Method for improving the yield of lycopene by enhancing growth of Blakeslea trispora

A technology based on brucella and lycopene, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as difficulty in oxygen transfer, high viscosity, and difficulty in industrial production

Inactive Publication Date: 2009-10-14
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since B. trispora is a highly aerobic bacterium, the demand for oxygen in the fermentation process is very large. The production medium is a gelatinized starch slurry with a very high viscosity, which brings difficulties to the oxygen transfer in the fermentation process. It is extremely difficult. In the domestic patent CN1582328A, the oxygen demand in the growth process of the bacteria is satisfied by increasing the blast and stirring speed, but this method needs to consume a large amount of energy, which increases the production cost and causes difficulties for industrial production.
It has been reported that adding liquid alkanes to the fermentation broth can increase the dissolved oxygen content, but the added concentration should be as small as possible to avoid damage to the bacteria, and the separation and extraction of the later products must undergo detoxification treatment, which is cumbersome to operate

Method used

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  • Method for improving the yield of lycopene by enhancing growth of Blakeslea trispora
  • Method for improving the yield of lycopene by enhancing growth of Blakeslea trispora

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Embodiment 1

[0025] Embodiment 1: ① plate culture: take 20 g of peeled potatoes, add 200 ml of deionized water to boil, filter the potatoes after cooling, add 2 g of glucose and 2 g of agar to the supernatant, dissolve and subpackage and sterilize at 115 ° C for 30 minutes to prepare PDA Medium. The spore suspensions of B. trispora (+) (-) strains were respectively spread on plates containing PDA medium, and cultured at 28° C. in the dark for 3 days and at 22° C. for 2 days. ② Activated carbon pretreatment: Filter the activated carbon through 200 mesh, 100 mesh, 60 mesh, 16 mesh, and 5 mesh sieves to screen out activated carbon with different particle sizes, soak them in hydrochloric acid with a mass percentage concentration of 0.5% for 2 hours, and suction filter , washed with deionized water until neutral, and dried at 40°C for later use. ③Preparation of seed medium: weigh 40g of starch, 20g of glucose, 50g of corn steep liquor, KH 2 PO 4 1.5g, MgSO 4 0.1g, dissolve in 1 liter of de...

Embodiment 2

[0030] Embodiment 2: ① plate culture: take 20 g of peeled potatoes, add 200 ml of deionized water to boil, filter the potatoes after cooling, add 2 g of glucose and 2 g of agar to the supernatant, dissolve and subpackage for sterilization at 115 ° C for 30 minutes to prepare PDA Medium. The spore suspension of B. trispora (+) (-) strains was spread on a plate containing PDA medium, and cultured at 28° C. in the dark for 3 days and at 22° C. for 2 days. ②Activated carbon pretreatment: Soak 100-mesh activated carbon in hydrochloric acid with a concentration of 0.7% by mass for 2 hours, filter with suction, wash with deionized water until neutral, and dry at 40°C for later use. ③Preparation of seed medium: weigh 40g of starch, 20g of glucose, 50g of corn steep liquor, KH 2 PO 4 1.5g, MgSO 4 0.1g, was dissolved in 1 liter of deionized water, heated and stirred to gelatinize until it became viscous, and the pH value was adjusted to 6.4. Dispense into 250ml Erlenmeyer flasks a...

Embodiment 3

[0035] Embodiment 3: 1. plate culture: take 20 g of peeled potatoes, add 200 ml of deionized water to boil, filter the potatoes after cooling, add 2 g of glucose and 2 g of agar to the supernatant, dissolve and subpackage for sterilization at 115 ° C for 30 minutes to prepare PDA Medium. The spore suspension of B. trispora (+) (-) strains was spread on a plate containing PDA medium, and cultured at 28° C. in the dark for 3 days and at 22° C. for 2 days. ②Activated carbon pretreatment: Soak 100-mesh activated carbon in hydrochloric acid with a concentration of 1.0% by mass for 2 hours, filter with suction, wash with deionized water until neutral, and dry at 40°C for later use. ③Preparation of seed medium: weigh 30g of starch, 15g of glucose, 45g of corn steep liquor, KH 2 PO 4 1.5g, MgSO 4 0.1g, dissolve in 1 liter of deionized water, heat and stir to gelatinize until it becomes viscous, and adjust the pH value to 6.4. Dispense into 250ml Erlenmeyer flasks and sterilize a...

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Abstract

The invention relates to a method for improving the yield of lycopene by enhancing the growth of Blakeslea trispora, belonging to the field of improving liquid-submerged fermentation of aerobic microorganisms to produce secondary metabolite, in particular to a method for adding active carbon to enhance Blakeslea trispora to produce lycopene. The method comprises the following steps of: adding active carbon in a fermentation liquor, and utilizing the characteristic that active carbon absorbs gas to increase the concentration of dissolved oxygen in the fermentation liquor, improve the viscosity of the fermentation liquor, enhance the growth of Blakeslea trispora and improve the yield of lycopene by more than 150 percent than the original yield. The method has low cost, convenient operation and obvious effect, can replace liquid alkane and hydrogen peroxide as an oxygen-carrying agent, avoids toxicity of chemical substances to the strains, simplifies post purification process of the lycopene and provides good basis for industrial production.

Description

technical field [0001] The invention belongs to the field of improving secondary metabolites of liquid submerged fermentation aerobic microorganisms, and in particular relates to a method for increasing the lycopene output of B. trispora by adding activated carbon. Background technique [0002] Lycopene is an important carotenoid with a strong antioxidant effect. It is the carotenoid with the strongest antioxidant capacity. It has a strong function of scavenging singlet oxygen and protects the body cells from oxidative damage. Its physiological activity can effectively prevent and treat prostate cancer, gastric cancer, skin cancer, etc., and its inhibitory effect on uterine cancer and lung cancer cells is significantly higher than that of β-carotene and α-carotene. [0003] Lycopene can be extracted from tomato skin, synthesized by chemical methods, or produced by microbial fermentation. Considering the obtained product quality, production technology and resource cost, the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P5/02C12R1/645
Inventor 袁其朋刘淑惠
Owner BEIJING UNIV OF CHEM TECH
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