330 results about "Sphingosine" patented technology

The invention relates to a preparation method and application of an artificially synthesized exosome with an anti-aging effect, and belongs to the technical field of cosmetics and biology. The artificially synthesized exosome is prepared from hydrogenated lecithin, phytosphingosine, cholesterol, ceramide, squalane, sodium stearoyl glutamate, a surface modification structure, polyol, an active component and water. The preparation method comprises the steps of preparation of an alcohol phase, preparation of a water phase, preparation of a mixed solution and high-pressure homogenization treatment. The artificially synthesized exosome prepared by the preparation method provided by the invention is good in stability and high in anti-aging activity.

The invention relates to a construction method of a multi-class lipid retention time general prediction model and a prediction system. The method comprises the following steps: by taking experimental retention time tRE of a lipid compound in a training set as a dependent variable and characteristic structure parameters of the lipid compound as an independent variable, carrying out quantitative processing on the independent variable and then carrying out regression modeling analysis, the characteristic structure parameters comprise the total carbon number c and the total carbon-carbon double bond number d of a fatty acyl chain, an ether chain, an alkenyl ether chain or / and a sphingosine skeleton, the types of skeletons contained in the lipid compound and the number of corresponding skeletons, and the types of characteristic groups and residues in the lipid compound and the number of corresponding residues; carrying out numerical quantization on parameters according to the number of skeletons, characteristic groups or residues of the corresponding types; the QSRR general prediction model of the retention time of the multi-class lipid compounds is obtained by adopting regression modeling, a more accurate MRM data acquisition window can be set for the lipid compounds, and the sensitivity, stability and coverage can be improved.

The invention relates to a bionic exosome for anti-inflammation and repair as well as a preparation method and application thereof, and belongs to the field of cosmetics. The bionic exosome provided by the invention comprises a membrane structure and an internal load phase, the membrane structure comprises a phospholipid bilayer, a stabilizer and a targeting ligand; the internal load phase comprises a solvent and an active component; the phospholipid bilayer is composed of phospholipid compounds; preferably, at least one of the distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 and the 1, 2-dioleoyl-sn-glycerol-3-phosphoethanolamine is adopted as the phospholipid compound, and at least one of the distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 and the 1, 2-dioleoyl-sn-glycerol-3-phosphoethanolamine is adopted as the phospholipid compound; at least one of cholesterol, phytosphingosine and sodium stearoyl glutamate is preferably adopted as a stabilizer, so that the stabilizer of the obtained bionic exosome is improved, and the bionic exosome has the advantages of high encapsulation efficiency, good encapsulation efficiency stability, simplicity in operation, excellent anti-inflammatory and repairing effects and the like.

The invention discloses a bacterial strain for degrading aspartame, a bacterial-algae symbiotic system and application of the bacterial strain and the bacterial-algae symbiotic system, the bacterial strain is corn sphingomonas zeae NCU J1, and the preservation number of the bacterial strain is CCTCC (China Center for Type Culture Collection) NO: M 20251462. And an algal-bacterial symbiotic system is constructed by adopting an oriented symbiotic combination of the corn sphingomonas NCU J1 and the chlorella sp. The corn sphingomonas NCU J1 can metabolize an artificial sweetening agent aspartame and degradation products thereof, metabolism complementation can be achieved through an algal-bacterial symbiotic interface, and bacteria preferentially promote aspartame to be subjected to photohydrolysis to generate degradation products such as diketopiperazine derivatives. The microalgae synchronously start isomerism enzymatic reaction to convert degradation products such as diketopiperazine derivatives into low-toxicity products, the risk of secondary pollution is reduced by 40% or above compared with that of a traditional physical and chemical method, 150 mg / L aspartame can be efficiently degraded within 2 days, generation of derivatives of aspartame can be greatly reduced, secondary pollution is reduced, and the method is suitable for industrial production. And the method has a good application prospect in the aspect of rapidly treating aspartame-containing wastewater.

Provided herein are lipid nanoparticles composition comprising a lipid component which comprises: (i) a first ionizable cationic lipid, (ii) a PEG-lipid, wherein the PEG-lipid comprises a PEG-Ceramide. Further provided are lipid nanoparticle compositions comprising a lipid component which comprises: (i) a first ionizable cationic lipid, (ii) a PEG-lipid, wherein the PEG-lipid comprises a PEG-Ceramide selected from: N-octanoyl-sphingosine-1-{succinyl[methoxy(polyethylene glycol)5000]} (C8 PEG5000-Ceramide), N-octanoyl-sphingosine-1-{succinyl[methoxy(polyethylene glycol)2000]}(C8 PEG2000-Ceramide), N-octanoyl-sphingosine-1-{succinyl[methoxy(polyethylene glycol)750]} (C8 PEG750-Ceramide), N-palmitoyl-sphingosine-1-{succinyl[methoxy(polyethylene glycol)5000]} (C16 PEG5000-Ceramide), N-palmitoyl-sphingosine-1-{succinyl[methoxy(polyethylene glycol)2000]} (C16 PEG200-Ceramide), and N-palmitoyl-sphingosine-1-{succinyl[methoxy(polyethylene glycol)750]} (C16 PEG750-Ceramide) (iii) a phospholipid, and (iv) a permanently cationic lipid, an anionic lipid, or a second ionizable cationic lipid separate from the first ionizable cationic lipid.

The invention relates to the technical field of kitchen waste treatment, in particular to a solid fungicide, a preparation method and application. The solid microbial inoculum comprises a microbial inoculum and a carrier; the microbial agent is prepared from pseudoochrobactrum saccharum, alcaligenes faecalis, achromobacter xylosoxidans, paenibacillus amyloliquefaciens, bacillus cereus, sphingobium nicotianae, serratia marcescens, stenotrophomonas maltophilia, sphingobium humus, sphingobium aquaticum and bacillus subtilis; the carrier for carrying the liquid compound bacteria is bagasse, and the maximum number of released bacteria is 2.2 * 10 < 9 > CFU / g. When the solid microbial inoculum is added into a 18 L kitchen waste reactor, the waste decrement rate is 80% within 7 days, and the COD removal rate can reach 79.1% at most. According to the solid microbial agent disclosed by the invention, organic substances in the kitchen waste can be degraded while the kitchen waste is converted from a solid state to a liquid state, so that volume reduction and weight reduction of the kitchen waste are realized; and the solid microbial inoculum is convenient to transport and operate and low in cost, has positive significance on difficulties faced by kitchen waste treatment, and has a good application prospect.

The invention discloses a natural component composition for conditioning skin micro-ecology, a preparation method of the natural component composition and application of the natural component composition in skin care products for children. The composition comprises a synergistic combination of a sphingomonas fermentation product extract, a haematococcus pluvialis extract rich in astaxanthin, an abelmoschus esculentus fruit extract rich in polysaccharides, and a yeast / zinc fermentation product. The present invention provides improvements in astaxanthin in terms of chemical stability and transdermal permeability. The composition aims to enhance the immature skin barrier, provide a good effect, and support the healthy development of skin microecology, thereby solving the unique physiological needs of children's skin. The invention also describes a preparation method of the composition and application of the composition in cosmetics for children and dermatological products.

The invention discloses an organic contaminated soil remediation method based on a compound bacterium remediation agent. The organic contaminated soil remediation method comprises the following steps: S1, biochar modification: FeCl3 and LaCl3. 7H2O modified biochar is obtained; s2, culturing compound bacteria, namely mixing the ectotrophic substance sphingosine bacterium suspension and the staphylococcus xylosus suspension in an equal volume manner to obtain a compound bacteria suspension, wherein the sphingosine bacterium suspension and the staphylococcus xylosus suspension are mixed in an equal volume manner; s3, immobilizing compound bacteria; and S4, soil remediation. Aiming at the soil jointly polluted by a plurality of organic polluted soils, two microbial bacteria which are relatively good in synergism and relatively good in degradation effect on organic pollutants are screened out as compound bacteria, and meanwhile, in order to further improve the action effect and activity of the compound bacteria, the compound bacteria are loaded on specific biochar through a series of means, so that the compound bacteria can be used for preparing the biochar. The activity can be further improved, so that the synergistic degradation of various organic pollutants in the soil is improved, and a good effect is achieved.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to rice endophytic sphingosine vector SY02 and application thereof. The preservation number of the rice endophytic sphingobium yanoikuyae SY02 provided by the invention is CCTCC (China Center For Type Culture Collection) NO: M2026182, and the sphingobium yanoikuyae SY02 has the functions of producing indoleacetic acid, promoting plant growth and seed germination, promoting adventitious bud proliferation of tissue culture seedlings and relieving browning. The sphingosine vector bacterium and the microbial agent prepared through strain fermentation have the advantages of being green, safe, efficient, long-acting and the like, and have good application prospects in the aspects of promoting plant growth and seedling breeding.

The invention relates to a polyacid composite oil-controlling and acne-removing gel and a preparation method thereof, and belongs to the technical field of skin care products. The polyacid composite oil-controlling and acne-removing gel is prepared from the following components in percentage by mass: 2% of salicylic acid, 3%-4% of glycolic acid, 0.5%-1.5% of mandelic acid, 0.5%-1.0% of lactobionic acid, 0.5%-1.0% of gluconolactone, 1.2%-1.6% of microcrystalline cellulose (and) sphingomonas fermentation product extract (and) cellulose gum, 2%-5% of butanediol, 0.3%-0.5% of asiaticoside-II, 0.5%-1.5% of centella asiatica, 0.5%-1.5% of vitamin E and the balance of water. And the balance of deionized water. By adding micro-crystalline cellulose (and) sphingomonas fermentation extract (and) cellulose gum, system stability under compounding of five organic acids is realized, semitransparent gelatinous texture is obtained, the composition is suitable for point coating use mode, impression is not affected, oil-controlling and acne-removing effects are improved under the condition of low organic acid content, and the composition belongs to skin-care polyacid products and has a broad market prospect. The mild oil control and acne removal effects are realized, and the cream is suitable for oil-sensitive skin crowds.

The invention discloses a bisphenol A degrading bacterium XYQ100 as well as a culture method and application thereof, and relates to the technical field of microbial pollution degradation and environmental pollution, the bisphenol A degrading bacterium XYQ100 provided by the invention is classified and named as sphingobacterium sp., and is preserved in the China General Microbiological Culture Collection Center on April 18, 2025, and the preservation number of the strain is CGMCC NO.34264. The bisphenol A degrading bacterium XYQ100 provided by the invention is named as sphingobacterium sp. The strain can realize efficient removal of bisphenol A pollutants, and the degradation rate of bisphenol A within 54 hours reaches 100%.

The invention belongs to the technical field of biology, and particularly relates to an immunogenic peptide fragment aiming at a human sphingosine 1-phosphate receptor 2 and application of the immunogenic peptide fragment. The amino acid sequence of the immunogenic peptide fragment is SEQ ID No.1. The immunogenic vector vaccine aiming at the human sphingosine 1-phosphate receptor 2 is prepared by coupling the immunogenic peptide fragment of the human sphingosine 1-phosphate receptor 2 and a vector, and the vector is Q beta-2aa bacteriophage virus-like particle protein. According to the invention, the preferentially designed immunogenic peptide fragment of the human sphingosine 1-phosphate receptor 2 is coupled with the Qbeta-2aa bacteriophage virus-like particle protein carrier to successfully prepare the carrier vaccine. The immunogenic vector vaccine can be used for preparing drugs for preventing and treating abdominal aortic aneurysm.

The invention discloses a co-fermentation method based on sphingomonas and a rhodiola rosea extracting solution and application of a product, and belongs to the technical field of microorganisms. By utilizing a special rich enzyme system of sphingomonas and combining with an early-stage composite enzymolysis process and synergistic effect, the wall breaking efficiency of rhodiola rosea cell walls and the conversion degree of active components of rhodiola rosea are greatly improved, the bottlenecks of low efficiency and low bioavailability of a traditional rhodiola rosea extraction method are successfully overcome, and the rhodiola rosea extraction method is suitable for industrial production. The functional components such as salidroside and flavone can be fully released and converted. Secondly, different from non-symbiotic bacteria (such as lactic acid bacteria and saccharomycetes) which are commonly used for fermentation in the prior art, a potential symbiotic bacterium of sphingomonas XJL-TP07 from a special environment is innovatively selected and has better metabolic suitability with the rhodiola rosea component, the fermentation process is milder and safer (no irritant is produced), and the rhodiola rosea fermentation method is suitable for large-scale production. And a synergistic interaction effect can be generated through a unique biotransformation path.

The present invention provides a combination of superior bacteria for liquefying and degrading food waste and its application. The combination includes Pseudomonas aglycones, Bacillus alcaligenes faecalis, Bacillus amyloliquefaciens, Serratia marcescens, Sphingobacillus suis, Bacillus subtilis, Achromobacter xylosoxidans, Bacillus cereus, Sphingobacillus fusoni, Oligotrophomonas maltophilia, and Sphingobacillus humus. The combination of bacteria can effectively reduce the amount of food waste, and the maximum reduction can be 14.66%, and the COD removal rate can reach 98.19%. The combination of bacteria of the present invention can efficiently degrade and liquefy food waste, and can achieve a high reduction rate and liquefaction effect, which is conducive to the absorption and utilization of nutrients in the subsequent food waste biological resource conversion process.

The invention relates to the technical field of pharmaceutical preparations, and discloses a biological beauty skin care product containing nicotinamide adenine dinucleotide (NAD < + >) and cell exosomes, and the biological beauty skin care product comprises the following components by weight: 10-50 parts of a pH responsive nicotinamide mononucleotide liposome; 1 to 10 parts of cell exosome sustained release microspheres; 0.1 to 2 parts of ergothioneine; 0.1 to 1 part of copper peptide; 1 to 10 parts of phytosphingosine; 0.01 to 0.5 part of water-soluble fullerene; 1-8 parts of 1, 2-pentanediol as a transdermal enhancer; 0.05 to 0.5 part of acetylated polypeptide-8; wherein the particle size of the pH response type nano-liposome is 50-200 nm, and the particle size of the hyaluronic acid microspheres is 100-500 nm, so that the skin health is promoted, and the metabolic function is regulated.

The invention relates to the technical field of microbial engineering, and particularly discloses a compound microbial agent for treating cyanobacterial bloom, bacillus velezensis provided by the invention destroys algae cells and inhibits the activity of related biological enzymes by secreting heterocyclic algal killing active substances, and simultaneously causes the leakage of cellular contents (such as algal toxins, polysaccharides and lipids); an extracellular enzyme system (such as alkaline phosphatase and beta-glucosidase) of the sphingomonas decomposes free algal toxins into low-toxicity linear peptide fragments and hydrolyzes beta-1, 4-glucosidic bonds in EPS at the same time, so that algae-derived organic fragments are converted into small-molecular soluble substances, and a substrate is provided for follow-up metabolism of rhodococcus; lipase and esterase secreted by rhodococcus can hydrolyze triglyceride and waxy components, fatty acid is converted into energy and a biosurfactant through a beta-oxidation pathway, and hydrophilicity of frond fragments is enhanced to promote overall degradation efficiency.

The invention relates to an artificial biological crust material as well as a preparation method and application thereof. Specifically, the artificial biological crust material comprises the following components: kaolin, a polymer material and sphingomonas. In the soil matrix, the addition amount of the components in parts by mass per square meter is as follows: 1.0-6.0 g / m < 2 > of kaolin, 0.3-2.5 kg / m < 2 > of polymer material and 0.02-0.2 g / m < 2 > of sphingomonas by dry weight. The kaolin, the sphingomonas and the polymer are used for synergistically modifying the artificial biological crust material, and the thickness and the microscopic compactness of the biological crust are remarkably improved through synergic modification.

The invention belongs to the technical field of biology, and relates to a heavy metal tolerant sphingomonas strain 1PPS-Zn1 and application thereof, the heavy metal tolerant sphingomonas strain 1PPS-Zn1 is preserved in China Center for Type Culture Collection on March 20, 2025, the preservation number is CCTCC NO: M 2025544, the sphingomonas strain 1PPS-Zn1 can adsorb and tolerate high-concentration zinc, the highest tolerable concentration reaches 80 mmol / L, and the sphingomonas strain 1PPS-Zn1 also has the capabilities of producing IAA, fixing nitrogen and promoting plant growth, and can be applied to the field of heavy metal tolerant plants. And the absorption, enrichment and transfer capabilities of the black nightshade to heavy metal zinc in the soil can be improved, finally, the remediation efficiency of the zinc-polluted soil is improved, a new way is provided for soil remediation and ecological environment remediation, and the method has wide development and application prospects.

The present invention provides A ceramide compound, comprising: a structure of general formula I or an enantiomer or diastereomer of general formula I:in which:R1 is selected from the condensation residues of ferulic acid, sinapic acid, abscisic acid, sotretinoin, tretinoin, fusidic acid, royal jelly acid, sorbic acid, caffeic acid or trans-cinnamic acid;R2 is selected from one of the following structures:—C15H29, —C15H31, —C15H27, —CHOHC14H27, —CHOHC14H29.The present disclosure also provides a ceramide compound containing conjugated carboxylic acid, preparation method therefor and application thereof. The invention reacts functional carboxylic acid with sphingosine base to obtain a class of ceramide compound with novel structure. The introduction of physiologically active molecular fragments of functional carboxylic acids into ceramides will enhance the original efficacy of this type of molecules and also improve the physical and chemical properties of ceramide compound. For example, improving the antioxidant properties of ceramide compound.

The invention provides a saccharomyces cerevisiae strain with high phytosphingosine production. On the basis of the saccharomyces cerevisiae strain with high phytosphingosine production, the invention constructs an engineering saccharomyces cerevisiae strain for efficiently synthesizing phytosphingosine, reduces the expression of proteins encoded by LCB4, SHM2, CHA1, ORM2, ELO3 and SHM1 genes, and increases the expression of proteins encoded by TSC10, SYR2 and LCB2 genes by using a constitutive promoter, and the phytosphingosine shake flask amount reaches 9.52 mg / g dry weight, and then increases the expression of proteins encoded by the HAC1 gene by using a constitutive promoter, and the phytosphingosine shake flask amount is further increased by 4.34 times to 41.33 mg / g dry weight, and the phytosphingosine production reaches the highest production of shake flask fermentation reported so far. The production of phytosphingosine produced by fermentation in a 5L fermenter reaches 150.54 mg / g dry weight, and the titer is 2817 mg / L.

The invention belongs to the field of cosmetics, and particularly discloses a stable soothing anti-aging cosmetic composition as well as a preparation method and application thereof. The cosmetic composition comprises a phase A, a phase B, a phase C and a phase D, the phase A comprises an emollient and a powder dispersant; the phase B comprises an emollient and an active component; the phase C comprises a thickening agent, an emulsifying agent, an emollient, a film-forming agent and a skin feeling conditioning agent; the phase D comprises water, a humectant, an antioxidant, an anti-freezing agent and a thickening agent; the active ingredients comprise at least one of phytosphingosine, ceramide NP, batyl alcohol, a glycyrrhiza inflate extract and licochalcone A. The invention further discloses a preparation method of the pharmaceutical composition. The cosmetic composition is good in stability and good in moisturizing, soothing and anti-aging effects, and has an unexpected synergistic technical effect.

The invention discloses a functional lipid compound for nano-liposome wrapping and a preparation method, and relates to the technical field of functional lipid compounds, the functional lipid compound comprises (a) a polyphenol-lipid covalent conjugate which is composed of a polyphenol active unit selected from curcumin, dihydroquercetin or derivatives thereof, and (b) a lipid covalent conjugate which is composed of a polyphenols active unit selected from curcumin, dihydroquercetin or derivatives thereof and a polyphenols active unit selected from curcumin, dihydroquercetin or derivatives thereof; the conjugate is covalently linked to a phosphatidyl ethanolamine or cholesterol skeleton through a cleavable linking group, and the conjugate can be integrated into a nano-liposome membrane and has the functions of structural support and biological activity; (b) a microenvironment-responsive functional phospholipid, which is a phospholipid derivative having a functional group responsive to an inflammatory microenvironment specific stimulus modified on a hydrophilic head group or a hydrophobic chain of a phospholipid molecule, the specific stimulus being selected from local pH < = 6.5, reactive oxygen species (ROS) concentration > = 10 [mu] M or matrix metalloproteinase MMP-2 / 9 overexpression; and (c) a bionic exosome membrane lipid component, wherein the bionic exosome membrane lipid component comprises sphingosine-based glycolipid or sulfated cholesterol separated from the plant-derived exosome.

The present invention relates to Compounds of Formula I: and pharmaceutically acceptable salts or prodrug thereof. The present invention also relates to compositions comprising at least one compound of Formula I, and methods of using the compounds of Formula I for treatment or prophylaxis of lysosomal storage diseases, neurodegenerative disease, cystic disease, cancer, or a diseases or disorders associated with elevated levels of glucosylceramide (GlcCer), glucosylsphingosine (GlcSph) and / or other glucosylceramide-based glycosphingolipids (GSLs).

The present application provides a kind of cosmetic composition for acne and its preparation method, it is related to the technical field of cosmetics.The cosmetic composition comprises by mass percentage 0.01-2.0% of acacia gum, 0.01-2.0% of sphingomonad ferment product extract, 0.01-10% of exfoliating agent, 0.01-1.0% of oil control agent, 0.001-0.005% of bacteriostatic agent, 0.01-2.0% of anti-inflammatory agent and 0.01-2.0% of soothing and repairing agent; the balance is cosmetic base; wherein the exfoliating agent includes one or more of coated multi-fruit acid, coated azelaic acid and coated salicylic acid; the anti-inflammatory agent includes 100 and 1609; and the soothing and repairing agent includes 100, which solves the technical problems of difficult thickening, insufficient mildness and insufficient refreshing after absorption of acne-removing products.

The invention provides a metabolic marker for auxiliary diagnosis of schizophrenia, a construction method and application. Wherein the metabolic marker comprises lysophosphatidylcholine (LPC) (17: 0 / 0: 0), oleamide, lysophosphatidyl ethanolamine LysoPE (18: 0 / 0: 0), palmitamide, lysophosphatidyl ethanolamine LysoPE (0: 0 / 20: 4), lysophosphatidyl ethanolamine LysoPE (22: 6 / 0: 0), p-methylphenol sulfate, lactic acid, 3-hydroxybutyric acid, lysophosphatidyl ethanolamine LysoPE (18: 2 / 0: 0), lysophosphatidyl ethanolamine LysoPE (16: 0 / 0: 0) and sphingosine 1-phosphate. By utilizing the metabolic marker, auxiliary diagnosis of schizophrenia can be rapidly and accurately completed in the early stage of disease attack.

202300151 Foreign Filing 48 Abstract The invention relates to compositions comprising (pseudo-) ceramide and / or sphingoid base and a solvent composed of at least two components, a process for producing a formulation (pseudo-) ceramide and / or sphingoid base and the use of at least one of said composed solvent for 5 stabilization of (pseudo-) ceramide and / or sphingoid base containing composition.

Provided is a novel agent which can induce an effect of inhibiting the expression of an itch-related gene and which can be mainly applied to human skin or the like. The agent for inhibiting pruritus according to the present invention contains sphingosine base.

The invention discloses an anti-aging and anti-allergy spray suitable for pregnant women based on micro-ecology-barrier-immune synergistic repair and a preparation method of the anti-aging and anti-allergy spray. The spray is prepared from 2.00 percent to 4.00 percent of hydrolyzed quinoa protein, 1.00 percent to 5.00 percent of lactobacillus / soybean milk fermentation filtrate, 0.30 percent to 1.00 percent of radix stellariae extract, 0.20 percent to 1.50 percent of perilla leaf extract, 0.01 percent to 0.50 percent of three-layer liposome tetrahydrocurcumin, 1.00 percent to 10.00 percent of Tibetan plateau freeze-dried tricholoma matsutake extracting solution, an active peptide combination and a ceramide compound. According to a quadruple synergistic mechanism, (1) the pH value of the fermentation filtrate is adjusted to 5.3, and the fermentation filtrate and the hydrolyzed quinoa extract synergistically construct a micro-ecological foundation; (2) repairing the barrier by ceramide NP, cholesterol, phytosphingosine and the like; (3) multiple anti-allergy effects of radices stellariae dichotomae, purple perilla, asiaticoside and dipotassium glycyrrhizinate; and (4) liposome curcumin, glabridin liposome and active peptide cooperate to resist aging, and a'micro-ecological balance-barrier reconstruction-immunosuppression-targeted anti-aging 'quadruple synergistic effect system is constructed. The preparation method comprises the steps of A-phase / B-phase preparation, emulsification, active addition and sterile filling, and the skin requirements in the pregnancy period and the lactation period are met.

The invention relates to the technical field of biology, in particular to a biological substrate modification composition, a preparation as well as a preparation method and application of the biological substrate modification composition. The biological substrate modification composition provided by the invention is prepared from the following components: bacillus subtilis YXSY-01 bacterial powder, sphingomonas aquatic bacteria HD-01 bacterial liquid, bentonite, oyster shell powder, attapulgite, zeolite powder, fish bone meal of raw fish and water. The biological substrate modification composition is prepared from the following raw materials in parts by weight: 20-30% of corn starch, 20-30% of corn starch, 20-30% of corn starch, 20-30% of corn starch, 20-30% of corn starch, 20-30% of corn starch, 20-30% of corn starch, 20-30% of corn starch, 20-30% of corn starch, 20-30% of corn starch and 20-30% of corn starch. The biological substrate modification composition and the preparation provided by the invention have the capabilities of degrading faeces of bred animals and reducing the content of nitrite. The composition and the preparation can reduce the accumulation of excrement of cultured animals in a culture pond, reduce the nitrite content, purify the water quality of the culture pond and reduce the morbidity of the cultured animals.

In some embodiments, the invention provides a method for identifying an agent beneficial to treat a patient with inflammatory bowel disease comprising: a) determining a status of an intestinal barrier in the patient; and b) categorizing the status as severe dysfunction or moderate dysfunction, wherein a patient categorized as having severe dysfunction is identified as a patient who will benefit from treatment with an agent selected from the group consisting of an anti-TNF agent and / or an anti-IL-12 / 23 agent, and a patient categorized as having moderate dysfunction is identified as a patient who will benefit from treatment with an anti-integrin agent, an anti-janus kinase agent, and / or and a sphingosine-1-phosphate receptor agonist agent.