Bread yeast and application thereof in producing coenzyme I by fermenting
A technology of baker's yeast and coenzyme, which is applied in the direction of fermentation, microbe-based methods, microbes, etc., can solve the problems of no large-scale production, low yield and purity, etc., and achieve the effect of improving low fermentation yield and improving components
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Embodiment 1
[0037] Example 1 This example illustrates the method of mutagenizing the starting strain of baker's yeast to screen the target strain.
[0038] The baker's yeast SC-00 preserved in the laboratory was used as the starting strain for mutagenesis treatment, and the specific steps were as follows:
[0039]a) Preparation of single spore suspension: Take a cultured baker's yeast slant strain, add 10ml of sterile water, gently scrape off the spores on the surface of the agar, and place the spore suspension in a sterilized 50ml triangle In the bottle, put several sterile glass balls in advance, shake it well, filter it with sterilized absorbent cotton, and wash the filter residue with sterile water 2~3 times, finally make the volume of the filtrate reach 10ml, and count the filtrate with a hemocytometer , adjust the spore concentration to 1×10 6 a / ml;
[0040] b) Nitrogen ion implantation mutagenesis: take 100 μL of the spore suspension in step a), evenly spread it on a sterile empt...
Embodiment 2
[0048] This example illustrates the biological morphology and genetic stability of the mutagenic strain baker's yeast SC-Z180
[0049] Morphological and physiochemical properties of baker's yeast SC-Z180 of the present invention:
[0050] Colony color: milky white
[0051] Aerobic mode: facultative anaerobic;
[0052] Colony size: 1-4μm;
[0053] Growth temperature: 28-32°C;
[0054] Optimum pH: 5.4-6.5;
[0055] Colony shape: oval;
[0056] Mode of reproduction: budding;
[0057] Gram stain: Gram positive.
[0058] The results of the subculture fermentation test are shown in Table 1.
[0059] Table 1 Genetic stability of baker's yeast SC-Z180
[0060] Number of passages Coenzyme I production (g / L) 1 4.36 2 4.45 3 4.20 4 4.31 5 4.49 6 3.89
[0061] From the analysis of the results of the genetic stability experiment, it can be seen that after 6 subculture experiments, the coenzyme I produced by the fermentation of the muta...
Embodiment 3
[0063] This example illustrates the production process of coenzyme I high-yielding bacteria strain baker's yeast SC-Z180 fermented to produce coenzyme I
[0064] The culture medium formula described in the present embodiment is as follows:
[0065] Plate medium (g / L): glucose 20, fish powder peptone 20, agar 20, yeast extract 20, the rest is water, pH is natural.
[0066] Incline medium (g / L): glucose 20, fish powder peptone 20, agar 20, yeast extract 20, the rest is water, pH is natural.
[0067] Seed medium (g / L): Glucose 45, yeast extract 8, magnesium sulfate 0.1, ammonium sulfate 2, diammonium hydrogen phosphate 1, adjust the pH to 5.5 with 1mol / L hydrochloric acid, and dissolve the glucose.
[0068] Fermentation medium (g / L): glucose 50, yeast extract 10, magnesium sulfate 0.1, ammonium sulfate 2, diammonium hydrogen phosphate 1, tryptophan 3, adjust the pH to 5.5 with 1mol / L hydrochloric acid, and the rest is water, of which Glucose breakdown.
[0069] Saccharomyces c...
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