Method for preparing beta-carotin by solid fermentation

A carotene and post-fermentation technology, applied in the field of bioengineering, can solve problems such as multiple water resources, large fermentation waste liquid, consumption, etc., and achieve the effects of less environmental pollution and reduced waste

Active Publication Date: 2015-06-10
CHINA PHARM UNIV
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  • Abstract
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Problems solved by technology

[0010] The methods used in the above patents all use liquid fermentation medium, which

Method used

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  • Method for preparing beta-carotin by solid fermentation
  • Method for preparing beta-carotin by solid fermentation

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Embodiment 1

[0034] Incline medium: PDA medium, the preparation method is 200g of peeled potatoes, chopped, add 1L of water and boil for 20min, filter with 8 layers of gauze, remove the filtrate, add 20g of glucose, 20g of agar powder, heat and melt, mix well and fill Put it into a test tube and prepare a PDA slope after sterilization.

[0035] Seed medium: Same as the slant medium, sterilized and poured into a plate to prepare a PDA plate.

[0036] Fermentation medium: 400g of millet, 50g of bran, 100g of soybean oil, 100g of Tween 80, 10g of soybean cake powder, KH 2 PO 4 0.5g, KCl 0.5g add water to 1kg. Stir the prepared medium evenly and spread it on a stainless steel tray, cover with eight layers of gauze, steam in a steamer for 10 minutes, take it out and break it into loose granules with a spatula, cover with eight layers of gauze, and sterilize at 121°C for 25 minutes .

[0037] (1) Activation of bacteria

[0038] Take out the freeze-dried tube of the preserved B. trispora (+) b...

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Abstract

The invention relates to the field of bioengineering, and particularly relates to a method for preparing beta-carotin by solid fermentation by using Blakeslea trispora. The method is characterized by comprising the following steps: carrying out culture fermentation by using a solid fermentation culture medium instead of a liquid fermentation culture medium in the traditional technique, directly drying the mycelia-containing solid fermentation product under reduced pressure, and extracting the beta-carotin. Compared with the liquid fermentation, the method reduces the step of filtration or centrifugation for obtaining mycelia, greatly reduces the water consumption and wastewater discharge in the technique, is beneficial to protecting the limited fresh water resource in China, and is especially suitable for popularization and application in water-deficient areas in Northern China.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a method for solid-state fermentation of beta-carotene by using B. trispora. Background technique [0002] The molecular formula of β-carotene is C 40 h 55 , an effective physiological antioxidant, has the functions of anti-aging, preventing and treating cardiovascular diseases, anti-tumor, enhancing the body's specific and non-specific immune function, regulating inflammatory reactions, preventing radiation, increasing the connection and communication between cells, and its As a provitamin, it can prevent night blindness caused by vitamin A deficiency, promote the normal maturation of epithelial cells, and is also an ideal food coloring agent and nutritional supplement. [0003] At present, there are three main methods for producing β-carotene, namely chemical synthesis, natural product extraction and microbial fermentation. Among them, the microbial fermentation method has the ...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12R1/645
Inventor 郑珩李凌云劳兴珍余永柱张彦成欣
Owner CHINA PHARM UNIV
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