14 results about "Pipecolic acid" patented technology

The invention relates to the technical field of plant disease prevention and treatment, in particular to application of externally sprayed L-piperidine acid as a disease-resistant activator in improving rice blast resistance, L-piperidine acid is applied to rice blast prevention and treatment for the first time, an efficient Pip treatment scheme and precise application time are established for a susceptible variety Lijiang Xingroup black grain, and the disease resistance of rice is improved. Based on a magnaporthe oryzae infection process, determining 10 hpi (after inoculation per hour) after inoculation as an optimal spraying window period; pip is a natural metabolite of plants, has no residual risk, and meets the requirements of green agriculture; and a defense signal induced by Pip can be continued to the later growth period of the rice, and the yield and the quality are not influenced.

The present specification relates to a composition including a new amide-based compound derived from an amino acid structure such as valine, leucine, phenylalanine, proline, pipecolic acid, or the like, wherein the composition exhibits a skin anti-aging or skin reverse-aging effect by restoring the size and number of dendrites of aged melanin-producing cells to those of young cells. As a result, the composition may exhibit a skin brightening effect by inhibiting the amount of melanin produced in the melanin-producing cells.

Disclosed is a method for preparing (S)-1,2,3,4-tetrahydroisoquinoline-1-carboxylic acid and derivatives thereof, comprising: taking a racemate of a compound represented by Formula (I) or a racemate of a salt of the compound represented by Formula (I) as a substrate, and making a R-isomer of the compound represented by Formula (I) in the substrate react under the catalysis of oxidative dehydrogenase to generate imino acid represented by formula (II); and converting the imino acid represented by Formula (II) into an S-isomer of the compound represented by Formula (I) in the presence of pipecolic acid reductase and a coenzyme capable of supplying hydrogen anions. The process has mild reaction conditions, strong stereoselectivity, high reaction efficiency, and high conversion rate.

The invention provides a colletotrichum gloeosporioides gene deletion type strain and a huperzine A biosynthesis method. A CAO3097 gene is deleted in a genome of the strain. According to the method, a CAO3097 gene is knocked out from colletotrichum gloeosporioides by utilizing a genetic engineering means, a gloeococcus gloeosporioides culture solution is added in the culture process of colletotrichum gloeosporioides, and the capability of synthesizing huperzine A by colletotrichum gloeosporioides is obviously improved by adopting a method of applying piperidine acid. According to the method, a genetic engineering means is adopted for the first time, and the efficiency of microbial fermentation of huperzine A is improved in combination with innovation of a culture process.

The invention discloses streptomyces hygroscopicus ascomycetes subspecies and an obtaining method thereof. Regulating genes fkbN, fkbR1 and fkbR2 on an ascomycin biosynthesis gene cluster of the streptomyces hygroscopicus ascomycetes subspecies ATCC 14891 and a gene fkbS participating in synthesis of an ascomycin precursor ethyl malonyl coenzyme A are subjected to biosynthesis to obtain the streptomyces hygroscopicus ascomycetes subspecies. And carrying out overexpression on a regulatory gene SH03686 of a leucine response regulatory protein family (Lrp) outside the gene cluster, and screening an ascomycin high-yield strain. According to the invention, after combined overexpression of genes fkbS and fkbN and overexpression of a gene fkbR2 are carried out by a strain SN9 and a strain R2-17 in starting bacteria, the transcriptional level of regulatory genes (fkbN, fkbR2) on an ascomycin biosynthesis gene cluster and related genes participating in synthesis of precursors ethyl malonyl coenzyme A (fkbS, fkbE), piperidine acid (fkbL) and DHCHC (fkbO) are improved, and finally, the ascomycin yield is improved.

Pipecolic acid-based surfactants and their use This invention deals with pipeolic acid-based surfactants and their uses, for example in laundry or dishwashing.

The invention provides a composition comprising oleanolic acid and the salts, prodrug and solvates thereof; and one or more compounds selected from the following group: ellagic acid, adenine, betaine, choline, miquelianin, p-coumaric acid, pipecolic acid, protocatechuic acid and tyramine, and the salts, prodrugs and solvates thereof. Such a composition is for use in reducing lipid levels in sebocytes, and the composition may find use in treating acne vulgaris and rosacea.

This specification relates to novel amide compounds derived from amino acid structures such as valine, leucine, phenylalanine, proline, or pipecolic acid, which have MMP-1, MMP-2, or MMP-9 inhibitory effects even in trace amounts, their isomers, pharmaceutically acceptable salts thereof, their hydrates, or solvates, and compositions containing the same.

This specification relates to compositions comprising novel amide compounds derived from amino acid structures such as valine, leucine, phenylalanine, proline, or pipecolic acid. [Solution] The composition can exert an immune-enhancing effect by activating the phagocytic activity of macrophages, which are important components of the human immune system and help eliminate pathogens.

The present invention addresses the problem of providing: a mutant L-pipecolic acid hydroxylase for producing cis-5-hydroxy-L-pipecolic acid in a highly productive and highly selective manner by hydrating L-pipecolic acid; and a novel method for industrially producing cis-5-hydroxy-L-pipecolic acid from L-pipecolic acid in a highly productive and low-cost manner. The present invention provides a mutant L-pipecolic acid hydroxylase that has an amino acid sequence in which a specific amino acid mutation is introduced in the amino acid sequence represented by SEQ ID NO: 2.

The present disclosure belongs to the technical field of enzyme engineering and genetic engineering, and relates to dioxygenase mutants and use thereof in synthesizing (2S,3R)-3-hydroxypipecolic acid. By mutating arginine at position 97 and asparagine at position 43 of the L-proline-3-hydroxylase, the present disclosure makes the specific activity of R97M / N43T up to 2.42 times that of wild-type (WT) enzyme; after conditions are optimized, R97M / N43T achieves a molar yield of 8.82 mM at a substrate concentration of 10 mM under crude enzyme conditions, with a molar yield rate of 88%, which is 4.11 times that of the wild-type enzyme, and a yield of (2S,3R)-3-hydroxypipecolic acid produced by R97M / N43T catalyzing the L-pipecolic acid is 1278 mg·L−1. The mutant with improved catalytic efficiency toward the L-pipecolic acid in the present disclosure is more suitable for research on the production of (2S,3R)-3-hydroxypipecolic acid.

The present invention addresses the problem of providing a stable hydroxylase having high L-pipecolic acid hydroxylation activity and high stability by introducing a mutation into an L-pipecolic acid hydroxylase.　It has been found that the effects of improving the L-pipecolic acid hydroxylation activity and stability of a hydroxylase (McPH) when the hydroxylase is expressed in a host such as E. coli can be obtained by identifying an amino acid residue that can affect an improvement in the hydroxylation activity and stability of the hydroxylase, and introducing a mutation into the identified amino acid residue.

The present invention addresses the problem of providing a hydroxylase having high L-pipecolic acid hydroxylation activity for efficiently producing high-purity hydroxy-L-pipecolic acid at low cost and with high productivity.　It has been found that the effects of increasing the yield of a solubilizing enzyme when a hydroxylase (McPH) is expressed in cells, improving the L-pipecolic acid hydroxylation activity of the enzyme itself, and reducing the activity of converting L-proline into hydroxy-L-proline are obtained by identifying an amino acid residue capable of affecting an improvement in the hydroxylation activity of the hydroxylase, and introducing a mutation into the identified amino acid residue.

A method for rapidly improving the salt tolerance of salt-sensitive rice belongs to the field of rice planting technology. The steps are as follows: Step 1. Dissolve DL-piperidinic acid with a 3-10 wt.‰ NaCl solution to prepare a DL-piperidinic acid solution with a concentration of 50-400 mg / L, and then disinfect the surface of the rice seeds; Step 2. Soak the disinfected rice seeds in the DL-piperidinic acid solution at a temperature of 20-30°C for 16-24 h to obtain treated rice seeds, so that they germinate and grow normally under a salt stress environment. The present invention utilizes exogenous DL-piperidinic acid to soak rice seeds and irrigate them, which can improve the germination rate, root length and growth rate of salt-sensitive rice seeds under salt stress conditions, improve the salt tolerance of salt-sensitive rice, meet the urgent demand of my country's coastal mudflats for high-yield, high-quality, disease-resistant, salt-tolerant, direct-seeding rice varieties, and improve saline-alkali land, with high application potential and value.