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Method for producing lycopene by fermentation of blakeslea trispora

A technology of Brassella mold and fermentation method, which is applied in the field of industrialized large-scale production of lycopene, and can solve the problems of high heavy metal residues, carcinogenicity, toxic and side effects, etc.

Inactive Publication Date: 2010-09-22
XINJIANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the products produced by this technology have high residues of solvents and heavy metals, and when consumed in excess, they have toxic side effects on the human body and can cause cancer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1, the tank-batch fermentation method based on the biological characteristic of three spora boulardii

[0017] The tank-batch fermentation method based on the biological characteristics of Brassella trispora includes eggplant bottle slant culture of mother species, 15L-150L-1500L seed tank expansion culture of seed solution, and positive and negative bacteria 1:10 mixed 10m 3 There are several stages of fermentation in the fermenter. After eggplant bottle constant temperature cultivation, temperature stimulation to maximize sporulation, use a sterile inoculation loop to assist physiological saline to elute the spore suspension and inoculate it in the first-level seed tank. After the mycelium is physiologically mature, the sporulation is maximized, and then transplanted Gradually expand cultivation, mixed species fermentation culture.

[0018] (1) Eggplant bottle slant culture: use a sterile inoculation loop to assist 10mL sterile water to elute the spores, t...

Embodiment 2

[0021] Example 2, the post-extraction method based on the physicochemical properties of lycopene

[0022] The post-extraction method based on the physical and chemical properties of lycopene includes several stages of pretreatment of fermentation products, extraction of lycopene, concentration, crystallization, and desolventization. After tank-batch fermentation, the fermented product is pretreated by centrifugal dehydration, ball milling, etc., and put into the extraction tank. After three full cycles of extraction, it undergoes decompression concentration, low-temperature crystallization, and three-step precipitation to obtain lycopene oil. Resin, in preparation for the deployment of 6% and 10% content target products.

[0023] (1), pretreatment of fermentation products

[0024] Put 50 L of the fermentation product obtained through tank-batch fermentation into a 200-mesh filter bag and place it in a SS800 three-legged centrifuge, centrifuge at a speed of 1200 r / min for 30 min...

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Abstract

The method provides a method for tank batch fermentation based on biological characteristics of blakeslea trispora. The method comprises eggplant bottle slant culture of mother seeds, 15L-150L-1,500L seed tank amplification culture of seed liquid, and 10m<3> fermentation tank fermentation culture in a mixed ratio of positive bacteria to negative bacteria of 1:10. Preferably, the tank batch fermentation step of the lycopene also includes an inoculation mode of eluting sporangium with aseptic normal saline and sending the sporangium to a first-class seed culture medium, and after fermentation, the lycopene yield is 800mg / L. The invention also provides a late extraction method based on physical-chemical characteristics of the lycopene, and the method comprises the steps of: pretreatment of fermentation products, extraction of the lycopene, concentration of the lycopene, crystallization of the lycopene, precipitation of the lycopene and the like. Preferably, the late extraction step of the lycopene also includes a step of triple circulating extractions and three-step precipitation, and after triple circulating extractions, the extraction ratio of the lycopene is over 85 percent, and after the three-step precipitation, the residual amount of solvents can be controlled in 50ppm.

Description

technical field [0001] The invention relates to a method for producing lycopene by microbial fermentation, in particular to a method for realizing the industrialized large-scale production of lycopene through liquid submerged fermentation based on the biological characteristics of B. trispora. Background technique [0002] Lycopene is a fat-soluble unsaturated hydrocarbon, a kind of carotenoid, and its molecular formula is C 40 h 56 , with a relative molecular mass of 536.85, a melting point of 174°C (trans), and β-carotene are isomers. Lycopene is an intermediate in the biosynthesis of many carotenoids. It can be cyclized to form other carotenoids, but it cannot be synthesized in animals and can only be ingested from food. Lycopene is a carotenoid with superior functions. Because of its excellent physiological functions, it has become one of the hot spots in the field of medical and health research. In nature, lycopene mainly exists in fruits and vegetables such as tomat...

Claims

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Application Information

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IPC IPC(8): C12P5/02C07C11/21C07C7/00C12R1/645
CPCY02E50/343Y02E50/30
Inventor 陈恒雷崔俊章雷鸣王涛涛崔正禄
Owner XINJIANG UNIVERSITY
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