Method for producing lycopene through fermentation of blakeslea trispora and lycopene

Abstract

The invention relates to a method for producing lycopene through fermentation of blakeslea trispora and lycopene, and belongs to the field of fermentation engineering. The method comprises the following steps: respectively culturing positive fungi and negative fungi of the blakeslea trispora, and taking a part of cultured positive fungi and negative fungi of the blakeslea trispora and simultaneously carrying out inoculation; performing fermentation, controlling a pH value of a fermentation system to reach 5.8 to 6.2 by using a regulating agent in a fermentation process, supplementing materialsin a period of 40 to 100 hours after beginning of fermentation, continuously fermenting to 120 hours, and then releasing from a tank, wherein the regulating agent is an acidic substance which contains PO4<3+> and does not contain metallic ions or salt; a fermentation culture medium contains starch phosphate ester substances. The method has the advantages of simpleness and low cost; a fermentationpH value is controlled by the acidic substance which contains PO4<3+> and does not contain metallic ions or salt and PO4<3+> is provided, so that introduction of excessive amount of K<+> is avoided;through cooperative application of the starch phosphate ester substances, the output of the lycopene is increased; therefore, the output of the lycopene is higher, and the method is suitable for industrial production.

Description

technical field [0001] The invention relates to the field of fermentation engineering, and in particular to a method for producing lycopene by B. trispora fermentation and lycopene. Background technique [0002] Existing fermentation methods usually use KH 2 PO 4 or K 2 HPO 4 Contain PO 4 3+ salt to increase phosphorylation levels and provide K + , enhance the permeability of cell membrane, and benefit the entry of nutrients into cells. Use KH 2 PO 4 or K 2 HPO 4 Contain PO 4 3+ If the amount of salt is too small, the phosphorylation level will be insufficient, which will greatly reduce the metabolic flux of carotenoids, and if it is too much, it will bring a large amount of K + , so that the cell membrane permeability of the bacteria becomes too strong, resulting in the precipitation of internal substances such as the target product lycopene into the fermentation clear night. [0003] At the same time, due to the need to add an alkaline blocking agent during t...

AI Technical Summary

Problems solved by technology

Use KH 2 PO 4 or K 2 HPO 4 Contain PO 4 3+ If the amount of salt is too small, the phosphorylation level will be insufficient, which will greatly reduce the metabolic flux of carotenoids, and if it is too much, it will bring a large amount of K + , so that the cell membrane permeability of the bacteria becomes too strong, resulting in the precipitation of internal substances such as the target product lycopene into the fermentation clear night

[0003] At the same time, due to the need to add an alkaline blocking agent during the fermentation process, it inhibits the production of the acidic hormone trisporic acid by B. trispora, which further leads to the weakening of the metabolic flux of lycopene. precursor substances such as β-ionone, etc., but the added precursor substances are usually expensive, or difficult to obtain from the market in large quantities, these are the defects of its application in industrial production

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