104 results about "Isopentenol" patented technology

The invention discloses production of terpenoids and a strain, and belongs to the technical field of bioengineering. The serratia marcescens engineering bacteria for producing the semi-terpenoids or monoterpenoids are constructed, and can be used for producing linalool, isoprene, isopentenol, 1, 3, 4-triterpenes and 1, 3, 4-triterpenes. The composition is prepared from 1, 8-cineole, alpha-pinene, pinene, gamma-terpinene, geraniol, (+)-limonene, (-)-limonene, myrcene, beta-ocimene, sabinene, (-)-alpha-bisabolol, (-)-alpha-bisabolol, longifolene, wallenenes, beta-elemene, farnesene, patchouli alcohol, acyclic terpene and alpha-santalene. In a 30L fermentation tank, the yield of linalool produced by the serratia engineering bacteria is 40.72 g.L <-1 >.

The invention discloses a high-yield delta-tocotrienol genetically engineered bacterium and application thereof, and belongs to the technical field of gene recombination. The invention discloses a genetically engineered bacterium for high yield of delta-tocotrienols, which is characterized in that yarrowia lipolytica ATCC MYA-2613 is used as an original strain, and a ku70 gene is knocked out; the gene is used for expressing and coding 4-hydroxyphenylpyruvate dioxygenase, the gene is used for coding uromelanic acid phytate transferase and the gene is used for coding tocopherol cyclase; intensifying a shikimic acid pathway; a mevalonic acid pathway is strengthened; an isopentenol path is introduced; carrying out protein fusion on the HPD and the HPT; truncating a signal peptide of a gene encoding a tocopherol cyclase and back-filling a leucine defect. The genetically engineered bacterium constructed by the invention has industrial stability, can be amplified by more than 10 times in a 2L fermentation tank level compared with a shake flask level, can produce 616.45 mg / L of delta-tocotrienol after being cultured for 240 hours, and has industrial application prospects.

The invention provides a method for preparing isopentenol from 3-methyl-1-butene, which comprises the following steps: oxidizing 3-methyl-1-butene to obtain a 2-isopropyl ethylene oxide intermediate, and then isomerizing the intermediate to obtain the isopentenol. The synthesis route is novel, the cheap refined C5 byproduct 3-methyl-1-butene is used as a starting raw material, the isopentenol is simply and efficiently prepared through two-step reaction of epoxidation, isomerization and the like, and an economical and feasible route is provided for preparation of the product; and secondly, in the isomerization reaction in the second step, lithium phosphate treated by sodium alcoholate or potassium alcoholate is used as a catalyst, so that the isomerization reaction of the epoxy intermediate is efficiently realized, and the isopentenol is conveniently and quickly prepared. # imgabs0 #

The invention discloses Klebsiella and application thereof, and belongs to the technical field of microorganisms. According to the invention, firstly, a strain LLMJ-DNXJ-01 with high yield of tannase is screened from wine-making yeast, the identification result shows that the strain belongs to klebsiella, then the tannase activity of the strain is investigated, and the result shows that the enzyme activity reaches 8.8 + / -0.02 U / mL. In addition, the invention also investigates the application of the strain in degrading sorghum tannin, the sorghum tannin degradation rate reaches 34.95%, and isoamyl alcohol, isopentenol, caryophyllene, phenethyl alcohol, 4-vinyl guaiacol and other volatile aroma substances with Daqu characteristics can be generated through fermentation. Therefore, by integrating the tannase production capability of the strain LLMJ-DNXJ-01 and the sorghum tannin degradation and fermentation aroma production capability, the strain has relatively good development potential in the aspects of manufacturing of reinforced yeast for making hard liquor and quality improvement and aroma enhancement of white spirit.

The invention discloses a staged delayed coagulation and viscosity reduction type iron aluminate cement water reducing agent and a preparation method thereof. The iron aluminate cement water reducing agent is obtained by copolymerizing prepared isopentenol polyoxyethylene ether borate, hydroxyethyl methylacrylate glucolactone and methacrylic acid. The boric acid ester is rapidly hydrolyzed in the initial stage of hydration (9 < pH < 12) to obtain boric acid, and first-stage delayed coagulation is performed on the aluminoferrite cement. When delayed coagulation fails, the cement continues to be hydrated to reach the middle stage of hydration (pH > 12), glucolactone is hydrolyzed to generate gluconate for further delayed coagulation of the cement, meanwhile, hydroxyethyl methylacrylate is hydrolyzed to release more adsorption sites, and hydration products are further dispersed to reduce the viscosity of the system. And stage release enables the aluminoferrite cement to be hydrated stage by stage, so that the problem of poor bleeding cohesiveness caused by non-hydration of the cement after one-time doping of the retarder is solved, the problem of slurry tackifying caused by large hydration of the cement in the later stage is also avoided, and engineering application of the aluminoferrite cement is further promoted.

The invention provides a method for improving the selectivity of a product 3-methoxy-3-methyl-1-butanol (MMB). According to the method, the content of the isopentene methyl ether in the raw material isoprenol is controlled to be 0.005-0.25 wt%, so that the selectivity of the MMB can be effectively improved, the generation of key byproducts of isoprenol methyl ether and isoprenol methyl ether is reduced, and the purity of the MMB is improved.

The invention belongs to the technical field of building materials, and particularly relates to a silane modified starch-based polycarboxylate superplasticizer and a preparation method thereof. The water reducing agent is prepared by taking renewable sulfonated starch as a raw material, performing graft modification through vinyltriethoxysilane (A151), introducing active double bonds into starch molecules, and then copolymerizing with monomers such as isopentenol polyoxyethylene ether (TPEG) and acrylic acid. Compared with the prior art, the mixing amount of the vinyltriethoxysilane (A151) is only 1%-5% of the mass fraction of the acrylic acid, the mixing amount is low, and the cost is saved; the water reducing and dispersing performance of the starch-based polycarboxylic acid water reducing agent is improved, and a certain viscosity reducing effect is achieved.

The invention discloses a method for producing terpenoids, and belongs to the technical field of bioengineering. The serratia marcescens engineering bacteria for producing the semi-terpenoids or monoterpenoids are constructed, and can be used for producing isoprene, isopentenol, 1, 3, 4-triterpenes, 1, 3, 4-triterpenes and the like. The composition is prepared from 1, 8-cineole, alpha-pinene, pinene, gamma-terpinene, geraniol, (+)-limonene, (-)-limonene, myrcene, beta-ocimene, sabinene, (-)-alpha-bisabolol, (-)-alpha-bisabolol, longifolene, wallenenes, beta-elemene, farnesene, patchouli alcohol, acyclic terpene and alpha-santalene. In a 30L fermentation tank, the yield of linalool produced by the serratia engineering bacteria is 13.49 g.L <-1 >.

The invention discloses a viscosity reduction type lithium iron phosphate precursor slurry dispersing agent as well as a preparation method and application thereof, and belongs to the technical field of battery materials. The dispersing agent is prepared by taking isopentenol polyoxyethylene ether, unsaturated carboxylic acid or salt thereof, unsaturated substituted benzene ring or salt thereof and substituted acrylamide as reaction monomers through free radical polymerization under the action of an initiator and a chain transfer agent. On the premise that the solid content of the lithium iron phosphate precursor slurry is not reduced or improved, the viscosity of the slurry is reduced, the dispersity and the aging stability of the slurry are improved, the slurry is prevented from settling and flocculating before spray drying or in the spray drying process, the production efficiency is improved, the energy consumption in the production process is reduced, and the production cost is further reduced.

The invention relates to the technical field of building materials, in particular to a method for preparing high-strength flow-state solidified soil by utilizing industrial solid waste, which comprises the following steps: taking industrial solid waste mixed materials such as steel slag, mineral powder, ardealite, carbide slag and the like as a curing agent, taking redundant soil as raw material soil, taking modified coconut fiber as a reinforcement material, and introducing a composite tackifier to prepare the high-strength flow-state solidified soil. The preparation method comprises the following steps: firstly, epoxidizing coconut shell fibers, and then performing one-pot reaction on the epoxidized coconut shell fibers, branched polyethyleneimine and triglycidyl p-aminophenol to obtain amino-terminated hyperbranched coconut shell fibers serving as modified coconut shell fibers; vinyl-terminated epoxy organic silicon, isopentenol polyoxyethylene ether, N, N-dimethylacrylamide and acrylic acid are used as raw materials to synthesize the composite tackifier, so that the durability of the fluid-state solidified soil is greatly improved.

The invention provides a hydrogenation catalyst and a preparation method thereof, and a method and a system for continuously producing isopentenol. The hydrogenation catalyst comprises a carrier, an active component and an auxiliary component, wherein the active component and the auxiliary component are loaded on the carrier; the method for continuously producing the isopentenol comprises the following steps: feeding a raw material flow containing hydrogen and 3-methyl-3-butene-1-ol into a reactor from an inlet in the bottom of the reactor, and obtaining a reacted material flow at an outlet in the top of the reactor; carrying out gas-liquid separation to obtain circulating hydrogen and a liquid-phase material flow; one part of the circulating hydrogen is circulated to the reactor, and the rest part is moved out of the reaction zone; a portion of the liquid phase stream is recycled to the reactor, and the remaining portion is collected as a product. When the catalyst is used for continuously producing the isopentenol, the conversion rate of the 3-methyl-3-butene-1-ol is 30%, the selectivity of the isopentenol can be up to 96%, and the catalyst can continuously and stably run for more than 1200 hours.

The invention relates to an isopentenol polyoxyethylene ether defoaming agent and a preparation method thereof, the defoaming agent is mainly prepared from a cross-linked acrylic copolymer emulsion, an alkali swelling thickener, a modified organic silicon defoaming active matter, a pH regulator (NaOH / KOH), an isothiazolinone bactericide, deionized water and the like in proportion, and the defoaming agent has excellent defoaming performance and stability. A specific auxiliary agent is added. The foam problem of an isopentenol polyoxyethylene ether product in downstream application is solved, and the defoaming agent is mainly applied to defoaming of isopentenol polyoxyethylene ether.

The invention discloses a processing technology for preparing isopentenol through isomerization of methyl butenol, and relates to the technical field of organic chemical preparation. The method at least comprises the following steps: S1, stirring a reactor, adding methyl butenol with a certain water content, a catalyst and a cocatalyst into the stirring reactor, and then heating under a stirring condition; s2, the dosage of the main catalyst and the dosage of the cocatalyst are determined, the wt% of the main catalyst in a reaction system is 0.5%-2.0%, and the wt% of the cocatalyst is 0.5%-2.0% of that of the main catalyst. According to the present invention, the water-containing methyl butenol is adopted as the raw material, the ammonium metavanadate is adopted as the catalyst, and the small amount of the nitrogen-containing compound is adopted as the ligand to perform the isomerization reaction to obtain the isopentenol 321, such that the new process is provided based on the deficiency of the early stage production process, the problem of the preparation of the isopentenol 321 through the isomerization of the methyl butenol is systematically solved, the continuous and stable production of the production device is ensured, and the production cost is reduced. The product yield is increased, the energy consumption is greatly reduced, and the wastewater discharge amount is greatly reduced.

The present application relates to the field of fluidized solidified soil, and discloses a kind of high-flow self-compacting fluidized solidified soil and its preparation process, including dry base engineering slag soil, mixing water, sodium hexametaphosphate, L (+) -tartaric acid, composite cementitious material, warm rubber dry powder and isoamyl alcohol polyoxyethylene ether polycarboxylic acid water reducing agent are made.The composite cementitious material is premixed with warm rubber dry powder;Slag soil is mixed with water and sodium hexametaphosphate to disperse internal mud group;Under continuous stirring, add tartaric acid aqueous solution, control the drop amount by using real-time monitoring of liquid phase pH value and closed-loop mechanism of tartaric acid consumption ratio, neutralize alkaline and inhibit early hydration heat release of cementitious material;Finally, add powder and water reducing agent for secondary stirring, and discharge and pour when liquid phase pH value jumps.The present application overcomes the hydration out of control caused by the fluctuation of different slag components through chemical synergy and dynamic feeding feedback of multiple components, avoids settlement stratification and bleeding, and ensures the stable development of high-flow state and later mechanical strength of slurry.

The application belongs to the field of microbial metabolic engineering, and discloses a Saccharomyces cerevisiae engineering strain with enhanced isopentenol tolerance and conversion capacity, and a construction method and application thereof. The Saccharomyces cerevisiae engineering strain is obtained by modifying an isopentenol utilization pathway-dependent Saccharomyces cerevisiae strain, and the energy metabolism of the Saccharomyces cerevisiae strain is enhanced to synthesize a large amount of ATP for the isopentenol utilization pathway; and the antioxidant stress resistance of the Saccharomyces cerevisiae strain is enhanced to relieve the oxidative damage caused by the isopentenol substrate. The Saccharomyces cerevisiae engineering strain is the strain with the highest efficiency in converting isopentenol into terpenes.

The invention discloses a saccharomyces cerevisiae genetically engineered bacterium for efficiently synthesizing mogroside V based on multi-compartment multi-metabolic pathways and a construction method of the saccharomyces cerevisiae genetically engineered bacterium. The preparation method comprises the following steps: anchoring an MVA pathway and a squalene synthesis pathway on the surface of a peroxisome membrane, and enabling mogrol synthetase and epoxy squalene cyclization enzyme ERG1 to form a linear polywheat complex through polypeptide interaction; then, through overexpression of MVA pathway key enzyme and introduction of an isopentenol utilization pathway, supply of precursor isopentenyl pyrophosphate and dimethyl allyl pyrophosphate in cytoplasm is increased, and squalene epoxidase ERG1 and mogrol synthetase are anchored in transmembrane ABC efflux protein PDR11 through polypeptide interaction; then anchoring the mogrol synthetase in the lipid droplet subcells; finally, the glucosyltransferase is displayed on the surface of the yeast cell wall through an alpha-lectin system, so that extracellular mogrol is converted into MG-V. The engineering bacterium improves the yield of mogroside V synthesized from the beginning in microorganisms, and has great industrial application value.

The present invention relates to a method for the preparation of 3-methyl-3-butene-1-ol (isoprenol), comprising the steps of preparing isobutylene (also: isobutene, 2-methylpropene) by contacting 3-methylbutan-1-ol (isoamyl alcohol) to a catalyst comprising at least one catalytically active metal and reacting at least one formaldehyde source and the isobutylene obtained in step a-i) to obtain isoprenol. Furthermore, the present invention refers to processes for the preparation of 3-methyl-2-butene-1-ol (prenol), of 3-methyl-3-butenal (isoprenal), of 3-methyl-2-butenal (prenal), and of 3,7-dimethy l-octa-2, 6-dienal (citral) and further downstream products comprising the use of isoprenol of the present invention and the resulting products and product streams.

The application relates to the field of building materials and discloses a sound and heat insulation light building material and a preparation method thereof. The light building material comprises cement, fly ash, river sand, quartz sand, a foaming agent, a modified composite filler-based water reducing agent and water. The foaming agent is made of sodium dodecyl sulfate, hydroxypropyl methyl cellulose, modified nano-aluminum oxide and nano-silicon dioxide. The modified nano-aluminum oxide is prepared by the following steps: reacting oleic acid with nano-aluminum oxide, emulsifying through a Pickering emulsion method, and selectively dissociating through a saponification reaction induced by sodium hydroxide. The modified composite filler-based water reducing agent is prepared by the following steps: in-situ esterification modification of a composite filler made by grafting isoprenyl alcohol polyoxyethylene ether on graphene oxide and carbon fiber, and then free radical copolymerization with acrylic acid. The light building material prepared by the application has high compressive strength and flexural strength, small water absorption and good heat insulation performance.

This invention belongs to the field of synthetic biology and microbial engineering, and discloses a chemical-biological method for preparing the isopentenyl bibenzyl compound 2-isopentenyl-3,3',4',5-tetrahydroxybibenzyl (NPB-1575) which has anti-ischemic stroke activity. The specific disclosed method includes: (1) chemically synthesizing the substrate dihydropiperidine; (2) constructing an engineered Escherichia coli strain based on the isopentenyl utilization pathway, co-expressing hydroxyethylthiazol kinase, isopentenyl phosphokinase, isopentenyl pyrophosphate isomerase and specific isopentenyl transferase, and efficiently synthesizing the isopentenyl donor; (3) using this strain to catalyze dihydropiperidine in whole cells, the yield in a 200L fermenter can reach 2.54g / L, which is nearly 40 times higher than the existing method; (4) obtaining NPB-1575 with a purity >98.5% by macroporous adsorption resin and normal / reversed phase chromatography and recrystallization. This method uses readily available raw materials, involves simple steps, is environmentally friendly, and yields high purity, laying the foundation for the development of NPB-1575 as a new drug for ischemic stroke and showing significant application potential.

The application discloses a synthesis method of lily pyran. The synthesis method uses 3-methyl-3-buten-1-ol (isopentenol) and isovaleraldehyde as raw materials, and performs a reaction in the presence of a catalyst to generate lily pyran. The catalyst is a supported catalyst, and comprises a carrier and a coating layer supported on the carrier. The coating layer comprises a Nafion polymer and an inorganic oxide, and the carrier is in a foamed state. Preferably, the carrier is selected from a combination of one or more of silicon carbide foam, aluminum foam, nickel foam and iron foam. A solution of the Nafion polymer, water and a precursor of the inorganic oxide are mixed to form a sol under stirring, and then the sol is coated on the carrier and dried to obtain the catalyst. The catalyst is used for the synthesis of lily pyran, and has the advantages of high raw material conversion rate, high product selectivity, less three-waste emissions and excellent catalyst stability.

The invention discloses a method for producing a composite water reducing agent capable of realizing resource utilization, which specifically comprises the following steps: mixing isopentenol polyoxyethylene ether, acrylic acid, hydroxyethyl acrylate and water to obtain a mixed solution A, mixing acrylamide, dimethyl diallyl ammonium chloride, sodium methallyl sulfonate and water to obtain a mixed solution B, and mixing the mixed solution B with water to obtain the composite water reducing agent. The mixed solution A and the mixed solution B are mixed and heated, an initiator and a chain transfer agent are added for a reaction, and a modified water reducer solution is prepared; porous graphene is added into the modified water reducing agent solution, ball milling and filtering are conducted, sediment is washed with deionized water and then dried, the composite water reducing agent is prepared, and waste liquid generated in the process is reused for producing the modified water reducing agent solution. The prepared composite water reducing agent is good in water reducing effect, the mechanical property of a cement-based material can be effectively improved, waste liquid generated in production is recycled and reused, the preparation cost of the water reducing agent is reduced, and the situation that the environment is polluted by discharged waste liquid is avoided.

The present application discloses a method for preparing a sodium carboxybenzenesulfonate modified polycarboxylic acid water reducer, which relates to the technical field of building materials, including S100. preparing a mixed solution A containing PEGMA-SSBS monomer; S200. preparing a mixed solution B; S300. synthesizing a sodium carboxybenzenesulfonate modified polycarboxylic acid water reducer PCE-B. The present application introduces sodium 3-carboxybenzenesulfonate containing -SO3- and -COOH groups and polymethacrylate to synthesize a modified monomer by free radical polymerization, and then synthesizes a modified polycarboxylic acid water reducer having a diester bond and sodium sulfonate with isopentanol polyoxyethylene ether and acrylic acid, so that the synthesized PCE-B has a promoting effect on the initial cement paste fluidity, and at the same time, the addition of the modified monomer PEGMA-SSBS significantly improves the adsorption capacity of PCE-B to cement particles.

The application discloses a process for preparing 3-methyl-2-buten-1-al diisopentyl acetal by continuous reaction rectification. The process comprises the following steps: reacting and rectifying isopentenyl alcohol and isopentenyl aldehyde in a reaction rectification tower to prepare 3-methyl-2-buten-1-al diisopentyl acetal, characterized in that the content of 2-methyl-3-buten-2-al in the liquid phase of the third to fifth trays of the reaction rectification tower from bottom to top is less than or equal to 1 wt%. By the continuous reaction rectification process, the device can be stably operated for more than 7200 hours, and the economic benefit of the enterprise can be remarkably improved.

The invention belongs to the technical field of pipe pieces, and particularly relates to a composite prefabricated concrete pipe piece and a preparation method thereof. The composite prefabricated concrete duct piece comprises a duct piece body and a reinforcement cage arranged in the duct piece body, the duct piece body is made of a concrete material, and the concrete material comprises cement, a mineral admixture, coarse aggregate, fine aggregate, an early-strength polycarboxylate superplasticizer, a functional admixture and water. The early strength type polycarboxylate superplasticizer is prepared by taking the isopentenol polyoxyethylene ether, the triallyl phosphate, the L-vinylglycine and the acrylic acid as monomers, so that the early strength of concrete is improved; modified polypropylene fiber, lithium silicate and methyl cellulose are adopted to jointly form a functional admixture, hydroxyethyl methylacrylate is grafted to the surface of the polypropylene fiber, and the anti-cracking performance, the anti-seepage performance and the mechanical performance of the concrete are improved.

The invention discloses a cotton fabric crease-resistant finishing method for constructing a simulated semi-interpenetrating polymerization network based on in-situ polymerization, isopentenol polyoxyethylene ether macromonomer copolymerization is introduced in the method, and on the basis of in-situ polymerization, maleic anhydride and acrylic acid are used for jointly realizing the crease-resistant finishing effect of polycarboxylic acid; by means of the structural characteristics of the isopentenol polyoxyethylene ether macromonomer, an imitated semi-interpenetrating polymerization network structure is constructed in the cotton fabric, and anti-wrinkle finishing of the cotton fabric is achieved. Under the optimal process conditions, the optimal anti-wrinkle finishing effect is obtained, the wrinkle recovery angle WRA is 252.2 degrees, and the whiteness is 73.6. The obtained wrinkle recovery angle WAR of the original cloth sample is 141.5 degrees, and the whiteness is 80.7. The wrinkle recovery angle is obviously increased, the anti-wrinkle effect is obvious, the whiteness maintaining effect is good, and yellowing is small. The method is easy to operate, raw materials are easy to obtain, enterprise cost can be saved, and environmental protection and industrial production are facilitated.

The invention belongs to the technical field of enzyme engineering, and discloses a high-activity isopentenyl phosphokinase mutant and application thereof. The high-activity isopentenyl phosphokinase mutant is AtIPKH274P, and the amino acid at the 274 site of wild type AtIPK is mutated from histidine H to proline P. The invention further discloses a preparation method of the high-activity isopentenyl phosphokinase mutant. According to the present invention, the AtIPKH274P, the SmDAGKS47A and the L124A can form the efficient isopentenol utilization pathway so as to effectively convert the prenol into the DMAPP; an engineering strain with improved isopentenyl modification capability can be constructed by utilizing the approach, and the strain has more advantages compared with a traditional engineering strain based on a mevalonic acid approach.

The application provides a method for synthesizing 3-methyl-2-buten-1-ol, and belongs to the technical field of isopentenol synthesis. The method comprises the following steps: introducing 3-methyl-3-buten-1-ol and an atmosphere containing hydrogen into a fixed bed reactor, and performing a hydrogenation isomerization reaction under the action of a Pd / Al2O3 catalyst to generate 3-methyl-2-buten-1-ol; wherein the content of formaldehyde in the 3-methyl-3-buten-1-ol is 0.03-0.15%. The application adopts a fixed bed reactor to perform continuous production, adopts a Pd / Al2O3 catalyst with low palladium content as the catalyst, the preparation method is simple, the price is relatively low, and the content of formaldehyde in the 3-methyl-3-buten-1-ol is controlled to inhibit a side reaction, so that the reaction conversion rate and the product selectivity are high.

The invention belongs to the field of microbial metabolic engineering, and discloses a saccharomyces cerevisiae engineering strain with enhanced isopentenol tolerance and transformation capacity as well as a construction method and application thereof. According to the invention, an isopentenol utilization pathway dependent type saccharomyces cerevisiae strain is modified, and energy metabolism of the isopentenol utilization pathway dependent type saccharomyces cerevisiae strain is enhanced, so that a large amount of ATP is synthesized for an isopentenol utilization pathway; the anti-oxidative stress capability is enhanced, so that oxidative damage caused by an isopentenol substrate is relieved. The saccharomyces cerevisiae engineering bacterium is a bacterial strain with the highest efficiency of converting the isopentenol into the terpenoids at present.

The invention discloses a multi-gene tandem recombinant strain as well as a construction method and application thereof. The invention also discloses a method for synthesizing lycopene based on an isopentenol utilization approach. The strain comprises a recombinant strain of seven key genes, namely mtipk, thim, ispA, idi, crtE, crttheB and crtI, for synthesizing lycopene in an IUP (Inhibitory Urinary Protein) way. According to the invention, the isopentenol is introduced into the escherichia coli in a way to synthesize the lycopene; according to the approach, only two-step phosphorylation reaction is used for replacing the original 2-C-methyl-D-erythritol-4-phosphoric acid approach and mevalonic acid approach which need more than seven steps to synthesize precusor substances pentadienyl pyrophosphoric acid and dimethyl allyl pyrophosphate ester of lycopene, so that the metabolic pathway is greatly shortened, and competition with central carbon metabolism in cells is avoided; the toxicity caused by accumulation of intermediate products in the metabolic process is reduced, and lycopene can be efficiently synthesized by using a cheap substrate.

The application provides an acid anhydride low-sensitivity polycarboxylic acid water reducing agent and a preparation method and application thereof, an intermediate is prepared by alcoholysis of maleic anhydride and ring-opening polymerization of maleic acid monoester and ethylene oxide, the carboxyl in the maleic acid ester is esterified by ethylene oxide, a part of carboxylic acid is protected, crosslinking of the main chain is prevented, and then the polymer quality is improved to prevent the generation of later-stage precipitation; and the esterification protects the carboxyl, further improves the slump flow retention performance, makes the slump flow retention agent content lower in the application process of the water reducing agent, and the effect is more obvious. Compared with the prior art, the isopentenyl alcohol polyoxyethylene ether is copolymerized with maleic anhydride and a functional monomer of the maleic anhydride to synthesize a high-conversion-rate low-sensitivity viscosity-reducing mother liquor, the solid content of the base liquid and the catalyst dosage are adjusted, so that the application can occur the polymerization reaction at normal temperature, and the energy consumption is low at normal temperature. The intermediate after functionalization of the maleic anhydride is used to participate in the free radical polymerization reaction, and the monomer conversion rate of the reaction product is generally higher than 80%.