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Method for preparing beta-carotene through fermentation of Blakeslea trispora

A technology of B. boulardii and B. trispora is applied in the field of preparing beta-carotene, can solve the problems of high cost, low yield of beta-carotene, large amount of vegetable oil added, etc., and achieves low cost and solid content. Low content, cost and resource saving effect

Active Publication Date: 2012-10-31
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the yield of β-carotene prepared by this method is too low
In addition, Chinese patent CN1193048A discloses a method for fermenting and producing β-carotene by culturing filamentous fungi in an air-lift fermenter by using spore inoculation. The process includes primary seed cultivation and secondary seed cultivation and fermentation. However, fermentation The amount of vegetable oil added to the medium is relatively large, resulting in high costs

Method used

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  • Method for preparing beta-carotene through fermentation of Blakeslea trispora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Preparation of β-carotene-rich strains by fermentation culture in shake flasks

[0024] 1. Strain:

[0025] B. trispora ATCC 14271 (+) and ATCC 14272 (-)

[0026] 2. Medium:

[0027] 1) Preparation and composition of inclined plane PDA medium: Take 200g of fresh unsprouted potatoes, peel them, cut into 3mm×3mm pieces, add 800-1000ml of water, boil for 30min, then filter with gauze, add sucrose to the filtrate ( Or glucose) 20g, 15-20g agar, replenish water to 1000ml after dissolution, natural pH, aliquot into test tubes (5ml per tube), sterilize at 121°C for 30min, and make a bevel.

[0028] 2) The seed medium formula is (W / V): corn flour 3%, soybean flour 3%, KH 2 PO 4 0.1%, MgSO 4 0.02%, V B1 0.001%, pH 6.5.

[0029] 3) Preparation method of bran extract: Take 40.8 grams of bran, add 1000mL tap water, boil for 1h, filter with double gauze, and dilute the filtrate to 1000mL to obtain the bran extract.

[0030] 4) The formula of the fermentation medium: take 10...

Embodiment 2

[0042] Example 2: Preparation of β-carotene-rich strains by fermentation culture in a 10L mechanically stirred fermentor

[0043] 1. Strain:

[0044] B. trispora ATCC 14271 (+) and ATCC 14272 (-)

[0045] 2. Medium:

[0046] 1) Preparation and composition of inclined plane PDA medium: Take 200g of fresh unsprouted potatoes, peel them, cut into 3mm×3mm pieces, add 800-1000ml of water, boil for 30min, then filter with gauze, add sucrose to the filtrate ( Or glucose) 20g, 15-20g agar, replenish water to 1000ml after dissolution, natural pH, aliquot into test tubes (5ml per tube), sterilize at 121°C for 30min, and make a bevel.

[0047] 2) The seed medium formula is (W / V): corn flour 3%, soybean flour 3%, KH 2 PO 4 0.1%, MgSO 4 0.02%, V B1 0.001%, pH 6.5.

[0048] 3) Preparation method of bran extract: Take 40.8 grams of bran, add 1000mL tap water, boil for 1h, filter with double gauze, and dilute the filtrate to 1000mL to obtain the bran extract.

[0049] 4) The formula of th...

Embodiment 3

[0061] Example 3: Preparation of β-carotene-rich strains by fermentation culture in a 100L mechanically stirred fermentor

[0062] 1. Strain:

[0063] B. trispora ATCC 14271 (+) and ATCC 14272 (-)

[0064] 2. Medium:

[0065] 1) Preparation and composition of inclined plane PDA medium: Take 200g of fresh unsprouted potatoes, peel them, cut into 3mm×3mm pieces, add 800-1000ml of water, boil for 30min, then filter with gauze, add sucrose to the filtrate ( Or glucose) 20g, 15-20g agar, replenish water to 1000ml after dissolution, natural pH, aliquot into test tubes (5ml per tube), sterilize at 121°C for 30min, and make a bevel.

[0066] 2) The seed medium formula is (W / V): corn flour 3%, soybean flour 3%, KH 2 PO 4 0.1%, MgSO 4 0.02%, V B1 0.001%, pH 6.5.

[0067] 3) Preparation method of bran extract: Take 40.8 grams of bran, add 1000mL tap water, boil for 1h, filter with double gauze, and dilute the filtrate to 1000mL to obtain the bran extract.

[0068] 4) The formula of t...

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Abstract

The invention discloses a method for preparing beta-carotene through fermentation of Blakeslea trispora. The method comprises the following steps of: after activating Blakeslea trispora ATCC14271 (+) and ATCC14272 (-), carrying out seed culture on the activated Blakeslea trispora; inoculating the Blakeslea trispora to a fermentation medium according to a certain inoculum concentration and inoculation ratio under an optimized fermentation process condition so as to ferment the Blakeslea trispora; and finally obtaining a mycelium with higher beta-carotene content. Through the adoption of the method disclosed by the invention, a natural high-quality beta-carotene product with low cost and high titer can be obtained.

Description

Technical field [0001] The invention belongs to the technical field of biological fermentation, and specifically relates to a method for preparing β-carotene by fermentation of B. trispora. Background technique [0002] β-carotene is not only pro-VA, it has the functions of preventing eye diseases and protecting eyesight, but also has the physiological functions of inhibiting cell mutation, anti-cancer, anti-cancer, anti-oxygen free radicals, enhancing body resistance, and maintaining normal human epithelial tissues. As well as a series of important functions such as promoting children's growth and development, beauty, etc., it is widely used as food nutrition fortifiers and additives in many countries in the world. [0003] The current preparation methods of β-carotene mainly include the following: (1) Extraction of natural β-carotene. In industrial production, β-carotene is often extracted from carrots, corn, sea buckthorn and other plants rich in β-carotene. (2) Chemical s...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12R1/645
Inventor 蔡俊王常高颜作文胡瑛林建国
Owner HUBEI UNIV OF TECH
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