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Method for promoting lycopene synthesis in blakeslea trispora

A technology based on brucella and lycopene, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of cumbersome and lengthy extraction process, limited product application range, high cost, etc.

Active Publication Date: 2013-09-04
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The planting of the raw materials required by the plant extraction method is seasonal, and the yield content is controlled by many factors. The content of lycopene in plants is unstable, and the raw materials often contain other carotenoids, so the extraction process is cumbersome and lengthy, and the cost is expensive. Although the synthetic method has a lower cost, due to environmental pollution and low product activity, there is a certain gap with natural lycopene, and the scope of product application is limited. Therefore, the use of microbial fermentation to produce lycopene has attracted widespread attention from scholars at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] 1) Plate culture: Take 20g of peeled potatoes, add 200ml of deionized water and boil for 20min. After cooling, filter with four layers of gauze, take the filtered supernatant and add 2g glucose and 2g agar. Sterilize at 115°C for 30 min in a high-pressure steam sterilizer to prepare PDA medium. Spore suspensions of (+) and (-) strains of Blakeslea trispora were spread on PDA plates respectively, cultured in a constant temperature incubator at 28°C for 4 days, and stored at 4°C.

[0014] 2) Preparation of seed medium: Add 40g of cornstarch, 20g of glucose, and 50g of corn steep liquor into a 2-liter beaker, dilute to 1000ml with deionized water, and gelatinize at 95°C for 40min. After cooling, add 1.5g potassium dihydrogen phosphate, 0.1g magnesium sulfate heptahydrate, 0.01g vitamin B 1 , after mixing, adjust the pH to 6.5 with 3mol / l sodium hydroxide solution, dispense into 250ml Erlenmeyer flasks, and sterilize in a high-pressure steam sterilizer at 115°C for 30 min...

Embodiment 2

[0024] 1) Plate culture: Take 20g of peeled potatoes, add 200ml of deionized water and boil for 20min. After cooling, filter with four layers of gauze, take the filtered supernatant and add 2g glucose and 2g agar. Sterilize at 115°C for 30 min in a high-pressure steam sterilizer to prepare PDA medium. Spore suspensions of Blakeslea trispora (+) and (-) strains were spread on PDA plates respectively, cultured in a constant temperature incubator at 28°C for 3 days, and stored at 4°C.

[0025] 2) Preparation of seed medium: Add 40g of cornstarch, 20g of glucose, and 50g of corn steep liquor into a 2-liter beaker, dilute to 1000ml with deionized water, and gelatinize at 95°C for 40min. After cooling, add 1.5g potassium dihydrogen phosphate, 0.1g magnesium sulfate heptahydrate, 0.01g vitamin B 1 , after mixing, adjust the pH to 6.5 with 3mol / l sodium hydroxide solution, dispense into 250ml Erlenmeyer flasks, and sterilize in a high-pressure steam sterilizer at 115°C for 30 minute...

Embodiment 3

[0035] 1) Plate culture: Take 20g of peeled potatoes, add 200ml of deionized water and boil for 20min. After cooling, filter with four layers of gauze, take the filtered supernatant and add 2g glucose and 2g agar. Sterilize at 115°C for 30 min in a high-pressure steam sterilizer to prepare PDA medium. Spore suspensions of Blakeslea trispora (+) and (-) strains were spread on PDA plates respectively, cultured in a constant temperature incubator at 28°C for 5 days, and stored at 4°C.

[0036] 2) Preparation of seed medium: Add 40g of cornstarch, 20g of glucose, and 50g of corn steep liquor into a 2-liter beaker, dilute to 1000ml with deionized water, and gelatinize at 95°C for 40min. After cooling, add 1.5g potassium dihydrogen phosphate, 0.1g magnesium sulfate heptahydrate, 0.01g vitamin B 1 , after mixing, adjust the pH to 6.5 with 3mol / l sodium hydroxide solution, dispense into 250ml Erlenmeyer flasks, and sterilize in a high-pressure steam sterilizer at 115°C for 30 minute...

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Abstract

The invention relates to a method for promoting lycopene biosynthesis in blakeslea trispora. The method comprises the following steps of: (1) respectively culturing blakeslea trispora (Blakeslea trispora) (+) (-) strains, and obtaining spore suspension; (2) respectively inoculating the blakeslea trispora (Blakeslea trispora) (+) (-) strains to conical flasks filled with a seed culture medium; (3) uniformly mixing the cultured positive bacteria seed solution in one bottle and negative bacteria seed solution in four bottles, and inoculating the seed solutions into a fermentation medium for fermenting; and (4) adding NAD<+> precursor substances within 24-48 hours after the beginning of the fermentation, and continuously culturing for 84 hours. The method is easy to operate, the yield of the lycopene is greatly improved, the production cost is reduced, and the method can be used for industrial production of lycopene.

Description

technical field [0001] The invention belongs to the field of improving the biosynthesis of secondary metabolites of submerged fermentation of aerobic microbial liquid, and particularly relates to a method for adding NAD + A method by which precursor substances promote lycopene biosynthesis in B. trispora. Background technique [0002] Lycopene is a fat-soluble pigment and one of the main carotenoids found in human plasma. It has the functions of efficiently quenching singlet oxygen and scavenging free radicals. Its antioxidant activity is the strongest among carotenoids. Lycopene plays an important role in preventing the occurrence of certain cancers and chronic diseases in humans. It is currently the most functional carotenoid in the world. A hotspot in food composition research. [0003] For the production method of lycopene, in addition to extraction from plants and chemical synthesis, lycopene can also be produced by microbial fermentation. The planting of the raw mat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P5/02C12R1/645
Inventor 袁其朋胡仙妹
Owner BEIJING UNIV OF CHEM TECH
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