Lycopene high-yielding strain and application thereof

A technology of lycopene and high-yielding strains, applied in fungi, microorganisms, biochemical equipment and methods, etc., can solve the problems of cumbersome operation and increase the risk of bacterial infection, and achieve simple operation, great social significance and economic value, cost reduction effect

Active Publication Date: 2014-03-26
CHANGZHOU BY YANG BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since positive and negative bacteria need to be mixed and fermented in proportion, the operation in this process is cumbersome and increases the risk of bacterial contamination

Method used

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  • Lycopene high-yielding strain and application thereof
  • Lycopene high-yielding strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: This example illustrates the method of mutagenizing the starting strain of B. trispora.

[0035] Taking the B. trispora VR-5(-) preserved in the laboratory as the starting strain, the mutagenesis is carried out, and the specific steps are as follows:

[0036] (a) Preparation of monospore suspension: the spores of the starting strain of B. trispora were prepared into a spore suspension, and there was no overlapping of cells under microscope inspection.

[0037] (b), in order to obtain the lycopene high-yield bacterial strain, after comparing multiple mutagenesis methods, select nitrosoguanidine (MNNG) to mutagenize the three spora boulardii ( blakeslea trispora ) strain method. from B. trispora in liquid medium ( blakeslea trispora ) culture to obtain the cell suspension to be mutagenized. At 20°C and 100rpm, the 10 8 Cells / ml in 0.1M sodium citrate buffer solution of 250ug / ml MNNG solution, pH 5.0 medium temperature bath for 60-120min, then inoculate the ...

Embodiment 2

[0043] Example 2: This example illustrates the passage stability of mutant strains.

[0044] Bacteria trispora boulardii of the present invention blakeslea trispora The main morphological and biological characteristics of V-6515 are as follows:

[0045] The colony is light yellow on the PDA medium and grows under aerobic conditions. The colony is large and round, with thick mycelium, neat edges, and uniform texture. The suitable growth temperature is 27.5-28.5°C. The suitable pH for growth is 7.0-7.2.

[0046] Detection of Mutant B. trispora in Fermentation Medium Using Cassava Flour as Carbon Source blakeslea trispora The subculture stability of V-6515, the strain subculture fermentation test results are shown in Table 1:

[0047] Table 1 High-yielding mutant strains of B. trispora blakeslea trispora V-6515 stability test

[0048]

[0049] It can be seen from the experimental results that after 5 passages, the mutant strain B. trispora blakeslea trispora The lyco...

Embodiment 3

[0050] Embodiment 3: This embodiment illustrates high-yield bacterial strain B. trispora B. trispora blakeslea trispora V-6515 fermentation process for producing lycopene.

[0051] The medium formula used in this embodiment:

[0052] The solid medium is: cassava flour 3.0%, malt extract powder 1.0%, agar powder 1.5%, pH 7.0-7.2, and distilled water.

[0053] The seed medium is: cassava flour 4.0%, soybean cake flour 2.0%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.05%, pH 7.0-7.2, tap water configuration.

[0054] The fermentation medium is: cassava flour 2.0%, soybean cake flour 4.0%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.05%, corn oil 3.0%, vitamin B 1 0.0002%, pH 7.0~7.2, tap water configuration.

[0055] B. trispora blakeslea trispora V-6515 was inoculated on a solid medium, the culture temperature was 28°C, and the culture time was 48 hours; the solid-cultured B. trispora blakeslea trispora The V-6515 culture was inoculated into t...

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Abstract

The invention discloses a lycopene high-yielding strain and application thereof. The strain is classified and named as blakeslea trispora V-6515 and has been preserved in China Center for Type Culture Collection CCTCC with an accession number of CCTCC No. M 2013583. According to the invention, nitrosoguanidine mutagenesis and flask fermentation are carried out to screen a stain with high yield of lycopene, the strain is used as an original strain in the next round of mutagenesis, and the target strain V-6515 is finally screened; the strain can greatly increase lycopene in fermentation products while reducing other components and has good genetic stability. The strain employs cassava powder as a carbon source for fermentation, and yield of lycopene produced through fermentation in a 5 L fermentor can reach 1.82 g / L, increased by 23.4% compared with the original strain VR-5(-); and the strain can be used in industrial production, greatly improves a fermentation unit and has a great economic value.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a lycopene high-yielding bacterial strain and its mutagenesis method and its application in fermentative production of lycopene. Background technique [0002] Carotenoids are a class of compounds that are abundant in fruits and vegetables and are the subject of extensive research due to their properties as antioxidants and vitamin A precursors. Lycopene has superior physiological functions, and its antioxidant effect is superior to that of β-carotene. It has the functions of anti-cancer, anti-cancer and activation of immune cells. It is widely used in food, medicine, cosmetics and health care industries. [0003] Lycopene is a carotenoid with the chemical formula C 40 h 56 , the molecular weight is 536.85. The molecular structure is as follows: [0004] Lycopene has chemical synthetic and natural sources. Chemical synthesis has been banned by most European and America...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P5/02C12R1/645
Inventor 姚忠泽
Owner CHANGZHOU BY YANG BIOLOGICAL ENG CO LTD
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