Method for preparing beta-carotene and beta-carotene product

A carotene and product technology, which is applied in the field of beta-carotene products and preparation of beta-carotene, can solve the problems of low yield of beta-carotene and the like

Active Publication Date: 2018-01-19
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing method for producing β-carotene by B. trispora fermentatively produces lower β-carotene yields

Method used

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  • Method for preparing beta-carotene and beta-carotene product
  • Method for preparing beta-carotene and beta-carotene product
  • Method for preparing beta-carotene and beta-carotene product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] A kind of method for preparing β-carotene provided by the present embodiment, it comprises the following steps:

[0076] 1 slant culture

[0077] Take the spore suspensions of the positive bacteria and the negative bacteria of B. trispora, spread them on the PDA slant medium, and cultivate them in a constant temperature incubator at 25°C for 5-7 days;

[0078] Among them, the positive bacterium of B. trispora is B. trispora BT7251 (+), and the preservation number is CCTCCM2014378; the negative bacterium of B. trispora is B. trispora BT7603 (-), and the storage number is CCTCCM2014379.

[0079] Among them, the PDA slant medium includes: glucose 20g / L, agar powder 25g / L, peeled potatoes 200g / L; its preparation method can be referred to as follows: cut potatoes into 1cm cubes, add deionized water to boil for 30 minutes, cool and use Filter with four layers of gauze, take the filtered supernatant and add glucose and agar powder.

[0080] 2 seed culture

[0081] Use an i...

Embodiment 2

[0105] The method for preparing β-carotene provided in this example is basically the same as the steps in Example 1, except that the bio-enzyme added in the fermentation process of Step 3 is pectinase (50000U / g). The test results are shown in Table 1.

Embodiment 3

[0107] The method for preparing β-carotene provided in this example is basically the same as the steps in Example 1, except that the biological enzyme added during the fermentation process in Step 3 is neutral protease (100000U / g). The test results are shown in Table 1.

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Abstract

The invention discloses a method for preparing beta-carotene and a beta-carotene product. According to the method for preparing beta-carotene disclosed by the invention, a bio-enzyme is added into fermentation liquor containing blakeslea trispora positive bacteria and blakeslea trispora negative bacteria in the fermentation culture process so as to perform enzymolysis treatment, wherein the bio-enzyme is selected from any one or a combination of several in pectinase, cellulase, neutral protease and lipase. Due to the effect of the bio-enzyme, pectin substances, proteins, oil and other substances adhered among mycelia are subjected to enzymolysis, so that the effects of reducing the viscosity of the fermentation liquor, improving the mycelium distribution degree and effectively improving the dissolved oxygen in the fermentation liquor are achieved, and the aims of improving the yield of beta-carotene and improving the product quality are further achieved.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a method for preparing β-carotene and a β-carotene product. Background technique [0002] β-carotene is a kind of fat-soluble carotenoid, which has good anti-oxidation, anti-cancer and anti-cancer effects, and has certain effects on preventing cardiovascular diseases, enhancing the body's immunity and delaying aging. It is a very A functional natural pigment with development value. [0003] The production of β-carotene raw materials mainly includes natural extraction method, chemical synthesis method and microbial fermentation method. The raw materials required by the natural extraction method are mainly carrots, tomatoes and other plants. These plants are limited by climate, place of origin, transportation and other conditions, and the plant extraction process is cumbersome and lengthy, and the cost is expensive, which cannot meet the needs of industrial product...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12R1/645
Inventor 汪志明李翔宇陆姝欢余超杨艳红
Owner CABIO BIOTECH WUHAN CO LTD
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