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Beta-carotene-containing bacterial powder, microbial oil and oil suspension

A technology of carotene and bacterial powder, applied in the direction of microorganisms, microorganisms, methods based on microorganisms, etc., can solve the problem of low yield of β-carotene, and achieve the effect of simple fermentation process, high yield, and favorable industrial production

Inactive Publication Date: 2016-08-17
CABIO BIOTECH WUHAN CO LTD
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Problems solved by technology

However, the yield of β-carotene produced by using this strain is low, and the highest yield does not exceed 1.3g / L

Method used

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  • Beta-carotene-containing bacterial powder, microbial oil and oil suspension
  • Beta-carotene-containing bacterial powder, microbial oil and oil suspension

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Embodiment Construction

[0010] In order to understand the technical content of the present invention more clearly, the following examples are given in detail.

[0011] Breeding method of mutant strain of B. trispora

[0012] 1) Commercially available B. trispora positive bacteria (preservation number: CCTCCAF97006(+)) and B. trispora negative bacteria (preservation number: CCTCCAF96002(-)) were used as starting strains.

[0013] 2) The two starting strains were cultured for 5 days until the spores matured.

[0014] 3) Filter through gauze or filter paper to obtain pure spore liquid, inject the spore liquid into a sterile petri dish, and dry it with sterile air to become bacterial plaque. Aseptically move the petri dish containing bacterial plaque into a high-energy particle beam implanter, and undergo mutagenesis by high-energy ion beam implantation.

[0015] 4) The bacterial plaques of the positive and negative strains were eluted with sterile water respectively, and the obtained positive and neg...

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Abstract

The invention provides beta-carotene-containing bacterial powder or microbial oil or oil suspension. Beta-carotene is obtained by fermenting a blakeslea trispora mutant strain. The beta-carotene-containing bacterial powder or microbial oil or oil suspension is characterized in that the blakeslea trispora mutant strain is preserved in the China Center For Type Culture Collection (CCTCC) on August 8, 2014, the preservation address is Wuhan University, Wuhan city, China, and the preservation numbers are blakeslea trispora BT7251(+) CCTCC M 2014378 and blakeslea trispora BT7603(-) CCTCC M 2014379. The beta-carotene with the high yield can be obtained by conventionally fermenting and culturing the blakeslea trispora mutant strain. Meanwhile, the whole fermentation technology is simple, convenient to control and beneficial for industrialized production.

Description

technical field [0001] The invention relates to bacteria powder, microbial oil and oil suspension containing beta-carotene. Background technique [0002] β-carotene is one of more than 500 kinds of carotene. It is the precursor of vitamin A, also known as provitamin A. Its dilute solution is orange-yellow to yellow. β-carotene widely exists in plants, algae and fungi, but animals and humans cannot synthesize carotene and must be ingested from the outside. As a food additive, β-carotene can act as a pigment and a nutritional enhancer. At the same time, β-carotene is a powerful antioxidant, which can inhibit the proliferation of cancer cells and improve the body's immunity. The unique nutritional value and medicinal efficacy make carotene highly valued by medical circles and food merchants all over the world. [0003] At present, the preparation methods of β-carotene mainly include the following types: 1. Chemical synthesis, that is, using organic chemical raw materials to a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P23/00C12R1/645
CPCA23V2002/00C12P23/00C12N1/145C12R2001/645A23V2250/211
Inventor 汪志明李翔宇余超陆姝欢周强肖敏张玉良
Owner CABIO BIOTECH WUHAN CO LTD
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