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Producing of novel poultry biological feed stuff and preserve method thereof

A technology of biological feed and production method, which is applied in the preparation and preservation process of new poultry biological feed, the preservation process of bacterial cell biological feed rich in β-carotene, the limited mixed fermentation technology of Brassella trispora, and the technology rich in β-carotene In the field of vegetative bacterial feed preparation, it can solve problems such as difficult to achieve skin and yolk pigment deposition, carotenoid deficiency, and failure to meet consumers' requirements for product color.

Inactive Publication Date: 2008-09-03
ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of modern large-scale, high-density, and intensive farming, the total daily intake of carotenoids in poultry decreases, often resulting in carotenoid deficiency, which makes it difficult to achieve ideal skin and egg yolk pigmentation, and cannot meet Consumer requirements for product color

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Mycelium culture

[0015] Prepare slant medium, containing 2% peptone, 1% yeast extract, 2% glucose, and 2% agar in the slant medium; get the selected Blakeslea trispora trispora (Blakeslea trispora z1+, Blakeslea trispora z1+, Blakeslea trispora z1-) was activated twice in the slant medium, the first activation condition was 28°C, 8d, and the second activation condition was 28°C, 5d. The thalline was inserted into two Erlenmeyer flasks (300mL) each containing 50mL of seed culture solution, and carried out primary seed culture (28°C for 44h). The culture medium contained 5% soybean flour, 2.5% corn flour, and VB 1 0.005%, pH 6.4 ~ 6.7; Take the first-level seeds and insert them into the second-level seed medium according to the inoculum amount of 3% for second-level seed culture (cultivate at 28°C for 42 hours), the second-level seed medium components are the same as the first-level seed medium . Then get the secondary seeds of the positive and negative bacterial ...

Embodiment 2

[0021] (1) Mycelium culture

[0022] The slant activation process is the same as that of Example 1. The activated slant thallus is washed into the wort double-layer plate with aseptic water. The lower layer of the wort double-layer plate is 2% agar, and the upper layer is 8% agar at 28°C. Cultivate for 2-3 days until the spores in the flat plate grow luxuriantly, wash the spores with sterile water to make a spore suspension (10 6 Individual / mL), the spore suspension is connected in the triangular flask (1000mL) that 500mL seed culture solution is housed by 5% inoculum size, under 28 ℃ of conditions, carry out seed expansion culture, culture medium is soybean powder 5%, corn Powder 2.5%, VB 1 0.005%, pH 6.4~6.7, take the secondary seeds of the positive and negative strains that have been cultivated for 42 hours, mix them according to the inoculation ratio of 1:1, the inoculation amount is 6%, and put them into a 1L fermenter. The ratio of the medium is: starch 41g / L, soybean ...

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Abstract

The invention belongs to the technology field of biological feed preparation technique, and provides a novel method for producing and storing poultry biological feed. The invention comprises the cultivation of first-grade seeds and second-grade seeds with blakeslea trispora, the limited and mixed fermentation and production of positive-negative bacterial strains, the vacuum dry preparation of thalli at a relatively low temperature, the spraying dry preparation of thalli, and the storage technique and parameters of products. The production and storage method, and the preparation technique is novel, and final products are economical. Through the invention, the products rich in beta-renieratene through the dry preparation at the low temperature and the spraying preparation are directly applied to the production of poultry, thereby reducing high cost caused by processing and purification, increasing yield rate, and reducing environmental pollution caused by mycelium treatment. The feed produced by the production method is rich in beta-renieratene, protein, vitamin, etc., and is helpful to increasing the laying rate and the hatchable egg rate, raising the growth rate of the broiler chicken, reducing the death rate of the broiler chicken, and deepening the color of egg yolk.

Description

Technical field: [0001] The invention belongs to the technical field of biological feed preparation technology, and in particular relates to a preparation and preservation process of a new type of poultry biological feed, in particular to a restricted mixed fermentation technology for B. trispora, a preparation technology for biological feed rich in β-carotene, And the preservation technology of bacterial cell biological feed rich in β-carotene. Background technique: [0002] β-carotene (β-carotene) is one of more than 600 kinds of carotenoids. It is the precursor of vitamin A. It is widely found in vegetables and fruits and has dual functions of nutrition and coloring. It is a class A excellent and nutritious food additive identified by the Joint Expert Committee on Food Additives. [0003] β-carotene has dual functions of nutrition and coloring, and has been widely used in food, health products and cosmetics. With the discovery of natural β-carotene's strong antioxidant ...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K3/00A23K10/12A23K20/105A23K50/75
Inventor 胡伟莲戴德慧刘士旺毛建卫陈贵才
Owner ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
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