13 results about "Isocitric acid" patented technology

The application relates to an engineering bacterium for high yield of observation blue and a construction method and application thereof, and belongs to the technical field of biological synthesis of natural dyes. The engineering bacterium for high yield of observation blue expresses icd, bpsA, glnA Y405F and gdhA; the engineering bacterium knocks out acnR, yggB, glsK, aceA and ldh. By knocking out the yggB and aceA genes, performing site-directed mutation (Y405F) on the glnA gene, replacing the glsK gene with the glnA Y405F gene, replacing the acnR gene with the icd gene, replacing the ldh gene with the gdhA gene, and integrating the bpsA gene, the application can block the formation of by-products such as lactate and succinic acid in the observation blue synthesis process, improve the flow direction of the citric acid->isocitric acid->alpha-ketoglutaric acid->glutamic acid->glutamine path, and then improve the intracellular glutamine concentration, so that the yield of observation blue is improved.

The invention relates to the field of synthetic biology, metabolic engineering and fermentation engineering, and discloses a genetically engineered bacterium and application thereof in production of alpha-ketoglutaric acid.The genetically engineered bacterium comprises the steps that the genetically engineered strain with escherichia coli BW25113 or corynebacterium glutamicum ATCC13032 as a host is constructed; overexpression type isocitrate dehydrogenase genes are integrated through chromosomes, alpha-ketoglutarate dehydrogenase complex genes are knocked out, NADPH invertase is overexpressed through plasmids, and exogenous AKG transporter protein is introduced, so that metabolic flux directional regulation and control and product efflux enhancement are realized; meanwhile, a staged fermentation process is adopted and comprises dynamic gradient adjustment of inorganic phosphorus concentration and two-stage pH control. According to the method, the AKG yield of 78.2 g / L can be obtained under the 5L tank scale, the sugar acid conversion rate reaches 0.68, and the method has the advantages of being high in yield, low in byproduct and controllable in cost. The obtained AKG is suitable for a plurality of fields such as medical intermediates, functional foods and cell culture additives, and has wide industrial application prospects.

The present application belongs to the technical field of fermented fruit juice, and particularly relates to a method for preparing high-quality probiotic fermented strawberry juice based on magnetic field assistance. The present application first uses Lactobacillus plantarum CTCF-LP-1 to perform static fermentation on the strawberry juice to be fermented in a fermentation device for more than 24 hours, and then places the fermentation device in a static magnetic field. The static magnetic field reduces the generation of organic acid components by Lactobacillus plantarum during the fermentation process of the strawberry juice, reduces the acidity, and improves the fermentation quality of the strawberry juice. The magnetic field strength of the static magnetic field is 1.0-2.5 mT, and the fermentation time in the static magnetic field is 0.5-1.5 h. After the static fermentation and the magnetic field treatment, the content of malic acid, isocitric acid, glutaconic acid and other organic acid components in the fermented strawberry juice is significantly reduced, while the content of alcohol and ester components such as n-octanol, 2-phenylethanol, methyl benzoate, 3-hexyl hexanoate and butyl valerate is increased, the types of aroma components are more abundant, and the taste and quality of the strawberry juice are significantly improved.

The application discloses a genetically engineered bacterium for efficiently producing itaconic acid by using corn stalk hydrolysate and application thereof, and belongs to the technical field of gene recombination and metabolic engineering. The genetically engineered bacterium for efficiently producing itaconic acid by using corn stalk hydrolysate is characterized by the following aspects: taking a strain BW08 as a starting strain, heterologously expressing a homocentric acid decarboxylase gene CAD, deleting an isocitric acid dehydrogenase gene Icd, heterologously expressing a xylose acid dehydrase gene XD, a 2-keto-3-deoxy-xylose acid dehydrase gene KDXD and an alpha-ketoglutaric acid semialdehyde dehydrogenase gene KGSADH, and heterologously expressing a citric acid synthase gene gltA. In the fed-batch fermentation, the yield of itaconic acid of the genetically engineered bacterium reaches 68.6 g / L, and the conversion rate reaches 0.6 g / g (total sugar). The genetically engineered bacterium can efficiently metabolize corn stalk hydrolysate to produce itaconic acid.

This invention relates to a method for preparing and applying a tensile-strength copper-nickel alloy foil, belonging to the field of lithium battery materials technology. The invention synthesizes phenylboronic acid-modified isocitric acid with branched carboxyl and boric acid groups, and amine-modified sulfonic acid with sulfonic acid and amine groups. Utilizing the synergistic effect of these two active materials, the electrodeposition effect of nickel, copper, and neodymium in the electrolyte on the cathode plate can be effectively improved, reducing the electrodeposition grain size of the metal elements, increasing the uniformity of metal element distribution in the alloy foil, reducing the surface roughness of the metal foil, and improving the thickness uniformity and mechanical properties of the alloy foil. The copper-nickel alloy foil prepared by this invention has good strength and toughness, with a tensile strength of 1288~1351 MPa and an elongation of 5.8~6.2%, and also exhibits good appearance and high surface quality.

The invention relates to the technical field of biological medicine, and provides a plasma metabolite-based ALC and AH noninvasive differential diagnosis model, which is characterized in that five plasma key metabolites are taken as core indexes, and a probability calculation model is constructed through a logistic regression algorithm; the five kinds of plasma key metabolites are respectively an Ab-fubinaca metabolite 3, isocitric acid, N-docosahexaenoyl cysteine, gluconic acid-delta-lactone, and 1-palmitoyl-2-linoleyl phosphatidylethanolamine. The invention further provides a construction method and application of the model. According to the method, five key plasma metabolites with remarkable difference between AH and ALC are screened, modeling is carried out in combination with a logistic regression algorithm, or a multi-parameter model is constructed by further integrating prothrombin time, so that accurate non-invasive efficient identification of AH and ALC is realized.

The invention provides a method for improving the in-vitro maturation effect of vitrified bovine GV-stage oocytes, and in the cryopreservation process of the bovine GV-stage oocytes, the developmental ability of the bovine GV-stage oocytes after vitrification is improved by independently or jointly adding ergothioneine (EGT), isocitric acid (ICA), PLCzeta and Scriptaid. Experiments show that by adding EGT and ICA into a pretreatment solution, a freezing solution, an unfreezing solution and an oocyte in-vitro maturation solution and adding PLCzeta and Scriptaid into the unfreezing solution and the oocyte in-vitro maturation solution, damage of freezing to oocytes can be relieved to a great extent.

The application discloses a high-yield L-homoserine Corynebacterium glutamicum mutant strain and application thereof, and belongs to the technical field of fermentation engineering. The application takes Corynebacterium glutamicum ATCC13032 as a starting strain, knocks out the regulatory protein McbR, amino acid intracellular transport protein MetD, homoserine kinase, phosphoenolpyruvate carboxykinase, cysteine beta cleavage enzyme, O-acetylhomoserine sulfhydrylase, weakens isocitric acid dehydrogenase and dihydrodipicolinic acid synthase, and overexpresses pyruvate carboxylase gene mutant, aspartate kinase mutant, aspartate semialdehyde dehydrogenase, homoserine dehydrogenase, branched-chain amino acid transport protein BrnF, branched-chain amino acid transport protein BrnE, aspartate aminotransferase, bifunctional aspartate kinase / homoserine dehydrogenase 1 mutant, NADP + dependent glycerol-3-phosphate dehydrogenase. The recombinant bacteria provided by the application can reach 63.5g / L of L-homoserine yield after 96h of culture in a fermenter.

This invention discloses the application of isocitric acid in the control of bacterial wilt in plants, wherein the isocitric acid is derived from tomato root exudates. The inhibitory effect of isocitric acid on *Ralstonia solanacearum* was evaluated through shake-flask culture experiments and plate inhibition zone experiments, and the control effect of isocitric acid on bacterial wilt was determined using greenhouse pot experiments. The results showed that within the concentration range of 10–200 μM, isocitric acid significantly inhibited the growth of *Ralstonia solanacearum*, forming a distinct inhibition zone. Under greenhouse pot conditions, applying 0.2–0.5 mg / kg of isocitric acid to the soil effectively reduced the incidence of bacterial wilt in tomatoes, with a disease control rate of 21.43%–52.38%. Furthermore, isocitric acid can indirectly inhibit the occurrence of bacterial wilt by influencing the soil bacterial community. The isocitric acid used in this invention is derived from natural plant root exudates, possessing advantages such as environmental friendliness and high safety, and can be used as a novel inhibitor of tomato bacterial wilt, showing promising application prospects in the field of green control of plant diseases.

The application provides a CO2 capture method and a CO2 capture device coupled with electrochemical regeneration of hydrogen. The method comprises the following steps: absorbing CO2 in a CO2-containing gas by using an alkaline solution to form a CO2-rich solution; performing acid-base neutralization of the CO2-rich solution and an acidic solution to obtain a neutralization solution and CO2; performing electrolysis of the neutralization solution to obtain a cathode electrolysis product and an anode electrolysis product, wherein the cathode electrolysis product is H2 and the alkaline solution, and the anode electrolysis product is O2 and the acidic solution; and the acidic solution is selected from one or a combination of more than two of the following solutions: pyruvic acid solution, gluconic acid solution, malonic acid solution, tartaric acid solution, glyceric acid solution, oxalacetic acid solution, formic acid solution, isopropionic acid solution, isobutyric acid solution, fumaric acid solution, aconitic acid solution, isocitric acid solution and hexanediol diacid solution. The acidic solution generated by electrolysis can avoid corrosion of the device equipment and reduce the net energy consumption and net cost of the CO2 capture process.

The present invention makes it possible to improve the production yield of 3-hydroxyhexanedioic acid, (E)-hex-2-enedioic acid, and / or hexanedioic acid while reducing the by-production of acetic acid by enhancing the function of isocitrate lyase in a microorganism capable of producing 3-hydroxyhexanedioic acid, (E)-hex-2-enedioic acid, and / or hexanedioic acid. The present invention further makes it possible to improve the production yield of 3-hydroxyhexanedioic acid by increasing the expression level of YnfM or a homolog thereof in a microorganism capable of producing 3-hydroxyhexanedioic acid.

The objective is to improve the productivity of 3-hydroxyadipic acid, α-hydromuconic acid, and / or adipic acid while reducing the by-product of acetic acid in a method for producing 3-hydroxyadipic acid, α-hydromuconic acid, and / or adipic acid using microorganisms. [Solution] A genetically modified microorganism capable of producing 3-hydroxyadipic acid, α-hydromuconic acid, and / or adipic acid, in which the function of isocitrate lyase is enhanced.

The object of the present invention concerns a hair restructuring association comprising or alternatively consisting of: (a) a quaternary ammonium salt of formula (I) and (b) at least one sulpho-derivative of vegetable fatty acids, wherein R5, R6 are independently chosen between hydrogen and a radical R0, R0 consists of the following structure of formula (II), R1 is chosen from the group consisting of: hydrogen, methyl, isopropyl, sec-butyl, isobutyl, ethylenemethylthio, benzyl, para-hydroxybenzyl and 3-methylene-1H-indole, R2, R3, R4 are independently chosen from the group consisting of: methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl and tert-butyl, mX− is chosen from the group consisting of: formic acid, acetic acid, unsaturated monocarboxylic acids, adipic acid, aldaric acid, oxalic acid, phthalic acid, azelaic acid, sebacic acid, malonic acid, succinic acid, tartaric acid, glutaric acid, pimelic acid, maleic acid, malic acid, fumaric acid and suberic acid, isocitric acid, citric acid, fatty acids, acidic amino acids, keto acids and aromatic carboxylic acids, m is an integer number comprised between 1 and 22, n is comprised between 2 and 20.