67 results about "Isocitric acid" patented technology

The invention relates to a composite oxide sensitive material used for monitoring formaldehyde and ammonia simultaneously. The composite oxide sensitive material is Au atom-doped La2O3-TiO2-SnO2 composite oxide nano-powder material which comprises the following components in mass percent: 3-5 percent of Au, 19-30 percent of La2O3, 33-45 percent of TiO2 and 24-31 percent of SnO2. A preparation method comprises the following steps: dissolving lanthanum salt, titanium salt and tin salt in an isocitric acid water solution, adding concentrated nitric acid and chloroauric acid in a defined amount, and performing back flow, volatilization, drying, calcination, grinding and calcination again to obtain the Au atom-doped La2O3-TiO2-SnO2 composite oxide nano-powder material. A formaldehyde-ammonia cataluminescence sensor manufactured by adopting the composite oxide sensitive material provided by the invention has wider linearity range, favorable selectivity and higher sensitivity, and can be used for performing on-line simultaneous monitoring of formaldehyde and ammonia in the air without being influenced by other concomitant substances.

The invention discloses an amphiphilic Eu (III) complex, a preparation method thereof and application thereof in sensing identification of citric acid/isocitric acid. The structural formula of the complex is shown in the specification; the complex is prepared by complexing a compound with lanthanide-series Eu (III), wherein the compound contain a double-alkyl-chain flexible tail group and a double pyridine carboxylic acid head group and serves as a ligand; the preparation method is simple and reaction conditions are mild. The amphiphilic Eu (III) complex has the characteristics that the fluorescent life is long, Stokes shift is large, and the coordination is unsaturated; the amphiphilic Eu (III) complex can be self-assembled in a water phase to form a vesicular supermolecule sensing interface with stable structure and uniform size, the vesicular supermolecule sensing interface is taken as a sensing platform, and by virtue of a synergetic complexation action between the Eu (III) complex assembled on the surfaces of vesicae and negative ions as well as the difference in capability of substituting water molecules bonded to Eu (III) ions by using carboxylic acid, the fluorescent sensitization degrees of the Eu (III) ions on the surface of the vesicae are differed, so that the high-sensitive and high-selectivity differentiation and identification for citric acid and isocitric acid and detection are achieved.

The invention discloses engineering bacteria for producing trans-4-hydroxy-L-proline and a construction method and an application thereof. The construction method of the engineering bacteria provided by the invention includes the steps of A1) and A2): A1) introducing a L-proline-4-hydroxylase gene, a glutamate-5-kinase gene and a glutamate-5-semialdehyde dehydrogenase gene into a receptor cell; and A2) knocking an alpha-ketoglutaricdehydrogenase gene, an isocitratlyase gene or a proline dehydrogenase gene of the receptor cell out, or replacing a pyruvic oxidase gene of the receptor cell with an acetylcoenzyme A synthetase gene; and carrying out a reaction of the recombinant cell and a substrate to obtain the trans-4-hydroxy-L-proline. Experiments prove that the production method of the trans-4-hydroxy-L-proline can be used for production of the trans-4-hydroxy-L-proline.

This invention relates to methods for increasing carbon fixation and/or increasing biomass production in a plant, comprising: introducing into a plant, plant part, and/or plant cell one or more heterologous polynucleotides encoding polypeptides having the enzyme activity of succinyl CoA synthetase, 2-oxoglutarate:ferredoxin oxidoreductase, 2-oxoglutarate carboxylase, oxalosuccinate reductase and isocitrate lyase to produce a stably transformed plant, plant part, and/or plant cell expressing the one or more heterologous polynucleotides. The methods further comprise introducing into a plant, plant part or plant cell heterologous polynucleotides encoding polypeptides having the enzyme activity of glyoxylate carboligase and tartronic semialdehyde reductase, and/or heterologous polynucleotides encoding a superoxide reductase from an archaeon species, an aquaporin and/or an inhibitor of cell wall invertase inhibitor. Additionally, transformed plants, plant parts, and/or plant cells are provided as well as products produced from the transformed plants, plant parts, and/or plant cells.

A method of sterilizing a material, said method comprising the steps of: a) providing a sterilizing composition comprising (i) peracetic acid and (ii) a stabilizer selected from the group consisting of citric acid, isocitric acid, aconitic acid and propane-1,2,3-tricarboxylic acid; b) introducing such sterilizing composition into a hot gaseous stream to produce a peracetic acid vapor; and c) contacting such peracetic acid vapor with the material to be sterilized. The use of such an organic acid stabilizer results in an unexpected reduction in the amount of residue deposited on the heating surface employed to vaporize the sterilizing composition.

The invention discloses a genetic engineering bacterium for producing glycollic acid by acetic acid, and a building method and application thereof. A method for preparing the genetic engineering bacterium for producing glycollic acid provided by the invention comprises the following steps of improving the expression and/or activity of acetylcoenzyme A synthetase, phosphotransacetylase, acetokinase, citrate synthase, isocitrate lyase, isocitrate dehydrogenase kinase and glyoxylate reductase in the recipient bacterium; reducing the expression and/or the activity of malate synthase, glycolate oxidase and isocitrate lyase repressor protein in the recipient bacterium, so that the engineering bacterium for producing the glycollic acid is obtained. The recipient bacterium is a bacterium capable of using acetic acid as a carbon source for growth. The prepared genetic engineering bacterium uses acetic acid as the carbon source for producing the glycollic acid; in addition, the production quantity and yield of the glycollic acid in shaking culture can reach the relatively high level; relatively good industrial application prospects are realized.

The invention relates to a highly selective sensitive material for trimethylamine and hydrogen sulfide, which is characterized in that the material is the composite powder material composed of Dy2O3,MoO3 and ZrO2. The preparation method comprises the steps of uniformly mixing acetic acid aqueous solution of dysprosium salt and isocitric acid aqueous solution of zircon salt; directly adding ammonium molybdate crystals into the mixture; adding carboxymethylcellulose to form a thick colloidal solution; and aging, drying, calcinating at two stages and naturally cooling to acquire the composite powder material composed of Dy2O3, MoO3 and ZrO2. A gas sensor manufactured by using the sensitive material provided by the invention can measure the trace trimethylamine and hydrogen sulfide in the airwith high sensitivity on the scene without being interfered by other common coexisting molecules.

The present invention discloses the signaling pathway involved in erythroid repression by iron deficiency. Further disclosed is a non-toxic small-molecule compound which potently reverses the erythroid repression caused by iron deficiency. The present invention further encompasses novel compounds for inhibition of red cell production, useful, for example, in the treatment of polycythemia vera, a malignancy causing uncontrolled red cell production. These inhibitory compounds also promote megakary-ocytic lineage commitment and may therefore be useful for augmentation of platelet production. The present invention further discloses isocitrate reversal of iron deprivation.

The invention discloses a cold stamping die mold-releasing agent capable of improving the mold-releasing efficiency. The cold stamping die mold-releasing agent is prepared from the following components in parts by weight: 19 to 23 parts of phenyltrimethylsilane, 15 to 18 parts of methyltribromosilane, 10 to 15 parts of tetraethyl orthosilicate, 25 to 28 parts of trimethylsilanol, 0.1 to 0.2 part of sodium methallyl sulfonate, 0.4 to 0.6 part of sodium ethylenediamine propane sulfonate, 2 to 4 parts of sodium glycerophosphate, 0.2 to 0.4 part of cocoyl diethanolamide, 1 to 2 parts of 4-nonylphenol, 3 to 5 parts of isocitric acid trisodium salt, 120 to 130 parts of No. 46 machine oil and 200 to 250 parts of de-ionized water. The cold stamping die mold-releasing agent provided by the invention has excellent de-molding performance and no pollution; after the cold stamping die mold-releasing agent is used, the surface of a prepared casting is smooth and clean and has no air pores; the abrasion of the casting and a die cavity can be extremely reduced, and the cavity is protected; the cold stamping die mold-releasing agent has the effects of lubricating and easily de-molding the casting.

The invention discloses biomarker compositions, and provides biomarker compositions for diagnosing cervicitis and a cervical cancer. The biomarker compositions are characterized by at least comprising one or a combination of uridine-5'-monophosphate disodium, histamine, 3'-O-methyl-guanosine and phenyllactic acid. The biomarker composition for diagnosing the cervicitis is characterized by comprising one or a combination of uridine-5'-monophosphate disodium, L-cysteine, isocitric acid, uridine diphosphate glucose, adenosine monophosphate, inosine 5'-monophosphate, L-pipecolic acid, N-acetyl putrescine and saccharose. The biomarker composition for diagnosing the cervical cancer is characterized by comprising one or a combination of histamine, phenyllactic acid, citraconic acid, L-2-aminoadipic acid, 3'-O-methyl-guanosine, beta-D-glucosamine, glycerin and cholesterol sulfate.

The invention discloses a preparation method of a semi-finished vegetable product. The method is carried out according to the following steps: selecting fresh vegetables, and bleaching the vegetablesfor 1 to 10 minutes in an isocitric acid trisodium salt solution or a sodium carbonate solution with a mass concentration of 0.01-0.05% at 85-100 DEG C; and bleaching the vegetables for 0.5-2 minutesin a sorbic acid solution or a sorbate solution with a concentration of 0.08-0.1%, then drying the bleached vegetables at 50-105 DEG C with a desiccant until dry matter content is 40-60%, and finallycooling the dried vegetables to 18-20 DEG C. The preparation method of the invention expands the varieties of the semi-finished vegetable product, obtains the semi-finished product with high dry matter content, improves the sensory performance and technical performance of the product, reduces the time for preparing the semi-finished product into a finished product and a final dish, enables the product to have a natural saturated raw material taste, and increases shelf life.

The invention relates to the field of an antirust paint, in particular to an antirust paint added with magnet powder for food production machine for a food production machine. The antirust paint is prepared from the following raw materials in parts by weight: 30 to 32 parts of acrylic emulsion, 4 to 6 parts of dimethyl silicone oil, 6 to 10 parts of oxaloacetic acid, 4 to 6 parts of isocitric acid, 4 to 6 parts of cyclohexanamine benzoate, 10 to 16 parts of boric acid, 1 to 2 parts of medical chalk ash, 1 to 2 parts of dibenzothiazyl disulfide, 1.5 to 1.5 parts of catechin and 3 to 5 parts of modified magnet powder. The antirust paint provided by the invention has the advantages of non-toxic and antibacterial properties, acid and alkali and salt spray corrosion resistance, low kinematic viscosity, close polymerization between raw materials, very high surface hardness after drying, scratch and impact resistance, oil and water immersion resistance, no peeling and bubbling in long-term oil and water immersion, and service life 3 to 4 years longer than the ordinary antirust paint.

The invention discloses an engineering bacterium lacking organic acid production paths and use thereof in co-production of 1,3-propanediol, 2,3-butanediol and ethanol. Lactic dehydrogenase gene of lactic acid synthesis path of Klebsiella, acetate kinase gene and pyruvic oxidase gene in acetic acid synthesis path, and fumarate reductase gene and isocitrate lyase gene of succinic acid synthesis path are inactivated, so that production of organic acids in metabolism of glycerol of the Klebsiella can be reduced, and yield and conversion rate of alcohols such as1,3-propanediol, 2,3-butanediol and ethanol can be improved, and the co-production of the 1,3-propanediol, 2,3-butanediol and ethanol can be achieved. As a significant reduction in the production of the organic acids, the extraction process of the alcohols can be simplified. The production efficiency of production of high value products by microbial conversion of glycerol can be improved, the production cost is reduced, and the engineering bacterium has important application value.

The invention discloses a derivative kit and method for separating a citric acid and an isocitric acid, and belongs to the field of chemical analysis. The kit comprises a derivatization reagent, the derivatization reagent is C3-C6 alcohols, namely methanol, ethanol, propanol, butanol, pentanol or hexanol, and further, the derivatization reagent is normal alcohol or isomeric alcohol. After the citric acid and the isocitric acid in a sample are derivatized by utilizing the kit disclosed by the invention, the separation of the citric acid and the isocitric acid is further realized by virtue of a liquid chromatography-mass spectrometry technology so that the subsequent analysis or detection is facilitated. By utilizing the kit and the method provided by the invention, the content of the citric acid can be accurately quantified by detecting a urine sample of a subject so that the accuracy and the sensitivity of urinary calculus diagnosis are improved, and the kit and the method have important clinical significance and a huge economic value.

The invention pertains to the porcine marker-assisted selection technical field. A genetic mark which is suitable for detecting porcine productive trait is cloned by the invention. The genetic mark is porcine isocitric acidcitric dehydrogenase gene IDH3Gamma, and cDNA sequence of the gene is stated in sequence table SEQID NO: 1. the sequence length is 1346bp, containing an open reading frame of 1179bp, an untranslated region of 36bp5' and a 3' untranslated region of 131p. The nucleotide sequences of IDH3Gamma gene such as SEQID NO: 3, SEQID NO: 4, SEQID NO: 5 and SEQID NO: 6 contain a second intron sequence of IDH3Gamma, and provide a new genetic mark the porcine marker-assisted selection. The invention also discloses a cDNA sequence of porcine IDH3Gamma gene and DNA sequence of the second intron for application of the pig microsatellite typing test.

The present invention discloses the signaling pathway involved erythroid repression by iron deficiency. Further disclosed is anon-toxic small-molecule compound which potently reverses the erythroid repression caused by iron deficiency. The present invention further encompasses novel compounds for inhibition of red cell production, useful, for example, in the treatment of polycythemia vera, a malignancy causing uncontrolled red cell production. These inhibitory compounds also promote megakaryocytic lineage commitment and may therefore be useful for augmentation of platelet production. The present invention further discloses isocitrate reversal of iron deprivation.

The invention discloses a chelation stabilizer suitable for a liquid-phase oxidation process. The stabilizer is prepared from a free radical scavenger, an antioxidant and a synergist in a compounding mode. The free radical scavenge is one or more of benzoate, phenylacetate and salicylic acid; the antioxidant is one or more of ascorbic acid, scorbic palmitate, erythorbic acid and sodium erythorbate; the synergist is prepared from citric acid and long-chain or branched-chain organic acid of isocitric acid. The chelation stabilizer can relieve degradation of a chelating agent in the desulfuration process, reduce chelating agent consumption and lower the operating cost of a desulfuration device.

The invention relates to an etching solution and application thereof. The etching solution comprises water, chloride, inorganic acid, organic acid and an oxidizing agent; the chloride is selected from at least one of sodium chloride, potassium chloride, copper chloride, ammonium chloride and hydrogen chloride; the inorganic acid is selected from at least one of nitric acid, sulfuric acid, phosphoric acid and perchloric acid; the organic acid is selected from at least one of iminodiacetic acid, formic acid, acetic acid, butyric acid, citric acid, isocitric acid, oxalic acid and malonic acid; the oxidizing agent is selected from at least one of hydrogen peroxide, sodium persulfate, ammonium persulfate, potassium persulfate, ammonium ceric nitrate, sodium ceric nitrate, potassium ceric nitrate, sodium hypochlorite and potassium hypochlorite; and the molar ratio of the chloride to the inorganic acid to the oxidizing agent is (55-70): (30-50): 1. The etching solution can quickly etch nickel, chromium or nickel-chromium alloy, has a small etching effect on copper, meets the process requirements of manufacturing fine lines in flexible printed circuit boards and integrated circuits at present, and is safe and reliable.

The invention discloses a beauty mask. The beauty mask comprises the following raw materials by weight parts: 2-5 parts of pearl extracts, 4-6 parts of milk essence, 4-9 parts of calabash extracts, 1-3 parts of isooctanol, 4-7 parts of frankincense extracting solution, 1-2 parts of isocitric acid, 2-4 parts of triglyceride, 1-4 parts of glycerol tripalmitate and 3-6 parts of deionized water. The beauty mask, provided by the invention, not only can effectively preserve moisture and moisten skin, tighten and lift, whiten skin and reduce speckle, resist crease and delay aging, diminish inflammation and expel toxin and prevent and cure pimple, and but also can adjust facial glossiness, keep the skin tender and moist and comprehensively beautify face.

The invention discloses an Escherichia coli strain for producing succinic acid with glycerol as well as a construction method and use. The Escherichia coli strain for producing succinic acid with glycerol has the preservation number of CGMCC No. 7999. The construction method comprises the following steps: knocking out the phosphoenolpyruvate carboxylase gene of Escherichia coli, and performing over-expression of key enzymes (isocitrate lyase and malate synthetase) of the glyoxylate cycle to obtain an evolved starting strain; by virtue of evolution, obtaining an evolved strain which can grow in an M9 basic salt medium with glycerol as unique carbon source and has enhanced glyoxylate cycle flux; knocking out the succinate dehydrogenase gene, and performing over-expression of phosphoenolpyruvate carboxylase and alpha-ketoglutaric dehydrogenase systems to obtain an Escherichia coli strain for producing succinic acid by using glycerol. The strain grows rapidly and has a high cell density, so that the rate of producing succinic acid with glycerol is increased.

The invention particularly relates to a wet electronic chemical silicon-based material etching solution. The solution comprises the following components in parts by weight: 25-45 parts of methanesulfonic acid; 10-30 parts of fluoride; 30-60 parts of a water-soluble acid; 0.5-9.5 parts of a complexing agent; 0.5-3.5 parts of a wetting agent; and the balance of water which makes the total parts of the components be 100 parts. The water-soluble acid comprises at least one of nitric acid, sulfuric acid, phosphoric acid, perchloric acid, sulfinic acid, formic acid, acetic acid, citric acid, isocitric acid and glycolic acid. The fluoride comprises at least one of trifluoromethanesulfonic acid, sodium fluoride, sodium hydrogen fluoride, ammonium fluoride, ammonium hydrogen fluoride, ammonium fluoborate, potassium fluoride, potassium hydrogen fluoride, aluminum fluoride, fluoboric acid, lithium fluoride, potassium fluoborate and calcium fluoride. The wet electron chemical silicon-based material etching solution can be used for safely, stably and efficiently etching a silicon substrate, can well solve the problem of insoluble byproducts generated in the etching process, has lower industrial safety risk in the production process, and lowers the cost required in a wastewater treatment procedure.

It was discovered that a compound of general formula (I) that has an isoxazole skeleton has an excellent inhibitory activity on a mutated IDH1 protein, inhibits 2-HG production by the aforesaid protein and can effectively inhibit the proliferation of various tumors expressing the aforesaid protein. In general formula (I), R1, R2, R3, Y and Z are each as defined in claim 1.