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Method for improving yield of lycopene produced by utilizing Blakeslea trispora

A technology based on brucella and lycopene, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of difficult to buy, less than lycopene yield, unstable intermediate metabolites, etc. problem, to achieve the effect of simple method, increase yield and reduce production cost

Active Publication Date: 2011-11-23
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Intermediate metabolites such as GPP, IPP, DMAPP, and GGPP are unstable and difficult to buy in the market. They cannot be directly used for addition, and the purpose of increasing lycopene production will not be achieved.

Method used

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  • Method for improving yield of lycopene produced by utilizing Blakeslea trispora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) plate culture

[0030] Take 20g of peeled potatoes, add 200mL of deionized water to boil, keep boiling for 20 minutes, then cool, filter out the potatoes, add 2g of glucose and 2g of agar to the clear liquid, dissolve them, put them into a 500mL Erlenmeyer flask, and sterilize at 115°C for 30min , pour it into a plate while it is hot, and cool down to prepare a solid PDA medium. Take the spore suspension of Bradleyella trispora (+) (-) strain, and spread it on the plate containing PDA medium with a glass rod. After the coated plates were cultured in an incubator at 28°C for 40h, they were left at room temperature for 24h.

[0031] (2) Pretreatment of isopentenol

[0032] The prenol was diluted, and 400 uL of prenol (97% purity) was dissolved in 10 ml of 75% ethanol to prepare a solution with a concentration of 32 mg / mL.

[0033] (3) Preparation of seed medium

[0034]Add 20g of starch, 25g of corn steep liquor, and 12.5g of glucose into a 1L beaker, set the volu...

Embodiment 2

[0046] (1) plate culture

[0047] Take 20g of peeled potatoes, add 200mL of deionized water to boil, keep boiling for 20 minutes, then cool, filter out the potatoes, add 2g of glucose and 2g of agar to the clear liquid, dissolve them, put them into a 500mL Erlenmeyer flask, and sterilize at 115°C for 30min , pour it into a plate while it is hot, and cool down to prepare a solid PDA medium. Take the spore suspension of B. trispora (+) (-) strain. After the coated plates were cultured in an incubator at 28°C for 40h, they were left at room temperature for 24h.

[0048] (2) Pretreatment of geraniol

[0049] Geraniol was dissolved and diluted, and 40 uL of geraniol (purity 98%) was dissolved in 1 ml of 75% ethanol to prepare a solution with a concentration of 32 mg / mL.

[0050] (3) Preparation of seed medium

[0051] Add 20g of starch, 25g of corn steep liquor, and 12.5g of glucose into a 1L beaker, set the volume to 500mL with tap water, put it on a water bath, gelatinize at ...

Embodiment 3

[0063] (1) plate culture

[0064] Take 20g of peeled potatoes, add 200mL of deionized water to boil, keep boiling for 20 minutes, then cool, filter out the potatoes, add 2g of glucose and 2g of agar to the clear liquid, dissolve them, put them into a 500mL Erlenmeyer flask, and sterilize at 115°C for 30min , pour it into a plate while it is hot, and cool down to prepare a solid PDA medium. Take the spore suspension of the B. trispora (+) (-) strain and spread it on a plate containing PDA medium with a glass rod. After the coated plates were cultured in an incubator at 28°C for 40h, they were left at room temperature for 24h.

[0065] (2) Pretreatment of 3-methyl-3-buten-1-ol

[0066] 3-methyl-3-buten-1-ol was diluted, and 40 uL of prenol (purity 97%) was dissolved in 1 ml of 75% ethanol to prepare a solution with a concentration of 30 mg / mL.

[0067] (3) Preparation of seed medium

[0068] Add 20g of starch, 25g of corn steep liquor, and 12.5g of glucose into a 1L beaker, ...

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Abstract

The invention provides a method for improving yield of lycopene produced by utilizing Blakeslea trispora, and the method is characterized by comprising the following steps: (1) carrying out plate culture on Blakeslea trispora (+)(-) strain spore suspensions and dissolving one of prenol, 3-methyl-3-butene-1-ol, geraniol and mevalonolactone in ethanol to obtain a solution; (2) preparing a seed culture medium; (3) separately adding plate-cultured Blakeslea trispora (+)(-) strains to a triangular flask containing the seed culture medium; and (4) preparing a fermentation medium, mixing 1 bottle ofcultured (+) strain seed liquid and 4 bottles of cultured (-) strain seed liquid, adding the fermentation culture medium to the seed liquid for starting fermentation, then adding the solution of one of prenol, 3-methyl-3-butene-1-ol, geraniol and mevalonolactone 0-36 hours after the start of the fermentation and then carrying out constant-temperature culture. The method provided by the invention is simple and easy to operate and control, substantially improves the yield of lycopene and lowers production cost.

Description

technical field [0001] The present invention relates to a method for increasing the yield of lycopene produced by B. trispora, which belongs to the research field of improving microbial liquid fermentation to produce secondary metabolites by adding a series of precursor substances, and particularly relates to a method for producing secondary metabolites by adding a series of precursor substances A method of increasing the biosynthetic amount of lycopene by adding prenol, geraniol, 3-methyl-3-buten-1-ol and mevalonolactone. Background technique [0002] Lycopene is a fat-soluble unsaturated hydrocarbon, belonging to isoprene compounds, with 11 conjugated double bonds and 2 non-conjugated double bonds, molecular formula C40H56, molecular weight 536.85Da, molecular structure as follows: [0003] [0004] Lycopene is an important fat-soluble carotenoid, which has important physiological functions such as strong anti-oxidation, scavenging free radicals, anti-cancer and tumor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12R1/645
Inventor 袁其朋师艳秋辛秀兰
Owner BEIJING UNIV OF CHEM TECH
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