Method for preparing beta-carotene through fermentation of Blakeslea trispora

A technology of B. boulardii and B. trispora is applied in the field of preparing beta-carotene, can solve the problems of high cost, low yield of beta-carotene, large amount of vegetable oil added, etc., and achieves low cost and solid content. Low content, cost and resource saving effect

Active Publication Date: 2012-10-31
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the yield of β-carotene prepared by this method is too low
In addition, Chinese patent CN1193048A discloses a method for fermenting and producing β-carotene by culturing filamentous fungi in an air-lift fermenter by using

Method used

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  • Method for preparing beta-carotene through fermentation of Blakeslea trispora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Preparation of β-carotene-rich strains by fermentation culture in shake flasks

[0024] 1. Strain:

[0025] B. trispora ATCC 14271 (+) and ATCC 14272 (-)

[0026] 2. Medium:

[0027] 1) Preparation and composition of inclined plane PDA medium: Take 200g of fresh unsprouted potatoes, peel them, cut into 3mm×3mm pieces, add 800-1000ml of water, boil for 30min, then filter with gauze, add sucrose to the filtrate ( Or glucose) 20g, 15-20g agar, replenish water to 1000ml after dissolution, natural pH, aliquot into test tubes (5ml per tube), sterilize at 121°C for 30min, and make a bevel.

[0028] 2) The seed medium formula is (W / V): corn flour 3%, soybean flour 3%, KH 2 PO 4 0.1%, MgSO 4 0.02%, V B1 0.001%, pH 6.5.

[0029] 3) Preparation method of bran extract: Take 40.8 grams of bran, add 1000mL tap water, boil for 1h, filter with double gauze, and dilute the filtrate to 1000mL to obtain the bran extract.

[0030] 4) The formula of the fermentation medium: take 10...

Embodiment 2

[0042] Example 2: Preparation of β-carotene-rich strains by fermentation culture in a 10L mechanically stirred fermentor

[0043] 1. Strain:

[0044] B. trispora ATCC 14271 (+) and ATCC 14272 (-)

[0045] 2. Medium:

[0046] 1) Preparation and composition of inclined plane PDA medium: Take 200g of fresh unsprouted potatoes, peel them, cut into 3mm×3mm pieces, add 800-1000ml of water, boil for 30min, then filter with gauze, add sucrose to the filtrate ( Or glucose) 20g, 15-20g agar, replenish water to 1000ml after dissolution, natural pH, aliquot into test tubes (5ml per tube), sterilize at 121°C for 30min, and make a bevel.

[0047] 2) The seed medium formula is (W / V): corn flour 3%, soybean flour 3%, KH 2 PO 4 0.1%, MgSO 4 0.02%, V B1 0.001%, pH 6.5.

[0048] 3) Preparation method of bran extract: Take 40.8 grams of bran, add 1000mL tap water, boil for 1h, filter with double gauze, and dilute the filtrate to 1000mL to obtain the bran extract.

[0049] 4) The formula of th...

Embodiment 3

[0061] Example 3: Preparation of β-carotene-rich strains by fermentation culture in a 100L mechanically stirred fermentor

[0062] 1. Strain:

[0063] B. trispora ATCC 14271 (+) and ATCC 14272 (-)

[0064] 2. Medium:

[0065] 1) Preparation and composition of inclined plane PDA medium: Take 200g of fresh unsprouted potatoes, peel them, cut into 3mm×3mm pieces, add 800-1000ml of water, boil for 30min, then filter with gauze, add sucrose to the filtrate ( Or glucose) 20g, 15-20g agar, replenish water to 1000ml after dissolution, natural pH, aliquot into test tubes (5ml per tube), sterilize at 121°C for 30min, and make a bevel.

[0066] 2) The seed medium formula is (W / V): corn flour 3%, soybean flour 3%, KH 2 PO 4 0.1%, MgSO 4 0.02%, V B1 0.001%, pH 6.5.

[0067] 3) Preparation method of bran extract: Take 40.8 grams of bran, add 1000mL tap water, boil for 1h, filter with double gauze, and dilute the filtrate to 1000mL to obtain the bran extract.

[0068] 4) The formula of t...

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Abstract

The invention discloses a method for preparing beta-carotene through fermentation of Blakeslea trispora. The method comprises the following steps of: after activating Blakeslea trispora ATCC14271 (+) and ATCC14272 (-), carrying out seed culture on the activated Blakeslea trispora; inoculating the Blakeslea trispora to a fermentation medium according to a certain inoculum concentration and inoculation ratio under an optimized fermentation process condition so as to ferment the Blakeslea trispora; and finally obtaining a mycelium with higher beta-carotene content. Through the adoption of the method disclosed by the invention, a natural high-quality beta-carotene product with low cost and high titer can be obtained.

Description

Technical field [0001] The invention belongs to the technical field of biological fermentation, and specifically relates to a method for preparing β-carotene by fermentation of B. trispora. Background technique [0002] β-carotene is not only pro-VA, it has the functions of preventing eye diseases and protecting eyesight, but also has the physiological functions of inhibiting cell mutation, anti-cancer, anti-cancer, anti-oxygen free radicals, enhancing body resistance, and maintaining normal human epithelial tissues. As well as a series of important functions such as promoting children's growth and development, beauty, etc., it is widely used as food nutrition fortifiers and additives in many countries in the world. [0003] The current preparation methods of β-carotene mainly include the following: (1) Extraction of natural β-carotene. In industrial production, β-carotene is often extracted from carrots, corn, sea buckthorn and other plants rich in β-carotene. (2) Chemical s...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12R1/645
Inventor 蔡俊王常高颜作文胡瑛林建国
Owner HUBEI UNIV OF TECH
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