339 results about "Sphingosine" patented technology

Compositions and methods for producing monoclonal antibodies and their derivatives reactive against bioactive lipid targets are described. These compositions include derivatized lipids, each of which comprises a bioactive lipid that having a polar head group and at least one hydrocarbon chain (e.g., a lysolipid such as lysophosphatidic acid or sphingosine-1-phosphate) in which a carbon atom has been derivatized with a pendant reactive group; immunogens made by linking a derivatized lipid to a carrier moiety (e.g., a carrier protein, polyethylene glycol, colloidal gold, alginate, or a silicone bead); monoclonal antibodies and derivatives produced by immunizing an animal with such an immunogen; and therapeutic and diagnostic compositions containing such antibodies and antibody derivatives. Methods for making such derivatized lipids, immunogens, and monoclonal antibodies and derivatives, methods for detecting such antibodies once generated, and therapeutic and diagnostic methods for using such antibodies and derivatives, are also described.

The present invention relates to DNA segments isolated from Sphingomonas sp. and involved in the biosynthetic production of sphingan polysaccharides to increase the production of the polysaccharide in engineered microorganisms. The present invention also relates to methods of engineering strains of Sphingomonas to produce bacteria which are hyperproducers of sphingan, methods of identifying and utilizing DNA fragments useful to enhance production of sphingan in bacteria and the hyperproducer bacteria.

The invention relates to mutant strains of the genus Sphingomonas which have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate (“PHB”) synthesis that allows the mutant strains to produce PHB-deficient sphingans. The invention is also directed to a process for preparing a clarified sphingan solution comprising heating aqueous sphingan solution, in particular PHB-deficient sphingan solution, to a clarification temperature of about 30° C. to about 70° C., and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.

Described are methods for protecting the female reproductive system against natural and artificial insults by administering to women a composition comprising an agent that antagonizes one or more acid sphingomyelinase (ASMase) gene products. Specifically, methods disclosed herein serve to protect women's germline from damage resulting from cancer therapy regimens including chemotherapy or radiotherapy. In one aspect, the method preserves, enhances, or revives ovarian function in women, by administering to women a composition containing sphingosine-1-phosphate, or an analog thereof. Also disclosed are methods to prevent or ameliorate menopausal syndromes and to improve in vitro fertilization techniques.

In vitro/cell-free process of preparing a sialylated oligosaccharides are described. The sialylated oligosaccharides include gangliosides. The oligosaccharides linked to various moieties including sphingoids and ceramides. Novel compounds that comprise sphingoid groups are disclosed. The compounds include sialylated oligosaccharides including gangliosides as well as various sphingoids and ceramides.

The invention relates to a method for preparing phytosphingosine liposome composition having excellent long-term storage stability and the method comprises dispersing phytosphingosine in water, separately dissolving phospholipid in a solvent and mixing the two solutions prepared by the above procedures.

The present invention includes methods for the synthesis of sphingomyelins and dihydrosphingomyelins. The present invention also includes methods for the synthesis of sphingosines and dihydrosphingosines. The present invention further includes methods for the synthesis of ceramides and dihydroceramides.

The invention discloses an acne removing essence with added pearl hydrolyzate liposomes, which is prepared by mixing and stirring glycerol, 1,3-butanediol, ethylenediaminetetraacetic acid disodium salt, pearl hydrolyzate liposomes, plant sphingosine, dipotassium glycyrrhizate, imidazolidinyl urea and deionized water; the preparation process is that the glycerol, the 1,3-butanediol, the ethylenediaminetetraacetic acid disodium salt and the deionized water are mixed and evenly stirred, the pearl hydrolyzate liposomes, the plant sphingosine, the dipotassium glycyrrhizate and the imidazolidinyl urea are added for stirring after the heating, heat preservation and cooling, and the discharge obtains the finished product. The pearl hydrolyzate liposomes containing a variety of amino acids are added in the acne removing essence, which is not only anti-inflammatory, bactericidal and can remove acnes, but also can effective enhance the metabolic function of skin cells, dredge the pores of the skin cells and promote the regeneration of the surface cells, so the acne removing essence can effectively repair the damaged skin, accelerate the healing of damaged skin open sores and acne spots and ensuring the skin to be delicate, smooth, white and moist.

A method of treating a subject in need of a cell or tissue transplant is disclosed. The method comprising (a) transplanting a non-syngeneic cell or tissue transplant into the subject, wherein the transplant comprises bone marrow or lymphoid cells; and (b) administering to the subject a therapeutically effective amount of an immunosuppressive regimen comprising a Sphingosine 1-Phosphate Receptor Agonist, a B7 molecule inhibitor and a CD2/CD58 pathway inhibitor, thereby treating the subject.

The invention relates to a synergistic effect of multiple bacterial strains on degradation of polyphenol pollutants and a research method thereof. According to the invention, a bacterial suspension is prepared from one or a mixture of two or three selected from the group consisting of pseudomonas aeruginosa GIMT1.074, sphingobacterium multivorum CICC 23249 and ochrobactrum sp. BJS2; then the bacterial suspension is inoculated to inorganic a salt medium containing phenol, meta-cresol and 4-chlorophenol with different concentrations for culture; parts of a culture solution are taken out at different time intervals to measure concentrations of phenol, meta-cresol and 4-chlorophenol in the culture solution so as to determine degradation rates at different times, and cell concentrations in the culture solution are measured to investigate growth conditions of different cells. According to research on synergistic degradation effects of the three phenol degrading bacteria, a mixed bacterial strain has superior capability in degradation of mixed phenolic pollutants and has much better degradation efficiency and effects compared to every individual bacterial strain; moreover, biomass of the mixed bacterial strain is increased to two times of the biomass of an original optimal bacterial strain, i.e., the pseudomonas aeruginosa GIMT1.074.

The present invention provides methods for producing human ceramide in a yeast cell.

The methods of the present invention comprise:

• • 1) introducing the sphingoid Δ4-desaturase gene (DES1) by transformation of the yeast cell; and
  • 2) abolishing the expression of the yeast sphinganine C4-hydroxylase gene (SUR2) by transformation of the yeast cell.

To provide a fat accumulation inhibitor and a food or drink for inhibiting fat accumulation, a visceral fat accumulation inhibitor and a food or drink for inhibiting visceral fat accumulation, or an agent for accelerating increase and/or inhibiting decrease of an adiponectin concentration in blood and a food or drink for accelerating increase and/or inhibiting decrease of an adiponectin concentration in blood.

A fat accumulation inhibitor for a fat cell, which comprises a milk-derived phospholipid as an active ingredient, and a food or drink for inhibiting fat accumulation in a fat cell, which comprises a milk-derived phospholipid. A visceral fat accumulation inhibitor comprising a sphingosine-containing phospholipid or a derivative thereof as an active ingredient, and a food or drink for inhibiting visceral fat accumulation. An agent for accelerating increase and/or inhibiting decrease of an adiponectin concentration in blood, which comprises a sphingosine-containing phospholipid or a derivative thereof as an active ingredient, and a food or drink for accelerating increase and/or inhibiting decrease of an adiponectin concentration in blood.

The invention provides sphingobium yanoikuyae with a function of degrading triphenyl phosphate. The bacterium is named YC-XJ2, has a preservation number of CGMCC No.18049, and enables 100mg/L TPhP ininorganic salt to be degraded by 99.7 percent in 24 hours. The sphingobium yanoikuyae strain YC-XJ2 has strong adaptability on temperature, can keep the degradation rate on TPhP at 95 percent at 40 DEG C, can keep the degradation rate on TPhP at 88 percent or more when the pH value is 4-10, has a degradation at 74.8 percent or more when the salt concentration is 0-10 percent, and shows extremely strong salt resistance. The strain can be applied to biological repairing of a TPhP polluted environment and has relatively good economic value and application prospect.

The invention discloses a method for accelerating formation of a biofilm in waste water under a low temperature condition, and belongs to the technical field of waste water treatment. The method comprises the following steps: 1, amplification culture and low-temperature adaptation culture of a red sphingosine pure monad; 2, preparation of a low-temperature resistance immobilized red sphingosine monad pellet; 3, adding the prepared low-temperature resistance immobilized red sphingosine monad pellet, paddings, aerobic activated sludge and low-temperature waste water into an MBBR (moving bed biofilm reactor) and then performing post-aeration treatment; 4, keeping a corresponding aeration quantity and a corresponding pH value in the MBBR until the formation of the biofilm is completed. According to the method, low-temperature adaptation culture is performed on the red sphingosine monad, and the red sphingosine pure monad is added into the reactor for increasing bacterial extracellular polymers, so that microorganisms can grow on the paddings faster, and the microbial activity is improved. Therefore, the formation of the biofilm in the waste water under the low temperature condition is accelerated, and the formed biofilm is firm, strong in shock load resistance, and good in waste water treatment effect.

Disclosed herein are novel human nucleic acid sequences that encode polypeptides. Also disclosed are polypeptides encoded by these nucleic acid sequences, and antibodies that immunospecifically-bind to the polypeptide, as well as derivatives, variants, mutants, or fragments of the aforementioned polypeptide, polynucleotide, or antibody. The invention further discloses therapeutic, diagnostic and research methods for diagnosis, treatment, and prevention of disorders involving any one of these novel human nucleic acids and proteins.