81 results about "Blakeslea trispora" patented technology

The invention relates to a method for preparing beta-carotin from Blakeslea trispora fermentation broth, which comprises the following steps of: filtering Blakeslea trispora fermentation broth to obtain wet mycelia; vacuum-drying the wet mycelia to obtain dry mycelia of which the water content is less than 10%; milling and pulverizing the dry mycelia; screening, leaching the dry mycelia with dichloromethane which is 15-20 times of the dry mycelia by mass, evaporating to concentrate the leaching liquor until the concentration of beta-carotin is not lower than 50000 mu g / ml, keeping the temperature, and filtering to obtain the wet crude product of beta-carotin crystals; and dissolving the wet crude product with dichloromethane, filtering, evaporating to concentrate until the concentration of the beta-carotin is not lower than 50000 mu g / ml, keeping the temperature, filtering, and vacuum-drying to obtain the beta-carotin crystals. The method has the advantages of short technical process,less operation steps, easy control on production process, high product content, low cost, high yield, only one solvent used in the overall process and suitability for industrial production.

The invention discloses a method for preparing beta-carotene through fermentation of Blakeslea trispora. The method comprises the following steps of: after activating Blakeslea trispora ATCC14271 (+) and ATCC14272 (-), carrying out seed culture on the activated Blakeslea trispora; inoculating the Blakeslea trispora to a fermentation medium according to a certain inoculum concentration and inoculation ratio under an optimized fermentation process condition so as to ferment the Blakeslea trispora; and finally obtaining a mycelium with higher beta-carotene content. Through the adoption of the method disclosed by the invention, a natural high-quality beta-carotene product with low cost and high titer can be obtained.

The invention discloses a fermentation method for preparing beta-carotin by using Blakeslea trispora, which comprises the following steps: (1) strain activation; (2) primary seed culture; (3) secondary seed culture; and (4) fermentation culture. The method has the advantages of low facility request, low cost input, wide raw material sources and low cost, obviously enhances the beta-carotin yield as compared with the unimproved technique, and thus, can implement large-scale production.

The invention provides a method for producing lycopene through fermentation. According to the invention, blakeslea trispora (+) and blakeslea trispora (-) strains are adopted, and slant culture is carried out. Culturing is carried out for 6-7 days under a temperature of 26-28 EG C in an incubator; after 36-46h of seed culturing, fermentation culture is carried out; betaine is added for a first time, and fermentation culture is carried out for 100-120h under a temperature of 26-28 DEG C and under a rotation speed of 200-250r / min; a blocking agent is added when fermentation is carried out for 42h, and betaine is added for a second time; and fermentation is continued until finished. A fermentation broth is filtered, and wet thalli are collected. Through vacuum lyophilization, dried thalli comprising lycopene are obtained. According to the invention, with the added betaine, thalli respiratory chain system can be effectively promoted, and thalli oxygen consumption rate can be improved. Also, osmotic pressure respiratory depression upon the thalli during the fermentation process can be effectively relieved, oxygen delivery rate can be accelerated, and energy consumption can be reduced. thalli growth rate and lycopene content are improved, and production cost is reduced. According to the method, the operation is simple. Betaine has no toxic or side effect, and is convenient and safe to use. The method is suitable for industrialized productions, and has high application values.

The invention discloses a method for improving beta-carotene produced from blakeslea trispora by virtue of organic acid, and relates to beta-carotene. The method comprises the following steps: 1) taking ATCC 14271 (+) and 14272 (-) bacterium liquids from blakeslea trispora, smearing the bacterium liquids on a corn meal agar medium and cultivating; picking two rings of positive and negative bacteria from an ATCC 14271 (+) and 14272 (-) strain slab, and respectively inoculating and cultivating in a conical flask containing a seed medium so as to obtain an ATCC 14271 (+) seed liquid and an ATCC14272 (-) seed liquid; mixing the two seed liquids and inoculating the seed liquids to a conical flask containing a fermenting medium; adding organic acid and cultivating; and after fermenting, collecting bacteria, filtering and cleaning, drying wet bacteria to constant weight so as to obtain the dry weight of cells, and detecting the content of the beta-carotene. The method disclosed by the invention is simple and easy to implement, low in cost and high in beta-carotene content.

The invention belongs to the technology field of biological feed preparation technique, and provides a novel method for producing and storing poultry biological feed. The invention comprises the cultivation of first-grade seeds and second-grade seeds with blakeslea trispora, the limited and mixed fermentation and production of positive-negative bacterial strains, the vacuum dry preparation of thalli at a relatively low temperature, the spraying dry preparation of thalli, and the storage technique and parameters of products. The production and storage method, and the preparation technique is novel, and final products are economical. Through the invention, the products rich in beta-renieratene through the dry preparation at the low temperature and the spraying preparation are directly applied to the production of poultry, thereby reducing high cost caused by processing and purification, increasing yield rate, and reducing environmental pollution caused by mycelium treatment. The feed produced by the production method is rich in beta-renieratene, protein, vitamin, etc., and is helpful to increasing the laying rate and the hatchable egg rate, raising the growth rate of the broiler chicken, reducing the death rate of the broiler chicken, and deepening the color of egg yolk.

The invention discloses a method for preparing beta-carotene from Blakeslea trispora mycelium. The method comprises extraction and recrystallization process stages. A two-step recrystallization method is adopted in the recrystallization processing stage and comprises the steps that B1, total extracting liquid is concentrated till the concentration of beta-carotene is 5500-6500 mg / L, heat preservation and standing are carried out at the temperature of -5-10 DEG C, and then filtration is carried out; B2, the filtrate obtained in the step B1 is concentrated till the concentration of beta-carotene is 5500-6500 mg / L, heat preservation and standing are carried out at the temperature of 0-15 DEG C, and then filtration is carried out. Mycelium drying is not needed, wet mycelium is directly used for extraction, the process is simple, energy consumption is saved, only single organic solvent is used in the whole process, the production process is easy to control, the cost is low, the high purity and high yield of the product can be ensured at the same time, and the method is suitable for industrial production.

The invention relates to a method for promoting lycopene biosynthesis in blakeslea trispora. The method comprises the following steps of: (1) respectively culturing blakeslea trispora (Blakeslea trispora) (+) (-) strains, and obtaining spore suspension; (2) respectively inoculating the blakeslea trispora (Blakeslea trispora) (+) (-) strains to conical flasks filled with a seed culture medium; (3) uniformly mixing the cultured positive bacteria seed solution in one bottle and negative bacteria seed solution in four bottles, and inoculating the seed solutions into a fermentation medium for fermenting; and (4) adding NAD<+> precursor substances within 24-48 hours after the beginning of the fermentation, and continuously culturing for 84 hours. The method is easy to operate, the yield of the lycopene is greatly improved, the production cost is reduced, and the method can be used for industrial production of lycopene.

The invention provides beta-carotene-containing bacterial powder or microbial oil or oil suspension. Beta-carotene is obtained by fermenting a blakeslea trispora mutant strain. The beta-carotene-containing bacterial powder or microbial oil or oil suspension is characterized in that the blakeslea trispora mutant strain is preserved in the China Center For Type Culture Collection (CCTCC) on August 8, 2014, the preservation address is Wuhan University, Wuhan city, China, and the preservation numbers are blakeslea trispora BT7251(+) CCTCC M 2014378 and blakeslea trispora BT7603(-) CCTCC M 2014379. The beta-carotene with the high yield can be obtained by conventionally fermenting and culturing the blakeslea trispora mutant strain. Meanwhile, the whole fermentation technology is simple, convenient to control and beneficial for industrialized production.

The invention relates to a method for improving the yield of lycopene by enhancing the growth of Blakeslea trispora, belonging to the field of improving liquid-submerged fermentation of aerobic microorganisms to produce secondary metabolite, in particular to a method for adding active carbon to enhance Blakeslea trispora to produce lycopene. The method comprises the following steps of: adding active carbon in a fermentation liquor, and utilizing the characteristic that active carbon absorbs gas to increase the concentration of dissolved oxygen in the fermentation liquor, improve the viscosity of the fermentation liquor, enhance the growth of Blakeslea trispora and improve the yield of lycopene by more than 150 percent than the original yield. The method has low cost, convenient operation and obvious effect, can replace liquid alkane and hydrogen peroxide as an oxygen-carrying agent, avoids toxicity of chemical substances to the strains, simplifies post purification process of the lycopene and provides good basis for industrial production.

The method provides a method for tank batch fermentation based on biological characteristics of blakeslea trispora. The method comprises eggplant bottle slant culture of mother seeds, 15L-150L-1,500L seed tank amplification culture of seed liquid, and 10m<3> fermentation tank fermentation culture in a mixed ratio of positive bacteria to negative bacteria of 1:10. Preferably, the tank batch fermentation step of the lycopene also includes an inoculation mode of eluting sporangium with aseptic normal saline and sending the sporangium to a first-class seed culture medium, and after fermentation, the lycopene yield is 800mg / L. The invention also provides a late extraction method based on physical-chemical characteristics of the lycopene, and the method comprises the steps of: pretreatment of fermentation products, extraction of the lycopene, concentration of the lycopene, crystallization of the lycopene, precipitation of the lycopene and the like. Preferably, the late extraction step of the lycopene also includes a step of triple circulating extractions and three-step precipitation, and after triple circulating extractions, the extraction ratio of the lycopene is over 85 percent, and after the three-step precipitation, the residual amount of solvents can be controlled in 50ppm.

The invention provides a method for processing a sample of natural beta-carotene or lycopene fermentation liquor produced by fermenting blakeslea trispora, which comprises the following steps of: absorbing fermentation liquor quantitatively, filling the fermentation liquor into a poly-plastic test tube, and freezing the fermentation liquor at below -10 DEG C; unfreezing the fermentation liquor naturally, adding water quantitatively, performing ultrasonic processing and centrifuging, and removing supernate; adding absolute ethanol into the centrifuged fermentation liquor for centrifuging, and removing supernate; and adding ethyl acetate for immersing at the temperature of between 45 and 80 DEG C, filtering in constant volume, and transferring the sample for detecting. The method has the advantages of short process flow, easy control of the process, complete sample immersing, high extraction efficiency and small toxicity of used solvent, and is suitable for processing the sample of the natural beta-carotene or lycopene fermentation liquor produced by fermenting the blakeslea trispora.

The invention discloses recombinant bacteria used for producing beta-carotene and a construction method utilizing a Crispr-Cas9 technology. A Ku70 gene is subjected to knockout from yarrowia lipolytica, and then phytoene synthase / lycopene cyclase (carRA), phytoene desaturase (carB), geranyl pyrophosphate synthetase (GGS1) and a 3-hydroxy-3-methyl glutaryl coenzyme A reductase (tHMG) gene take snfas a target point and are integrated in a yarrowia lipolytica gene set after knockout of the ku70 gene, wherein the phytoene synthase / lycopene cyclase (carRA) and phytoene desaturase (carB) are from blakeslea trispora, and the geranyl pyrophosphate synthetase (GGS1) and the 3-hydroxy-3-methyl glutaryl coenzyme A reductase (tHMG) gene are from the yarrowia lipolytica; the Crispr-Cas9 technology isutilized for regulating the copy number of the carRA, the carRA, the GGS1 and the tHMG, and the recombinant bacteria capable of producing the high-yield beta-carotene is constructed. After the recombinant bacteria is fermented, cultured, extracted and separated, the content of the beta-carotene can reach the dry cell weight of 35 mg / g, and the bacterial strain stability is high.

The invention provides a method for improving yield of lycopene produced by utilizing Blakeslea trispora, and the method is characterized by comprising the following steps: (1) carrying out plate culture on Blakeslea trispora (+)(-) strain spore suspensions and dissolving one of prenol, 3-methyl-3-butene-1-ol, geraniol and mevalonolactone in ethanol to obtain a solution; (2) preparing a seed culture medium; (3) separately adding plate-cultured Blakeslea trispora (+)(-) strains to a triangular flask containing the seed culture medium; and (4) preparing a fermentation medium, mixing 1 bottle ofcultured (+) strain seed liquid and 4 bottles of cultured (-) strain seed liquid, adding the fermentation culture medium to the seed liquid for starting fermentation, then adding the solution of one of prenol, 3-methyl-3-butene-1-ol, geraniol and mevalonolactone 0-36 hours after the start of the fermentation and then carrying out constant-temperature culture. The method provided by the invention is simple and easy to operate and control, substantially improves the yield of lycopene and lowers production cost.

The invention provides a microcapsule or fatty powder containing beta-carotene. The beta-carotene is obtained through fermentation of Blakeslea trispora mutant strains. The Blakeslea trispora mutant strains are characterized in that the strains are preserved in China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, Hubei Province, China on August 8th, 2014 with accession numbers of Blakeslea trispora BT7251 (+) CCTCC M 2014378 and Blakeslea trispora BT7603 (-) CCTCC M 2014379. After the Blakeslea trispora mutant strains provided by the invention are subjected to conventional fermentation culture, high-yield beta-carotene can be obtained. Moreover, the whole fermentation process is simple, convenient to control and beneficial for industrial production.

The invention discloses a method for producing lycopene by bloking the metabolic pathway of Dunaliella bardawil. The method comprises the following steps: preparing a Dunaliella bardawil basic culturing solution, adding an A5 trace element solution to the culturing solution, adjusting the pH value, disinfecting, and cooling to room temperature; and 2, adding Dunaliella bardawil cells to the culturing solution, culturing in an illumination incubator, adding 200-800ppm of a blocker triethylamine when OD600 reaches 0.7, continuously culturing for 24-72h, and extracting a pigment to obtain lycopene. Compared with traditional methods for extracting lycopene from tomato skins, the extraction method disclosed in the invention has the advantages of simplicity, convenience, fastness and short extraction period. Compared with Blakeslea trispora and other autotrophic microorganisms, Dunaliella bardawil has the advantages of large-scale culture benefiting, simple requirements of environment required by culturing, and according with industrial large-scale production standards.

The invention provides a method or highly yielding lycopene by using Blakeslea trispora fermentation. The method comprises the steps of primary seed culture, secondary seed culture, fermenting culture, and extraction and separation. The method is characterized in that rapeseed oil is added to a fermenting culture medium to make the mass content of the rapeseed oil in the fermenting culture medium reach 2.0-5.0%, fermenting culture is carried out for 20-26h, and then nicotine accounting for 0.05-0.07% of the mass of a fermentation substance is added. Compared with the prior art, the method provided by the invention has the advantages of 10% or above increase of the lycopene yield, short production period, low cost of the fermenting culture medium, and production cost reduction.

The invention relates to Blakeslea trispora (B. trispora) strains producing at least 0.3 g / l lycopene, when co-cultivated for at least three days with a suitable strain of Blakeslea trispora of the opposite mating type in a suitable medium in the absence of an exogenous cartenogenesis inhibitor, to a natural efficient production process of lycopene by these strains, to a growth medium highly suited for this process and to an isolation process suited for any carotenoid compound.

The invention discloses blakeslea trispora, which is preserved in China General Microbiological Culture Collection Center. A method for preparing lycopene from blakeslea trispora comprises the following steps of performing activation: inoculating activation strains onto a seed culture medium to be cultured for 42 to 44 under the conditions of the temperature being 30 to 35 DEG C and the rotating speed being 150 to 220rpm for obtaining a seed culture solution; inoculating the seed culture solution onto a fermentation culture medium to be fermented for 120 to 144h at 32 to 35 DEG C under the light shielding condition; then, performing fermentation for 46 to 48h; adding a blocking agent; extracting the lycopene in the fermentation solution, wherein an extraction agent is acetone or petroleum ether, the mass ratio of the fermentation solution to the extraction agent is 1: (200 to 250), the extraction temperature is 40 to 60 DEG C, and the extraction time is 2h; extracting the extraction solution at the extraction temperature being 40 to 45 DEG C; performing overnight crystallization at -4 DEG C. Through being measured by liquid phase chromatography, the purity of the lycopene is 85 percent or higher than 85 percent. The method has the advantages that the yield of the lycopene is improved; the purity of the separated lycopene is high.

The invention discloses a method of producing beta-carotene by a genetically engineered bacterium and the genetically engineered bacterium thereof. The genetically engineered bacterium is a yarrowia lipotica genetically engineered bacterium comprising a carRA gene and a carB gene. By introducing the carRA gene and the carB gene originated from blakeslea trispora into the yarrowia lipotica yeast bymeans of a genetic engineering method, expression of GGS and 3-hydroxyl-3-methyl acetyl coenzyme A reductase is further enhanced. 3-phosphoglycerol dehydrogenase (Gut2) and peroxidase (Pox2) are knocked out to obtain a yarrowia lipotica strain expressing beta-carotene heterologously. The method is simple in fermenting condition and relatively high in output of beta-carotene, and has important production value.

The invention belongs to the field of production of lycopene and discloses a fermentation medium, the application thereof and a method for preparing lycopene through fermentation of Blakeslea trispora. The fermentation medium comprises the following ingredients: a starch component, a bean flour component, fish meal, vegetable oil, copper sulfate, magnesium sulfate, monopotassium phosphate, vitamin E and an emulsifying agent, wherein the starch component comprises starch and / or starch hydrolysate; the bean flour component comprises bean flour and / or bean flour hydrolysate; and at least one of the starch component and the bean flour component is the hydrolysate of the starch or bean flour. With the adoption of the fermentation medium provided by the invention, the concentration of dissolved oxygen can be improved, and the dissolved oxygen can be reasonably and efficiently utilized. Therefore, the yield of lycopene is improved.

The present invention relates to a method for inducing a Blakeslea trispora aging strain to rapidly produce a large number of spores. The method comprises: (1) loading a semi-solid culture medium into a culture dish, inoculating the hypha of a Blakeslea trispora aging strain into the semi-solid culture medium, culturing for 24-48 h at a temperature of 25-30 DEG C in a dark environment with a relative humidity of 80-95% and an air flow rate of 0.5 L / min, then culturing for 12-24 h at a temperature of 10-20 DEG C, and growing spores or hyphae on the culture medium flat plate; (2) collecting and streaking the obtained spores or hyphae on another new semi-solid culture medium flat plate, and culturing for the same time as the step (1) in the same environment as the step (1); and (3) repeatedly performing the operations in the step (2), culturing 1-5 generations of the hyphae, and picking or collecting the spores so as to obtain a large number of the spores. According to the present invention, with the method, the spore yield is high, the spore production is rapid, and the problem that no spore or less spores is produced during the Blakeslea trispora (especially negative bacteria) culture process is effectively solved.

The invention discloses a method for preparing beta-carotene and a beta-carotene product. According to the method for preparing beta-carotene disclosed by the invention, a bio-enzyme is added into fermentation liquor containing blakeslea trispora positive bacteria and blakeslea trispora negative bacteria in the fermentation culture process so as to perform enzymolysis treatment, wherein the bio-enzyme is selected from any one or a combination of several in pectinase, cellulase, neutral protease and lipase. Due to the effect of the bio-enzyme, pectin substances, proteins, oil and other substances adhered among mycelia are subjected to enzymolysis, so that the effects of reducing the viscosity of the fermentation liquor, improving the mycelium distribution degree and effectively improving the dissolved oxygen in the fermentation liquor are achieved, and the aims of improving the yield of beta-carotene and improving the product quality are further achieved.

The invention discloses a lycopene high-yielding strain and application thereof. The strain is classified and named as blakeslea trispora V-6515 and has been preserved in China Center for Type Culture Collection CCTCC with an accession number of CCTCC No. M 2013583. According to the invention, nitrosoguanidine mutagenesis and flask fermentation are carried out to screen a stain with high yield of lycopene, the strain is used as an original strain in the next round of mutagenesis, and the target strain V-6515 is finally screened; the strain can greatly increase lycopene in fermentation products while reducing other components and has good genetic stability. The strain employs cassava powder as a carbon source for fermentation, and yield of lycopene produced through fermentation in a 5 L fermentor can reach 1.82 g / L, increased by 23.4% compared with the original strain VR-5(-); and the strain can be used in industrial production, greatly improves a fermentation unit and has a great economic value.

The invention relates to a yellow lyocell fabric prepared by dyeing with a blakeslea trispora fermentation extract and a preparation method thereof. The method comprises the following steps of fermenting blakeslea trispora, culturing and purifying to obtain beta-carotenoid; and preparing a phenol oxidase solution and a cellulase solution, preparing a dyeing solution from the beta-carotenoid, the phenol oxidase solution, the cellulase solution, an H2O2 solution and the like, putting manganese dioxide, a lyocell fabric and a dyeing solution into a dyeing machine for dyeing, performing enzyme deactivation on the dyed lyocell fabric, washing with tap water, and drying to obtain the yellow lyocell fabric. According to the yellow lyocell fabric prepared by dyeing with the blakeslea trispora fermentation extract and the preparation method thereof provided by the invention, cellulose and the beta-carotenoid are respectively activated by selecting cellulase and polyphenol oxidase, so that graft polymerization reaction of the cellulose and the beta-carotenoid is realized. The dyeing conditions are mild, so that the pollution to the environment is small; and the dyed yellow lyocell fabric has a relatively high K / S value, and the soaping fastness and the dry rubbing fastness grade are relatively high.

The invention relates to a method for producing carotenoid through solid fermentation of blakeslea trispora, a solid fermentation extract and animal feed. The method comprises the following steps: inoculating blakeslea trispora on a fermentation culture medium and carrying out fermentation culture; and to be specific, the fermentation culture medium contains kitchen solid waste accounting for 5-30% of the total weight of the culture medium and kitchen waste grease accounting for 5-15% of the total weight of the culture medium. The preparation method of the kitchen waste solid waste and the kitchen waste grease comprises the following steps: (1), roughly selecting the kitchen waste; (2), filtering or centrifugally separating the roughly selected kitchen waste to obtain the kitchen solid waste and the kitchen liquid waste; and (3) further carrying out oil-water separation on the kitchen liquid waste to obtain the kitchen waste grease and the kitchen waste swill. Kitchen waste is used asa fermentation medium, so that the cost is low; the kitchen waste is used as a main raw material and carotenoid is generated through biotransformation, so that kitchen waste pollution is reduced whilethe feed application requirement is met.

The invention relates to a method for producing lycopene through fermentation of blakeslea trispora and lycopene, and belongs to the field of fermentation engineering. The method comprises the following steps: respectively culturing positive fungi and negative fungi of the blakeslea trispora, and taking a part of cultured positive fungi and negative fungi of the blakeslea trispora and simultaneously carrying out inoculation; performing fermentation, controlling a pH value of a fermentation system to reach 5.8 to 6.2 by using a regulating agent in a fermentation process, supplementing materialsin a period of 40 to 100 hours after beginning of fermentation, continuously fermenting to 120 hours, and then releasing from a tank, wherein the regulating agent is an acidic substance which contains PO4<3+> and does not contain metallic ions or salt; a fermentation culture medium contains starch phosphate ester substances. The method has the advantages of simpleness and low cost; a fermentationpH value is controlled by the acidic substance which contains PO4<3+> and does not contain metallic ions or salt and PO4<3+> is provided, so that introduction of excessive amount of K<+> is avoided;through cooperative application of the starch phosphate ester substances, the output of the lycopene is increased; therefore, the output of the lycopene is higher, and the method is suitable for industrial production.

The invention discloses a method of preparing lycopene and a lycopene product. The method of preparing lycopene, disclosed by the invention, comprises the step of adding a bio-enzyme and a blocking agent into fermenting liquor containing positive blakeslea trispora and positive blakeslea trispora in a fermenting culture process for enzymolysis, wherein the bio-enzyme is selected from one or combination of more of pectinase, cellulase, neutral protease and lipase. By means of action of the bio-enzyme, enzymolysis is performed on adhered substances such as pectic substance, proteins and lipid among hyphae, so that the aims of reducing the viscosity of the fermenting liquor, improving the distributing degree of hyphae, and increasing dissolved oxygen in the fermenting liquor effectively so as to improve the output of the lycopene and improve the product quality are achieved.

The invention relates to the field of bioengineering, and particularly relates to a method for preparing beta-carotin by solid fermentation by using Blakeslea trispora. The method is characterized by comprising the following steps: carrying out culture fermentation by using a solid fermentation culture medium instead of a liquid fermentation culture medium in the traditional technique, directly drying the mycelia-containing solid fermentation product under reduced pressure, and extracting the beta-carotin. Compared with the liquid fermentation, the method reduces the step of filtration or centrifugation for obtaining mycelia, greatly reduces the water consumption and wastewater discharge in the technique, is beneficial to protecting the limited fresh water resource in China, and is especially suitable for popularization and application in water-deficient areas in Northern China.

The invention relates to a method for producing beta-carotene through fermentation of blakeslea trispora, and the beta-carotene, and belongs to the field of fermentation engineering. The method comprises the steps of respectively culturing blakeslea trispora positive bacteria and blakeslea trispora negative bacteria, simultaneously inoculating, fermenting, controlling a pH value of a fermentationsystem to be 5.8 to 6.2 through a modifier during a fermenting process, semi-continuously culturing, emptying when the fermenting starts for 60h, and supplementing materials. The modifier is an acidicmaterial which contains PO4<3+> and does not contain metal ions and salt. A fermentation medium adopted in the fermenting process contains a starch phosphate material. The method is simple and low-cost; through using the acidic material which contains the PO4<3+> and does not contain the metal ions and the salt for controlling a fermenting pH value and providing the PO4<3+>, excessive K<+> is prevented from being brought in; by the cooperative use of the starch phosphate material, the yield of the beta-carotene is improved. Therefore, the obtain beta-carotene is higher in yield, and the method is suitable for industrial production.