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Method of preparing lycopene and lycopene product

A technology of lycopene and products is applied in the field of preparing lycopene and lycopene products, and can solve the problems of low lycopene yield and the like

Active Publication Date: 2017-12-15
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But, the lycopene yield obtained by the existing method of fermenting and producing lycopene with B. trispora is relatively low

Method used

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  • Method of preparing lycopene and lycopene product
  • Method of preparing lycopene and lycopene product
  • Method of preparing lycopene and lycopene product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] A kind of method for preparing lycopene provided by the present embodiment, it comprises the steps:

[0075] 1 slant culture

[0076] Take the spore suspensions of the positive bacteria and the negative bacteria of B. trispora, spread them on the PDA slant medium, and cultivate them in a constant temperature incubator at 25°C for 5-7 days;

[0077] Among them, the positive bacterium of B. trispora is B. trispora BT7251 (+), and the preservation number is CCTCCM2014378; the negative bacterium of B. trispora is B. trispora BT7603 (-), and the storage number is CCTCCM2014379.

[0078] Among them, the PDA slant medium includes: glucose 20g / L, agar powder 25g / L, peeled potatoes 200g / L; its preparation method can be referred to as follows: cut potatoes into 1cm cubes, add deionized water to boil for 30 minutes, cool and use Filter with four layers of gauze, take the filtered supernatant and add glucose and agar powder.

[0079] 2 seed culture

[0080] Use an inoculation s...

Embodiment 2

[0104] The method for preparing lycopene provided in this example is basically the same as that in Example 1, except that the biological enzyme added during the fermentation and cultivation in Step 3 is pectinase (50000 U / g). The test results are shown in Table 1.

Embodiment 3

[0106] The method for preparing lycopene provided in this example is basically the same as that in Example 1, except that the biological enzyme added during the fermentation process in Step 3 is neutral protease (100,000 U / g). The test results are shown in Table 1.

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Abstract

The invention discloses a method of preparing lycopene and a lycopene product. The method of preparing lycopene, disclosed by the invention, comprises the step of adding a bio-enzyme and a blocking agent into fermenting liquor containing positive blakeslea trispora and positive blakeslea trispora in a fermenting culture process for enzymolysis, wherein the bio-enzyme is selected from one or combination of more of pectinase, cellulase, neutral protease and lipase. By means of action of the bio-enzyme, enzymolysis is performed on adhered substances such as pectic substance, proteins and lipid among hyphae, so that the aims of reducing the viscosity of the fermenting liquor, improving the distributing degree of hyphae, and increasing dissolved oxygen in the fermenting liquor effectively so as to improve the output of the lycopene and improve the product quality are achieved.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a method for preparing lycopene and a lycopene product. Background technique [0002] Lycopene is a fat-soluble carotenoid that can efficiently quench singlet oxygen and scavenge free radicals. Its antioxidant activity is the strongest among carotenoids. It has a certain effect on delaying aging and so on, and it is a functional natural pigment with great development value. [0003] The production of lycopene raw materials mainly includes plant extraction, chemical synthesis and microbial fermentation. The main raw material required by the plant extraction method is tomato. Tomato planting is limited by climate, place of origin, transportation and other conditions, and the extraction process is cumbersome and lengthy, and the cost is expensive, which cannot meet the needs of industrial production. Although the cost of chemical synthesis is low, its impact on the...

Claims

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Application Information

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IPC IPC(8): C12P5/02A61K31/01A61P9/00A61P37/02A61P3/02C12R1/645
CPCC12P5/026
Inventor 李翔宇汪志明余超杨艳红陆姝欢
Owner CABIO BIOTECH WUHAN CO LTD
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