), geranyl pyrophosphate synthetase (GGS1) and a 3-hydroxy-3-methyl glutaryl coenzyme A reductase (tHMG) gene take snfas a target point and are integrated in a yarrowia lipolytica gene set after knockout of the ku70 gene, wherein the phytoene synthase/lycopene cyclase (carRA) and phytoene desaturase (carB) are from blakeslea trispora, and the geranyl pyrophosphate synthetase (GGS1) and the 3-hydroxy-3-methyl glutaryl coenzyme A reductase (tHMG) gene are from the yarrowia lipolytica; the Crispr-Cas9 technology isutilized for regulating the copy number of the carRA, the carRA, the GGS1 and the tHMG, and the recombinant bacteria capable of producing the high-yield beta-carotene is constructed. After the recombinant bacteria is fermented, cultured, extracted and separated, the content of the beta-carotene can reach the dry cell weight of 35 mg/g, and the bacterial strain stability is high.

The invention provides a method or highly yielding lycopene by using Blakeslea trispora fermentation. The method comprises the steps of primary seed culture, secondary seed culture, fermenting culture, and extraction and separation. The method is characterized in that rapeseed oil is added to a fermenting culture medium to make the mass content of the rapeseed oil in the fermenting culture medium reach 2.0-5.0%, fermenting culture is carried out for 20-26h, and then nicotine accounting for 0.05-0.07% of the mass of a fermentation substance is added. Compared with the prior art, the method provided by the invention has the advantages of 10% or above increase of the lycopene yield, short production period, low cost of the fermenting culture medium, and production cost reduction.

The invention relates to Blakeslea trispora (B. trispora) strains producing at least 0.3 g/l lycopene, when co-cultivated for at least three days with a suitable strain of Blakeslea trispora of the opposite mating type in a suitable medium in the absence of an exogenous cartenogenesis inhibitor, to a natural efficient production process of lycopene by these strains, to a growth medium highly suited for this process and to an isolation process suited for any carotenoid compound.

The invention discloses blakeslea trispora, which is preserved in China General Microbiological Culture Collection Center. A method for preparing lycopene from blakeslea trispora comprises the following steps of performing activation: inoculating activation strains onto a seed culture medium to be cultured for 42 to 44 under the conditions of the temperature being 30 to 35 DEG C and the rotating speed being 150 to 220rpm for obtaining a seed culture solution; inoculating the seed culture solution onto a fermentation culture medium to be fermented for 120 to 144h at 32 to 35 DEG C under the light shielding condition; then, performing fermentation for 46 to 48h; adding a blocking agent; extracting the lycopene in the fermentation solution, wherein an extraction agent is acetone or petroleum ether, the mass ratio of the fermentation solution to the extraction agent is 1: (200 to 250), the extraction temperature is 40 to 60 DEG C, and the extraction time is 2h; extracting the extraction solution at the extraction temperature being 40 to 45 DEG C; performing overnight crystallization at -4 DEG C. Through being measured by liquid phase chromatography, the purity of the lycopene is 85 percent or higher than 85 percent. The method has the advantages that the yield of the lycopene is improved; the purity of the separated lycopene is high.

The invention belongs to the field of production of lycopene and discloses a fermentation medium, the application thereof and a method for preparing lycopene through fermentation of Blakeslea trispora. The fermentation medium comprises the following ingredients: a starch component, a bean flour component, fish meal, vegetable oil, copper sulfate, magnesium sulfate, monopotassium phosphate, vitamin E and an emulsifying agent, wherein the starch component comprises starch and/or starch hydrolysate; the bean flour component comprises bean flour and/or bean flour hydrolysate; and at least one of the starch component and the bean flour component is the hydrolysate of the starch or bean flour. With the adoption of the fermentation medium provided by the invention, the concentration of dissolved oxygen can be improved, and the dissolved oxygen can be reasonably and efficiently utilized. Therefore, the yield of lycopene is improved.

The invention relates to a method for producing lycopene through fermentation of blakeslea trispora and lycopene, and belongs to the field of fermentation engineering. The method comprises the following steps: respectively culturing positive fungi and negative fungi of the blakeslea trispora, and taking a part of cultured positive fungi and negative fungi of the blakeslea trispora and simultaneously carrying out inoculation; performing fermentation, controlling a pH value of a fermentation system to reach 5.8 to 6.2 by using a regulating agent in a fermentation process, supplementing materialsin a period of 40 to 100 hours after beginning of fermentation, continuously fermenting to 120 hours, and then releasing from a tank, wherein the regulating agent is an acidic substance which contains PO4<3+> and does not contain metallic ions or salt; a fermentation culture medium contains starch phosphate ester substances. The method has the advantages of simpleness and low cost; a fermentationpH value is controlled by the acidic substance which contains PO4<3+> and does not contain metallic ions or salt and PO4<3+> is provided, so that introduction of excessive amount of K<+> is avoided;through cooperative application of the starch phosphate ester substances, the output of the lycopene is increased; therefore, the output of the lycopene is higher, and the method is suitable for industrial production.

The invention discloses a method of preparing lycopene and a lycopene product. The method of preparing lycopene, disclosed by the invention, comprises the step of adding a bio-enzyme and a blocking agent into fermenting liquor containing positive blakeslea trispora and positive blakeslea trispora in a fermenting culture process for enzymolysis, wherein the bio-enzyme is selected from one or combination of more of pectinase, cellulase, neutral protease and lipase. By means of action of the bio-enzyme, enzymolysis is performed on adhered substances such as pectic substance, proteins and lipid among hyphae, so that the aims of reducing the viscosity of the fermenting liquor, improving the distributing degree of hyphae, and increasing dissolved oxygen in the fermenting liquor effectively so as to improve the output of the lycopene and improve the product quality are achieved.

The invention relates to the field of bioengineering, and particularly relates to a method for preparing beta-carotin by solid fermentation by using Blakeslea trispora. The method is characterized by comprising the following steps: carrying out culture fermentation by using a solid fermentation culture medium instead of a liquid fermentation culture medium in the traditional technique, directly drying the mycelia-containing solid fermentation product under reduced pressure, and extracting the beta-carotin. Compared with the liquid fermentation, the method reduces the step of filtration or centrifugation for obtaining mycelia, greatly reduces the water consumption and wastewater discharge in the technique, is beneficial to protecting the limited fresh water resource in China, and is especially suitable for popularization and application in water-deficient areas in Northern China.

The invention relates to a method for producing beta-carotene through fermentation of blakeslea trispora, and the beta-carotene, and belongs to the field of fermentation engineering. The method comprises the steps of respectively culturing blakeslea trispora positive bacteria and blakeslea trispora negative bacteria, simultaneously inoculating, fermenting, controlling a pH value of a fermentationsystem to be 5.8 to 6.2 through a modifier during a fermenting process, semi-continuously culturing, emptying when the fermenting starts for 60h, and supplementing materials. The modifier is an acidicmaterial which contains PO4<3+> and does not contain metal ions and salt. A fermentation medium adopted in the fermenting process contains a starch phosphate material. The method is simple and low-cost; through using the acidic material which contains the PO4<3+> and does not contain the metal ions and the salt for controlling a fermenting pH value and providing the PO4<3+>, excessive K<+> is prevented from being brought in; by the cooperative use of the starch phosphate material, the yield of the beta-carotene is improved. Therefore, the obtain beta-carotene is higher in yield, and the method is suitable for industrial production.