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Method for highly yielding lycopene by using Blakeslea trispora fermentation

A bollidium and lycopene technology, applied in the directions of microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems of high cost, low lycopene yield, etc., achieve low cost, reduce production cost, The effect of increasing content and yield

Active Publication Date: 2016-10-26
云南泽维制药有限公司
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AI Technical Summary

Problems solved by technology

However, in the existing method of producing lycopene by fermentation of B. trispora, the yield of lycopene is low and the cost is high.

Method used

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  • Method for highly yielding lycopene by using Blakeslea trispora fermentation

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Embodiment Construction

[0011] Below in conjunction with embodiment the present invention is further described.

[0012] The present invention comprises primary seed cultivation, secondary seed cultivation, fermentation cultivation, extraction and separation steps, and its specific method is:

[0013] 1. Primary seed culture: Inoculate one slant of B. trispora positive bacteria and 7 slants of negative bacteria into the primary seed tank culture medium respectively, at 27°C±1°C, 150r / min, control Dissolved oxygen > 20%, cultivated for 40 hours under the condition of air volume 1-2vvm, until the mycelium of positive bacteria is light yellow, and the mycelium of negative bacteria is bright yellow, all of which have a certain viscosity.

[0014] 2. Secondary seed culture: Inoculate 20L of positive bacteria and 140L of negative bacteria from the first-level seed liquid of B. trispora described in 1 into respective secondary seed tank medium.

[0015] 3. Fermentation culture: mix the mature positive and ...

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Abstract

The invention provides a method or highly yielding lycopene by using Blakeslea trispora fermentation. The method comprises the steps of primary seed culture, secondary seed culture, fermenting culture, and extraction and separation. The method is characterized in that rapeseed oil is added to a fermenting culture medium to make the mass content of the rapeseed oil in the fermenting culture medium reach 2.0-5.0%, fermenting culture is carried out for 20-26h, and then nicotine accounting for 0.05-0.07% of the mass of a fermentation substance is added. Compared with the prior art, the method provided by the invention has the advantages of 10% or above increase of the lycopene yield, short production period, low cost of the fermenting culture medium, and production cost reduction.

Description

technical field [0001] The invention relates to the field of industrial fermentation of lycopene, in particular to a method for increasing the yield of lycopene by fermenting B. trispora. Background technique [0002] Lycopene is a natural pigment contained in plants, mainly in the ripe fruit of tomato, a plant of the family Solanaceae. It is an intermediate in the biosynthesis of many carotenoids. Its antioxidant properties are the strongest among all carotenoids, and its ability to scavenge singlet oxygen is 100 times that of vitamin E, a commonly used antioxidant, and more than 2 times that of β-carotene; lycopene has superior physiological functions , it not only has the effect of anti-cancer and tumor suppression, but also has great significance for preventing various adult diseases such as cardiovascular disease and arteriosclerosis, enhancing the human immune system and delaying aging. It is a promising new functional Natural colouring. [0003] At present, lycopen...

Claims

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Application Information

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IPC IPC(8): C12P5/02C12R1/645
CPCC12P5/026
Inventor 杨毅张小元杨宗禄张燕杨晓亮邓波
Owner 云南泽维制药有限公司
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